This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGTGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.82 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 86,114 (2.9%) Reads that were too short: 9,079 (0.3%) Reads written (passing filters): 77,035 (2.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 19,878,374 bp (2.2%) === Adapter 1 === Sequence: AGTGTT; Type: regular 5'; Length: 6; Trimmed: 86114 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 473 734.8 0 473 7 44483 734.8 0 44483 8 177 734.8 0 177 9 68 734.8 0 68 10 62 734.8 0 62 11 43 734.8 0 43 12 46 734.8 0 46 13 53 734.8 0 53 14 63 734.8 0 63 15 46 734.8 0 46 16 43 734.8 0 43 17 44 734.8 0 44 18 50 734.8 0 50 19 50 734.8 0 50 20 48 734.8 0 48 21 79 734.8 0 79 22 65 734.8 0 65 23 63 734.8 0 63 24 66 734.8 0 66 25 57 734.8 0 57 26 55 734.8 0 55 27 59 734.8 0 59 28 175 734.8 0 175 29 88 734.8 0 88 30 247 734.8 0 247 31 84 734.8 0 84 32 3867 734.8 0 3867 33 93 734.8 0 93 34 52 734.8 0 52 35 60 734.8 0 60 36 32 734.8 0 32 37 62 734.8 0 62 38 53 734.8 0 53 39 44 734.8 0 44 40 53 734.8 0 53 41 69 734.8 0 69 42 49 734.8 0 49 43 48 734.8 0 48 44 60 734.8 0 60 45 61 734.8 0 61 46 62 734.8 0 62 47 52 734.8 0 52 48 67 734.8 0 67 49 59 734.8 0 59 50 34 734.8 0 34 51 63 734.8 0 63 52 56 734.8 0 56 53 47 734.8 0 47 54 53 734.8 0 53 55 60 734.8 0 60 56 71 734.8 0 71 57 79 734.8 0 79 58 73 734.8 0 73 59 77 734.8 0 77 60 51 734.8 0 51 61 63 734.8 0 63 62 125 734.8 0 125 63 179 734.8 0 179 64 135 734.8 0 135 65 92 734.8 0 92 66 127 734.8 0 127 67 136 734.8 0 136 68 1757 734.8 0 1757 69 4947 734.8 0 4947 70 818 734.8 0 818 71 57 734.8 0 57 72 70 734.8 0 70 73 52 734.8 0 52 74 50 734.8 0 50 75 63 734.8 0 63 76 49 734.8 0 49 77 109 734.8 0 109 78 75 734.8 0 75 79 155 734.8 0 155 80 160 734.8 0 160 81 94 734.8 0 94 82 72 734.8 0 72 83 67 734.8 0 67 84 91 734.8 0 91 85 69 734.8 0 69 86 68 734.8 0 68 87 95 734.8 0 95 88 129 734.8 0 129 89 59 734.8 0 59 90 81 734.8 0 81 91 63 734.8 0 63 92 76 734.8 0 76 93 138 734.8 0 138 94 212 734.8 0 212 95 181 734.8 0 181 96 216 734.8 0 216 97 115 734.8 0 115 98 130 734.8 0 130 99 992 734.8 0 992 100 1564 734.8 0 1564 101 706 734.8 0 706 102 52 734.8 0 52 103 48 734.8 0 48 104 50 734.8 0 50 105 42 734.8 0 42 106 38 734.8 0 38 107 47 734.8 0 47 108 54 734.8 0 54 109 61 734.8 0 61 110 72 734.8 0 72 111 64 734.8 0 64 112 70 734.8 0 70 113 75 734.8 0 75 114 72 734.8 0 72 115 61 734.8 0 61 116 47 734.8 0 47 117 75 734.8 0 75 118 84 734.8 0 84 119 59 734.8 0 59 120 53 734.8 0 53 121 57 734.8 0 57 122 56 734.8 0 56 123 73 734.8 0 73 124 104 734.8 0 104 125 100 734.8 0 100 126 85 734.8 0 85 127 109 734.8 0 109 128 100 734.8 0 100 129 121 734.8 0 121 130 121 734.8 0 121 131 119 734.8 0 119 132 168 734.8 0 168 133 92 734.8 0 92 134 83 734.8 0 83 135 80 734.8 0 80 136 67 734.8 0 67 137 70 734.8 0 70 138 61 734.8 0 61 139 91 734.8 0 91 140 58 734.8 0 58 141 77 734.8 0 77 142 65 734.8 0 65 143 106 734.8 0 106 144 87 734.8 0 87 145 212 734.8 0 212 146 1359 734.8 0 1359 147 130 734.8 0 130 148 90 734.8 0 90 149 69 734.8 0 69 150 66 734.8 0 66 151 58 734.8 0 58 152 62 734.8 0 62 153 93 734.8 0 93 154 64 734.8 0 64 155 54 734.8 0 54 156 63 734.8 0 63 157 58 734.8 0 58 158 68 734.8 0 68 159 77 734.8 0 77 160 78 734.8 0 78 161 64 734.8 0 64 162 50 734.8 0 50 163 66 734.8 0 66 164 62 734.8 0 62 165 68 734.8 0 68 166 79 734.8 0 79 167 257 734.8 0 257 168 154 734.8 0 154 169 110 734.8 0 110 170 61 734.8 0 61 171 53 734.8 0 53 172 52 734.8 0 52 173 75 734.8 0 75 174 97 734.8 0 97 175 75 734.8 0 75 176 90 734.8 0 90 177 64 734.8 0 64 178 64 734.8 0 64 179 62 734.8 0 62 180 69 734.8 0 69 181 65 734.8 0 65 182 67 734.8 0 67 183 78 734.8 0 78 184 72 734.8 0 72 185 54 734.8 0 54 186 48 734.8 0 48 187 69 734.8 0 69 188 56 734.8 0 56 189 47 734.8 0 47 190 68 734.8 0 68 191 121 734.8 0 121 192 177 734.8 0 177 193 230 734.8 0 230 194 257 734.8 0 257 195 153 734.8 0 153 196 149 734.8 0 149 197 158 734.8 0 158 198 351 734.8 0 351 199 267 734.8 0 267 200 170 734.8 0 170 201 100 734.8 0 100 202 118 734.8 0 118 203 147 734.8 0 147 204 92 734.8 0 92 205 97 734.8 0 97 206 129 734.8 0 129 207 95 734.8 0 95 208 72 734.8 0 72 209 73 734.8 0 73 210 89 734.8 0 89 211 134 734.8 0 134 212 156 734.8 0 156 213 132 734.8 0 132 214 110 734.8 0 110 215 89 734.8 0 89 216 92 734.8 0 92 217 79 734.8 0 79 218 63 734.8 0 63 219 200 734.8 0 200 220 249 734.8 0 249 221 153 734.8 0 153 222 105 734.8 0 105 223 84 734.8 0 84 224 95 734.8 0 95 225 67 734.8 0 67 226 67 734.8 0 67 227 78 734.8 0 78 228 80 734.8 0 80 229 67 734.8 0 67 230 68 734.8 0 68 231 89 734.8 0 89 232 183 734.8 0 183 233 322 734.8 0 322 234 293 734.8 0 293 235 226 734.8 0 226 236 132 734.8 0 132 237 106 734.8 0 106 238 153 734.8 0 153 239 101 734.8 0 101 240 120 734.8 0 120 241 95 734.8 0 95 242 135 734.8 0 135 243 126 734.8 0 126 244 85 734.8 0 85 245 69 734.8 0 69 246 69 734.8 0 69 247 75 734.8 0 75 248 60 734.8 0 60 249 69 734.8 0 69 250 61 734.8 0 61 251 59 734.8 0 59 252 79 734.8 0 79 253 133 734.8 0 133 254 123 734.8 0 123 255 92 734.8 0 92 256 101 734.8 0 101 257 63 734.8 0 63 258 60 734.8 0 60 259 52 734.8 0 52 260 72 734.8 0 72 261 104 734.8 0 104 262 90 734.8 0 90 263 69 734.8 0 69 264 71 734.8 0 71 265 69 734.8 0 69 266 96 734.8 0 96 267 77 734.8 0 77 268 117 734.8 0 117 269 60 734.8 0 60 270 45 734.8 0 45 271 50 734.8 0 50 272 65 734.8 0 65 273 98 734.8 0 98 274 75 734.8 0 75 275 55 734.8 0 55 276 53 734.8 0 53 277 40 734.8 0 40 278 48 734.8 0 48 279 57 734.8 0 57 280 56 734.8 0 56 281 42 734.8 0 42 282 66 734.8 0 66 283 73 734.8 0 73 284 80 734.8 0 80 285 55 734.8 0 55 286 51 734.8 0 51 287 47 734.8 0 47 288 48 734.8 0 48 289 43 734.8 0 43 290 40 734.8 0 40 291 60 734.8 0 60 292 83 734.8 0 83 293 58 734.8 0 58 294 40 734.8 0 40 295 30 734.8 0 30 296 60 734.8 0 60 297 53 734.8 0 53 298 44 734.8 0 44 299 44 734.8 0 44 300 68 734.8 0 68 301 116 734.8 0 116 Finished in 3.45 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 77,035 Reads with adapters: 44,742 (58.1%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 44,725 (58.1%) Total basepairs processed: 19,878,374 bp Total written (filtered): 12,384,028 bp (62.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 44742 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 13 0.0 3 0 0 13 16 464 0.0 3 0 442 13 9 17 44051 0.0 3 42707 1134 171 39 18 75 0.0 3 4 66 5 19 44 0.0 3 0 0 33 11 20 42 0.0 3 0 0 0 42 32 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 100 6 0.0 3 0 0 0 6 102 1 0.0 3 0 0 0 1 112 4 0.0 3 0 0 1 3 115 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 159 4 0.0 3 0 0 0 4 170 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.58 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 44,725 Reads with adapters: 2,591 (5.8%) Reads written (passing filters): 44,725 (100.0%) Total basepairs processed: 12,384,028 bp Total written (filtered): 12,376,221 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2591 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.6% C: 45.7% G: 4.8% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2566 698.8 0 2566 4 16 174.7 0 16 5 9 43.7 0 9