This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.18 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 343,579 (11.4%) Reads that were too short: 120,139 (4.0%) Reads written (passing filters): 223,440 (7.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 42,686,655 bp (4.7%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 343579 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 437 734.8 0 437 7 46027 734.8 0 46027 8 100 734.8 0 100 9 4361 734.8 0 4361 10 25 734.8 0 25 11 42 734.8 0 42 12 37 734.8 0 37 13 19 734.8 0 19 14 17 734.8 0 17 15 34 734.8 0 34 16 20 734.8 0 20 17 10 734.8 0 10 18 19 734.8 0 19 19 14 734.8 0 14 20 20 734.8 0 20 21 7 734.8 0 7 22 12 734.8 0 12 23 11 734.8 0 11 24 13 734.8 0 13 25 12 734.8 0 12 26 18 734.8 0 18 27 22 734.8 0 22 28 26 734.8 0 26 29 20 734.8 0 20 30 14 734.8 0 14 31 18 734.8 0 18 32 25 734.8 0 25 33 17 734.8 0 17 34 11 734.8 0 11 35 25 734.8 0 25 36 25 734.8 0 25 37 15 734.8 0 15 38 46 734.8 0 46 39 77 734.8 0 77 40 265 734.8 0 265 41 25 734.8 0 25 42 15 734.8 0 15 43 24 734.8 0 24 44 17 734.8 0 17 45 13 734.8 0 13 46 10 734.8 0 10 47 21 734.8 0 21 48 9 734.8 0 9 49 26 734.8 0 26 50 20 734.8 0 20 51 20 734.8 0 20 52 14 734.8 0 14 53 22 734.8 0 22 54 104 734.8 0 104 55 19 734.8 0 19 56 21 734.8 0 21 57 13 734.8 0 13 58 57 734.8 0 57 59 55 734.8 0 55 60 23 734.8 0 23 61 24 734.8 0 24 62 28 734.8 0 28 63 251 734.8 0 251 64 13 734.8 0 13 65 19 734.8 0 19 66 13 734.8 0 13 67 15 734.8 0 15 68 19 734.8 0 19 69 261 734.8 0 261 70 32 734.8 0 32 71 28 734.8 0 28 72 27 734.8 0 27 73 194 734.8 0 194 74 74 734.8 0 74 75 54 734.8 0 54 76 44 734.8 0 44 77 149 734.8 0 149 78 434 734.8 0 434 79 747 734.8 0 747 80 1820 734.8 0 1820 81 1436 734.8 0 1436 82 1651 734.8 0 1651 83 2142 734.8 0 2142 84 1841 734.8 0 1841 85 1474 734.8 0 1474 86 2060 734.8 0 2060 87 2301 734.8 0 2301 88 988 734.8 0 988 89 536 734.8 0 536 90 583 734.8 0 583 91 1005 734.8 0 1005 92 2275 734.8 0 2275 93 906 734.8 0 906 94 1327 734.8 0 1327 95 1093 734.8 0 1093 96 745 734.8 0 745 97 639 734.8 0 639 98 554 734.8 0 554 99 508 734.8 0 508 100 1649 734.8 0 1649 101 2076 734.8 0 2076 102 1261 734.8 0 1261 103 1142 734.8 0 1142 104 775 734.8 0 775 105 501 734.8 0 501 106 398 734.8 0 398 107 412 734.8 0 412 108 1035 734.8 0 1035 109 1340 734.8 0 1340 110 1482 734.8 0 1482 111 1127 734.8 0 1127 112 1409 734.8 0 1409 113 1065 734.8 0 1065 114 819 734.8 0 819 115 1064 734.8 0 1064 116 1575 734.8 0 1575 117 1803 734.8 0 1803 118 1928 734.8 0 1928 119 1722 734.8 0 1722 120 1869 734.8 0 1869 121 1791 734.8 0 1791 122 1709 734.8 0 1709 123 1369 734.8 0 1369 124 1316 734.8 0 1316 125 1412 734.8 0 1412 126 1235 734.8 0 1235 127 991 734.8 0 991 128 1098 734.8 0 1098 129 1254 734.8 0 1254 130 1272 734.8 0 1272 131 1988 734.8 0 1988 132 1349 734.8 0 1349 133 1076 734.8 0 1076 134 1107 734.8 0 1107 135 1086 734.8 0 1086 136 1312 734.8 0 1312 137 1380 734.8 0 1380 138 1638 734.8 0 1638 139 1589 734.8 0 1589 140 1175 734.8 0 1175 141 1187 734.8 0 1187 142 1616 734.8 0 1616 143 1346 734.8 0 1346 144 1136 734.8 0 1136 145 1573 734.8 0 1573 146 1589 734.8 0 1589 147 1545 734.8 0 1545 148 1470 734.8 0 1470 149 1200 734.8 0 1200 150 1371 734.8 0 1371 151 1298 734.8 0 1298 152 1483 734.8 0 1483 153 1555 734.8 0 1555 154 1247 734.8 0 1247 155 1420 734.8 0 1420 156 1161 734.8 0 1161 157 1313 734.8 0 1313 158 1473 734.8 0 1473 159 1379 734.8 0 1379 160 1437 734.8 0 1437 161 1139 734.8 0 1139 162 1288 734.8 0 1288 163 1066 734.8 0 1066 164 1097 734.8 0 1097 165 859 734.8 0 859 166 2002 734.8 0 2002 167 4028 734.8 0 4028 168 1651 734.8 0 1651 169 1313 734.8 0 1313 170 2349 734.8 0 2349 171 2460 734.8 0 2460 172 1735 734.8 0 1735 173 1121 734.8 0 1121 174 1093 734.8 0 1093 175 1367 734.8 0 1367 176 1271 734.8 0 1271 177 1413 734.8 0 1413 178 1378 734.8 0 1378 179 1175 734.8 0 1175 180 1529 734.8 0 1529 181 2128 734.8 0 2128 182 2220 734.8 0 2220 183 2243 734.8 0 2243 184 1659 734.8 0 1659 185 1354 734.8 0 1354 186 1179 734.8 0 1179 187 1333 734.8 0 1333 188 1417 734.8 0 1417 189 1262 734.8 0 1262 190 1118 734.8 0 1118 191 1132 734.8 0 1132 192 1191 734.8 0 1191 193 1090 734.8 0 1090 194 1017 734.8 0 1017 195 1209 734.8 0 1209 196 1000 734.8 0 1000 197 1499 734.8 0 1499 198 1746 734.8 0 1746 199 1486 734.8 0 1486 200 1777 734.8 0 1777 201 949 734.8 0 949 202 1088 734.8 0 1088 203 1229 734.8 0 1229 204 1314 734.8 0 1314 205 1323 734.8 0 1323 206 1214 734.8 0 1214 207 1194 734.8 0 1194 208 1025 734.8 0 1025 209 941 734.8 0 941 210 1022 734.8 0 1022 211 1173 734.8 0 1173 212 1137 734.8 0 1137 213 2006 734.8 0 2006 214 2197 734.8 0 2197 215 1374 734.8 0 1374 216 1016 734.8 0 1016 217 1129 734.8 0 1129 218 1447 734.8 0 1447 219 1537 734.8 0 1537 220 1213 734.8 0 1213 221 903 734.8 0 903 222 1034 734.8 0 1034 223 1296 734.8 0 1296 224 1214 734.8 0 1214 225 933 734.8 0 933 226 947 734.8 0 947 227 938 734.8 0 938 228 1153 734.8 0 1153 229 1057 734.8 0 1057 230 934 734.8 0 934 231 904 734.8 0 904 232 976 734.8 0 976 233 1078 734.8 0 1078 234 969 734.8 0 969 235 954 734.8 0 954 236 834 734.8 0 834 237 969 734.8 0 969 238 1261 734.8 0 1261 239 1215 734.8 0 1215 240 1061 734.8 0 1061 241 963 734.8 0 963 242 1067 734.8 0 1067 243 1056 734.8 0 1056 244 997 734.8 0 997 245 937 734.8 0 937 246 1195 734.8 0 1195 247 1063 734.8 0 1063 248 1226 734.8 0 1226 249 1405 734.8 0 1405 250 1213 734.8 0 1213 251 1061 734.8 0 1061 252 1465 734.8 0 1465 253 1091 734.8 0 1091 254 981 734.8 0 981 255 883 734.8 0 883 256 918 734.8 0 918 257 821 734.8 0 821 258 786 734.8 0 786 259 707 734.8 0 707 260 754 734.8 0 754 261 918 734.8 0 918 262 1492 734.8 0 1492 263 1520 734.8 0 1520 264 1098 734.8 0 1098 265 848 734.8 0 848 266 928 734.8 0 928 267 2728 734.8 0 2728 268 6057 734.8 0 6057 269 2984 734.8 0 2984 270 1172 734.8 0 1172 271 877 734.8 0 877 272 1312 734.8 0 1312 273 1188 734.8 0 1188 274 886 734.8 0 886 275 878 734.8 0 878 276 761 734.8 0 761 277 901 734.8 0 901 278 1045 734.8 0 1045 279 1065 734.8 0 1065 280 1295 734.8 0 1295 281 1689 734.8 0 1689 282 870 734.8 0 870 283 928 734.8 0 928 284 879 734.8 0 879 285 718 734.8 0 718 286 920 734.8 0 920 287 894 734.8 0 894 288 776 734.8 0 776 289 690 734.8 0 690 290 1118 734.8 0 1118 291 1327 734.8 0 1327 292 1532 734.8 0 1532 293 1093 734.8 0 1093 294 893 734.8 0 893 295 822 734.8 0 822 296 950 734.8 0 950 297 1486 734.8 0 1486 298 1669 734.8 0 1669 299 1912 734.8 0 1912 300 1718 734.8 0 1718 301 1504 734.8 0 1504 Finished in 8.42 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 223,440 Reads with adapters: 46,484 (20.8%) Reads that were too short: 57 (0.0%) Reads written (passing filters): 46,427 (20.8%) Total basepairs processed: 42,686,655 bp Total written (filtered): 12,713,845 bp (29.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 46484 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 18 0.0 3 0 0 16 0 2 19 517 0.0 3 0 508 7 0 2 20 45672 0.0 4 45046 604 18 3 1 21 128 0.0 4 27 98 1 0 2 22 8 0.0 4 0 0 8 23 15 0.0 4 0 1 0 13 1 24 3 0.0 4 0 1 0 1 1 25 2 0.0 4 1 0 0 0 1 28 2 0.0 4 0 1 1 30 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 48 17 0.0 4 0 0 0 0 17 49 2 0.0 4 0 0 0 0 2 50 1 0.0 4 0 0 0 0 1 51 2 0.0 4 0 0 0 0 2 52 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 3 0.0 4 0 0 0 0 3 66 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 2 0.0 4 0 0 0 0 2 74 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 81 2 0.0 4 0 0 0 0 2 84 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 111 22 0.0 4 0 0 0 19 3 113 1 0.0 4 0 0 0 0 1 114 2 0.0 4 0 0 0 0 2 116 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 134 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 211 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.57 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 46,427 Reads with adapters: 1,417 (3.1%) Reads written (passing filters): 46,427 (100.0%) Total basepairs processed: 12,713,845 bp Total written (filtered): 12,696,082 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1417 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 0.8% G: 97.7% T: 0.6% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 725.4 0 3 4 2 181.4 0 2 5 2 45.3 0 2 6 2 11.3 0 2 10 10 0.0 1 0 10 11 1380 0.0 1 7 1373 12 8 0.0 1 0 8 152 2 0.0 1 0 2 191 1 0.0 1 0 1 259 2 0.0 1 2 260 1 0.0 1 1 261 1 0.0 1 1 270 2 0.0 1 2 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.