This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.20 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 124,498 (4.1%) Reads that were too short: 86,123 (2.9%) Reads written (passing filters): 38,375 (1.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 7,710,776 bp (0.9%) === Adapter 1 === Sequence: AACCGA; Type: regular 5'; Length: 6; Trimmed: 124498 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 132 734.8 0 132 7 133 734.8 0 133 8 10534 734.8 0 10534 9 28 734.8 0 28 10 15 734.8 0 15 11 67 734.8 0 67 12 16 734.8 0 16 13 16 734.8 0 16 14 13 734.8 0 13 15 10 734.8 0 10 16 18 734.8 0 18 17 21 734.8 0 21 18 14 734.8 0 14 19 7 734.8 0 7 20 23 734.8 0 23 21 12 734.8 0 12 22 12 734.8 0 12 23 28 734.8 0 28 24 11 734.8 0 11 25 28 734.8 0 28 26 23 734.8 0 23 27 17 734.8 0 17 28 9 734.8 0 9 29 18 734.8 0 18 30 11 734.8 0 11 31 10 734.8 0 10 32 19 734.8 0 19 33 12 734.8 0 12 34 21 734.8 0 21 35 14 734.8 0 14 36 7 734.8 0 7 37 14 734.8 0 14 38 18 734.8 0 18 39 19 734.8 0 19 40 10 734.8 0 10 41 12 734.8 0 12 42 16 734.8 0 16 43 12 734.8 0 12 44 13 734.8 0 13 45 19 734.8 0 19 46 15 734.8 0 15 47 16 734.8 0 16 48 8 734.8 0 8 49 14 734.8 0 14 50 18 734.8 0 18 51 16 734.8 0 16 52 13 734.8 0 13 53 19 734.8 0 19 54 15 734.8 0 15 55 14 734.8 0 14 56 30 734.8 0 30 57 20 734.8 0 20 58 24 734.8 0 24 59 13 734.8 0 13 60 34 734.8 0 34 61 35 734.8 0 35 62 35 734.8 0 35 63 21 734.8 0 21 64 104 734.8 0 104 65 5 734.8 0 5 66 17 734.8 0 17 67 24 734.8 0 24 68 27 734.8 0 27 69 18 734.8 0 18 70 79 734.8 0 79 71 334 734.8 0 334 72 676 734.8 0 676 73 78 734.8 0 78 74 80 734.8 0 80 75 76 734.8 0 76 76 157 734.8 0 157 77 207 734.8 0 207 78 171 734.8 0 171 79 110 734.8 0 110 80 169 734.8 0 169 81 246 734.8 0 246 82 177 734.8 0 177 83 336 734.8 0 336 84 343 734.8 0 343 85 206 734.8 0 206 86 110 734.8 0 110 87 120 734.8 0 120 88 63 734.8 0 63 89 76 734.8 0 76 90 332 734.8 0 332 91 170 734.8 0 170 92 180 734.8 0 180 93 203 734.8 0 203 94 146 734.8 0 146 95 176 734.8 0 176 96 145 734.8 0 145 97 54 734.8 0 54 98 64 734.8 0 64 99 56 734.8 0 56 100 71 734.8 0 71 101 89 734.8 0 89 102 78 734.8 0 78 103 158 734.8 0 158 104 101 734.8 0 101 105 38 734.8 0 38 106 44 734.8 0 44 107 158 734.8 0 158 108 133 734.8 0 133 109 134 734.8 0 134 110 156 734.8 0 156 111 126 734.8 0 126 112 146 734.8 0 146 113 161 734.8 0 161 114 148 734.8 0 148 115 198 734.8 0 198 116 253 734.8 0 253 117 142 734.8 0 142 118 146 734.8 0 146 119 211 734.8 0 211 120 186 734.8 0 186 121 138 734.8 0 138 122 146 734.8 0 146 123 238 734.8 0 238 124 311 734.8 0 311 125 272 734.8 0 272 126 587 734.8 0 587 127 487 734.8 0 487 128 246 734.8 0 246 129 124 734.8 0 124 130 96 734.8 0 96 131 128 734.8 0 128 132 172 734.8 0 172 133 151 734.8 0 151 134 230 734.8 0 230 135 222 734.8 0 222 136 235 734.8 0 235 137 271 734.8 0 271 138 180 734.8 0 180 139 215 734.8 0 215 140 245 734.8 0 245 141 204 734.8 0 204 142 115 734.8 0 115 143 155 734.8 0 155 144 188 734.8 0 188 145 220 734.8 0 220 146 168 734.8 0 168 147 131 734.8 0 131 148 170 734.8 0 170 149 116 734.8 0 116 150 177 734.8 0 177 151 140 734.8 0 140 152 192 734.8 0 192 153 295 734.8 0 295 154 247 734.8 0 247 155 244 734.8 0 244 156 216 734.8 0 216 157 259 734.8 0 259 158 296 734.8 0 296 159 181 734.8 0 181 160 233 734.8 0 233 161 129 734.8 0 129 162 128 734.8 0 128 163 168 734.8 0 168 164 252 734.8 0 252 165 259 734.8 0 259 166 179 734.8 0 179 167 137 734.8 0 137 168 132 734.8 0 132 169 197 734.8 0 197 170 284 734.8 0 284 171 212 734.8 0 212 172 305 734.8 0 305 173 424 734.8 0 424 174 283 734.8 0 283 175 302 734.8 0 302 176 218 734.8 0 218 177 201 734.8 0 201 178 213 734.8 0 213 179 204 734.8 0 204 180 230 734.8 0 230 181 226 734.8 0 226 182 210 734.8 0 210 183 156 734.8 0 156 184 238 734.8 0 238 185 216 734.8 0 216 186 205 734.8 0 205 187 163 734.8 0 163 188 102 734.8 0 102 189 285 734.8 0 285 190 406 734.8 0 406 191 347 734.8 0 347 192 268 734.8 0 268 193 282 734.8 0 282 194 119 734.8 0 119 195 205 734.8 0 205 196 243 734.8 0 243 197 572 734.8 0 572 198 403 734.8 0 403 199 131 734.8 0 131 200 135 734.8 0 135 201 161 734.8 0 161 202 311 734.8 0 311 203 334 734.8 0 334 204 215 734.8 0 215 205 192 734.8 0 192 206 216 734.8 0 216 207 183 734.8 0 183 208 128 734.8 0 128 209 147 734.8 0 147 210 256 734.8 0 256 211 137 734.8 0 137 212 156 734.8 0 156 213 186 734.8 0 186 214 197 734.8 0 197 215 286 734.8 0 286 216 495 734.8 0 495 217 2936 734.8 0 2936 218 1009 734.8 0 1009 219 289 734.8 0 289 220 147 734.8 0 147 221 212 734.8 0 212 222 432 734.8 0 432 223 538 734.8 0 538 224 237 734.8 0 237 225 312 734.8 0 312 226 388 734.8 0 388 227 372 734.8 0 372 228 594 734.8 0 594 229 4604 734.8 0 4604 230 2718 734.8 0 2718 231 794 734.8 0 794 232 153 734.8 0 153 233 201 734.8 0 201 234 217 734.8 0 217 235 228 734.8 0 228 236 139 734.8 0 139 237 158 734.8 0 158 238 147 734.8 0 147 239 155 734.8 0 155 240 121 734.8 0 121 241 113 734.8 0 113 242 123 734.8 0 123 243 135 734.8 0 135 244 172 734.8 0 172 245 279 734.8 0 279 246 394 734.8 0 394 247 253 734.8 0 253 248 104 734.8 0 104 249 51 734.8 0 51 250 119 734.8 0 119 251 213 734.8 0 213 252 337 734.8 0 337 253 226 734.8 0 226 254 152 734.8 0 152 255 148 734.8 0 148 256 181 734.8 0 181 257 162 734.8 0 162 258 174 734.8 0 174 259 624 734.8 0 624 260 1417 734.8 0 1417 261 553 734.8 0 553 262 387 734.8 0 387 263 457 734.8 0 457 264 251 734.8 0 251 265 287 734.8 0 287 266 525 734.8 0 525 267 377 734.8 0 377 268 543 734.8 0 543 269 203 734.8 0 203 270 233 734.8 0 233 271 293 734.8 0 293 272 208 734.8 0 208 273 215 734.8 0 215 274 167 734.8 0 167 275 212 734.8 0 212 276 187 734.8 0 187 277 347 734.8 0 347 278 462 734.8 0 462 279 532 734.8 0 532 280 299 734.8 0 299 281 145 734.8 0 145 282 178 734.8 0 178 283 312 734.8 0 312 284 633 734.8 0 633 285 622 734.8 0 622 286 508 734.8 0 508 287 1049 734.8 0 1049 288 799 734.8 0 799 289 487 734.8 0 487 290 553 734.8 0 553 291 705 734.8 0 705 292 1440 734.8 0 1440 293 25187 734.8 0 25187 294 15568 734.8 0 15568 295 4081 734.8 0 4081 296 142 734.8 0 142 297 121 734.8 0 121 298 168 734.8 0 168 299 208 734.8 0 208 300 189 734.8 0 189 301 373 734.8 0 373 Finished in 1.45 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 38,375 Reads with adapters: 10,635 (27.7%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 10,622 (27.7%) Total basepairs processed: 7,710,776 bp Total written (filtered): 2,926,873 bp (38.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10635 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 6 16 107 0.0 3 0 91 9 7 17 10399 0.0 3 10151 218 18 12 18 26 0.0 3 0 14 8 4 19 13 0.0 3 2 0 2 9 20 39 0.0 3 0 0 0 39 21 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 33 3 0.0 3 1 0 0 2 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 1 2 56 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 10,622 Reads with adapters: 135 (1.3%) Reads written (passing filters): 10,622 (100.0%) Total basepairs processed: 2,926,873 bp Total written (filtered): 2,926,466 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 135 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 80.7% C: 4.4% G: 12.6% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 133 166.0 0 133 4 2 41.5 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.