This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 79.96 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 316,845 (10.5%) Reads that were too short: 37,437 (1.2%) Reads written (passing filters): 279,408 (9.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 74,820,337 bp (8.3%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 316845 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1628 734.8 0 1628 7 218457 734.8 0 218457 8 10826 734.8 0 10826 9 31 734.8 0 31 10 17 734.8 0 17 11 12 734.8 0 12 12 43 734.8 0 43 13 90 734.8 0 90 14 107 734.8 0 107 15 52 734.8 0 52 16 14 734.8 0 14 17 4 734.8 0 4 18 6 734.8 0 6 19 8 734.8 0 8 20 5 734.8 0 5 21 10 734.8 0 10 22 4 734.8 0 4 23 7 734.8 0 7 24 16 734.8 0 16 25 621 734.8 0 621 26 76 734.8 0 76 27 7 734.8 0 7 28 4 734.8 0 4 29 3 734.8 0 3 30 7 734.8 0 7 31 5 734.8 0 5 32 9 734.8 0 9 33 10 734.8 0 10 34 8 734.8 0 8 35 7 734.8 0 7 36 8 734.8 0 8 37 6 734.8 0 6 38 4 734.8 0 4 39 7 734.8 0 7 40 7 734.8 0 7 41 9 734.8 0 9 42 35 734.8 0 35 43 15 734.8 0 15 44 8 734.8 0 8 45 12 734.8 0 12 46 12 734.8 0 12 47 7 734.8 0 7 48 13 734.8 0 13 49 12 734.8 0 12 50 14 734.8 0 14 51 5 734.8 0 5 52 3 734.8 0 3 53 17 734.8 0 17 54 8 734.8 0 8 55 11 734.8 0 11 56 24 734.8 0 24 57 13 734.8 0 13 58 12 734.8 0 12 59 10 734.8 0 10 60 25 734.8 0 25 61 31 734.8 0 31 62 49 734.8 0 49 63 15 734.8 0 15 64 5 734.8 0 5 65 9 734.8 0 9 66 10 734.8 0 10 67 10 734.8 0 10 68 9 734.8 0 9 69 10 734.8 0 10 70 13 734.8 0 13 71 78 734.8 0 78 72 16 734.8 0 16 73 9 734.8 0 9 74 21 734.8 0 21 75 6 734.8 0 6 76 13 734.8 0 13 77 15 734.8 0 15 78 14 734.8 0 14 79 26 734.8 0 26 80 45 734.8 0 45 81 101 734.8 0 101 82 372 734.8 0 372 83 343 734.8 0 343 84 146 734.8 0 146 85 108 734.8 0 108 86 68 734.8 0 68 87 80 734.8 0 80 88 379 734.8 0 379 89 238 734.8 0 238 90 188 734.8 0 188 91 95 734.8 0 95 92 58 734.8 0 58 93 130 734.8 0 130 94 155 734.8 0 155 95 106 734.8 0 106 96 137 734.8 0 137 97 105 734.8 0 105 98 156 734.8 0 156 99 246 734.8 0 246 100 111 734.8 0 111 101 94 734.8 0 94 102 224 734.8 0 224 103 383 734.8 0 383 104 173 734.8 0 173 105 123 734.8 0 123 106 131 734.8 0 131 107 167 734.8 0 167 108 62 734.8 0 62 109 38 734.8 0 38 110 118 734.8 0 118 111 264 734.8 0 264 112 326 734.8 0 326 113 170 734.8 0 170 114 209 734.8 0 209 115 143 734.8 0 143 116 87 734.8 0 87 117 83 734.8 0 83 118 234 734.8 0 234 119 312 734.8 0 312 120 354 734.8 0 354 121 205 734.8 0 205 122 205 734.8 0 205 123 287 734.8 0 287 124 247 734.8 0 247 125 154 734.8 0 154 126 226 734.8 0 226 127 198 734.8 0 198 128 160 734.8 0 160 129 203 734.8 0 203 130 183 734.8 0 183 131 154 734.8 0 154 132 199 734.8 0 199 133 213 734.8 0 213 134 258 734.8 0 258 135 200 734.8 0 200 136 153 734.8 0 153 137 192 734.8 0 192 138 225 734.8 0 225 139 443 734.8 0 443 140 538 734.8 0 538 141 374 734.8 0 374 142 203 734.8 0 203 143 207 734.8 0 207 144 290 734.8 0 290 145 241 734.8 0 241 146 235 734.8 0 235 147 250 734.8 0 250 148 251 734.8 0 251 149 183 734.8 0 183 150 184 734.8 0 184 151 192 734.8 0 192 152 264 734.8 0 264 153 330 734.8 0 330 154 242 734.8 0 242 155 209 734.8 0 209 156 201 734.8 0 201 157 227 734.8 0 227 158 277 734.8 0 277 159 339 734.8 0 339 160 868 734.8 0 868 161 898 734.8 0 898 162 511 734.8 0 511 163 243 734.8 0 243 164 221 734.8 0 221 165 205 734.8 0 205 166 163 734.8 0 163 167 195 734.8 0 195 168 295 734.8 0 295 169 304 734.8 0 304 170 243 734.8 0 243 171 179 734.8 0 179 172 244 734.8 0 244 173 328 734.8 0 328 174 211 734.8 0 211 175 217 734.8 0 217 176 240 734.8 0 240 177 285 734.8 0 285 178 241 734.8 0 241 179 278 734.8 0 278 180 367 734.8 0 367 181 323 734.8 0 323 182 461 734.8 0 461 183 325 734.8 0 325 184 366 734.8 0 366 185 460 734.8 0 460 186 538 734.8 0 538 187 6223 734.8 0 6223 188 10037 734.8 0 10037 189 1711 734.8 0 1711 190 326 734.8 0 326 191 214 734.8 0 214 192 177 734.8 0 177 193 230 734.8 0 230 194 175 734.8 0 175 195 196 734.8 0 196 196 316 734.8 0 316 197 315 734.8 0 315 198 232 734.8 0 232 199 205 734.8 0 205 200 248 734.8 0 248 201 440 734.8 0 440 202 582 734.8 0 582 203 275 734.8 0 275 204 249 734.8 0 249 205 288 734.8 0 288 206 437 734.8 0 437 207 394 734.8 0 394 208 364 734.8 0 364 209 274 734.8 0 274 210 313 734.8 0 313 211 358 734.8 0 358 212 296 734.8 0 296 213 288 734.8 0 288 214 293 734.8 0 293 215 282 734.8 0 282 216 277 734.8 0 277 217 232 734.8 0 232 218 306 734.8 0 306 219 431 734.8 0 431 220 353 734.8 0 353 221 388 734.8 0 388 222 396 734.8 0 396 223 290 734.8 0 290 224 245 734.8 0 245 225 260 734.8 0 260 226 297 734.8 0 297 227 290 734.8 0 290 228 263 734.8 0 263 229 364 734.8 0 364 230 527 734.8 0 527 231 471 734.8 0 471 232 336 734.8 0 336 233 320 734.8 0 320 234 341 734.8 0 341 235 310 734.8 0 310 236 169 734.8 0 169 237 254 734.8 0 254 238 340 734.8 0 340 239 417 734.8 0 417 240 575 734.8 0 575 241 484 734.8 0 484 242 456 734.8 0 456 243 279 734.8 0 279 244 281 734.8 0 281 245 282 734.8 0 282 246 319 734.8 0 319 247 181 734.8 0 181 248 143 734.8 0 143 249 141 734.8 0 141 250 381 734.8 0 381 251 469 734.8 0 469 252 307 734.8 0 307 253 227 734.8 0 227 254 268 734.8 0 268 255 275 734.8 0 275 256 308 734.8 0 308 257 281 734.8 0 281 258 299 734.8 0 299 259 225 734.8 0 225 260 260 734.8 0 260 261 277 734.8 0 277 262 381 734.8 0 381 263 288 734.8 0 288 264 339 734.8 0 339 265 255 734.8 0 255 266 353 734.8 0 353 267 246 734.8 0 246 268 216 734.8 0 216 269 245 734.8 0 245 270 457 734.8 0 457 271 364 734.8 0 364 272 291 734.8 0 291 273 291 734.8 0 291 274 361 734.8 0 361 275 563 734.8 0 563 276 469 734.8 0 469 277 431 734.8 0 431 278 473 734.8 0 473 279 466 734.8 0 466 280 258 734.8 0 258 281 274 734.8 0 274 282 429 734.8 0 429 283 554 734.8 0 554 284 356 734.8 0 356 285 371 734.8 0 371 286 290 734.8 0 290 287 245 734.8 0 245 288 204 734.8 0 204 289 403 734.8 0 403 290 450 734.8 0 450 291 333 734.8 0 333 292 401 734.8 0 401 293 317 734.8 0 317 294 299 734.8 0 299 295 382 734.8 0 382 296 423 734.8 0 423 297 333 734.8 0 333 298 394 734.8 0 394 299 471 734.8 0 471 300 545 734.8 0 545 301 3928 734.8 0 3928 Finished in 13.75 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 279,408 Reads with adapters: 14,019 (5.0%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 13,999 (5.0%) Total basepairs processed: 74,820,337 bp Total written (filtered): 3,487,937 bp (4.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14019 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 10 0.0 3 0 0 9 1 19 119 0.0 3 0 117 0 0 2 20 10685 0.0 4 10565 117 3 21 27 0.0 4 8 18 1 22 3 0.0 4 0 0 3 23 4 0.0 4 0 0 0 2 2 25 1 0.0 4 1 33 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 6 0.0 4 0 0 0 0 6 126 33 0.0 4 0 0 0 0 33 127 3093 0.0 4 0 0 0 0 3093 128 8 0.0 4 0 0 0 0 8 131 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 201 1 0.0 4 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 1 287 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.46 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 13,999 Reads with adapters: 691 (4.9%) Reads written (passing filters): 13,999 (100.0%) Total basepairs processed: 3,487,937 bp Total written (filtered): 3,468,342 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 691 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.5% C: 1.0% G: 91.3% T: 2.0% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 218.7 0 4 10 39 0.0 1 2 37 11 600 0.0 1 0 600 258 2 0.0 1 2 259 29 0.0 1 29 260 3 0.0 1 3 270 10 0.0 1 10 271 3 0.0 1 3 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.