This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.32 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 490,060 (16.3%) Reads that were too short: 218,723 (7.3%) Reads written (passing filters): 271,337 (9.0%) Total basepairs processed: 905,907,359 bp Total written (filtered): 50,531,667 bp (5.6%) === Adapter 1 === Sequence: AACAAC; Type: regular 5'; Length: 6; Trimmed: 490060 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 436 734.8 0 436 7 36978 734.8 0 36978 8 455 734.8 0 455 9 43 734.8 0 43 10 41 734.8 0 41 11 49 734.8 0 49 12 41 734.8 0 41 13 30 734.8 0 30 14 42 734.8 0 42 15 53 734.8 0 53 16 42 734.8 0 42 17 30 734.8 0 30 18 42 734.8 0 42 19 34 734.8 0 34 20 28 734.8 0 28 21 22 734.8 0 22 22 40 734.8 0 40 23 37 734.8 0 37 24 30 734.8 0 30 25 40 734.8 0 40 26 50 734.8 0 50 27 33 734.8 0 33 28 27 734.8 0 27 29 41 734.8 0 41 30 22 734.8 0 22 31 36 734.8 0 36 32 30 734.8 0 30 33 23 734.8 0 23 34 35 734.8 0 35 35 37 734.8 0 37 36 33 734.8 0 33 37 35 734.8 0 35 38 27 734.8 0 27 39 37 734.8 0 37 40 34 734.8 0 34 41 27 734.8 0 27 42 41 734.8 0 41 43 35 734.8 0 35 44 32 734.8 0 32 45 24 734.8 0 24 46 42 734.8 0 42 47 31 734.8 0 31 48 26 734.8 0 26 49 28 734.8 0 28 50 31 734.8 0 31 51 36 734.8 0 36 52 30 734.8 0 30 53 39 734.8 0 39 54 45 734.8 0 45 55 37 734.8 0 37 56 32 734.8 0 32 57 35 734.8 0 35 58 44 734.8 0 44 59 38 734.8 0 38 60 66 734.8 0 66 61 56 734.8 0 56 62 85 734.8 0 85 63 159 734.8 0 159 64 113 734.8 0 113 65 484 734.8 0 484 66 396 734.8 0 396 67 397 734.8 0 397 68 201 734.8 0 201 69 262 734.8 0 262 70 1079 734.8 0 1079 71 4307 734.8 0 4307 72 3248 734.8 0 3248 73 2596 734.8 0 2596 74 2604 734.8 0 2604 75 1168 734.8 0 1168 76 1412 734.8 0 1412 77 1506 734.8 0 1506 78 2065 734.8 0 2065 79 1821 734.8 0 1821 80 1096 734.8 0 1096 81 1807 734.8 0 1807 82 1180 734.8 0 1180 83 1363 734.8 0 1363 84 1273 734.8 0 1273 85 1062 734.8 0 1062 86 960 734.8 0 960 87 1608 734.8 0 1608 88 1498 734.8 0 1498 89 1369 734.8 0 1369 90 2201 734.8 0 2201 91 1934 734.8 0 1934 92 1408 734.8 0 1408 93 1356 734.8 0 1356 94 862 734.8 0 862 95 655 734.8 0 655 96 714 734.8 0 714 97 646 734.8 0 646 98 658 734.8 0 658 99 750 734.8 0 750 100 1374 734.8 0 1374 101 1821 734.8 0 1821 102 1840 734.8 0 1840 103 2936 734.8 0 2936 104 4116 734.8 0 4116 105 4008 734.8 0 4008 106 1984 734.8 0 1984 107 2638 734.8 0 2638 108 2609 734.8 0 2609 109 2116 734.8 0 2116 110 2579 734.8 0 2579 111 2287 734.8 0 2287 112 1850 734.8 0 1850 113 1599 734.8 0 1599 114 1743 734.8 0 1743 115 1760 734.8 0 1760 116 1624 734.8 0 1624 117 1605 734.8 0 1605 118 1752 734.8 0 1752 119 1685 734.8 0 1685 120 2214 734.8 0 2214 121 2329 734.8 0 2329 122 1964 734.8 0 1964 123 2512 734.8 0 2512 124 2554 734.8 0 2554 125 2488 734.8 0 2488 126 2054 734.8 0 2054 127 1562 734.8 0 1562 128 1626 734.8 0 1626 129 1526 734.8 0 1526 130 1415 734.8 0 1415 131 1544 734.8 0 1544 132 1760 734.8 0 1760 133 1651 734.8 0 1651 134 1662 734.8 0 1662 135 1360 734.8 0 1360 136 1463 734.8 0 1463 137 1432 734.8 0 1432 138 1436 734.8 0 1436 139 1287 734.8 0 1287 140 1608 734.8 0 1608 141 1630 734.8 0 1630 142 1732 734.8 0 1732 143 1792 734.8 0 1792 144 1622 734.8 0 1622 145 1860 734.8 0 1860 146 1909 734.8 0 1909 147 1856 734.8 0 1856 148 1618 734.8 0 1618 149 1992 734.8 0 1992 150 2063 734.8 0 2063 151 1856 734.8 0 1856 152 2042 734.8 0 2042 153 1978 734.8 0 1978 154 1797 734.8 0 1797 155 1517 734.8 0 1517 156 1476 734.8 0 1476 157 1624 734.8 0 1624 158 1500 734.8 0 1500 159 1589 734.8 0 1589 160 1609 734.8 0 1609 161 2049 734.8 0 2049 162 2270 734.8 0 2270 163 2144 734.8 0 2144 164 1494 734.8 0 1494 165 1366 734.8 0 1366 166 1526 734.8 0 1526 167 1372 734.8 0 1372 168 1565 734.8 0 1565 169 1802 734.8 0 1802 170 2040 734.8 0 2040 171 2304 734.8 0 2304 172 1977 734.8 0 1977 173 1610 734.8 0 1610 174 1930 734.8 0 1930 175 1558 734.8 0 1558 176 1283 734.8 0 1283 177 1696 734.8 0 1696 178 1765 734.8 0 1765 179 1681 734.8 0 1681 180 1562 734.8 0 1562 181 1934 734.8 0 1934 182 1968 734.8 0 1968 183 1720 734.8 0 1720 184 1241 734.8 0 1241 185 1187 734.8 0 1187 186 1355 734.8 0 1355 187 1648 734.8 0 1648 188 1570 734.8 0 1570 189 1570 734.8 0 1570 190 1781 734.8 0 1781 191 1598 734.8 0 1598 192 1457 734.8 0 1457 193 1141 734.8 0 1141 194 1626 734.8 0 1626 195 1628 734.8 0 1628 196 1046 734.8 0 1046 197 1454 734.8 0 1454 198 1533 734.8 0 1533 199 1608 734.8 0 1608 200 1572 734.8 0 1572 201 1180 734.8 0 1180 202 1308 734.8 0 1308 203 1633 734.8 0 1633 204 1550 734.8 0 1550 205 1177 734.8 0 1177 206 1007 734.8 0 1007 207 1079 734.8 0 1079 208 1181 734.8 0 1181 209 1038 734.8 0 1038 210 979 734.8 0 979 211 1129 734.8 0 1129 212 1203 734.8 0 1203 213 1633 734.8 0 1633 214 1710 734.8 0 1710 215 1594 734.8 0 1594 216 1482 734.8 0 1482 217 1544 734.8 0 1544 218 1779 734.8 0 1779 219 1406 734.8 0 1406 220 1729 734.8 0 1729 221 1240 734.8 0 1240 222 1193 734.8 0 1193 223 1459 734.8 0 1459 224 1819 734.8 0 1819 225 2206 734.8 0 2206 226 4747 734.8 0 4747 227 4394 734.8 0 4394 228 2622 734.8 0 2622 229 2956 734.8 0 2956 230 7337 734.8 0 7337 231 6117 734.8 0 6117 232 2233 734.8 0 2233 233 1458 734.8 0 1458 234 1312 734.8 0 1312 235 1292 734.8 0 1292 236 1414 734.8 0 1414 237 1170 734.8 0 1170 238 1131 734.8 0 1131 239 1892 734.8 0 1892 240 1737 734.8 0 1737 241 1325 734.8 0 1325 242 1156 734.8 0 1156 243 1488 734.8 0 1488 244 1362 734.8 0 1362 245 979 734.8 0 979 246 1096 734.8 0 1096 247 1335 734.8 0 1335 248 1431 734.8 0 1431 249 1933 734.8 0 1933 250 1806 734.8 0 1806 251 1830 734.8 0 1830 252 2134 734.8 0 2134 253 1951 734.8 0 1951 254 1376 734.8 0 1376 255 991 734.8 0 991 256 883 734.8 0 883 257 1017 734.8 0 1017 258 1188 734.8 0 1188 259 1341 734.8 0 1341 260 1594 734.8 0 1594 261 1904 734.8 0 1904 262 2881 734.8 0 2881 263 8351 734.8 0 8351 264 14581 734.8 0 14581 265 17932 734.8 0 17932 266 3973 734.8 0 3973 267 1660 734.8 0 1660 268 2095 734.8 0 2095 269 2264 734.8 0 2264 270 1340 734.8 0 1340 271 1685 734.8 0 1685 272 1874 734.8 0 1874 273 1402 734.8 0 1402 274 1785 734.8 0 1785 275 2532 734.8 0 2532 276 1808 734.8 0 1808 277 2110 734.8 0 2110 278 2135 734.8 0 2135 279 2070 734.8 0 2070 280 1971 734.8 0 1971 281 1685 734.8 0 1685 282 1359 734.8 0 1359 283 2022 734.8 0 2022 284 1973 734.8 0 1973 285 1418 734.8 0 1418 286 1645 734.8 0 1645 287 1935 734.8 0 1935 288 1707 734.8 0 1707 289 2049 734.8 0 2049 290 3316 734.8 0 3316 291 3113 734.8 0 3113 292 2031 734.8 0 2031 293 1543 734.8 0 1543 294 1288 734.8 0 1288 295 1279 734.8 0 1279 296 1569 734.8 0 1569 297 1575 734.8 0 1575 298 1548 734.8 0 1548 299 1576 734.8 0 1576 300 2327 734.8 0 2327 301 1306 734.8 0 1306 Finished in 9.12 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 271,337 Reads with adapters: 37,461 (13.8%) Reads that were too short: 107 (0.0%) Reads written (passing filters): 37,354 (13.8%) Total basepairs processed: 50,531,667 bp Total written (filtered): 10,316,866 bp (20.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 37461 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 4 2 16 268 0.0 3 0 246 12 10 17 36692 0.0 3 35714 810 127 41 18 102 0.0 3 6 81 9 6 19 17 0.0 3 1 0 11 5 20 79 0.0 3 31 1 1 46 21 4 0.0 3 0 1 0 3 22 2 0.0 3 0 0 0 2 23 8 0.0 3 0 0 0 8 24 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 26 4 0.0 3 1 0 0 3 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 33 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 36 3 0.0 3 0 0 0 3 37 3 0.0 3 0 0 0 3 38 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 49 2 0.0 3 0 0 0 2 50 2 0.0 3 0 0 0 2 51 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 44 0.0 3 0 0 1 43 69 2 0.0 3 0 0 0 2 70 3 0.0 3 0 0 0 3 71 17 0.0 3 0 0 0 17 72 2 0.0 3 0 0 1 1 73 3 0.0 3 0 0 0 3 74 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 3 0.0 3 0 0 0 3 79 2 0.0 3 0 0 0 2 80 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 1 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 3 0.0 3 0 0 0 3 92 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 5 0.0 3 0 0 0 5 115 2 0.0 3 0 0 0 2 116 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 122 5 0.0 3 0 0 1 4 123 7 0.0 3 0 0 0 7 124 5 0.0 3 0 0 0 5 125 6 0.0 3 0 0 0 6 126 14 0.0 3 0 0 0 14 127 2 0.0 3 0 0 0 2 128 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 133 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 136 3 0.0 3 0 0 0 3 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 2 0.0 3 0 0 0 2 169 3 0.0 3 0 0 0 3 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 7 0.0 3 0 0 0 7 184 1 0.0 3 0 0 0 1 186 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.32 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 37,354 Reads with adapters: 879 (2.4%) Reads written (passing filters): 37,354 (100.0%) Total basepairs processed: 10,316,866 bp Total written (filtered): 10,314,215 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 879 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.2% C: 49.3% G: 14.3% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 869 583.7 0 869 4 6 145.9 0 6 5 4 36.5 0 4