This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.53 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 97,617 (3.2%) Reads that were too short: 24,056 (0.8%) Reads written (passing filters): 73,561 (2.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 17,085,554 bp (1.9%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 97617 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1294 734.8 0 1294 7 34713 734.8 0 34713 8 2373 734.8 0 2373 9 3258 734.8 0 3258 10 13 734.8 0 13 11 14 734.8 0 14 12 15 734.8 0 15 13 20 734.8 0 20 14 16 734.8 0 16 15 15 734.8 0 15 16 12 734.8 0 12 17 17 734.8 0 17 18 12 734.8 0 12 19 25 734.8 0 25 20 12 734.8 0 12 21 25 734.8 0 25 22 18 734.8 0 18 23 18 734.8 0 18 24 11 734.8 0 11 25 18 734.8 0 18 26 9 734.8 0 9 27 12 734.8 0 12 28 28 734.8 0 28 29 20 734.8 0 20 30 14 734.8 0 14 31 22 734.8 0 22 32 11 734.8 0 11 33 18 734.8 0 18 34 52 734.8 0 52 35 21 734.8 0 21 36 12 734.8 0 12 37 21 734.8 0 21 38 45 734.8 0 45 39 49 734.8 0 49 40 12 734.8 0 12 41 27 734.8 0 27 42 88 734.8 0 88 43 116 734.8 0 116 44 28 734.8 0 28 45 12 734.8 0 12 46 16 734.8 0 16 47 17 734.8 0 17 48 9 734.8 0 9 49 13 734.8 0 13 50 23 734.8 0 23 51 21 734.8 0 21 52 24 734.8 0 24 53 118 734.8 0 118 54 77 734.8 0 77 55 28 734.8 0 28 56 12 734.8 0 12 57 32 734.8 0 32 58 13 734.8 0 13 59 9 734.8 0 9 60 16 734.8 0 16 61 21 734.8 0 21 62 19 734.8 0 19 63 19 734.8 0 19 64 11 734.8 0 11 65 24 734.8 0 24 66 16 734.8 0 16 67 21 734.8 0 21 68 10 734.8 0 10 69 19 734.8 0 19 70 25 734.8 0 25 71 76 734.8 0 76 72 19 734.8 0 19 73 21 734.8 0 21 74 11 734.8 0 11 75 11 734.8 0 11 76 9 734.8 0 9 77 19 734.8 0 19 78 19 734.8 0 19 79 19 734.8 0 19 80 17 734.8 0 17 81 34 734.8 0 34 82 37 734.8 0 37 83 34 734.8 0 34 84 36 734.8 0 36 85 22 734.8 0 22 86 20 734.8 0 20 87 39 734.8 0 39 88 35 734.8 0 35 89 34 734.8 0 34 90 23 734.8 0 23 91 77 734.8 0 77 92 51 734.8 0 51 93 105 734.8 0 105 94 108 734.8 0 108 95 73 734.8 0 73 96 34 734.8 0 34 97 30 734.8 0 30 98 49 734.8 0 49 99 42 734.8 0 42 100 31 734.8 0 31 101 57 734.8 0 57 102 78 734.8 0 78 103 105 734.8 0 105 104 119 734.8 0 119 105 69 734.8 0 69 106 50 734.8 0 50 107 128 734.8 0 128 108 39 734.8 0 39 109 40 734.8 0 40 110 48 734.8 0 48 111 167 734.8 0 167 112 179 734.8 0 179 113 216 734.8 0 216 114 174 734.8 0 174 115 107 734.8 0 107 116 52 734.8 0 52 117 44 734.8 0 44 118 47 734.8 0 47 119 53 734.8 0 53 120 79 734.8 0 79 121 51 734.8 0 51 122 64 734.8 0 64 123 92 734.8 0 92 124 149 734.8 0 149 125 651 734.8 0 651 126 806 734.8 0 806 127 422 734.8 0 422 128 62 734.8 0 62 129 100 734.8 0 100 130 136 734.8 0 136 131 78 734.8 0 78 132 118 734.8 0 118 133 146 734.8 0 146 134 173 734.8 0 173 135 77 734.8 0 77 136 148 734.8 0 148 137 102 734.8 0 102 138 135 734.8 0 135 139 144 734.8 0 144 140 126 734.8 0 126 141 95 734.8 0 95 142 85 734.8 0 85 143 117 734.8 0 117 144 270 734.8 0 270 145 270 734.8 0 270 146 271 734.8 0 271 147 156 734.8 0 156 148 115 734.8 0 115 149 154 734.8 0 154 150 276 734.8 0 276 151 616 734.8 0 616 152 278 734.8 0 278 153 185 734.8 0 185 154 190 734.8 0 190 155 672 734.8 0 672 156 301 734.8 0 301 157 330 734.8 0 330 158 366 734.8 0 366 159 522 734.8 0 522 160 4270 734.8 0 4270 161 5145 734.8 0 5145 162 1777 734.8 0 1777 163 149 734.8 0 149 164 303 734.8 0 303 165 278 734.8 0 278 166 173 734.8 0 173 167 105 734.8 0 105 168 100 734.8 0 100 169 164 734.8 0 164 170 148 734.8 0 148 171 122 734.8 0 122 172 132 734.8 0 132 173 116 734.8 0 116 174 117 734.8 0 117 175 138 734.8 0 138 176 128 734.8 0 128 177 199 734.8 0 199 178 152 734.8 0 152 179 222 734.8 0 222 180 589 734.8 0 589 181 620 734.8 0 620 182 590 734.8 0 590 183 186 734.8 0 186 184 138 734.8 0 138 185 137 734.8 0 137 186 332 734.8 0 332 187 182 734.8 0 182 188 129 734.8 0 129 189 166 734.8 0 166 190 211 734.8 0 211 191 178 734.8 0 178 192 94 734.8 0 94 193 67 734.8 0 67 194 74 734.8 0 74 195 78 734.8 0 78 196 114 734.8 0 114 197 99 734.8 0 99 198 87 734.8 0 87 199 111 734.8 0 111 200 103 734.8 0 103 201 174 734.8 0 174 202 98 734.8 0 98 203 84 734.8 0 84 204 91 734.8 0 91 205 190 734.8 0 190 206 115 734.8 0 115 207 97 734.8 0 97 208 113 734.8 0 113 209 112 734.8 0 112 210 132 734.8 0 132 211 184 734.8 0 184 212 824 734.8 0 824 213 1068 734.8 0 1068 214 333 734.8 0 333 215 145 734.8 0 145 216 245 734.8 0 245 217 235 734.8 0 235 218 242 734.8 0 242 219 145 734.8 0 145 220 134 734.8 0 134 221 208 734.8 0 208 222 184 734.8 0 184 223 153 734.8 0 153 224 164 734.8 0 164 225 305 734.8 0 305 226 194 734.8 0 194 227 216 734.8 0 216 228 159 734.8 0 159 229 222 734.8 0 222 230 172 734.8 0 172 231 144 734.8 0 144 232 110 734.8 0 110 233 146 734.8 0 146 234 141 734.8 0 141 235 125 734.8 0 125 236 155 734.8 0 155 237 238 734.8 0 238 238 167 734.8 0 167 239 343 734.8 0 343 240 287 734.8 0 287 241 303 734.8 0 303 242 193 734.8 0 193 243 321 734.8 0 321 244 234 734.8 0 234 245 340 734.8 0 340 246 258 734.8 0 258 247 225 734.8 0 225 248 151 734.8 0 151 249 133 734.8 0 133 250 229 734.8 0 229 251 131 734.8 0 131 252 105 734.8 0 105 253 132 734.8 0 132 254 302 734.8 0 302 255 310 734.8 0 310 256 498 734.8 0 498 257 496 734.8 0 496 258 284 734.8 0 284 259 156 734.8 0 156 260 251 734.8 0 251 261 213 734.8 0 213 262 202 734.8 0 202 263 182 734.8 0 182 264 237 734.8 0 237 265 210 734.8 0 210 266 616 734.8 0 616 267 444 734.8 0 444 268 290 734.8 0 290 269 206 734.8 0 206 270 265 734.8 0 265 271 293 734.8 0 293 272 378 734.8 0 378 273 906 734.8 0 906 274 592 734.8 0 592 275 311 734.8 0 311 276 299 734.8 0 299 277 334 734.8 0 334 278 282 734.8 0 282 279 204 734.8 0 204 280 206 734.8 0 206 281 315 734.8 0 315 282 470 734.8 0 470 283 218 734.8 0 218 284 218 734.8 0 218 285 249 734.8 0 249 286 163 734.8 0 163 287 300 734.8 0 300 288 291 734.8 0 291 289 424 734.8 0 424 290 183 734.8 0 183 291 106 734.8 0 106 292 86 734.8 0 86 293 84 734.8 0 84 294 65 734.8 0 65 295 83 734.8 0 83 296 63 734.8 0 63 297 83 734.8 0 83 298 144 734.8 0 144 299 105 734.8 0 105 300 132 734.8 0 132 301 132 734.8 0 132 Finished in 3.19 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 73,561 Reads with adapters: 35,925 (48.8%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 35,914 (48.8%) Total basepairs processed: 17,085,554 bp Total written (filtered): 9,836,365 bp (57.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35925 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 26 0.0 3 0 0 23 2 1 19 586 0.0 3 0 578 6 2 20 34871 0.0 4 34394 461 9 4 3 21 348 0.0 4 288 59 1 22 9 0.0 4 1 2 6 23 17 0.0 4 2 0 1 12 2 24 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 0 2 73 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 111 30 0.0 4 0 0 1 22 7 112 3 0.0 4 0 0 0 3 127 15 0.0 4 0 0 0 0 15 128 4 0.0 4 0 0 0 0 4 131 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.20 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 35,914 Reads with adapters: 433 (1.2%) Reads written (passing filters): 35,914 (100.0%) Total basepairs processed: 9,836,365 bp Total written (filtered): 9,820,645 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 433 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.2% C: 1.8% G: 85.2% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 561.2 0 6 11 374 0.0 1 1 373 12 7 0.0 1 0 7 20 1 0.0 1 1 191 1 0.0 1 1 210 3 0.0 1 0 3 230 1 0.0 1 1 237 1 0.0 1 1 259 1 0.0 1 1 260 2 0.0 1 2 261 17 0.0 1 17 262 17 0.0 1 16 1 263 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.