This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.59 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 131,665 (5.0%) Reads that were too short: 14,571 (0.6%) Reads written (passing filters): 117,094 (4.5%) Total basepairs processed: 791,719,999 bp Total written (filtered): 20,004,331 bp (2.5%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 131665 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 140 642.2 0 140 7 887 642.2 0 887 8 850 642.2 0 850 9 147 642.2 0 147 10 106 642.2 0 106 11 66 642.2 0 66 12 89 642.2 0 89 13 73 642.2 0 73 14 135 642.2 0 135 15 109 642.2 0 109 16 79 642.2 0 79 17 138 642.2 0 138 18 113 642.2 0 113 19 81 642.2 0 81 20 98 642.2 0 98 21 103 642.2 0 103 22 101 642.2 0 101 23 88 642.2 0 88 24 81 642.2 0 81 25 89 642.2 0 89 26 113 642.2 0 113 27 217 642.2 0 217 28 377 642.2 0 377 29 333 642.2 0 333 30 94 642.2 0 94 31 89 642.2 0 89 32 117 642.2 0 117 33 117 642.2 0 117 34 125 642.2 0 125 35 521 642.2 0 521 36 506 642.2 0 506 37 101 642.2 0 101 38 107 642.2 0 107 39 94 642.2 0 94 40 74 642.2 0 74 41 78 642.2 0 78 42 96 642.2 0 96 43 81 642.2 0 81 44 92 642.2 0 92 45 134 642.2 0 134 46 149 642.2 0 149 47 89 642.2 0 89 48 102 642.2 0 102 49 83 642.2 0 83 50 133 642.2 0 133 51 168 642.2 0 168 52 149 642.2 0 149 53 92 642.2 0 92 54 113 642.2 0 113 55 100 642.2 0 100 56 109 642.2 0 109 57 132 642.2 0 132 58 441 642.2 0 441 59 479 642.2 0 479 60 413 642.2 0 413 61 90 642.2 0 90 62 107 642.2 0 107 63 102 642.2 0 102 64 78 642.2 0 78 65 85 642.2 0 85 66 98 642.2 0 98 67 78 642.2 0 78 68 117 642.2 0 117 69 225 642.2 0 225 70 357 642.2 0 357 71 237 642.2 0 237 72 1568 642.2 0 1568 73 1079 642.2 0 1079 74 1293 642.2 0 1293 75 83 642.2 0 83 76 86 642.2 0 86 77 103 642.2 0 103 78 86 642.2 0 86 79 77 642.2 0 77 80 63 642.2 0 63 81 74 642.2 0 74 82 63 642.2 0 63 83 102 642.2 0 102 84 93 642.2 0 93 85 85 642.2 0 85 86 88 642.2 0 88 87 93 642.2 0 93 88 146 642.2 0 146 89 128 642.2 0 128 90 289 642.2 0 289 91 159 642.2 0 159 92 380 642.2 0 380 93 97 642.2 0 97 94 68 642.2 0 68 95 104 642.2 0 104 96 96 642.2 0 96 97 89 642.2 0 89 98 136 642.2 0 136 99 87 642.2 0 87 100 74 642.2 0 74 101 101 642.2 0 101 102 95 642.2 0 95 103 142 642.2 0 142 104 110 642.2 0 110 105 64 642.2 0 64 106 78 642.2 0 78 107 110 642.2 0 110 108 119 642.2 0 119 109 124 642.2 0 124 110 140 642.2 0 140 111 81 642.2 0 81 112 184 642.2 0 184 113 354 642.2 0 354 114 564 642.2 0 564 115 74 642.2 0 74 116 89 642.2 0 89 117 128 642.2 0 128 118 85 642.2 0 85 119 178 642.2 0 178 120 87 642.2 0 87 121 140 642.2 0 140 122 83 642.2 0 83 123 97 642.2 0 97 124 80 642.2 0 80 125 85 642.2 0 85 126 95 642.2 0 95 127 98 642.2 0 98 128 111 642.2 0 111 129 234 642.2 0 234 130 357 642.2 0 357 131 138 642.2 0 138 132 142 642.2 0 142 133 205 642.2 0 205 134 148 642.2 0 148 135 160 642.2 0 160 136 247 642.2 0 247 137 216 642.2 0 216 138 232 642.2 0 232 139 467 642.2 0 467 140 494 642.2 0 494 141 652 642.2 0 652 142 1111 642.2 0 1111 143 10223 642.2 0 10223 144 67829 642.2 0 67829 145 4795 642.2 0 4795 146 141 642.2 0 141 147 113 642.2 0 113 148 171 642.2 0 171 149 455 642.2 0 455 150 116 642.2 0 116 151 65 642.2 0 65 152 111 642.2 0 111 153 64 642.2 0 64 154 85 642.2 0 85 155 261 642.2 0 261 156 276 642.2 0 276 157 78 642.2 0 78 158 105 642.2 0 105 159 95 642.2 0 95 160 96 642.2 0 96 161 96 642.2 0 96 162 139 642.2 0 139 163 1400 642.2 0 1400 164 184 642.2 0 184 165 125 642.2 0 125 166 76 642.2 0 76 167 95 642.2 0 95 168 108 642.2 0 108 169 82 642.2 0 82 170 87 642.2 0 87 171 102 642.2 0 102 172 77 642.2 0 77 173 100 642.2 0 100 174 91 642.2 0 91 175 64 642.2 0 64 176 105 642.2 0 105 177 86 642.2 0 86 178 86 642.2 0 86 179 89 642.2 0 89 180 82 642.2 0 82 181 71 642.2 0 71 182 86 642.2 0 86 183 101 642.2 0 101 184 95 642.2 0 95 185 100 642.2 0 100 186 92 642.2 0 92 187 109 642.2 0 109 188 143 642.2 0 143 189 90 642.2 0 90 190 78 642.2 0 78 191 75 642.2 0 75 192 82 642.2 0 82 193 82 642.2 0 82 194 95 642.2 0 95 195 89 642.2 0 89 196 126 642.2 0 126 197 90 642.2 0 90 198 124 642.2 0 124 199 75 642.2 0 75 200 98 642.2 0 98 201 87 642.2 0 87 202 74 642.2 0 74 203 118 642.2 0 118 204 105 642.2 0 105 205 102 642.2 0 102 206 114 642.2 0 114 207 103 642.2 0 103 208 133 642.2 0 133 209 145 642.2 0 145 210 152 642.2 0 152 211 181 642.2 0 181 212 135 642.2 0 135 213 230 642.2 0 230 214 196 642.2 0 196 215 865 642.2 0 865 216 670 642.2 0 670 217 722 642.2 0 722 218 89 642.2 0 89 219 77 642.2 0 77 220 94 642.2 0 94 221 129 642.2 0 129 222 130 642.2 0 130 223 118 642.2 0 118 224 138 642.2 0 138 225 152 642.2 0 152 226 126 642.2 0 126 227 105 642.2 0 105 228 157 642.2 0 157 229 85 642.2 0 85 230 104 642.2 0 104 231 206 642.2 0 206 232 336 642.2 0 336 233 331 642.2 0 331 234 180 642.2 0 180 235 149 642.2 0 149 236 161 642.2 0 161 237 103 642.2 0 103 238 67 642.2 0 67 239 75 642.2 0 75 240 78 642.2 0 78 241 86 642.2 0 86 242 91 642.2 0 91 243 97 642.2 0 97 244 126 642.2 0 126 245 136 642.2 0 136 246 97 642.2 0 97 247 73 642.2 0 73 248 73 642.2 0 73 249 69 642.2 0 69 250 48 642.2 0 48 251 83 642.2 0 83 252 77 642.2 0 77 253 126 642.2 0 126 254 131 642.2 0 131 255 131 642.2 0 131 256 99 642.2 0 99 257 105 642.2 0 105 258 119 642.2 0 119 259 104 642.2 0 104 260 125 642.2 0 125 261 93 642.2 0 93 262 78 642.2 0 78 263 169 642.2 0 169 264 418 642.2 0 418 265 181 642.2 0 181 266 222 642.2 0 222 267 95 642.2 0 95 268 67 642.2 0 67 269 108 642.2 0 108 270 120 642.2 0 120 271 112 642.2 0 112 272 99 642.2 0 99 273 110 642.2 0 110 274 91 642.2 0 91 275 97 642.2 0 97 276 94 642.2 0 94 277 113 642.2 0 113 278 103 642.2 0 103 279 122 642.2 0 122 280 109 642.2 0 109 281 122 642.2 0 122 282 127 642.2 0 127 283 156 642.2 0 156 284 151 642.2 0 151 285 170 642.2 0 170 286 141 642.2 0 141 287 104 642.2 0 104 288 95 642.2 0 95 289 95 642.2 0 95 290 113 642.2 0 113 291 98 642.2 0 98 292 113 642.2 0 113 293 135 642.2 0 135 294 115 642.2 0 115 295 166 642.2 0 166 296 187 642.2 0 187 297 233 642.2 0 233 298 123 642.2 0 123 299 117 642.2 0 117 300 99 642.2 0 99 301 79 642.2 0 79 Finished in 3.82 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 117,094 Reads with adapters: 1,361 (1.2%) Reads that were too short: 280 (0.2%) Reads written (passing filters): 1,081 (0.9%) Total basepairs processed: 20,004,331 bp Total written (filtered): 247,011 bp (1.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1361 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 1 2 16 10 0.0 3 0 8 2 17 736 0.0 3 678 43 14 1 18 3 0.0 3 0 0 0 3 19 3 0.0 3 0 0 2 1 20 3 0.0 3 3 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 29 0.0 3 1 0 0 28 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 33 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 36 5 0.0 3 0 0 0 5 43 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 1 48 167 0.0 3 0 0 1 166 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 1 1 51 28 0.0 3 0 0 0 28 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 6 0.0 3 0 0 0 6 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 22 0.0 3 0 0 0 22 81 5 0.0 3 0 0 0 5 82 5 0.0 3 0 0 0 5 83 1 0.0 3 0 0 1 85 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 3 0.0 3 0 0 0 3 96 4 0.0 3 0 0 0 4 98 1 0.0 3 0 0 0 1 99 36 0.0 3 0 0 0 36 100 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 135 41 0.0 3 0 0 0 41 138 1 0.0 3 0 0 0 1 139 4 0.0 3 0 0 0 4 140 5 0.0 3 0 0 0 5 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 21 0.0 3 0 0 1 20 150 118 0.0 3 0 0 8 110 151 5 0.0 3 0 0 0 5 157 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 164 12 0.0 3 0 0 0 12 165 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 214 3 0.0 3 0 0 0 3 215 3 0.0 3 0 0 0 3 234 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (28 us/read; 2.16 M reads/minute). === Summary === Total reads processed: 1,081 Reads with adapters: 9 (0.8%) Reads written (passing filters): 1,081 (100.0%) Total basepairs processed: 247,011 bp Total written (filtered): 246,984 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 9 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.1% C: 44.4% G: 44.4% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 16.9 0 9