This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.16 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 179,280 (7.3%) Reads that were too short: 41,936 (1.7%) Reads written (passing filters): 137,344 (5.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 26,401,802 bp (3.6%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 179280 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 286 596.3 0 286 7 9361 596.3 0 9361 8 8258 596.3 0 8258 9 23 596.3 0 23 10 24 596.3 0 24 11 59 596.3 0 59 12 29 596.3 0 29 13 30 596.3 0 30 14 17 596.3 0 17 15 23 596.3 0 23 16 29 596.3 0 29 17 33 596.3 0 33 18 22 596.3 0 22 19 26 596.3 0 26 20 36 596.3 0 36 21 25 596.3 0 25 22 21 596.3 0 21 23 33 596.3 0 33 24 30 596.3 0 30 25 29 596.3 0 29 26 27 596.3 0 27 27 38 596.3 0 38 28 48 596.3 0 48 29 40 596.3 0 40 30 213 596.3 0 213 31 111 596.3 0 111 32 23 596.3 0 23 33 27 596.3 0 27 34 31 596.3 0 31 35 27 596.3 0 27 36 54 596.3 0 54 37 50 596.3 0 50 38 45 596.3 0 45 39 43 596.3 0 43 40 39 596.3 0 39 41 30 596.3 0 30 42 45 596.3 0 45 43 50 596.3 0 50 44 42 596.3 0 42 45 35 596.3 0 35 46 30 596.3 0 30 47 44 596.3 0 44 48 34 596.3 0 34 49 36 596.3 0 36 50 33 596.3 0 33 51 30 596.3 0 30 52 32 596.3 0 32 53 41 596.3 0 41 54 42 596.3 0 42 55 45 596.3 0 45 56 44 596.3 0 44 57 42 596.3 0 42 58 58 596.3 0 58 59 59 596.3 0 59 60 51 596.3 0 51 61 69 596.3 0 69 62 46 596.3 0 46 63 61 596.3 0 61 64 43 596.3 0 43 65 56 596.3 0 56 66 90 596.3 0 90 67 55 596.3 0 55 68 69 596.3 0 69 69 91 596.3 0 91 70 850 596.3 0 850 71 1088 596.3 0 1088 72 856 596.3 0 856 73 65 596.3 0 65 74 47 596.3 0 47 75 42 596.3 0 42 76 56 596.3 0 56 77 51 596.3 0 51 78 78 596.3 0 78 79 81 596.3 0 81 80 85 596.3 0 85 81 128 596.3 0 128 82 272 596.3 0 272 83 170 596.3 0 170 84 61 596.3 0 61 85 54 596.3 0 54 86 58 596.3 0 58 87 54 596.3 0 54 88 41 596.3 0 41 89 50 596.3 0 50 90 35 596.3 0 35 91 37 596.3 0 37 92 44 596.3 0 44 93 36 596.3 0 36 94 52 596.3 0 52 95 30 596.3 0 30 96 30 596.3 0 30 97 67 596.3 0 67 98 96 596.3 0 96 99 112 596.3 0 112 100 152 596.3 0 152 101 95 596.3 0 95 102 72 596.3 0 72 103 64 596.3 0 64 104 71 596.3 0 71 105 62 596.3 0 62 106 72 596.3 0 72 107 124 596.3 0 124 108 98 596.3 0 98 109 219 596.3 0 219 110 347 596.3 0 347 111 716 596.3 0 716 112 208 596.3 0 208 113 256 596.3 0 256 114 532 596.3 0 532 115 252 596.3 0 252 116 244 596.3 0 244 117 408 596.3 0 408 118 393 596.3 0 393 119 602 596.3 0 602 120 501 596.3 0 501 121 655 596.3 0 655 122 854 596.3 0 854 123 1443 596.3 0 1443 124 21044 596.3 0 21044 125 39124 596.3 0 39124 126 31035 596.3 0 31035 127 285 596.3 0 285 128 104 596.3 0 104 129 252 596.3 0 252 130 119 596.3 0 119 131 98 596.3 0 98 132 48 596.3 0 48 133 57 596.3 0 57 134 65 596.3 0 65 135 73 596.3 0 73 136 111 596.3 0 111 137 96 596.3 0 96 138 259 596.3 0 259 139 314 596.3 0 314 140 94 596.3 0 94 141 101 596.3 0 101 142 137 596.3 0 137 143 87 596.3 0 87 144 95 596.3 0 95 145 82 596.3 0 82 146 108 596.3 0 108 147 105 596.3 0 105 148 167 596.3 0 167 149 1534 596.3 0 1534 150 1646 596.3 0 1646 151 2105 596.3 0 2105 152 85 596.3 0 85 153 39 596.3 0 39 154 52 596.3 0 52 155 57 596.3 0 57 156 68 596.3 0 68 157 32 596.3 0 32 158 79 596.3 0 79 159 78 596.3 0 78 160 49 596.3 0 49 161 60 596.3 0 60 162 82 596.3 0 82 163 129 596.3 0 129 164 280 596.3 0 280 165 72 596.3 0 72 166 83 596.3 0 83 167 38 596.3 0 38 168 50 596.3 0 50 169 56 596.3 0 56 170 42 596.3 0 42 171 58 596.3 0 58 172 33 596.3 0 33 173 39 596.3 0 39 174 32 596.3 0 32 175 45 596.3 0 45 176 48 596.3 0 48 177 59 596.3 0 59 178 46 596.3 0 46 179 44 596.3 0 44 180 63 596.3 0 63 181 143 596.3 0 143 182 214 596.3 0 214 183 416 596.3 0 416 184 83 596.3 0 83 185 86 596.3 0 86 186 84 596.3 0 84 187 47 596.3 0 47 188 113 596.3 0 113 189 261 596.3 0 261 190 258 596.3 0 258 191 46 596.3 0 46 192 41 596.3 0 41 193 37 596.3 0 37 194 55 596.3 0 55 195 56 596.3 0 56 196 69 596.3 0 69 197 56 596.3 0 56 198 104 596.3 0 104 199 62 596.3 0 62 200 47 596.3 0 47 201 44 596.3 0 44 202 44 596.3 0 44 203 70 596.3 0 70 204 92 596.3 0 92 205 116 596.3 0 116 206 76 596.3 0 76 207 132 596.3 0 132 208 183 596.3 0 183 209 92 596.3 0 92 210 106 596.3 0 106 211 91 596.3 0 91 212 141 596.3 0 141 213 241 596.3 0 241 214 188 596.3 0 188 215 91 596.3 0 91 216 85 596.3 0 85 217 62 596.3 0 62 218 123 596.3 0 123 219 137 596.3 0 137 220 218 596.3 0 218 221 249 596.3 0 249 222 310 596.3 0 310 223 860 596.3 0 860 224 1296 596.3 0 1296 225 11094 596.3 0 11094 226 7240 596.3 0 7240 227 13541 596.3 0 13541 228 96 596.3 0 96 229 45 596.3 0 45 230 52 596.3 0 52 231 43 596.3 0 43 232 72 596.3 0 72 233 62 596.3 0 62 234 71 596.3 0 71 235 93 596.3 0 93 236 101 596.3 0 101 237 65 596.3 0 65 238 69 596.3 0 69 239 66 596.3 0 66 240 59 596.3 0 59 241 45 596.3 0 45 242 63 596.3 0 63 243 114 596.3 0 114 244 142 596.3 0 142 245 147 596.3 0 147 246 77 596.3 0 77 247 45 596.3 0 45 248 39 596.3 0 39 249 31 596.3 0 31 250 36 596.3 0 36 251 50 596.3 0 50 252 46 596.3 0 46 253 66 596.3 0 66 254 77 596.3 0 77 255 89 596.3 0 89 256 105 596.3 0 105 257 73 596.3 0 73 258 95 596.3 0 95 259 69 596.3 0 69 260 87 596.3 0 87 261 59 596.3 0 59 262 80 596.3 0 80 263 121 596.3 0 121 264 111 596.3 0 111 265 100 596.3 0 100 266 100 596.3 0 100 267 113 596.3 0 113 268 185 596.3 0 185 269 98 596.3 0 98 270 51 596.3 0 51 271 40 596.3 0 40 272 49 596.3 0 49 273 66 596.3 0 66 274 77 596.3 0 77 275 66 596.3 0 66 276 50 596.3 0 50 277 58 596.3 0 58 278 60 596.3 0 60 279 62 596.3 0 62 280 74 596.3 0 74 281 57 596.3 0 57 282 49 596.3 0 49 283 43 596.3 0 43 284 64 596.3 0 64 285 49 596.3 0 49 286 40 596.3 0 40 287 38 596.3 0 38 288 37 596.3 0 37 289 64 596.3 0 64 290 46 596.3 0 46 291 34 596.3 0 34 292 35 596.3 0 35 293 44 596.3 0 44 294 51 596.3 0 51 295 45 596.3 0 45 296 54 596.3 0 54 297 49 596.3 0 49 298 75 596.3 0 75 299 58 596.3 0 58 300 41 596.3 0 41 301 75 596.3 0 75 Finished in 4.95 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 137,344 Reads with adapters: 10,870 (7.9%) Reads that were too short: 343 (0.2%) Reads written (passing filters): 10,527 (7.7%) Total basepairs processed: 26,401,802 bp Total written (filtered): 2,717,894 bp (10.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10870 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 4 2 16 114 0.0 3 0 92 13 9 17 5172 0.0 3 4831 291 42 8 18 3960 0.0 3 3674 234 46 6 19 24 0.0 3 0 14 5 5 20 23 0.0 3 0 0 11 12 21 15 0.0 3 0 0 0 15 22 1 0.0 3 0 0 0 1 23 4 0.0 3 0 0 0 4 26 13 0.0 3 0 0 0 13 28 1 0.0 3 0 0 0 1 29 5 0.0 3 0 0 0 5 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 384 0.0 3 0 0 1 383 43 1 0.0 3 0 0 0 1 45 70 0.0 3 1 0 3 66 47 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 1 52 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 5 0.0 3 0 0 1 4 66 51 0.0 3 0 0 2 49 68 630 0.0 3 0 0 1 629 69 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 1 0.0 3 1 73 1 0.0 3 1 75 2 0.0 3 1 0 0 1 76 2 0.0 3 0 1 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 93 54 0.0 3 0 0 1 53 94 4 0.0 3 0 0 0 4 95 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 102 59 0.0 3 0 0 0 59 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 4 0.0 3 0 0 0 4 108 2 0.0 3 0 0 0 2 110 6 0.0 3 0 0 0 6 111 13 0.0 3 0 0 0 13 113 5 0.0 3 0 0 0 5 114 1 0.0 3 0 0 0 1 115 6 0.0 3 0 0 0 6 117 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 140 4 0.0 3 0 0 0 4 142 20 0.0 3 0 0 0 20 143 25 0.0 3 0 0 1 24 144 34 0.0 3 0 1 0 33 145 3 0.0 3 0 0 0 3 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 10 0.0 3 0 0 0 10 155 10 0.0 3 0 0 0 10 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 167 7 0.0 3 0 0 0 7 168 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 1 1 173 2 0.0 3 0 0 0 2 174 4 0.0 3 0 0 0 4 175 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 3 0.0 3 0 0 0 3 217 1 0.0 3 0 0 0 1 218 12 0.0 3 0 0 0 12 221 4 0.0 3 0 0 0 4 225 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.37 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 10,527 Reads with adapters: 171 (1.6%) Reads written (passing filters): 10,527 (100.0%) Total basepairs processed: 2,717,894 bp Total written (filtered): 2,717,359 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 171 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 30.4% C: 33.3% G: 25.7% T: 10.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 156 164.5 0 156 4 11 41.1 0 11 5 3 10.3 0 3 8 1 0.2 0 1