This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 148.69 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 608,845 (19.2%) Reads that were too short: 7,262 (0.2%) Reads written (passing filters): 601,583 (19.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 112,668,347 bp (11.8%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 608845 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 76 773.1 0 76 7 89 773.1 0 89 8 653 773.1 0 653 9 887 773.1 0 887 10 61 773.1 0 61 11 73 773.1 0 73 12 67 773.1 0 67 13 68 773.1 0 68 14 82 773.1 0 82 15 85 773.1 0 85 16 70 773.1 0 70 17 106 773.1 0 106 18 96 773.1 0 96 19 71 773.1 0 71 20 58 773.1 0 58 21 70 773.1 0 70 22 71 773.1 0 71 23 73 773.1 0 73 24 83 773.1 0 83 25 180 773.1 0 180 26 139 773.1 0 139 27 128 773.1 0 128 28 64 773.1 0 64 29 69 773.1 0 69 30 79 773.1 0 79 31 90 773.1 0 90 32 94 773.1 0 94 33 52 773.1 0 52 34 68 773.1 0 68 35 331 773.1 0 331 36 78 773.1 0 78 37 68 773.1 0 68 38 69 773.1 0 69 39 74 773.1 0 74 40 68 773.1 0 68 41 50 773.1 0 50 42 71 773.1 0 71 43 60 773.1 0 60 44 84 773.1 0 84 45 65 773.1 0 65 46 59 773.1 0 59 47 62 773.1 0 62 48 49 773.1 0 49 49 49 773.1 0 49 50 46 773.1 0 46 51 45 773.1 0 45 52 70 773.1 0 70 53 65 773.1 0 65 54 53 773.1 0 53 55 115 773.1 0 115 56 88 773.1 0 88 57 79 773.1 0 79 58 80 773.1 0 80 59 61 773.1 0 61 60 120 773.1 0 120 61 365 773.1 0 365 62 65 773.1 0 65 63 61 773.1 0 61 64 77 773.1 0 77 65 44 773.1 0 44 66 71 773.1 0 71 67 85 773.1 0 85 68 92 773.1 0 92 69 154 773.1 0 154 70 1128 773.1 0 1128 71 1010 773.1 0 1010 72 1139 773.1 0 1139 73 77 773.1 0 77 74 81 773.1 0 81 75 74 773.1 0 74 76 155 773.1 0 155 77 134 773.1 0 134 78 94 773.1 0 94 79 151 773.1 0 151 80 112 773.1 0 112 81 110 773.1 0 110 82 256 773.1 0 256 83 201 773.1 0 201 84 234 773.1 0 234 85 154 773.1 0 154 86 759 773.1 0 759 87 99 773.1 0 99 88 68 773.1 0 68 89 92 773.1 0 92 90 78 773.1 0 78 91 62 773.1 0 62 92 85 773.1 0 85 93 143 773.1 0 143 94 112 773.1 0 112 95 352 773.1 0 352 96 827 773.1 0 827 97 171 773.1 0 171 98 351 773.1 0 351 99 226 773.1 0 226 100 505 773.1 0 505 101 1381 773.1 0 1381 102 356 773.1 0 356 103 281 773.1 0 281 104 148 773.1 0 148 105 278 773.1 0 278 106 485 773.1 0 485 107 555 773.1 0 555 108 1047 773.1 0 1047 109 1500 773.1 0 1500 110 2239 773.1 0 2239 111 4951 773.1 0 4951 112 10694 773.1 0 10694 113 218948 773.1 0 218948 114 138621 773.1 0 138621 115 172514 773.1 0 172514 116 787 773.1 0 787 117 98 773.1 0 98 118 98 773.1 0 98 119 69 773.1 0 69 120 72 773.1 0 72 121 125 773.1 0 125 122 267 773.1 0 267 123 93 773.1 0 93 124 97 773.1 0 97 125 140 773.1 0 140 126 123 773.1 0 123 127 622 773.1 0 622 128 185 773.1 0 185 129 137 773.1 0 137 130 113 773.1 0 113 131 138 773.1 0 138 132 178 773.1 0 178 133 184 773.1 0 184 134 262 773.1 0 262 135 4454 773.1 0 4454 136 8097 773.1 0 8097 137 9364 773.1 0 9364 138 237 773.1 0 237 139 256 773.1 0 256 140 313 773.1 0 313 141 384 773.1 0 384 142 55 773.1 0 55 143 78 773.1 0 78 144 88 773.1 0 88 145 581 773.1 0 581 146 182 773.1 0 182 147 78 773.1 0 78 148 57 773.1 0 57 149 75 773.1 0 75 150 52 773.1 0 52 151 48 773.1 0 48 152 48 773.1 0 48 153 63 773.1 0 63 154 141 773.1 0 141 155 62 773.1 0 62 156 78 773.1 0 78 157 48 773.1 0 48 158 132 773.1 0 132 159 131 773.1 0 131 160 156 773.1 0 156 161 73 773.1 0 73 162 55 773.1 0 55 163 49 773.1 0 49 164 64 773.1 0 64 165 38 773.1 0 38 166 42 773.1 0 42 167 42 773.1 0 42 168 47 773.1 0 47 169 60 773.1 0 60 170 68 773.1 0 68 171 45 773.1 0 45 172 44 773.1 0 44 173 49 773.1 0 49 174 36 773.1 0 36 175 55 773.1 0 55 176 43 773.1 0 43 177 74 773.1 0 74 178 62 773.1 0 62 179 67 773.1 0 67 180 160 773.1 0 160 181 114 773.1 0 114 182 84 773.1 0 84 183 56 773.1 0 56 184 46 773.1 0 46 185 48 773.1 0 48 186 52 773.1 0 52 187 37 773.1 0 37 188 67 773.1 0 67 189 59 773.1 0 59 190 82 773.1 0 82 191 69 773.1 0 69 192 212 773.1 0 212 193 48 773.1 0 48 194 48 773.1 0 48 195 49 773.1 0 49 196 67 773.1 0 67 197 50 773.1 0 50 198 52 773.1 0 52 199 72 773.1 0 72 200 51 773.1 0 51 201 88 773.1 0 88 202 54 773.1 0 54 203 98 773.1 0 98 204 127 773.1 0 127 205 88 773.1 0 88 206 174 773.1 0 174 207 50 773.1 0 50 208 70 773.1 0 70 209 55 773.1 0 55 210 37 773.1 0 37 211 38 773.1 0 38 212 46 773.1 0 46 213 50 773.1 0 50 214 60 773.1 0 60 215 64 773.1 0 64 216 86 773.1 0 86 217 34 773.1 0 34 218 37 773.1 0 37 219 128 773.1 0 128 220 81 773.1 0 81 221 120 773.1 0 120 222 58 773.1 0 58 223 71 773.1 0 71 224 528 773.1 0 528 225 610 773.1 0 610 226 341 773.1 0 341 227 53 773.1 0 53 228 37 773.1 0 37 229 52 773.1 0 52 230 42 773.1 0 42 231 37 773.1 0 37 232 29 773.1 0 29 233 52 773.1 0 52 234 61 773.1 0 61 235 73 773.1 0 73 236 56 773.1 0 56 237 44 773.1 0 44 238 37 773.1 0 37 239 32 773.1 0 32 240 37 773.1 0 37 241 49 773.1 0 49 242 27 773.1 0 27 243 30 773.1 0 30 244 45 773.1 0 45 245 53 773.1 0 53 246 46 773.1 0 46 247 36 773.1 0 36 248 39 773.1 0 39 249 36 773.1 0 36 250 53 773.1 0 53 251 42 773.1 0 42 252 51 773.1 0 51 253 39 773.1 0 39 254 48 773.1 0 48 255 61 773.1 0 61 256 51 773.1 0 51 257 65 773.1 0 65 258 60 773.1 0 60 259 60 773.1 0 60 260 51 773.1 0 51 261 58 773.1 0 58 262 30 773.1 0 30 263 41 773.1 0 41 264 33 773.1 0 33 265 47 773.1 0 47 266 35 773.1 0 35 267 40 773.1 0 40 268 46 773.1 0 46 269 52 773.1 0 52 270 54 773.1 0 54 271 92 773.1 0 92 272 72 773.1 0 72 273 158 773.1 0 158 274 85 773.1 0 85 275 53 773.1 0 53 276 43 773.1 0 43 277 51 773.1 0 51 278 49 773.1 0 49 279 56 773.1 0 56 280 112 773.1 0 112 281 122 773.1 0 122 282 190 773.1 0 190 283 41 773.1 0 41 284 68 773.1 0 68 285 80 773.1 0 80 286 52 773.1 0 52 287 41 773.1 0 41 288 51 773.1 0 51 289 48 773.1 0 48 290 39 773.1 0 39 291 39 773.1 0 39 292 31 773.1 0 31 293 68 773.1 0 68 294 107 773.1 0 107 295 77 773.1 0 77 296 111 773.1 0 111 297 63 773.1 0 63 298 35 773.1 0 35 299 38 773.1 0 38 300 36 773.1 0 36 301 29 773.1 0 29 Finished in 33.83 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 601,583 Reads with adapters: 877 (0.1%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 860 (0.1%) Total basepairs processed: 112,668,347 bp Total written (filtered): 233,485 bp (0.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 877 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 19 1 0.0 3 0 1 20 848 0.0 4 838 8 2 21 2 0.0 4 2 22 1 0.0 4 1 23 2 0.0 4 1 0 0 0 1 27 1 0.0 4 1 49 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 1 122 3 0.0 4 0 0 0 0 3 123 3 0.0 4 1 0 0 1 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 136 1 0.0 4 1 158 1 0.0 4 1 177 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 1 187 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 860 Reads with adapters: 410 (47.7%) Reads written (passing filters): 860 (100.0%) Total basepairs processed: 233,485 bp Total written (filtered): 229,252 bp (98.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 410 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.2% C: 1.0% G: 96.8% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 387 0.0 0 9 378 10 21 0.0 1 0 21 270 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.