This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 150.15 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 194,905 (6.2%) Reads that were too short: 12,969 (0.4%) Reads written (passing filters): 181,936 (5.7%) Total basepairs processed: 953,096,634 bp Total written (filtered): 30,154,625 bp (3.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 194905 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 156 773.1 0 156 7 1919 773.1 0 1919 8 1837 773.1 0 1837 9 114 773.1 0 114 10 55 773.1 0 55 11 66 773.1 0 66 12 68 773.1 0 68 13 58 773.1 0 58 14 78 773.1 0 78 15 85 773.1 0 85 16 53 773.1 0 53 17 74 773.1 0 74 18 65 773.1 0 65 19 45 773.1 0 45 20 68 773.1 0 68 21 76 773.1 0 76 22 66 773.1 0 66 23 71 773.1 0 71 24 58 773.1 0 58 25 81 773.1 0 81 26 92 773.1 0 92 27 189 773.1 0 189 28 260 773.1 0 260 29 276 773.1 0 276 30 57 773.1 0 57 31 64 773.1 0 64 32 199 773.1 0 199 33 561 773.1 0 561 34 72 773.1 0 72 35 95 773.1 0 95 36 76 773.1 0 76 37 73 773.1 0 73 38 58 773.1 0 58 39 85 773.1 0 85 40 51 773.1 0 51 41 63 773.1 0 63 42 74 773.1 0 74 43 308 773.1 0 308 44 60 773.1 0 60 45 67 773.1 0 67 46 79 773.1 0 79 47 82 773.1 0 82 48 68 773.1 0 68 49 102 773.1 0 102 50 69 773.1 0 69 51 74 773.1 0 74 52 89 773.1 0 89 53 81 773.1 0 81 54 64 773.1 0 64 55 69 773.1 0 69 56 59 773.1 0 59 57 86 773.1 0 86 58 360 773.1 0 360 59 308 773.1 0 308 60 787 773.1 0 787 61 64 773.1 0 64 62 60 773.1 0 60 63 51 773.1 0 51 64 49 773.1 0 49 65 56 773.1 0 56 66 65 773.1 0 65 67 57 773.1 0 57 68 76 773.1 0 76 69 91 773.1 0 91 70 197 773.1 0 197 71 169 773.1 0 169 72 1127 773.1 0 1127 73 693 773.1 0 693 74 705 773.1 0 705 75 87 773.1 0 87 76 88 773.1 0 88 77 91 773.1 0 91 78 69 773.1 0 69 79 63 773.1 0 63 80 75 773.1 0 75 81 64 773.1 0 64 82 70 773.1 0 70 83 88 773.1 0 88 84 60 773.1 0 60 85 50 773.1 0 50 86 58 773.1 0 58 87 74 773.1 0 74 88 88 773.1 0 88 89 57 773.1 0 57 90 136 773.1 0 136 91 127 773.1 0 127 92 152 773.1 0 152 93 101 773.1 0 101 94 62 773.1 0 62 95 76 773.1 0 76 96 70 773.1 0 70 97 49 773.1 0 49 98 73 773.1 0 73 99 65 773.1 0 65 100 84 773.1 0 84 101 92 773.1 0 92 102 52 773.1 0 52 103 57 773.1 0 57 104 75 773.1 0 75 105 63 773.1 0 63 106 56 773.1 0 56 107 77 773.1 0 77 108 84 773.1 0 84 109 109 773.1 0 109 110 107 773.1 0 107 111 97 773.1 0 97 112 262 773.1 0 262 113 333 773.1 0 333 114 415 773.1 0 415 115 66 773.1 0 66 116 88 773.1 0 88 117 160 773.1 0 160 118 82 773.1 0 82 119 196 773.1 0 196 120 90 773.1 0 90 121 98 773.1 0 98 122 74 773.1 0 74 123 98 773.1 0 98 124 76 773.1 0 76 125 109 773.1 0 109 126 140 773.1 0 140 127 184 773.1 0 184 128 274 773.1 0 274 129 573 773.1 0 573 130 952 773.1 0 952 131 226 773.1 0 226 132 204 773.1 0 204 133 348 773.1 0 348 134 278 773.1 0 278 135 253 773.1 0 253 136 441 773.1 0 441 137 355 773.1 0 355 138 494 773.1 0 494 139 438 773.1 0 438 140 872 773.1 0 872 141 1353 773.1 0 1353 142 2043 773.1 0 2043 143 51590 773.1 0 51590 144 36314 773.1 0 36314 145 60471 773.1 0 60471 146 336 773.1 0 336 147 83 773.1 0 83 148 227 773.1 0 227 149 183 773.1 0 183 150 182 773.1 0 182 151 46 773.1 0 46 152 68 773.1 0 68 153 60 773.1 0 60 154 43 773.1 0 43 155 104 773.1 0 104 156 195 773.1 0 195 157 156 773.1 0 156 158 55 773.1 0 55 159 55 773.1 0 55 160 59 773.1 0 59 161 68 773.1 0 68 162 102 773.1 0 102 163 377 773.1 0 377 164 161 773.1 0 161 165 499 773.1 0 499 166 66 773.1 0 66 167 58 773.1 0 58 168 67 773.1 0 67 169 48 773.1 0 48 170 64 773.1 0 64 171 57 773.1 0 57 172 50 773.1 0 50 173 59 773.1 0 59 174 76 773.1 0 76 175 43 773.1 0 43 176 89 773.1 0 89 177 58 773.1 0 58 178 61 773.1 0 61 179 55 773.1 0 55 180 65 773.1 0 65 181 64 773.1 0 64 182 49 773.1 0 49 183 76 773.1 0 76 184 70 773.1 0 70 185 60 773.1 0 60 186 61 773.1 0 61 187 57 773.1 0 57 188 62 773.1 0 62 189 65 773.1 0 65 190 82 773.1 0 82 191 90 773.1 0 90 192 54 773.1 0 54 193 58 773.1 0 58 194 41 773.1 0 41 195 77 773.1 0 77 196 87 773.1 0 87 197 67 773.1 0 67 198 61 773.1 0 61 199 54 773.1 0 54 200 59 773.1 0 59 201 61 773.1 0 61 202 65 773.1 0 65 203 101 773.1 0 101 204 98 773.1 0 98 205 78 773.1 0 78 206 77 773.1 0 77 207 74 773.1 0 74 208 139 773.1 0 139 209 141 773.1 0 141 210 130 773.1 0 130 211 171 773.1 0 171 212 119 773.1 0 119 213 245 773.1 0 245 214 312 773.1 0 312 215 881 773.1 0 881 216 499 773.1 0 499 217 571 773.1 0 571 218 64 773.1 0 64 219 49 773.1 0 49 220 63 773.1 0 63 221 88 773.1 0 88 222 92 773.1 0 92 223 83 773.1 0 83 224 80 773.1 0 80 225 86 773.1 0 86 226 92 773.1 0 92 227 113 773.1 0 113 228 84 773.1 0 84 229 74 773.1 0 74 230 62 773.1 0 62 231 139 773.1 0 139 232 211 773.1 0 211 233 235 773.1 0 235 234 120 773.1 0 120 235 91 773.1 0 91 236 123 773.1 0 123 237 93 773.1 0 93 238 53 773.1 0 53 239 74 773.1 0 74 240 56 773.1 0 56 241 86 773.1 0 86 242 73 773.1 0 73 243 80 773.1 0 80 244 105 773.1 0 105 245 122 773.1 0 122 246 95 773.1 0 95 247 61 773.1 0 61 248 59 773.1 0 59 249 62 773.1 0 62 250 53 773.1 0 53 251 86 773.1 0 86 252 67 773.1 0 67 253 88 773.1 0 88 254 87 773.1 0 87 255 86 773.1 0 86 256 56 773.1 0 56 257 69 773.1 0 69 258 92 773.1 0 92 259 671 773.1 0 671 260 251 773.1 0 251 261 120 773.1 0 120 262 67 773.1 0 67 263 181 773.1 0 181 264 189 773.1 0 189 265 212 773.1 0 212 266 89 773.1 0 89 267 53 773.1 0 53 268 56 773.1 0 56 269 77 773.1 0 77 270 89 773.1 0 89 271 103 773.1 0 103 272 83 773.1 0 83 273 84 773.1 0 84 274 65 773.1 0 65 275 88 773.1 0 88 276 79 773.1 0 79 277 76 773.1 0 76 278 97 773.1 0 97 279 74 773.1 0 74 280 174 773.1 0 174 281 141 773.1 0 141 282 209 773.1 0 209 283 109 773.1 0 109 284 121 773.1 0 121 285 120 773.1 0 120 286 128 773.1 0 128 287 80 773.1 0 80 288 72 773.1 0 72 289 81 773.1 0 81 290 91 773.1 0 91 291 105 773.1 0 105 292 100 773.1 0 100 293 99 773.1 0 99 294 112 773.1 0 112 295 175 773.1 0 175 296 227 773.1 0 227 297 304 773.1 0 304 298 148 773.1 0 148 299 84 773.1 0 84 300 64 773.1 0 64 301 68 773.1 0 68 Finished in 9.55 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 181,936 Reads with adapters: 2,393 (1.3%) Reads that were too short: 281 (0.2%) Reads written (passing filters): 2,112 (1.2%) Total basepairs processed: 30,154,625 bp Total written (filtered): 536,664 bp (1.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2393 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 2 1 16 23 0.0 3 0 20 2 1 17 1774 0.0 3 1657 98 17 2 18 8 0.0 3 1 2 1 4 20 4 0.0 3 3 0 0 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 2 0.0 3 0 1 0 1 28 16 0.0 3 0 0 0 16 31 2 0.0 3 1 0 0 1 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 1 38 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 146 0.0 3 0 0 0 146 49 2 0.0 3 2 50 3 0.0 3 3 51 48 0.0 3 0 0 1 47 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 13 0.0 3 0 0 0 13 62 2 0.0 3 0 0 1 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 1 67 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 1 72 4 0.0 3 0 0 0 4 74 6 0.0 3 0 0 0 6 76 2 0.0 3 0 0 0 2 79 10 0.0 3 0 0 0 10 82 3 0.0 3 0 0 1 2 85 2 0.0 3 0 0 1 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 1 2 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 53 0.0 3 0 0 0 53 100 1 0.0 3 0 0 0 1 101 6 0.0 3 0 0 0 6 104 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 124 11 0.0 3 0 0 3 8 129 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 1 1 135 33 0.0 3 0 0 0 33 137 1 0.0 3 0 0 0 1 139 4 0.0 3 0 0 0 4 140 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 149 25 0.0 3 0 0 2 23 150 103 0.0 3 0 0 5 98 151 4 0.0 3 0 0 0 4 155 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 164 14 0.0 3 0 0 0 14 165 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 2,112 Reads with adapters: 5 (0.2%) Reads written (passing filters): 2,112 (100.0%) Total basepairs processed: 536,664 bp Total written (filtered): 536,647 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.0% C: 0.0% G: 60.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 33.0 0 3 4 2 8.2 0 2