This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 149.81 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 194,216 (6.1%) Reads that were too short: 12,078 (0.4%) Reads written (passing filters): 182,138 (5.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 30,210,476 bp (3.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 194216 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 122 773.1 0 122 7 2010 773.1 0 2010 8 1873 773.1 0 1873 9 98 773.1 0 98 10 72 773.1 0 72 11 64 773.1 0 64 12 53 773.1 0 53 13 48 773.1 0 48 14 68 773.1 0 68 15 55 773.1 0 55 16 57 773.1 0 57 17 75 773.1 0 75 18 60 773.1 0 60 19 68 773.1 0 68 20 91 773.1 0 91 21 86 773.1 0 86 22 71 773.1 0 71 23 85 773.1 0 85 24 74 773.1 0 74 25 92 773.1 0 92 26 98 773.1 0 98 27 105 773.1 0 105 28 92 773.1 0 92 29 127 773.1 0 127 30 66 773.1 0 66 31 77 773.1 0 77 32 209 773.1 0 209 33 640 773.1 0 640 34 94 773.1 0 94 35 89 773.1 0 89 36 70 773.1 0 70 37 75 773.1 0 75 38 78 773.1 0 78 39 90 773.1 0 90 40 76 773.1 0 76 41 78 773.1 0 78 42 94 773.1 0 94 43 324 773.1 0 324 44 72 773.1 0 72 45 98 773.1 0 98 46 77 773.1 0 77 47 84 773.1 0 84 48 72 773.1 0 72 49 104 773.1 0 104 50 92 773.1 0 92 51 78 773.1 0 78 52 96 773.1 0 96 53 96 773.1 0 96 54 98 773.1 0 98 55 77 773.1 0 77 56 87 773.1 0 87 57 116 773.1 0 116 58 371 773.1 0 371 59 338 773.1 0 338 60 881 773.1 0 881 61 60 773.1 0 60 62 102 773.1 0 102 63 54 773.1 0 54 64 76 773.1 0 76 65 59 773.1 0 59 66 70 773.1 0 70 67 67 773.1 0 67 68 85 773.1 0 85 69 101 773.1 0 101 70 136 773.1 0 136 71 142 773.1 0 142 72 1053 773.1 0 1053 73 709 773.1 0 709 74 746 773.1 0 746 75 75 773.1 0 75 76 79 773.1 0 79 77 97 773.1 0 97 78 84 773.1 0 84 79 71 773.1 0 71 80 79 773.1 0 79 81 94 773.1 0 94 82 85 773.1 0 85 83 83 773.1 0 83 84 62 773.1 0 62 85 72 773.1 0 72 86 81 773.1 0 81 87 84 773.1 0 84 88 97 773.1 0 97 89 76 773.1 0 76 90 142 773.1 0 142 91 112 773.1 0 112 92 166 773.1 0 166 93 141 773.1 0 141 94 88 773.1 0 88 95 86 773.1 0 86 96 113 773.1 0 113 97 64 773.1 0 64 98 67 773.1 0 67 99 102 773.1 0 102 100 98 773.1 0 98 101 105 773.1 0 105 102 81 773.1 0 81 103 74 773.1 0 74 104 96 773.1 0 96 105 90 773.1 0 90 106 83 773.1 0 83 107 99 773.1 0 99 108 101 773.1 0 101 109 123 773.1 0 123 110 92 773.1 0 92 111 101 773.1 0 101 112 175 773.1 0 175 113 198 773.1 0 198 114 188 773.1 0 188 115 76 773.1 0 76 116 109 773.1 0 109 117 160 773.1 0 160 118 107 773.1 0 107 119 233 773.1 0 233 120 105 773.1 0 105 121 110 773.1 0 110 122 87 773.1 0 87 123 120 773.1 0 120 124 98 773.1 0 98 125 131 773.1 0 131 126 151 773.1 0 151 127 194 773.1 0 194 128 308 773.1 0 308 129 577 773.1 0 577 130 989 773.1 0 989 131 209 773.1 0 209 132 195 773.1 0 195 133 371 773.1 0 371 134 344 773.1 0 344 135 229 773.1 0 229 136 435 773.1 0 435 137 364 773.1 0 364 138 439 773.1 0 439 139 424 773.1 0 424 140 767 773.1 0 767 141 1220 773.1 0 1220 142 1628 773.1 0 1628 143 51463 773.1 0 51463 144 36471 773.1 0 36471 145 60469 773.1 0 60469 146 323 773.1 0 323 147 84 773.1 0 84 148 97 773.1 0 97 149 125 773.1 0 125 150 111 773.1 0 111 151 63 773.1 0 63 152 88 773.1 0 88 153 77 773.1 0 77 154 52 773.1 0 52 155 103 773.1 0 103 156 195 773.1 0 195 157 168 773.1 0 168 158 87 773.1 0 87 159 76 773.1 0 76 160 78 773.1 0 78 161 97 773.1 0 97 162 102 773.1 0 102 163 380 773.1 0 380 164 134 773.1 0 134 165 445 773.1 0 445 166 67 773.1 0 67 167 85 773.1 0 85 168 65 773.1 0 65 169 76 773.1 0 76 170 68 773.1 0 68 171 81 773.1 0 81 172 62 773.1 0 62 173 70 773.1 0 70 174 75 773.1 0 75 175 70 773.1 0 70 176 84 773.1 0 84 177 66 773.1 0 66 178 71 773.1 0 71 179 65 773.1 0 65 180 85 773.1 0 85 181 58 773.1 0 58 182 62 773.1 0 62 183 70 773.1 0 70 184 73 773.1 0 73 185 74 773.1 0 74 186 52 773.1 0 52 187 70 773.1 0 70 188 93 773.1 0 93 189 71 773.1 0 71 190 82 773.1 0 82 191 82 773.1 0 82 192 81 773.1 0 81 193 69 773.1 0 69 194 67 773.1 0 67 195 81 773.1 0 81 196 78 773.1 0 78 197 78 773.1 0 78 198 79 773.1 0 79 199 73 773.1 0 73 200 83 773.1 0 83 201 99 773.1 0 99 202 82 773.1 0 82 203 104 773.1 0 104 204 108 773.1 0 108 205 91 773.1 0 91 206 90 773.1 0 90 207 78 773.1 0 78 208 74 773.1 0 74 209 91 773.1 0 91 210 119 773.1 0 119 211 89 773.1 0 89 212 102 773.1 0 102 213 139 773.1 0 139 214 296 773.1 0 296 215 1067 773.1 0 1067 216 537 773.1 0 537 217 774 773.1 0 774 218 65 773.1 0 65 219 63 773.1 0 63 220 68 773.1 0 68 221 86 773.1 0 86 222 60 773.1 0 60 223 79 773.1 0 79 224 99 773.1 0 99 225 92 773.1 0 92 226 71 773.1 0 71 227 91 773.1 0 91 228 76 773.1 0 76 229 64 773.1 0 64 230 69 773.1 0 69 231 95 773.1 0 95 232 88 773.1 0 88 233 92 773.1 0 92 234 104 773.1 0 104 235 84 773.1 0 84 236 99 773.1 0 99 237 78 773.1 0 78 238 62 773.1 0 62 239 73 773.1 0 73 240 67 773.1 0 67 241 89 773.1 0 89 242 66 773.1 0 66 243 74 773.1 0 74 244 92 773.1 0 92 245 90 773.1 0 90 246 96 773.1 0 96 247 58 773.1 0 58 248 70 773.1 0 70 249 77 773.1 0 77 250 71 773.1 0 71 251 88 773.1 0 88 252 71 773.1 0 71 253 97 773.1 0 97 254 100 773.1 0 100 255 98 773.1 0 98 256 69 773.1 0 69 257 104 773.1 0 104 258 86 773.1 0 86 259 610 773.1 0 610 260 257 773.1 0 257 261 137 773.1 0 137 262 63 773.1 0 63 263 113 773.1 0 113 264 114 773.1 0 114 265 108 773.1 0 108 266 79 773.1 0 79 267 62 773.1 0 62 268 63 773.1 0 63 269 98 773.1 0 98 270 87 773.1 0 87 271 108 773.1 0 108 272 85 773.1 0 85 273 87 773.1 0 87 274 86 773.1 0 86 275 82 773.1 0 82 276 91 773.1 0 91 277 85 773.1 0 85 278 88 773.1 0 88 279 98 773.1 0 98 280 119 773.1 0 119 281 109 773.1 0 109 282 151 773.1 0 151 283 113 773.1 0 113 284 92 773.1 0 92 285 111 773.1 0 111 286 109 773.1 0 109 287 91 773.1 0 91 288 79 773.1 0 79 289 88 773.1 0 88 290 90 773.1 0 90 291 93 773.1 0 93 292 81 773.1 0 81 293 93 773.1 0 93 294 99 773.1 0 99 295 124 773.1 0 124 296 145 773.1 0 145 297 172 773.1 0 172 298 109 773.1 0 109 299 90 773.1 0 90 300 83 773.1 0 83 301 74 773.1 0 74 Finished in 10.56 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 182,138 Reads with adapters: 1,941 (1.1%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 1,910 (1.0%) Total basepairs processed: 30,210,476 bp Total written (filtered): 520,621 bp (1.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1941 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 17 0.0 3 0 17 20 1875 0.0 4 1844 31 21 5 0.0 4 0 4 0 0 1 23 1 0.0 4 0 0 0 1 31 1 0.0 4 1 33 2 0.0 4 2 40 1 0.0 4 0 0 0 0 1 50 1 0.0 4 1 51 2 0.0 4 2 52 2 0.0 4 1 1 53 1 0.0 4 1 86 1 0.0 4 0 0 0 0 1 99 3 0.0 4 0 0 0 0 3 102 1 0.0 4 0 0 0 0 1 146 16 0.0 4 0 0 0 0 16 147 6 0.0 4 0 0 0 0 6 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 0 0 2 159 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 1,910 Reads with adapters: 155 (8.1%) Reads written (passing filters): 1,910 (100.0%) Total basepairs processed: 520,621 bp Total written (filtered): 517,402 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 155 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 3.9% G: 91.0% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 29.8 0 4 4 1 7.5 0 1 5 1 1.9 0 1 10 3 0.0 1 0 3 11 140 0.0 1 0 140 270 2 0.0 1 1 1 272 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.