This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.20 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 327,227 (12.7%) Reads that were too short: 22,445 (0.9%) Reads written (passing filters): 304,782 (11.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 58,578,196 bp (7.6%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 327227 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 263 628.5 0 263 7 6825 628.5 0 6825 8 6540 628.5 0 6540 9 59 628.5 0 59 10 70 628.5 0 70 11 49 628.5 0 49 12 383 628.5 0 383 13 266 628.5 0 266 14 885 628.5 0 885 15 39 628.5 0 39 16 42 628.5 0 42 17 43 628.5 0 43 18 50 628.5 0 50 19 44 628.5 0 44 20 59 628.5 0 59 21 63 628.5 0 63 22 71 628.5 0 71 23 52 628.5 0 52 24 98 628.5 0 98 25 308 628.5 0 308 26 308 628.5 0 308 27 150 628.5 0 150 28 58 628.5 0 58 29 70 628.5 0 70 30 69 628.5 0 69 31 83 628.5 0 83 32 86 628.5 0 86 33 65 628.5 0 65 34 54 628.5 0 54 35 91 628.5 0 91 36 81 628.5 0 81 37 79 628.5 0 79 38 62 628.5 0 62 39 73 628.5 0 73 40 74 628.5 0 74 41 82 628.5 0 82 42 102 628.5 0 102 43 159 628.5 0 159 44 72 628.5 0 72 45 38 628.5 0 38 46 67 628.5 0 67 47 84 628.5 0 84 48 64 628.5 0 64 49 113 628.5 0 113 50 50 628.5 0 50 51 55 628.5 0 55 52 56 628.5 0 56 53 83 628.5 0 83 54 108 628.5 0 108 55 131 628.5 0 131 56 148 628.5 0 148 57 76 628.5 0 76 58 96 628.5 0 96 59 70 628.5 0 70 60 95 628.5 0 95 61 86 628.5 0 86 62 124 628.5 0 124 63 106 628.5 0 106 64 105 628.5 0 105 65 593 628.5 0 593 66 633 628.5 0 633 67 689 628.5 0 689 68 129 628.5 0 129 69 81 628.5 0 81 70 95 628.5 0 95 71 71 628.5 0 71 72 104 628.5 0 104 73 102 628.5 0 102 74 83 628.5 0 83 75 78 628.5 0 78 76 65 628.5 0 65 77 102 628.5 0 102 78 172 628.5 0 172 79 137 628.5 0 137 80 241 628.5 0 241 81 206 628.5 0 206 82 130 628.5 0 130 83 115 628.5 0 115 84 138 628.5 0 138 85 124 628.5 0 124 86 157 628.5 0 157 87 227 628.5 0 227 88 352 628.5 0 352 89 540 628.5 0 540 90 906 628.5 0 906 91 1723 628.5 0 1723 92 473 628.5 0 473 93 527 628.5 0 527 94 1001 628.5 0 1001 95 406 628.5 0 406 96 359 628.5 0 359 97 853 628.5 0 853 98 865 628.5 0 865 99 1236 628.5 0 1236 100 888 628.5 0 888 101 1380 628.5 0 1380 102 2020 628.5 0 2020 103 2843 628.5 0 2843 104 36627 628.5 0 36627 105 55970 628.5 0 55970 106 113895 628.5 0 113895 107 494 628.5 0 494 108 61 628.5 0 61 109 70 628.5 0 70 110 134 628.5 0 134 111 97 628.5 0 97 112 138 628.5 0 138 113 100 628.5 0 100 114 168 628.5 0 168 115 140 628.5 0 140 116 102 628.5 0 102 117 76 628.5 0 76 118 82 628.5 0 82 119 186 628.5 0 186 120 115 628.5 0 115 121 200 628.5 0 200 122 80 628.5 0 80 123 79 628.5 0 79 124 200 628.5 0 200 125 259 628.5 0 259 126 340 628.5 0 340 127 105 628.5 0 105 128 80 628.5 0 80 129 73 628.5 0 73 130 87 628.5 0 87 131 47 628.5 0 47 132 132 628.5 0 132 133 55 628.5 0 55 134 92 628.5 0 92 135 67 628.5 0 67 136 80 628.5 0 80 137 81 628.5 0 81 138 177 628.5 0 177 139 162 628.5 0 162 140 288 628.5 0 288 141 108 628.5 0 108 142 166 628.5 0 166 143 58 628.5 0 58 144 128 628.5 0 128 145 91 628.5 0 91 146 125 628.5 0 125 147 195 628.5 0 195 148 160 628.5 0 160 149 125 628.5 0 125 150 108 628.5 0 108 151 223 628.5 0 223 152 380 628.5 0 380 153 508 628.5 0 508 154 320 628.5 0 320 155 326 628.5 0 326 156 341 628.5 0 341 157 793 628.5 0 793 158 1134 628.5 0 1134 159 15373 628.5 0 15373 160 8998 628.5 0 8998 161 16286 628.5 0 16286 162 153 628.5 0 153 163 77 628.5 0 77 164 70 628.5 0 70 165 79 628.5 0 79 166 79 628.5 0 79 167 265 628.5 0 265 168 233 628.5 0 233 169 359 628.5 0 359 170 82 628.5 0 82 171 116 628.5 0 116 172 274 628.5 0 274 173 352 628.5 0 352 174 347 628.5 0 347 175 77 628.5 0 77 176 86 628.5 0 86 177 74 628.5 0 74 178 71 628.5 0 71 179 77 628.5 0 77 180 119 628.5 0 119 181 111 628.5 0 111 182 121 628.5 0 121 183 142 628.5 0 142 184 462 628.5 0 462 185 196 628.5 0 196 186 316 628.5 0 316 187 311 628.5 0 311 188 127 628.5 0 127 189 320 628.5 0 320 190 97 628.5 0 97 191 62 628.5 0 62 192 78 628.5 0 78 193 106 628.5 0 106 194 104 628.5 0 104 195 116 628.5 0 116 196 123 628.5 0 123 197 73 628.5 0 73 198 115 628.5 0 115 199 186 628.5 0 186 200 492 628.5 0 492 201 423 628.5 0 423 202 594 628.5 0 594 203 96 628.5 0 96 204 84 628.5 0 84 205 160 628.5 0 160 206 195 628.5 0 195 207 218 628.5 0 218 208 196 628.5 0 196 209 163 628.5 0 163 210 87 628.5 0 87 211 70 628.5 0 70 212 90 628.5 0 90 213 110 628.5 0 110 214 114 628.5 0 114 215 112 628.5 0 112 216 121 628.5 0 121 217 107 628.5 0 107 218 188 628.5 0 188 219 327 628.5 0 327 220 364 628.5 0 364 221 274 628.5 0 274 222 172 628.5 0 172 223 78 628.5 0 78 224 83 628.5 0 83 225 93 628.5 0 93 226 147 628.5 0 147 227 196 628.5 0 196 228 305 628.5 0 305 229 217 628.5 0 217 230 224 628.5 0 224 231 113 628.5 0 113 232 131 628.5 0 131 233 115 628.5 0 115 234 111 628.5 0 111 235 89 628.5 0 89 236 86 628.5 0 86 237 103 628.5 0 103 238 201 628.5 0 201 239 262 628.5 0 262 240 1722 628.5 0 1722 241 1028 628.5 0 1028 242 1770 628.5 0 1770 243 523 628.5 0 523 244 97 628.5 0 97 245 76 628.5 0 76 246 92 628.5 0 92 247 125 628.5 0 125 248 142 628.5 0 142 249 116 628.5 0 116 250 98 628.5 0 98 251 122 628.5 0 122 252 108 628.5 0 108 253 135 628.5 0 135 254 390 628.5 0 390 255 370 628.5 0 370 256 473 628.5 0 473 257 313 628.5 0 313 258 83 628.5 0 83 259 104 628.5 0 104 260 93 628.5 0 93 261 120 628.5 0 120 262 111 628.5 0 111 263 113 628.5 0 113 264 84 628.5 0 84 265 88 628.5 0 88 266 128 628.5 0 128 267 185 628.5 0 185 268 182 628.5 0 182 269 200 628.5 0 200 270 165 628.5 0 165 271 109 628.5 0 109 272 117 628.5 0 117 273 177 628.5 0 177 274 196 628.5 0 196 275 224 628.5 0 224 276 244 628.5 0 244 277 155 628.5 0 155 278 357 628.5 0 357 279 496 628.5 0 496 280 234 628.5 0 234 281 380 628.5 0 380 282 584 628.5 0 584 283 473 628.5 0 473 284 192 628.5 0 192 285 85 628.5 0 85 286 78 628.5 0 78 287 90 628.5 0 90 288 182 628.5 0 182 289 122 628.5 0 122 290 133 628.5 0 133 291 109 628.5 0 109 292 129 628.5 0 129 293 158 628.5 0 158 294 219 628.5 0 219 295 179 628.5 0 179 296 153 628.5 0 153 297 87 628.5 0 87 298 114 628.5 0 114 299 199 628.5 0 199 300 370 628.5 0 370 301 348 628.5 0 348 Finished in 10.57 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 304,782 Reads with adapters: 8,332 (2.7%) Reads that were too short: 595 (0.2%) Reads written (passing filters): 7,737 (2.5%) Total basepairs processed: 58,578,196 bp Total written (filtered): 1,972,706 bp (3.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8332 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 3 16 77 0.0 3 0 68 4 5 17 6541 0.0 3 5783 606 125 27 18 21 0.0 3 1 16 1 3 19 31 0.0 3 0 1 19 11 20 63 0.0 3 16 8 5 34 22 3 0.0 3 0 0 0 3 23 16 0.0 3 0 0 0 16 24 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 5 0.0 3 0 0 0 5 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 0 2 42 3 0.0 3 0 0 0 3 44 11 0.0 3 0 0 0 11 45 3 0.0 3 0 0 0 3 46 4 0.0 3 0 0 0 4 47 3 0.0 3 0 0 0 3 48 6 0.0 3 0 0 0 6 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 1 2 53 5 0.0 3 0 0 0 5 54 5 0.0 3 0 0 1 4 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 6 0.0 3 0 0 0 6 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 3 0.0 3 0 0 0 3 66 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 6 0.0 3 0 0 0 6 71 5 0.0 3 0 0 0 5 72 2 0.0 3 0 0 0 2 73 4 0.0 3 1 0 0 3 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 82 3 0.0 3 0 0 0 3 84 2 0.0 3 0 0 0 2 85 3 0.0 3 0 0 0 3 86 6 0.0 3 0 0 0 6 87 203 0.0 3 0 0 2 201 88 471 0.0 3 0 0 9 462 90 35 0.0 3 0 0 0 35 91 127 0.0 3 0 0 10 117 92 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 99 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 5 0.0 3 0 0 0 5 104 7 0.0 3 0 0 0 7 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 4 0.0 3 0 0 0 4 111 15 0.0 3 0 0 0 15 112 12 0.0 3 0 0 0 12 113 13 0.0 3 0 0 0 13 114 44 0.0 3 0 0 1 43 115 6 0.0 3 0 0 0 6 116 1 0.0 3 0 0 0 1 117 3 0.0 3 0 0 0 3 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 8 0.0 3 0 0 0 8 124 2 0.0 3 0 0 0 2 125 3 0.0 3 0 0 0 3 126 16 0.0 3 0 0 1 15 127 5 0.0 3 0 0 0 5 128 11 0.0 3 0 0 0 11 129 3 0.0 3 0 0 0 3 130 19 0.0 3 0 0 1 18 131 8 0.0 3 0 0 1 7 132 1 0.0 3 0 0 0 1 133 4 0.0 3 0 0 0 4 135 4 0.0 3 0 0 0 4 137 4 0.0 3 0 0 0 4 138 33 0.0 3 0 0 0 33 139 61 0.0 3 0 0 2 59 140 2 0.0 3 0 0 0 2 141 11 0.0 3 0 0 0 11 142 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 147 7 0.0 3 0 0 0 7 148 17 0.0 3 0 0 0 17 149 5 0.0 3 0 0 0 5 151 2 0.0 3 0 0 0 2 152 5 0.0 3 0 0 0 5 153 1 0.0 3 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 3 0.0 3 0 0 0 3 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 1 3 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 4 0.0 3 0 0 0 4 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 170 2 0.0 3 0 0 0 2 172 4 0.0 3 0 0 0 4 173 2 0.0 3 0 0 0 2 175 2 0.0 3 0 0 0 2 177 4 0.0 3 0 0 0 4 178 3 0.0 3 0 0 0 3 179 13 0.0 3 0 0 0 13 181 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 187 6 0.0 3 0 0 0 6 188 45 0.0 3 0 0 2 43 189 95 0.0 3 0 0 3 92 190 10 0.0 3 0 0 1 9 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 1 194 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 199 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 227 4 0.0 3 0 0 1 3 228 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 7,737 Reads with adapters: 16 (0.2%) Reads written (passing filters): 7,737 (100.0%) Total basepairs processed: 1,972,706 bp Total written (filtered): 1,972,651 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 16 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.8% C: 0.0% G: 62.5% T: 18.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 120.9 0 11 4 3 30.2 0 3 5 2 7.6 0 2