This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.41 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 344,904 (13.4%) Reads that were too short: 10,542 (0.4%) Reads written (passing filters): 334,362 (13.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 63,833,003 bp (8.2%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 344904 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 118 628.5 0 118 7 6990 628.5 0 6990 8 7382 628.5 0 7382 9 34 628.5 0 34 10 20 628.5 0 20 11 44 628.5 0 44 12 66 628.5 0 66 13 66 628.5 0 66 14 62 628.5 0 62 15 46 628.5 0 46 16 32 628.5 0 32 17 35 628.5 0 35 18 27 628.5 0 27 19 28 628.5 0 28 20 43 628.5 0 43 21 38 628.5 0 38 22 48 628.5 0 48 23 38 628.5 0 38 24 31 628.5 0 31 25 63 628.5 0 63 26 61 628.5 0 61 27 79 628.5 0 79 28 56 628.5 0 56 29 47 628.5 0 47 30 42 628.5 0 42 31 39 628.5 0 39 32 49 628.5 0 49 33 47 628.5 0 47 34 29 628.5 0 29 35 62 628.5 0 62 36 75 628.5 0 75 37 67 628.5 0 67 38 54 628.5 0 54 39 53 628.5 0 53 40 46 628.5 0 46 41 55 628.5 0 55 42 50 628.5 0 50 43 67 628.5 0 67 44 44 628.5 0 44 45 37 628.5 0 37 46 47 628.5 0 47 47 44 628.5 0 44 48 53 628.5 0 53 49 91 628.5 0 91 50 46 628.5 0 46 51 47 628.5 0 47 52 32 628.5 0 32 53 51 628.5 0 51 54 58 628.5 0 58 55 72 628.5 0 72 56 41 628.5 0 41 57 54 628.5 0 54 58 48 628.5 0 48 59 46 628.5 0 46 60 78 628.5 0 78 61 83 628.5 0 83 62 106 628.5 0 106 63 65 628.5 0 65 64 50 628.5 0 50 65 113 628.5 0 113 66 149 628.5 0 149 67 104 628.5 0 104 68 59 628.5 0 59 69 73 628.5 0 73 70 43 628.5 0 43 71 64 628.5 0 64 72 97 628.5 0 97 73 84 628.5 0 84 74 93 628.5 0 93 75 46 628.5 0 46 76 51 628.5 0 51 77 78 628.5 0 78 78 164 628.5 0 164 79 106 628.5 0 106 80 232 628.5 0 232 81 137 628.5 0 137 82 104 628.5 0 104 83 60 628.5 0 60 84 129 628.5 0 129 85 89 628.5 0 89 86 144 628.5 0 144 87 224 628.5 0 224 88 312 628.5 0 312 89 513 628.5 0 513 90 1022 628.5 0 1022 91 2127 628.5 0 2127 92 509 628.5 0 509 93 571 628.5 0 571 94 1193 628.5 0 1193 95 370 628.5 0 370 96 379 628.5 0 379 97 909 628.5 0 909 98 977 628.5 0 977 99 1165 628.5 0 1165 100 889 628.5 0 889 101 1256 628.5 0 1256 102 1969 628.5 0 1969 103 2303 628.5 0 2303 104 39203 628.5 0 39203 105 63089 628.5 0 63089 106 133060 628.5 0 133060 107 462 628.5 0 462 108 41 628.5 0 41 109 38 628.5 0 38 110 50 628.5 0 50 111 65 628.5 0 65 112 49 628.5 0 49 113 58 628.5 0 58 114 108 628.5 0 108 115 75 628.5 0 75 116 73 628.5 0 73 117 42 628.5 0 42 118 33 628.5 0 33 119 55 628.5 0 55 120 55 628.5 0 55 121 51 628.5 0 51 122 46 628.5 0 46 123 52 628.5 0 52 124 71 628.5 0 71 125 133 628.5 0 133 126 163 628.5 0 163 127 56 628.5 0 56 128 45 628.5 0 45 129 60 628.5 0 60 130 61 628.5 0 61 131 43 628.5 0 43 132 113 628.5 0 113 133 52 628.5 0 52 134 41 628.5 0 41 135 38 628.5 0 38 136 45 628.5 0 45 137 49 628.5 0 49 138 67 628.5 0 67 139 77 628.5 0 77 140 79 628.5 0 79 141 83 628.5 0 83 142 163 628.5 0 163 143 50 628.5 0 50 144 67 628.5 0 67 145 61 628.5 0 61 146 94 628.5 0 94 147 203 628.5 0 203 148 105 628.5 0 105 149 107 628.5 0 107 150 71 628.5 0 71 151 120 628.5 0 120 152 224 628.5 0 224 153 322 628.5 0 322 154 288 628.5 0 288 155 332 628.5 0 332 156 356 628.5 0 356 157 902 628.5 0 902 158 1184 628.5 0 1184 159 20156 628.5 0 20156 160 10973 628.5 0 10973 161 20202 628.5 0 20202 162 158 628.5 0 158 163 41 628.5 0 41 164 56 628.5 0 56 165 57 628.5 0 57 166 39 628.5 0 39 167 123 628.5 0 123 168 192 628.5 0 192 169 272 628.5 0 272 170 44 628.5 0 44 171 61 628.5 0 61 172 90 628.5 0 90 173 110 628.5 0 110 174 81 628.5 0 81 175 43 628.5 0 43 176 47 628.5 0 47 177 47 628.5 0 47 178 44 628.5 0 44 179 39 628.5 0 39 180 97 628.5 0 97 181 58 628.5 0 58 182 44 628.5 0 44 183 47 628.5 0 47 184 179 628.5 0 179 185 98 628.5 0 98 186 192 628.5 0 192 187 329 628.5 0 329 188 118 628.5 0 118 189 401 628.5 0 401 190 76 628.5 0 76 191 41 628.5 0 41 192 46 628.5 0 46 193 47 628.5 0 47 194 38 628.5 0 38 195 46 628.5 0 46 196 75 628.5 0 75 197 57 628.5 0 57 198 73 628.5 0 73 199 84 628.5 0 84 200 251 628.5 0 251 201 171 628.5 0 171 202 405 628.5 0 405 203 38 628.5 0 38 204 53 628.5 0 53 205 56 628.5 0 56 206 48 628.5 0 48 207 66 628.5 0 66 208 62 628.5 0 62 209 65 628.5 0 65 210 44 628.5 0 44 211 40 628.5 0 40 212 57 628.5 0 57 213 48 628.5 0 48 214 37 628.5 0 37 215 55 628.5 0 55 216 60 628.5 0 60 217 52 628.5 0 52 218 61 628.5 0 61 219 74 628.5 0 74 220 93 628.5 0 93 221 70 628.5 0 70 222 39 628.5 0 39 223 33 628.5 0 33 224 45 628.5 0 45 225 55 628.5 0 55 226 71 628.5 0 71 227 83 628.5 0 83 228 90 628.5 0 90 229 82 628.5 0 82 230 86 628.5 0 86 231 49 628.5 0 49 232 51 628.5 0 51 233 63 628.5 0 63 234 54 628.5 0 54 235 48 628.5 0 48 236 50 628.5 0 50 237 56 628.5 0 56 238 108 628.5 0 108 239 109 628.5 0 109 240 586 628.5 0 586 241 474 628.5 0 474 242 870 628.5 0 870 243 298 628.5 0 298 244 53 628.5 0 53 245 41 628.5 0 41 246 46 628.5 0 46 247 43 628.5 0 43 248 67 628.5 0 67 249 82 628.5 0 82 250 55 628.5 0 55 251 61 628.5 0 61 252 58 628.5 0 58 253 55 628.5 0 55 254 75 628.5 0 75 255 69 628.5 0 69 256 82 628.5 0 82 257 78 628.5 0 78 258 60 628.5 0 60 259 58 628.5 0 58 260 57 628.5 0 57 261 59 628.5 0 59 262 59 628.5 0 59 263 62 628.5 0 62 264 42 628.5 0 42 265 47 628.5 0 47 266 66 628.5 0 66 267 84 628.5 0 84 268 68 628.5 0 68 269 73 628.5 0 73 270 81 628.5 0 81 271 61 628.5 0 61 272 69 628.5 0 69 273 67 628.5 0 67 274 118 628.5 0 118 275 117 628.5 0 117 276 97 628.5 0 97 277 122 628.5 0 122 278 393 628.5 0 393 279 642 628.5 0 642 280 141 628.5 0 141 281 189 628.5 0 189 282 260 628.5 0 260 283 211 628.5 0 211 284 84 628.5 0 84 285 61 628.5 0 61 286 44 628.5 0 44 287 62 628.5 0 62 288 74 628.5 0 74 289 54 628.5 0 54 290 71 628.5 0 71 291 83 628.5 0 83 292 57 628.5 0 57 293 79 628.5 0 79 294 114 628.5 0 114 295 97 628.5 0 97 296 80 628.5 0 80 297 61 628.5 0 61 298 71 628.5 0 71 299 77 628.5 0 77 300 129 628.5 0 129 301 192 628.5 0 192 Finished in 13.93 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 334,362 Reads with adapters: 35,155 (10.5%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 35,133 (10.5%) Total basepairs processed: 63,833,003 bp Total written (filtered): 6,650,604 bp (10.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35155 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 95 0.0 3 0 94 0 1 20 7396 0.0 4 7291 102 3 21 17 0.0 4 2 15 22 1 0.0 4 0 0 1 23 7 0.0 4 3 0 0 4 24 1 0.0 4 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 29 6 0.0 4 0 0 0 0 6 30 26599 0.0 4 0 0 0 0 26599 33 24 0.0 4 0 0 0 0 24 40 1 0.0 4 0 1 52 1 0.0 4 0 1 57 1 0.0 4 1 69 2 0.0 4 0 0 0 0 2 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 1 81 1 0.0 4 1 109 2 0.0 4 0 0 0 0 2 112 1 0.0 4 0 0 0 0 1 122 1 0.0 4 1 125 3 0.0 4 0 0 0 0 3 126 11 0.0 4 0 0 0 0 11 127 957 0.0 4 0 0 0 0 957 128 2 0.0 4 0 0 0 0 2 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 1 161 1 0.0 4 1 162 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 1 185 4 0.0 4 0 0 0 0 4 186 1 0.0 4 0 0 0 0 1 188 3 0.0 4 0 0 0 0 3 196 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.00 s (28 us/read; 2.11 M reads/minute). === Summary === Total reads processed: 35,133 Reads with adapters: 1,372 (3.9%) Reads written (passing filters): 35,133 (100.0%) Total basepairs processed: 6,650,604 bp Total written (filtered): 6,637,580 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1372 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 21.6% C: 2.3% G: 74.9% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 76 549.0 0 76 4 216 137.2 0 216 5 9 34.3 0 9 9 3 0.1 0 0 3 10 36 0.0 1 1 35 11 1029 0.0 1 5 1024 12 1 0.0 1 0 1 63 1 0.0 1 1 106 1 0.0 1 1