This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.25 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 155,907 (6.1%) Reads that were too short: 27,381 (1.1%) Reads written (passing filters): 128,526 (5.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 28,125,831 bp (3.6%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 155907 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 782 628.5 0 782 7 24972 628.5 0 24972 8 19324 628.5 0 19324 9 62 628.5 0 62 10 52 628.5 0 52 11 84 628.5 0 84 12 63 628.5 0 63 13 66 628.5 0 66 14 57 628.5 0 57 15 45 628.5 0 45 16 46 628.5 0 46 17 68 628.5 0 68 18 42 628.5 0 42 19 45 628.5 0 45 20 88 628.5 0 88 21 60 628.5 0 60 22 60 628.5 0 60 23 63 628.5 0 63 24 70 628.5 0 70 25 57 628.5 0 57 26 56 628.5 0 56 27 83 628.5 0 83 28 98 628.5 0 98 29 637 628.5 0 637 30 138 628.5 0 138 31 942 628.5 0 942 32 54 628.5 0 54 33 37 628.5 0 37 34 62 628.5 0 62 35 68 628.5 0 68 36 103 628.5 0 103 37 104 628.5 0 104 38 59 628.5 0 59 39 97 628.5 0 97 40 71 628.5 0 71 41 39 628.5 0 39 42 70 628.5 0 70 43 67 628.5 0 67 44 82 628.5 0 82 45 71 628.5 0 71 46 84 628.5 0 84 47 61 628.5 0 61 48 80 628.5 0 80 49 78 628.5 0 78 50 72 628.5 0 72 51 79 628.5 0 79 52 64 628.5 0 64 53 79 628.5 0 79 54 85 628.5 0 85 55 72 628.5 0 72 56 74 628.5 0 74 57 81 628.5 0 81 58 66 628.5 0 66 59 86 628.5 0 86 60 76 628.5 0 76 61 75 628.5 0 75 62 90 628.5 0 90 63 71 628.5 0 71 64 76 628.5 0 76 65 91 628.5 0 91 66 85 628.5 0 85 67 97 628.5 0 97 68 107 628.5 0 107 69 115 628.5 0 115 70 994 628.5 0 994 71 387 628.5 0 387 72 539 628.5 0 539 73 81 628.5 0 81 74 85 628.5 0 85 75 56 628.5 0 56 76 67 628.5 0 67 77 75 628.5 0 75 78 89 628.5 0 89 79 115 628.5 0 115 80 83 628.5 0 83 81 127 628.5 0 127 82 202 628.5 0 202 83 176 628.5 0 176 84 78 628.5 0 78 85 71 628.5 0 71 86 78 628.5 0 78 87 67 628.5 0 67 88 52 628.5 0 52 89 48 628.5 0 48 90 56 628.5 0 56 91 68 628.5 0 68 92 57 628.5 0 57 93 68 628.5 0 68 94 77 628.5 0 77 95 75 628.5 0 75 96 58 628.5 0 58 97 127 628.5 0 127 98 117 628.5 0 117 99 94 628.5 0 94 100 92 628.5 0 92 101 122 628.5 0 122 102 98 628.5 0 98 103 81 628.5 0 81 104 74 628.5 0 74 105 103 628.5 0 103 106 74 628.5 0 74 107 107 628.5 0 107 108 112 628.5 0 112 109 178 628.5 0 178 110 252 628.5 0 252 111 487 628.5 0 487 112 211 628.5 0 211 113 199 628.5 0 199 114 262 628.5 0 262 115 159 628.5 0 159 116 174 628.5 0 174 117 278 628.5 0 278 118 270 628.5 0 270 119 355 628.5 0 355 120 324 628.5 0 324 121 449 628.5 0 449 122 548 628.5 0 548 123 924 628.5 0 924 124 13487 628.5 0 13487 125 18205 628.5 0 18205 126 17297 628.5 0 17297 127 582 628.5 0 582 128 132 628.5 0 132 129 188 628.5 0 188 130 100 628.5 0 100 131 105 628.5 0 105 132 84 628.5 0 84 133 76 628.5 0 76 134 93 628.5 0 93 135 126 628.5 0 126 136 223 628.5 0 223 137 174 628.5 0 174 138 283 628.5 0 283 139 232 628.5 0 232 140 563 628.5 0 563 141 213 628.5 0 213 142 213 628.5 0 213 143 94 628.5 0 94 144 125 628.5 0 125 145 242 628.5 0 242 146 146 628.5 0 146 147 143 628.5 0 143 148 222 628.5 0 222 149 2760 628.5 0 2760 150 2196 628.5 0 2196 151 3443 628.5 0 3443 152 114 628.5 0 114 153 69 628.5 0 69 154 84 628.5 0 84 155 87 628.5 0 87 156 92 628.5 0 92 157 101 628.5 0 101 158 101 628.5 0 101 159 84 628.5 0 84 160 78 628.5 0 78 161 80 628.5 0 80 162 142 628.5 0 142 163 228 628.5 0 228 164 244 628.5 0 244 165 167 628.5 0 167 166 73 628.5 0 73 167 71 628.5 0 71 168 64 628.5 0 64 169 89 628.5 0 89 170 71 628.5 0 71 171 86 628.5 0 86 172 68 628.5 0 68 173 64 628.5 0 64 174 73 628.5 0 73 175 70 628.5 0 70 176 63 628.5 0 63 177 70 628.5 0 70 178 64 628.5 0 64 179 82 628.5 0 82 180 91 628.5 0 91 181 224 628.5 0 224 182 192 628.5 0 192 183 289 628.5 0 289 184 103 628.5 0 103 185 122 628.5 0 122 186 102 628.5 0 102 187 69 628.5 0 69 188 131 628.5 0 131 189 299 628.5 0 299 190 500 628.5 0 500 191 55 628.5 0 55 192 65 628.5 0 65 193 50 628.5 0 50 194 75 628.5 0 75 195 83 628.5 0 83 196 78 628.5 0 78 197 79 628.5 0 79 198 114 628.5 0 114 199 76 628.5 0 76 200 56 628.5 0 56 201 59 628.5 0 59 202 63 628.5 0 63 203 125 628.5 0 125 204 143 628.5 0 143 205 193 628.5 0 193 206 236 628.5 0 236 207 120 628.5 0 120 208 209 628.5 0 209 209 250 628.5 0 250 210 105 628.5 0 105 211 88 628.5 0 88 212 105 628.5 0 105 213 116 628.5 0 116 214 104 628.5 0 104 215 85 628.5 0 85 216 96 628.5 0 96 217 85 628.5 0 85 218 90 628.5 0 90 219 114 628.5 0 114 220 177 628.5 0 177 221 225 628.5 0 225 222 249 628.5 0 249 223 1048 628.5 0 1048 224 1017 628.5 0 1017 225 4932 628.5 0 4932 226 3061 628.5 0 3061 227 7143 628.5 0 7143 228 97 628.5 0 97 229 86 628.5 0 86 230 91 628.5 0 91 231 81 628.5 0 81 232 94 628.5 0 94 233 97 628.5 0 97 234 117 628.5 0 117 235 116 628.5 0 116 236 137 628.5 0 137 237 143 628.5 0 143 238 99 628.5 0 99 239 84 628.5 0 84 240 106 628.5 0 106 241 97 628.5 0 97 242 107 628.5 0 107 243 173 628.5 0 173 244 217 628.5 0 217 245 230 628.5 0 230 246 198 628.5 0 198 247 68 628.5 0 68 248 51 628.5 0 51 249 64 628.5 0 64 250 65 628.5 0 65 251 82 628.5 0 82 252 78 628.5 0 78 253 94 628.5 0 94 254 109 628.5 0 109 255 106 628.5 0 106 256 157 628.5 0 157 257 147 628.5 0 147 258 143 628.5 0 143 259 111 628.5 0 111 260 91 628.5 0 91 261 77 628.5 0 77 262 101 628.5 0 101 263 143 628.5 0 143 264 136 628.5 0 136 265 219 628.5 0 219 266 156 628.5 0 156 267 143 628.5 0 143 268 168 628.5 0 168 269 146 628.5 0 146 270 70 628.5 0 70 271 51 628.5 0 51 272 82 628.5 0 82 273 79 628.5 0 79 274 95 628.5 0 95 275 92 628.5 0 92 276 78 628.5 0 78 277 80 628.5 0 80 278 66 628.5 0 66 279 79 628.5 0 79 280 80 628.5 0 80 281 80 628.5 0 80 282 59 628.5 0 59 283 66 628.5 0 66 284 53 628.5 0 53 285 48 628.5 0 48 286 69 628.5 0 69 287 59 628.5 0 59 288 60 628.5 0 60 289 67 628.5 0 67 290 51 628.5 0 51 291 54 628.5 0 54 292 45 628.5 0 45 293 69 628.5 0 69 294 57 628.5 0 57 295 86 628.5 0 86 296 48 628.5 0 48 297 63 628.5 0 63 298 69 628.5 0 69 299 92 628.5 0 92 300 70 628.5 0 70 301 60 628.5 0 60 Finished in 5.35 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 128,526 Reads with adapters: 26,593 (20.7%) Reads that were too short: 253 (0.2%) Reads written (passing filters): 26,340 (20.5%) Total basepairs processed: 28,125,831 bp Total written (filtered): 7,040,102 bp (25.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26593 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 11 0.0 3 0 0 5 6 16 92 0.0 3 0 40 35 17 17 1881 0.0 3 1393 381 66 41 18 22607 0.0 3 20243 1821 414 129 19 72 0.0 3 2 46 16 8 20 165 0.0 3 0 1 90 74 21 58 0.0 3 0 0 0 58 22 3 0.0 3 0 0 0 3 23 5 0.0 3 0 0 0 5 24 3 0.0 3 0 0 0 3 25 2 0.0 3 0 0 0 2 26 13 0.0 3 0 0 1 12 28 1 0.0 3 0 0 0 1 29 32 0.0 3 0 0 0 32 32 4 0.0 3 0 0 0 4 33 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 536 0.0 3 0 0 9 527 43 3 0.0 3 0 0 0 3 44 3 0.0 3 0 0 0 3 45 104 0.0 3 0 0 5 99 46 2 0.0 3 0 0 0 2 49 2 0.0 3 0 0 0 2 52 2 0.0 3 0 0 0 2 53 2 0.0 3 1 0 0 1 54 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 56 1 0.0 3 0 0 0 1 58 4 0.0 3 0 0 2 2 59 1 0.0 3 0 0 1 60 1 0.0 3 1 61 1 0.0 3 0 1 62 4 0.0 3 0 0 2 2 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 5 0.0 3 0 0 1 4 66 26 0.0 3 0 0 2 24 67 1 0.0 3 0 0 0 1 68 654 0.0 3 0 0 0 654 69 8 0.0 3 0 0 1 7 71 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 3 0.0 3 0 0 0 3 85 3 0.0 3 0 0 0 3 93 32 0.0 3 0 0 0 32 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 102 25 0.0 3 0 0 1 24 103 2 0.0 3 0 0 0 2 105 4 0.0 3 0 0 0 4 110 7 0.0 3 0 0 0 7 111 6 0.0 3 0 0 0 6 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 115 6 0.0 3 0 0 0 6 116 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 135 2 0.0 3 0 0 1 1 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 13 0.0 3 0 0 0 13 143 34 0.0 3 0 0 2 32 144 31 0.0 3 0 0 2 29 145 6 0.0 3 0 0 0 6 146 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 6 0.0 3 0 0 0 6 155 3 0.0 3 0 0 0 3 156 1 0.0 3 0 0 0 1 157 3 0.0 3 0 0 0 3 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 195 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 1 3 282 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 292 2 0.0 3 0 0 0 2 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.91 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 26,340 Reads with adapters: 143 (0.5%) Reads written (passing filters): 26,340 (100.0%) Total basepairs processed: 7,040,102 bp Total written (filtered): 7,039,621 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 143 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.2% C: 9.8% G: 37.8% T: 6.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 99 411.6 0 99 4 42 102.9 0 42 5 1 25.7 0 1 11 1 0.0 1 0 1