This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.66 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 156,639 (6.1%) Reads that were too short: 28,045 (1.1%) Reads written (passing filters): 128,594 (5.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 28,361,690 bp (3.7%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 156639 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 583 628.5 0 583 7 24555 628.5 0 24555 8 22679 628.5 0 22679 9 41 628.5 0 41 10 24 628.5 0 24 11 66 628.5 0 66 12 43 628.5 0 43 13 39 628.5 0 39 14 32 628.5 0 32 15 29 628.5 0 29 16 32 628.5 0 32 17 54 628.5 0 54 18 33 628.5 0 33 19 34 628.5 0 34 20 52 628.5 0 52 21 49 628.5 0 49 22 41 628.5 0 41 23 41 628.5 0 41 24 44 628.5 0 44 25 31 628.5 0 31 26 54 628.5 0 54 27 76 628.5 0 76 28 80 628.5 0 80 29 619 628.5 0 619 30 89 628.5 0 89 31 873 628.5 0 873 32 33 628.5 0 33 33 32 628.5 0 32 34 36 628.5 0 36 35 49 628.5 0 49 36 62 628.5 0 62 37 55 628.5 0 55 38 57 628.5 0 57 39 60 628.5 0 60 40 52 628.5 0 52 41 30 628.5 0 30 42 38 628.5 0 38 43 37 628.5 0 37 44 50 628.5 0 50 45 44 628.5 0 44 46 52 628.5 0 52 47 55 628.5 0 55 48 59 628.5 0 59 49 51 628.5 0 51 50 40 628.5 0 40 51 34 628.5 0 34 52 38 628.5 0 38 53 42 628.5 0 42 54 49 628.5 0 49 55 33 628.5 0 33 56 43 628.5 0 43 57 48 628.5 0 48 58 42 628.5 0 42 59 62 628.5 0 62 60 55 628.5 0 55 61 53 628.5 0 53 62 47 628.5 0 47 63 40 628.5 0 40 64 60 628.5 0 60 65 62 628.5 0 62 66 55 628.5 0 55 67 76 628.5 0 76 68 76 628.5 0 76 69 99 628.5 0 99 70 1072 628.5 0 1072 71 404 628.5 0 404 72 540 628.5 0 540 73 43 628.5 0 43 74 39 628.5 0 39 75 38 628.5 0 38 76 34 628.5 0 34 77 41 628.5 0 41 78 43 628.5 0 43 79 61 628.5 0 61 80 55 628.5 0 55 81 97 628.5 0 97 82 140 628.5 0 140 83 96 628.5 0 96 84 51 628.5 0 51 85 56 628.5 0 56 86 55 628.5 0 55 87 39 628.5 0 39 88 39 628.5 0 39 89 34 628.5 0 34 90 46 628.5 0 46 91 43 628.5 0 43 92 48 628.5 0 48 93 53 628.5 0 53 94 42 628.5 0 42 95 48 628.5 0 48 96 41 628.5 0 41 97 79 628.5 0 79 98 78 628.5 0 78 99 80 628.5 0 80 100 77 628.5 0 77 101 69 628.5 0 69 102 61 628.5 0 61 103 57 628.5 0 57 104 53 628.5 0 53 105 61 628.5 0 61 106 58 628.5 0 58 107 100 628.5 0 100 108 91 628.5 0 91 109 149 628.5 0 149 110 228 628.5 0 228 111 487 628.5 0 487 112 159 628.5 0 159 113 162 628.5 0 162 114 282 628.5 0 282 115 151 628.5 0 151 116 135 628.5 0 135 117 262 628.5 0 262 118 233 628.5 0 233 119 296 628.5 0 296 120 241 628.5 0 241 121 344 628.5 0 344 122 433 628.5 0 433 123 660 628.5 0 660 124 13845 628.5 0 13845 125 19198 628.5 0 19198 126 18551 628.5 0 18551 127 546 628.5 0 546 128 81 628.5 0 81 129 85 628.5 0 85 130 50 628.5 0 50 131 48 628.5 0 48 132 35 628.5 0 35 133 52 628.5 0 52 134 50 628.5 0 50 135 103 628.5 0 103 136 131 628.5 0 131 137 81 628.5 0 81 138 142 628.5 0 142 139 186 628.5 0 186 140 522 628.5 0 522 141 180 628.5 0 180 142 153 628.5 0 153 143 69 628.5 0 69 144 86 628.5 0 86 145 181 628.5 0 181 146 113 628.5 0 113 147 93 628.5 0 93 148 154 628.5 0 154 149 3055 628.5 0 3055 150 2314 628.5 0 2314 151 3893 628.5 0 3893 152 94 628.5 0 94 153 42 628.5 0 42 154 42 628.5 0 42 155 45 628.5 0 45 156 49 628.5 0 49 157 56 628.5 0 56 158 60 628.5 0 60 159 48 628.5 0 48 160 39 628.5 0 39 161 40 628.5 0 40 162 59 628.5 0 59 163 215 628.5 0 215 164 214 628.5 0 214 165 169 628.5 0 169 166 41 628.5 0 41 167 34 628.5 0 34 168 43 628.5 0 43 169 45 628.5 0 45 170 30 628.5 0 30 171 64 628.5 0 64 172 38 628.5 0 38 173 41 628.5 0 41 174 43 628.5 0 43 175 45 628.5 0 45 176 48 628.5 0 48 177 49 628.5 0 49 178 44 628.5 0 44 179 53 628.5 0 53 180 55 628.5 0 55 181 143 628.5 0 143 182 110 628.5 0 110 183 190 628.5 0 190 184 48 628.5 0 48 185 64 628.5 0 64 186 48 628.5 0 48 187 51 628.5 0 51 188 118 628.5 0 118 189 319 628.5 0 319 190 573 628.5 0 573 191 38 628.5 0 38 192 26 628.5 0 26 193 35 628.5 0 35 194 61 628.5 0 61 195 40 628.5 0 40 196 62 628.5 0 62 197 72 628.5 0 72 198 75 628.5 0 75 199 44 628.5 0 44 200 27 628.5 0 27 201 25 628.5 0 25 202 34 628.5 0 34 203 40 628.5 0 40 204 64 628.5 0 64 205 60 628.5 0 60 206 75 628.5 0 75 207 84 628.5 0 84 208 110 628.5 0 110 209 101 628.5 0 101 210 87 628.5 0 87 211 72 628.5 0 72 212 53 628.5 0 53 213 103 628.5 0 103 214 64 628.5 0 64 215 63 628.5 0 63 216 63 628.5 0 63 217 52 628.5 0 52 218 63 628.5 0 63 219 83 628.5 0 83 220 133 628.5 0 133 221 202 628.5 0 202 222 241 628.5 0 241 223 1112 628.5 0 1112 224 922 628.5 0 922 225 6118 628.5 0 6118 226 3988 628.5 0 3988 227 9957 628.5 0 9957 228 92 628.5 0 92 229 61 628.5 0 61 230 56 628.5 0 56 231 43 628.5 0 43 232 54 628.5 0 54 233 43 628.5 0 43 234 59 628.5 0 59 235 67 628.5 0 67 236 85 628.5 0 85 237 63 628.5 0 63 238 52 628.5 0 52 239 54 628.5 0 54 240 58 628.5 0 58 241 63 628.5 0 63 242 64 628.5 0 64 243 80 628.5 0 80 244 92 628.5 0 92 245 104 628.5 0 104 246 58 628.5 0 58 247 42 628.5 0 42 248 41 628.5 0 41 249 42 628.5 0 42 250 44 628.5 0 44 251 44 628.5 0 44 252 43 628.5 0 43 253 68 628.5 0 68 254 58 628.5 0 58 255 64 628.5 0 64 256 84 628.5 0 84 257 71 628.5 0 71 258 68 628.5 0 68 259 54 628.5 0 54 260 58 628.5 0 58 261 48 628.5 0 48 262 40 628.5 0 40 263 64 628.5 0 64 264 66 628.5 0 66 265 97 628.5 0 97 266 93 628.5 0 93 267 73 628.5 0 73 268 90 628.5 0 90 269 59 628.5 0 59 270 44 628.5 0 44 271 47 628.5 0 47 272 33 628.5 0 33 273 41 628.5 0 41 274 40 628.5 0 40 275 51 628.5 0 51 276 51 628.5 0 51 277 49 628.5 0 49 278 42 628.5 0 42 279 45 628.5 0 45 280 59 628.5 0 59 281 77 628.5 0 77 282 56 628.5 0 56 283 57 628.5 0 57 284 44 628.5 0 44 285 53 628.5 0 53 286 45 628.5 0 45 287 40 628.5 0 40 288 33 628.5 0 33 289 33 628.5 0 33 290 31 628.5 0 31 291 31 628.5 0 31 292 35 628.5 0 35 293 41 628.5 0 41 294 38 628.5 0 38 295 64 628.5 0 64 296 48 628.5 0 48 297 38 628.5 0 38 298 42 628.5 0 42 299 40 628.5 0 40 300 58 628.5 0 58 301 36 628.5 0 36 Finished in 5.89 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 128,594 Reads with adapters: 23,934 (18.6%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 23,914 (18.6%) Total basepairs processed: 28,361,690 bp Total written (filtered): 6,446,423 bp (22.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23934 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 29 0.0 3 0 22 7 20 2121 0.0 4 1844 271 4 2 21 21103 0.0 4 20800 293 9 1 22 41 0.0 4 6 34 1 23 8 0.0 4 1 1 3 0 3 24 12 0.0 4 1 0 0 11 25 16 0.0 4 1 0 0 0 15 26 1 0.0 4 0 0 0 0 1 27 8 0.0 4 0 0 0 0 8 40 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 65 1 0.0 4 1 66 3 0.0 4 2 0 1 67 1 0.0 4 1 68 1 0.0 4 1 71 1 0.0 4 0 0 0 0 1 79 1 0.0 4 1 112 3 0.0 4 1 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 92 0.0 4 0 0 0 0 92 128 465 0.0 4 0 0 0 0 465 129 1 0.0 4 0 0 0 0 1 131 2 0.0 4 0 0 0 0 2 140 2 0.0 4 0 0 0 0 2 143 2 0.0 4 0 0 0 0 2 163 1 0.0 4 0 0 0 0 1 164 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 195 1 0.0 4 1 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.85 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 23,914 Reads with adapters: 2,975 (12.4%) Reads written (passing filters): 23,914 (100.0%) Total basepairs processed: 6,446,423 bp Total written (filtered): 6,405,212 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2975 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 4.9% G: 92.7% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 373.7 0 6 4 2 93.4 0 2 5 2 23.4 0 2 6 2 5.8 0 2 9 923 0.1 0 35 888 10 1454 0.0 1 47 1407 11 526 0.0 1 1 525 12 3 0.0 1 0 3 13 1 0.0 1 0 1 150 1 0.0 1 0 1 170 1 0.0 1 1 185 1 0.0 1 0 1 189 1 0.0 1 0 1 223 47 0.0 1 46 1 257 1 0.0 1 1 259 3 0.0 1 3 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.