This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 127.83 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 95,486 (3.2%) Reads that were too short: 16,323 (0.6%) Reads written (passing filters): 79,163 (2.7%) Total basepairs processed: 887,907,860 bp Total written (filtered): 19,050,869 bp (2.1%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 95486 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1276 720.2 0 1276 7 6029 720.2 0 6029 8 4661 720.2 0 4661 9 59 720.2 0 59 10 353 720.2 0 353 11 70 720.2 0 70 12 39 720.2 0 39 13 20 720.2 0 20 14 32 720.2 0 32 15 15 720.2 0 15 16 14 720.2 0 14 17 27 720.2 0 27 18 43 720.2 0 43 19 30 720.2 0 30 20 22 720.2 0 22 21 40 720.2 0 40 22 43 720.2 0 43 23 22 720.2 0 22 24 27 720.2 0 27 25 19 720.2 0 19 26 29 720.2 0 29 27 35 720.2 0 35 28 72 720.2 0 72 29 24 720.2 0 24 30 35 720.2 0 35 31 24 720.2 0 24 32 21 720.2 0 21 33 22 720.2 0 22 34 22 720.2 0 22 35 23 720.2 0 23 36 39 720.2 0 39 37 41 720.2 0 41 38 89 720.2 0 89 39 33 720.2 0 33 40 66 720.2 0 66 41 69 720.2 0 69 42 49 720.2 0 49 43 118 720.2 0 118 44 60 720.2 0 60 45 30 720.2 0 30 46 18 720.2 0 18 47 41 720.2 0 41 48 93 720.2 0 93 49 136 720.2 0 136 50 188 720.2 0 188 51 349 720.2 0 349 52 365 720.2 0 365 53 606 720.2 0 606 54 896 720.2 0 896 55 16194 720.2 0 16194 56 5075 720.2 0 5075 57 27952 720.2 0 27952 58 129 720.2 0 129 59 23 720.2 0 23 60 27 720.2 0 27 61 29 720.2 0 29 62 16 720.2 0 16 63 44 720.2 0 44 64 23 720.2 0 23 65 34 720.2 0 34 66 22 720.2 0 22 67 32 720.2 0 32 68 23 720.2 0 23 69 40 720.2 0 40 70 20 720.2 0 20 71 23 720.2 0 23 72 28 720.2 0 28 73 32 720.2 0 32 74 51 720.2 0 51 75 35 720.2 0 35 76 86 720.2 0 86 77 54 720.2 0 54 78 113 720.2 0 113 79 19 720.2 0 19 80 33 720.2 0 33 81 47 720.2 0 47 82 41 720.2 0 41 83 58 720.2 0 58 84 97 720.2 0 97 85 778 720.2 0 778 86 432 720.2 0 432 87 1524 720.2 0 1524 88 35 720.2 0 35 89 44 720.2 0 44 90 34 720.2 0 34 91 30 720.2 0 30 92 100 720.2 0 100 93 38 720.2 0 38 94 32 720.2 0 32 95 35 720.2 0 35 96 339 720.2 0 339 97 45 720.2 0 45 98 34 720.2 0 34 99 28 720.2 0 28 100 52 720.2 0 52 101 49 720.2 0 49 102 323 720.2 0 323 103 94 720.2 0 94 104 377 720.2 0 377 105 52 720.2 0 52 106 36 720.2 0 36 107 34 720.2 0 34 108 33 720.2 0 33 109 38 720.2 0 38 110 39 720.2 0 39 111 34 720.2 0 34 112 31 720.2 0 31 113 25 720.2 0 25 114 26 720.2 0 26 115 21 720.2 0 21 116 15 720.2 0 15 117 34 720.2 0 34 118 130 720.2 0 130 119 220 720.2 0 220 120 118 720.2 0 118 121 31 720.2 0 31 122 27 720.2 0 27 123 36 720.2 0 36 124 68 720.2 0 68 125 24 720.2 0 24 126 84 720.2 0 84 127 41 720.2 0 41 128 50 720.2 0 50 129 118 720.2 0 118 130 71 720.2 0 71 131 43 720.2 0 43 132 68 720.2 0 68 133 91 720.2 0 91 134 62 720.2 0 62 135 42 720.2 0 42 136 42 720.2 0 42 137 122 720.2 0 122 138 288 720.2 0 288 139 307 720.2 0 307 140 110 720.2 0 110 141 61 720.2 0 61 142 79 720.2 0 79 143 28 720.2 0 28 144 47 720.2 0 47 145 32 720.2 0 32 146 50 720.2 0 50 147 114 720.2 0 114 148 110 720.2 0 110 149 75 720.2 0 75 150 36 720.2 0 36 151 65 720.2 0 65 152 65 720.2 0 65 153 66 720.2 0 66 154 49 720.2 0 49 155 60 720.2 0 60 156 71 720.2 0 71 157 157 720.2 0 157 158 197 720.2 0 197 159 152 720.2 0 152 160 25 720.2 0 25 161 54 720.2 0 54 162 41 720.2 0 41 163 79 720.2 0 79 164 89 720.2 0 89 165 160 720.2 0 160 166 91 720.2 0 91 167 119 720.2 0 119 168 149 720.2 0 149 169 140 720.2 0 140 170 71 720.2 0 71 171 55 720.2 0 55 172 43 720.2 0 43 173 39 720.2 0 39 174 47 720.2 0 47 175 43 720.2 0 43 176 65 720.2 0 65 177 36 720.2 0 36 178 48 720.2 0 48 179 35 720.2 0 35 180 40 720.2 0 40 181 55 720.2 0 55 182 76 720.2 0 76 183 108 720.2 0 108 184 109 720.2 0 109 185 54 720.2 0 54 186 66 720.2 0 66 187 260 720.2 0 260 188 387 720.2 0 387 189 317 720.2 0 317 190 246 720.2 0 246 191 68 720.2 0 68 192 145 720.2 0 145 193 26 720.2 0 26 194 41 720.2 0 41 195 43 720.2 0 43 196 31 720.2 0 31 197 36 720.2 0 36 198 37 720.2 0 37 199 85 720.2 0 85 200 62 720.2 0 62 201 60 720.2 0 60 202 33 720.2 0 33 203 40 720.2 0 40 204 40 720.2 0 40 205 44 720.2 0 44 206 62 720.2 0 62 207 77 720.2 0 77 208 73 720.2 0 73 209 125 720.2 0 125 210 31 720.2 0 31 211 34 720.2 0 34 212 44 720.2 0 44 213 40 720.2 0 40 214 78 720.2 0 78 215 76 720.2 0 76 216 54 720.2 0 54 217 40 720.2 0 40 218 51 720.2 0 51 219 367 720.2 0 367 220 251 720.2 0 251 221 481 720.2 0 481 222 29 720.2 0 29 223 44 720.2 0 44 224 84 720.2 0 84 225 126 720.2 0 126 226 1207 720.2 0 1207 227 23 720.2 0 23 228 53 720.2 0 53 229 31 720.2 0 31 230 35 720.2 0 35 231 40 720.2 0 40 232 40 720.2 0 40 233 48 720.2 0 48 234 114 720.2 0 114 235 204 720.2 0 204 236 63 720.2 0 63 237 26 720.2 0 26 238 76 720.2 0 76 239 129 720.2 0 129 240 183 720.2 0 183 241 115 720.2 0 115 242 56 720.2 0 56 243 67 720.2 0 67 244 30 720.2 0 30 245 35 720.2 0 35 246 65 720.2 0 65 247 61 720.2 0 61 248 59 720.2 0 59 249 53 720.2 0 53 250 152 720.2 0 152 251 113 720.2 0 113 252 88 720.2 0 88 253 32 720.2 0 32 254 21 720.2 0 21 255 21 720.2 0 21 256 43 720.2 0 43 257 51 720.2 0 51 258 98 720.2 0 98 259 152 720.2 0 152 260 289 720.2 0 289 261 380 720.2 0 380 262 188 720.2 0 188 263 54 720.2 0 54 264 43 720.2 0 43 265 38 720.2 0 38 266 54 720.2 0 54 267 81 720.2 0 81 268 62 720.2 0 62 269 92 720.2 0 92 270 65 720.2 0 65 271 48 720.2 0 48 272 51 720.2 0 51 273 53 720.2 0 53 274 110 720.2 0 110 275 90 720.2 0 90 276 301 720.2 0 301 277 188 720.2 0 188 278 724 720.2 0 724 279 325 720.2 0 325 280 522 720.2 0 522 281 160 720.2 0 160 282 153 720.2 0 153 283 146 720.2 0 146 284 104 720.2 0 104 285 82 720.2 0 82 286 69 720.2 0 69 287 294 720.2 0 294 288 520 720.2 0 520 289 281 720.2 0 281 290 109 720.2 0 109 291 123 720.2 0 123 292 149 720.2 0 149 293 218 720.2 0 218 294 129 720.2 0 129 295 145 720.2 0 145 296 191 720.2 0 191 297 173 720.2 0 173 298 829 720.2 0 829 299 1229 720.2 0 1229 300 1433 720.2 0 1433 301 120 720.2 0 120 Finished in 4.70 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 79,163 Reads with adapters: 6,224 (7.9%) Reads that were too short: 83 (0.1%) Reads written (passing filters): 6,141 (7.8%) Total basepairs processed: 19,050,869 bp Total written (filtered): 1,684,018 bp (8.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6224 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 1 0.0 3 0 0 0 1 16 47 0.0 3 0 39 5 3 17 5887 0.0 3 5547 284 51 5 18 39 0.0 3 13 21 4 1 19 34 0.0 3 7 5 14 8 20 11 0.0 3 1 0 0 10 21 11 0.0 3 0 0 0 11 27 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 34 37 0.0 3 0 0 0 37 37 9 0.0 3 0 0 1 8 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 1 45 4 0.0 3 0 0 0 4 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 1 52 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 55 3 0.0 3 0 0 0 3 57 6 0.0 3 0 0 0 6 58 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 66 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 79 3 0.0 3 0 0 1 2 80 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 146 5 0.0 3 0 0 0 5 147 3 0.0 3 0 0 0 3 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 1 2 161 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 163 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 181 3 0.0 3 0 0 0 3 182 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 231 2 0.0 3 0 0 0 2 232 2 0.0 3 0 0 0 2 233 4 0.0 3 0 0 0 4 234 1 0.0 3 0 0 0 1 235 5 0.0 3 0 0 1 4 236 1 0.0 3 0 0 0 1 240 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 6,141 Reads with adapters: 72 (1.2%) Reads written (passing filters): 6,141 (100.0%) Total basepairs processed: 1,684,018 bp Total written (filtered): 1,683,703 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 72 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.2% C: 50.0% G: 16.7% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 63 96.0 0 63 4 8 24.0 0 8 94 1 0.0 2 0 0 1