This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 129.79 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 100,957 (3.4%) Reads that were too short: 12,692 (0.4%) Reads written (passing filters): 88,265 (3.0%) Total basepairs processed: 887,907,860 bp Total written (filtered): 21,679,739 bp (2.4%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 100957 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1512 720.2 0 1512 7 4792 720.2 0 4792 8 6331 720.2 0 6331 9 55 720.2 0 55 10 24 720.2 0 24 11 22 720.2 0 22 12 36 720.2 0 36 13 14 720.2 0 14 14 14 720.2 0 14 15 12 720.2 0 12 16 23 720.2 0 23 17 15 720.2 0 15 18 51 720.2 0 51 19 31 720.2 0 31 20 21 720.2 0 21 21 33 720.2 0 33 22 16 720.2 0 16 23 22 720.2 0 22 24 27 720.2 0 27 25 23 720.2 0 23 26 15 720.2 0 15 27 25 720.2 0 25 28 97 720.2 0 97 29 20 720.2 0 20 30 23 720.2 0 23 31 21 720.2 0 21 32 11 720.2 0 11 33 16 720.2 0 16 34 13 720.2 0 13 35 19 720.2 0 19 36 17 720.2 0 17 37 28 720.2 0 28 38 110 720.2 0 110 39 34 720.2 0 34 40 61 720.2 0 61 41 79 720.2 0 79 42 74 720.2 0 74 43 127 720.2 0 127 44 70 720.2 0 70 45 40 720.2 0 40 46 29 720.2 0 29 47 43 720.2 0 43 48 99 720.2 0 99 49 112 720.2 0 112 50 207 720.2 0 207 51 448 720.2 0 448 52 388 720.2 0 388 53 678 720.2 0 678 54 1003 720.2 0 1003 55 20431 720.2 0 20431 56 6138 720.2 0 6138 57 35656 720.2 0 35656 58 112 720.2 0 112 59 21 720.2 0 21 60 20 720.2 0 20 61 22 720.2 0 22 62 17 720.2 0 17 63 36 720.2 0 36 64 18 720.2 0 18 65 22 720.2 0 22 66 19 720.2 0 19 67 21 720.2 0 21 68 13 720.2 0 13 69 29 720.2 0 29 70 14 720.2 0 14 71 12 720.2 0 12 72 22 720.2 0 22 73 21 720.2 0 21 74 29 720.2 0 29 75 35 720.2 0 35 76 42 720.2 0 42 77 28 720.2 0 28 78 82 720.2 0 82 79 14 720.2 0 14 80 23 720.2 0 23 81 35 720.2 0 35 82 41 720.2 0 41 83 40 720.2 0 40 84 63 720.2 0 63 85 769 720.2 0 769 86 396 720.2 0 396 87 1551 720.2 0 1551 88 33 720.2 0 33 89 19 720.2 0 19 90 22 720.2 0 22 91 30 720.2 0 30 92 88 720.2 0 88 93 20 720.2 0 20 94 21 720.2 0 21 95 14 720.2 0 14 96 325 720.2 0 325 97 26 720.2 0 26 98 8 720.2 0 8 99 24 720.2 0 24 100 20 720.2 0 20 101 29 720.2 0 29 102 124 720.2 0 124 103 71 720.2 0 71 104 171 720.2 0 171 105 16 720.2 0 16 106 30 720.2 0 30 107 21 720.2 0 21 108 28 720.2 0 28 109 13 720.2 0 13 110 35 720.2 0 35 111 26 720.2 0 26 112 26 720.2 0 26 113 17 720.2 0 17 114 17 720.2 0 17 115 13 720.2 0 13 116 10 720.2 0 10 117 28 720.2 0 28 118 85 720.2 0 85 119 188 720.2 0 188 120 95 720.2 0 95 121 18 720.2 0 18 122 17 720.2 0 17 123 17 720.2 0 17 124 22 720.2 0 22 125 22 720.2 0 22 126 53 720.2 0 53 127 19 720.2 0 19 128 21 720.2 0 21 129 106 720.2 0 106 130 40 720.2 0 40 131 24 720.2 0 24 132 28 720.2 0 28 133 36 720.2 0 36 134 24 720.2 0 24 135 25 720.2 0 25 136 17 720.2 0 17 137 46 720.2 0 46 138 87 720.2 0 87 139 113 720.2 0 113 140 39 720.2 0 39 141 24 720.2 0 24 142 30 720.2 0 30 143 14 720.2 0 14 144 23 720.2 0 23 145 24 720.2 0 24 146 28 720.2 0 28 147 102 720.2 0 102 148 55 720.2 0 55 149 49 720.2 0 49 150 24 720.2 0 24 151 49 720.2 0 49 152 20 720.2 0 20 153 25 720.2 0 25 154 35 720.2 0 35 155 47 720.2 0 47 156 52 720.2 0 52 157 96 720.2 0 96 158 101 720.2 0 101 159 107 720.2 0 107 160 29 720.2 0 29 161 50 720.2 0 50 162 25 720.2 0 25 163 46 720.2 0 46 164 45 720.2 0 45 165 69 720.2 0 69 166 56 720.2 0 56 167 61 720.2 0 61 168 82 720.2 0 82 169 57 720.2 0 57 170 31 720.2 0 31 171 19 720.2 0 19 172 52 720.2 0 52 173 43 720.2 0 43 174 36 720.2 0 36 175 19 720.2 0 19 176 54 720.2 0 54 177 24 720.2 0 24 178 24 720.2 0 24 179 10 720.2 0 10 180 18 720.2 0 18 181 23 720.2 0 23 182 28 720.2 0 28 183 48 720.2 0 48 184 43 720.2 0 43 185 26 720.2 0 26 186 30 720.2 0 30 187 240 720.2 0 240 188 149 720.2 0 149 189 173 720.2 0 173 190 141 720.2 0 141 191 40 720.2 0 40 192 108 720.2 0 108 193 11 720.2 0 11 194 17 720.2 0 17 195 32 720.2 0 32 196 13 720.2 0 13 197 9 720.2 0 9 198 19 720.2 0 19 199 55 720.2 0 55 200 32 720.2 0 32 201 33 720.2 0 33 202 22 720.2 0 22 203 24 720.2 0 24 204 20 720.2 0 20 205 20 720.2 0 20 206 27 720.2 0 27 207 35 720.2 0 35 208 52 720.2 0 52 209 139 720.2 0 139 210 23 720.2 0 23 211 17 720.2 0 17 212 20 720.2 0 20 213 21 720.2 0 21 214 38 720.2 0 38 215 33 720.2 0 33 216 26 720.2 0 26 217 15 720.2 0 15 218 28 720.2 0 28 219 186 720.2 0 186 220 152 720.2 0 152 221 295 720.2 0 295 222 23 720.2 0 23 223 27 720.2 0 27 224 55 720.2 0 55 225 51 720.2 0 51 226 1369 720.2 0 1369 227 15 720.2 0 15 228 18 720.2 0 18 229 25 720.2 0 25 230 18 720.2 0 18 231 24 720.2 0 24 232 19 720.2 0 19 233 39 720.2 0 39 234 19 720.2 0 19 235 42 720.2 0 42 236 23 720.2 0 23 237 17 720.2 0 17 238 23 720.2 0 23 239 48 720.2 0 48 240 80 720.2 0 80 241 68 720.2 0 68 242 27 720.2 0 27 243 23 720.2 0 23 244 15 720.2 0 15 245 14 720.2 0 14 246 37 720.2 0 37 247 29 720.2 0 29 248 30 720.2 0 30 249 32 720.2 0 32 250 60 720.2 0 60 251 47 720.2 0 47 252 39 720.2 0 39 253 18 720.2 0 18 254 19 720.2 0 19 255 12 720.2 0 12 256 14 720.2 0 14 257 21 720.2 0 21 258 46 720.2 0 46 259 76 720.2 0 76 260 139 720.2 0 139 261 138 720.2 0 138 262 75 720.2 0 75 263 27 720.2 0 27 264 23 720.2 0 23 265 32 720.2 0 32 266 23 720.2 0 23 267 28 720.2 0 28 268 35 720.2 0 35 269 28 720.2 0 28 270 28 720.2 0 28 271 24 720.2 0 24 272 45 720.2 0 45 273 32 720.2 0 32 274 60 720.2 0 60 275 60 720.2 0 60 276 539 720.2 0 539 277 215 720.2 0 215 278 1166 720.2 0 1166 279 195 720.2 0 195 280 430 720.2 0 430 281 68 720.2 0 68 282 57 720.2 0 57 283 49 720.2 0 49 284 69 720.2 0 69 285 55 720.2 0 55 286 43 720.2 0 43 287 137 720.2 0 137 288 247 720.2 0 247 289 109 720.2 0 109 290 63 720.2 0 63 291 78 720.2 0 78 292 53 720.2 0 53 293 55 720.2 0 55 294 68 720.2 0 68 295 90 720.2 0 90 296 103 720.2 0 103 297 90 720.2 0 90 298 946 720.2 0 946 299 1473 720.2 0 1473 300 1699 720.2 0 1699 301 43 720.2 0 43 Finished in 5.76 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 88,265 Reads with adapters: 8,009 (9.1%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 7,996 (9.1%) Total basepairs processed: 21,679,739 bp Total written (filtered): 2,167,404 bp (10.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8009 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 0 2 19 28 0.0 3 0 26 2 20 6422 0.0 4 6327 88 5 1 1 21 17 0.0 4 6 10 1 22 8 0.0 4 2 5 1 23 1363 0.0 4 1344 12 2 3 2 24 2 0.0 4 0 2 26 1 0.0 4 0 0 0 1 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 42 1 0.0 4 1 44 1 0.0 4 1 45 3 0.0 4 3 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 59 129 0.0 4 0 0 0 0 129 81 1 0.0 4 1 83 1 0.0 4 0 0 0 0 1 94 9 0.0 4 0 0 0 0 9 114 1 0.0 4 1 123 2 0.0 4 0 0 0 0 2 124 2 0.0 4 0 0 0 0 2 127 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 1 163 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 1 188 1 0.0 4 0 0 0 0 1 210 5 0.0 4 0 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 7,996 Reads with adapters: 923 (11.5%) Reads written (passing filters): 7,996 (100.0%) Total basepairs processed: 2,167,404 bp Total written (filtered): 2,158,001 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 923 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.5% C: 2.1% G: 96.1% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 2 31.2 0 2 9 303 0.0 0 6 297 10 272 0.0 1 11 261 11 344 0.0 1 0 344 13 1 0.0 1 0 1 151 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.