This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 129.87 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 73,990 (2.5%) Reads that were too short: 16,178 (0.5%) Reads written (passing filters): 57,812 (2.0%) Total basepairs processed: 887,907,860 bp Total written (filtered): 10,186,215 bp (1.1%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 73990 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 84 720.2 0 84 7 1193 720.2 0 1193 8 1321 720.2 0 1321 9 64 720.2 0 64 10 53 720.2 0 53 11 52 720.2 0 52 12 51 720.2 0 51 13 44 720.2 0 44 14 65 720.2 0 65 15 60 720.2 0 60 16 34 720.2 0 34 17 74 720.2 0 74 18 65 720.2 0 65 19 33 720.2 0 33 20 50 720.2 0 50 21 61 720.2 0 61 22 71 720.2 0 71 23 55 720.2 0 55 24 52 720.2 0 52 25 68 720.2 0 68 26 77 720.2 0 77 27 79 720.2 0 79 28 103 720.2 0 103 29 89 720.2 0 89 30 35 720.2 0 35 31 69 720.2 0 69 32 69 720.2 0 69 33 65 720.2 0 65 34 68 720.2 0 68 35 88 720.2 0 88 36 492 720.2 0 492 37 64 720.2 0 64 38 67 720.2 0 67 39 73 720.2 0 73 40 56 720.2 0 56 41 88 720.2 0 88 42 65 720.2 0 65 43 54 720.2 0 54 44 73 720.2 0 73 45 70 720.2 0 70 46 57 720.2 0 57 47 61 720.2 0 61 48 58 720.2 0 58 49 71 720.2 0 71 50 65 720.2 0 65 51 56 720.2 0 56 52 94 720.2 0 94 53 85 720.2 0 85 54 76 720.2 0 76 55 63 720.2 0 63 56 88 720.2 0 88 57 81 720.2 0 81 58 308 720.2 0 308 59 218 720.2 0 218 60 513 720.2 0 513 61 58 720.2 0 58 62 68 720.2 0 68 63 75 720.2 0 75 64 48 720.2 0 48 65 48 720.2 0 48 66 43 720.2 0 43 67 44 720.2 0 44 68 76 720.2 0 76 69 101 720.2 0 101 70 187 720.2 0 187 71 115 720.2 0 115 72 1142 720.2 0 1142 73 285 720.2 0 285 74 1384 720.2 0 1384 75 82 720.2 0 82 76 51 720.2 0 51 77 63 720.2 0 63 78 70 720.2 0 70 79 63 720.2 0 63 80 45 720.2 0 45 81 58 720.2 0 58 82 41 720.2 0 41 83 58 720.2 0 58 84 54 720.2 0 54 85 52 720.2 0 52 86 54 720.2 0 54 87 68 720.2 0 68 88 82 720.2 0 82 89 62 720.2 0 62 90 138 720.2 0 138 91 118 720.2 0 118 92 110 720.2 0 110 93 110 720.2 0 110 94 67 720.2 0 67 95 59 720.2 0 59 96 120 720.2 0 120 97 58 720.2 0 58 98 51 720.2 0 51 99 73 720.2 0 73 100 57 720.2 0 57 101 71 720.2 0 71 102 68 720.2 0 68 103 63 720.2 0 63 104 89 720.2 0 89 105 54 720.2 0 54 106 82 720.2 0 82 107 74 720.2 0 74 108 75 720.2 0 75 109 95 720.2 0 95 110 56 720.2 0 56 111 56 720.2 0 56 112 137 720.2 0 137 113 199 720.2 0 199 114 166 720.2 0 166 115 51 720.2 0 51 116 67 720.2 0 67 117 66 720.2 0 66 118 59 720.2 0 59 119 91 720.2 0 91 120 76 720.2 0 76 121 100 720.2 0 100 122 91 720.2 0 91 123 124 720.2 0 124 124 76 720.2 0 76 125 337 720.2 0 337 126 98 720.2 0 98 127 97 720.2 0 97 128 140 720.2 0 140 129 232 720.2 0 232 130 388 720.2 0 388 131 124 720.2 0 124 132 142 720.2 0 142 133 200 720.2 0 200 134 152 720.2 0 152 135 137 720.2 0 137 136 200 720.2 0 200 137 166 720.2 0 166 138 182 720.2 0 182 139 175 720.2 0 175 140 206 720.2 0 206 141 1674 720.2 0 1674 142 439 720.2 0 439 143 6710 720.2 0 6710 144 9911 720.2 0 9911 145 10170 720.2 0 10170 146 166 720.2 0 166 147 94 720.2 0 94 148 105 720.2 0 105 149 95 720.2 0 95 150 82 720.2 0 82 151 74 720.2 0 74 152 111 720.2 0 111 153 80 720.2 0 80 154 90 720.2 0 90 155 392 720.2 0 392 156 1691 720.2 0 1691 157 4057 720.2 0 4057 158 102 720.2 0 102 159 65 720.2 0 65 160 65 720.2 0 65 161 74 720.2 0 74 162 115 720.2 0 115 163 425 720.2 0 425 164 726 720.2 0 726 165 163 720.2 0 163 166 77 720.2 0 77 167 64 720.2 0 64 168 67 720.2 0 67 169 48 720.2 0 48 170 70 720.2 0 70 171 68 720.2 0 68 172 42 720.2 0 42 173 61 720.2 0 61 174 61 720.2 0 61 175 55 720.2 0 55 176 65 720.2 0 65 177 67 720.2 0 67 178 72 720.2 0 72 179 57 720.2 0 57 180 58 720.2 0 58 181 49 720.2 0 49 182 62 720.2 0 62 183 60 720.2 0 60 184 45 720.2 0 45 185 64 720.2 0 64 186 59 720.2 0 59 187 55 720.2 0 55 188 95 720.2 0 95 189 71 720.2 0 71 190 58 720.2 0 58 191 51 720.2 0 51 192 53 720.2 0 53 193 76 720.2 0 76 194 61 720.2 0 61 195 76 720.2 0 76 196 73 720.2 0 73 197 60 720.2 0 60 198 59 720.2 0 59 199 54 720.2 0 54 200 73 720.2 0 73 201 62 720.2 0 62 202 63 720.2 0 63 203 74 720.2 0 74 204 82 720.2 0 82 205 53 720.2 0 53 206 76 720.2 0 76 207 79 720.2 0 79 208 72 720.2 0 72 209 106 720.2 0 106 210 128 720.2 0 128 211 175 720.2 0 175 212 3133 720.2 0 3133 213 156 720.2 0 156 214 150 720.2 0 150 215 1111 720.2 0 1111 216 642 720.2 0 642 217 3825 720.2 0 3825 218 72 720.2 0 72 219 55 720.2 0 55 220 66 720.2 0 66 221 75 720.2 0 75 222 85 720.2 0 85 223 65 720.2 0 65 224 84 720.2 0 84 225 78 720.2 0 78 226 49 720.2 0 49 227 81 720.2 0 81 228 73 720.2 0 73 229 51 720.2 0 51 230 62 720.2 0 62 231 84 720.2 0 84 232 86 720.2 0 86 233 95 720.2 0 95 234 63 720.2 0 63 235 59 720.2 0 59 236 93 720.2 0 93 237 74 720.2 0 74 238 45 720.2 0 45 239 70 720.2 0 70 240 67 720.2 0 67 241 73 720.2 0 73 242 55 720.2 0 55 243 62 720.2 0 62 244 72 720.2 0 72 245 87 720.2 0 87 246 75 720.2 0 75 247 67 720.2 0 67 248 46 720.2 0 46 249 62 720.2 0 62 250 49 720.2 0 49 251 60 720.2 0 60 252 48 720.2 0 48 253 71 720.2 0 71 254 76 720.2 0 76 255 97 720.2 0 97 256 65 720.2 0 65 257 72 720.2 0 72 258 69 720.2 0 69 259 73 720.2 0 73 260 91 720.2 0 91 261 77 720.2 0 77 262 43 720.2 0 43 263 82 720.2 0 82 264 81 720.2 0 81 265 66 720.2 0 66 266 52 720.2 0 52 267 58 720.2 0 58 268 59 720.2 0 59 269 68 720.2 0 68 270 54 720.2 0 54 271 73 720.2 0 73 272 67 720.2 0 67 273 61 720.2 0 61 274 68 720.2 0 68 275 60 720.2 0 60 276 61 720.2 0 61 277 78 720.2 0 78 278 86 720.2 0 86 279 73 720.2 0 73 280 83 720.2 0 83 281 85 720.2 0 85 282 100 720.2 0 100 283 91 720.2 0 91 284 88 720.2 0 88 285 97 720.2 0 97 286 109 720.2 0 109 287 76 720.2 0 76 288 63 720.2 0 63 289 47 720.2 0 47 290 72 720.2 0 72 291 60 720.2 0 60 292 68 720.2 0 68 293 61 720.2 0 61 294 82 720.2 0 82 295 134 720.2 0 134 296 145 720.2 0 145 297 253 720.2 0 253 298 80 720.2 0 80 299 64 720.2 0 64 300 73 720.2 0 73 301 53 720.2 0 53 Finished in 3.28 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 57,812 Reads with adapters: 1,373 (2.4%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 1,364 (2.4%) Total basepairs processed: 10,186,215 bp Total written (filtered): 371,956 bp (3.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1373 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 14 0.0 3 0 13 1 20 1338 0.0 4 1307 30 1 21 6 0.0 4 2 4 25 1 0.0 4 1 63 1 0.0 4 1 75 1 0.0 4 1 77 1 0.0 4 1 115 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 147 2 0.0 4 0 0 0 0 2 152 1 0.0 4 1 213 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 1,364 Reads with adapters: 167 (12.2%) Reads written (passing filters): 1,364 (100.0%) Total basepairs processed: 371,956 bp Total written (filtered): 352,514 bp (94.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 167 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 28.7% C: 7.8% G: 61.7% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 21.3 0 1 4 1 5.3 0 1 10 1 0.0 1 0 1 11 96 0.0 1 0 96 270 59 0.0 1 56 3 271 9 0.0 1 8 1