This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.60 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 235,507 (8.9%) Reads that were too short: 23,856 (0.9%) Reads written (passing filters): 211,651 (8.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 39,905,616 bp (5.0%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 235507 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 649.0 0 208 7 7922 649.0 0 7922 8 7166 649.0 0 7166 9 51 649.0 0 51 10 30 649.0 0 30 11 34 649.0 0 34 12 171 649.0 0 171 13 145 649.0 0 145 14 129 649.0 0 129 15 41 649.0 0 41 16 40 649.0 0 40 17 33 649.0 0 33 18 35 649.0 0 35 19 36 649.0 0 36 20 34 649.0 0 34 21 35 649.0 0 35 22 50 649.0 0 50 23 47 649.0 0 47 24 37 649.0 0 37 25 405 649.0 0 405 26 175 649.0 0 175 27 97 649.0 0 97 28 62 649.0 0 62 29 88 649.0 0 88 30 62 649.0 0 62 31 37 649.0 0 37 32 70 649.0 0 70 33 51 649.0 0 51 34 38 649.0 0 38 35 114 649.0 0 114 36 74 649.0 0 74 37 60 649.0 0 60 38 41 649.0 0 41 39 48 649.0 0 48 40 58 649.0 0 58 41 47 649.0 0 47 42 71 649.0 0 71 43 83 649.0 0 83 44 70 649.0 0 70 45 35 649.0 0 35 46 57 649.0 0 57 47 83 649.0 0 83 48 58 649.0 0 58 49 74 649.0 0 74 50 177 649.0 0 177 51 66 649.0 0 66 52 50 649.0 0 50 53 64 649.0 0 64 54 93 649.0 0 93 55 80 649.0 0 80 56 120 649.0 0 120 57 67 649.0 0 67 58 81 649.0 0 81 59 58 649.0 0 58 60 62 649.0 0 62 61 81 649.0 0 81 62 99 649.0 0 99 63 74 649.0 0 74 64 89 649.0 0 89 65 479 649.0 0 479 66 289 649.0 0 289 67 324 649.0 0 324 68 78 649.0 0 78 69 60 649.0 0 60 70 63 649.0 0 63 71 55 649.0 0 55 72 88 649.0 0 88 73 75 649.0 0 75 74 62 649.0 0 62 75 55 649.0 0 55 76 50 649.0 0 50 77 85 649.0 0 85 78 78 649.0 0 78 79 183 649.0 0 183 80 144 649.0 0 144 81 148 649.0 0 148 82 122 649.0 0 122 83 105 649.0 0 105 84 75 649.0 0 75 85 72 649.0 0 72 86 151 649.0 0 151 87 158 649.0 0 158 88 212 649.0 0 212 89 283 649.0 0 283 90 414 649.0 0 414 91 779 649.0 0 779 92 318 649.0 0 318 93 402 649.0 0 402 94 522 649.0 0 522 95 292 649.0 0 292 96 296 649.0 0 296 97 404 649.0 0 404 98 442 649.0 0 442 99 599 649.0 0 599 100 554 649.0 0 554 101 911 649.0 0 911 102 1370 649.0 0 1370 103 3114 649.0 0 3114 104 51355 649.0 0 51355 105 30461 649.0 0 30461 106 30481 649.0 0 30481 107 181 649.0 0 181 108 51 649.0 0 51 109 55 649.0 0 55 110 72 649.0 0 72 111 84 649.0 0 84 112 101 649.0 0 101 113 114 649.0 0 114 114 155 649.0 0 155 115 763 649.0 0 763 116 406 649.0 0 406 117 139 649.0 0 139 118 101 649.0 0 101 119 209 649.0 0 209 120 116 649.0 0 116 121 160 649.0 0 160 122 69 649.0 0 69 123 100 649.0 0 100 124 211 649.0 0 211 125 189 649.0 0 189 126 302 649.0 0 302 127 168 649.0 0 168 128 147 649.0 0 147 129 92 649.0 0 92 130 115 649.0 0 115 131 106 649.0 0 106 132 133 649.0 0 133 133 123 649.0 0 123 134 118 649.0 0 118 135 72 649.0 0 72 136 98 649.0 0 98 137 67 649.0 0 67 138 187 649.0 0 187 139 191 649.0 0 191 140 192 649.0 0 192 141 133 649.0 0 133 142 173 649.0 0 173 143 138 649.0 0 138 144 290 649.0 0 290 145 167 649.0 0 167 146 418 649.0 0 418 147 207 649.0 0 207 148 142 649.0 0 142 149 160 649.0 0 160 150 98 649.0 0 98 151 213 649.0 0 213 152 283 649.0 0 283 153 364 649.0 0 364 154 299 649.0 0 299 155 454 649.0 0 454 156 479 649.0 0 479 157 915 649.0 0 915 158 1541 649.0 0 1541 159 19532 649.0 0 19532 160 11952 649.0 0 11952 161 15398 649.0 0 15398 162 146 649.0 0 146 163 106 649.0 0 106 164 82 649.0 0 82 165 103 649.0 0 103 166 113 649.0 0 113 167 239 649.0 0 239 168 154 649.0 0 154 169 191 649.0 0 191 170 76 649.0 0 76 171 116 649.0 0 116 172 214 649.0 0 214 173 231 649.0 0 231 174 184 649.0 0 184 175 101 649.0 0 101 176 82 649.0 0 82 177 138 649.0 0 138 178 106 649.0 0 106 179 105 649.0 0 105 180 228 649.0 0 228 181 159 649.0 0 159 182 129 649.0 0 129 183 134 649.0 0 134 184 325 649.0 0 325 185 207 649.0 0 207 186 288 649.0 0 288 187 115 649.0 0 115 188 175 649.0 0 175 189 200 649.0 0 200 190 161 649.0 0 161 191 95 649.0 0 95 192 107 649.0 0 107 193 105 649.0 0 105 194 119 649.0 0 119 195 127 649.0 0 127 196 221 649.0 0 221 197 195 649.0 0 195 198 190 649.0 0 190 199 251 649.0 0 251 200 618 649.0 0 618 201 501 649.0 0 501 202 442 649.0 0 442 203 115 649.0 0 115 204 155 649.0 0 155 205 292 649.0 0 292 206 219 649.0 0 219 207 283 649.0 0 283 208 187 649.0 0 187 209 222 649.0 0 222 210 91 649.0 0 91 211 94 649.0 0 94 212 96 649.0 0 96 213 126 649.0 0 126 214 134 649.0 0 134 215 128 649.0 0 128 216 139 649.0 0 139 217 115 649.0 0 115 218 203 649.0 0 203 219 259 649.0 0 259 220 231 649.0 0 231 221 187 649.0 0 187 222 156 649.0 0 156 223 231 649.0 0 231 224 118 649.0 0 118 225 109 649.0 0 109 226 169 649.0 0 169 227 162 649.0 0 162 228 241 649.0 0 241 229 211 649.0 0 211 230 187 649.0 0 187 231 171 649.0 0 171 232 126 649.0 0 126 233 109 649.0 0 109 234 111 649.0 0 111 235 103 649.0 0 103 236 146 649.0 0 146 237 232 649.0 0 232 238 401 649.0 0 401 239 389 649.0 0 389 240 1032 649.0 0 1032 241 1163 649.0 0 1163 242 991 649.0 0 991 243 440 649.0 0 440 244 114 649.0 0 114 245 114 649.0 0 114 246 147 649.0 0 147 247 126 649.0 0 126 248 148 649.0 0 148 249 138 649.0 0 138 250 127 649.0 0 127 251 157 649.0 0 157 252 166 649.0 0 166 253 147 649.0 0 147 254 241 649.0 0 241 255 316 649.0 0 316 256 303 649.0 0 303 257 246 649.0 0 246 258 161 649.0 0 161 259 133 649.0 0 133 260 86 649.0 0 86 261 106 649.0 0 106 262 110 649.0 0 110 263 136 649.0 0 136 264 100 649.0 0 100 265 137 649.0 0 137 266 157 649.0 0 157 267 205 649.0 0 205 268 229 649.0 0 229 269 233 649.0 0 233 270 208 649.0 0 208 271 131 649.0 0 131 272 151 649.0 0 151 273 188 649.0 0 188 274 248 649.0 0 248 275 245 649.0 0 245 276 233 649.0 0 233 277 203 649.0 0 203 278 679 649.0 0 679 279 447 649.0 0 447 280 578 649.0 0 578 281 685 649.0 0 685 282 549 649.0 0 549 283 736 649.0 0 736 284 198 649.0 0 198 285 148 649.0 0 148 286 105 649.0 0 105 287 151 649.0 0 151 288 183 649.0 0 183 289 168 649.0 0 168 290 157 649.0 0 157 291 171 649.0 0 171 292 217 649.0 0 217 293 200 649.0 0 200 294 252 649.0 0 252 295 232 649.0 0 232 296 193 649.0 0 193 297 131 649.0 0 131 298 127 649.0 0 127 299 262 649.0 0 262 300 398 649.0 0 398 301 283 649.0 0 283 Finished in 7.42 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 211,651 Reads with adapters: 8,912 (4.2%) Reads that were too short: 347 (0.2%) Reads written (passing filters): 8,565 (4.0%) Total basepairs processed: 39,905,616 bp Total written (filtered): 2,248,681 bp (5.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8912 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 3 3 16 86 0.0 3 0 73 11 2 17 7647 0.0 3 7165 396 69 17 18 20 0.0 3 2 13 1 4 19 40 0.0 3 2 0 30 8 20 22 0.0 3 4 1 0 17 22 1 0.0 3 0 0 0 1 23 35 0.0 3 0 0 1 34 26 3 0.0 3 0 0 1 2 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 31 1 0.0 3 0 1 32 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 42 4 0.0 3 0 0 0 4 43 1 0.0 3 0 0 0 1 44 5 0.0 3 0 0 0 5 45 1 0.0 3 0 0 0 1 46 258 0.0 3 0 0 4 254 47 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 52 5 0.0 3 0 0 0 5 53 4 0.0 3 0 0 0 4 54 7 0.0 3 0 0 0 7 56 1 0.0 3 0 0 0 1 58 35 0.0 3 0 0 1 34 61 1 0.0 3 0 0 0 1 62 5 0.0 3 0 0 0 5 64 2 0.0 3 0 0 2 65 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 70 5 0.0 3 0 0 0 5 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 75 4 0.0 3 0 0 0 4 77 2 0.0 3 0 0 0 2 78 3 0.0 3 0 0 0 3 80 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 86 4 0.0 3 0 0 0 4 87 108 0.0 3 0 0 0 108 88 119 0.0 3 0 0 2 117 89 3 0.0 3 0 0 0 3 90 31 0.0 3 0 0 1 30 91 37 0.0 3 0 0 2 35 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 3 0.0 3 0 0 0 3 104 3 0.0 3 0 0 1 2 109 1 0.0 3 0 0 0 1 110 5 0.0 3 0 0 0 5 111 6 0.0 3 0 0 2 4 112 8 0.0 3 0 0 0 8 113 8 0.0 3 0 0 0 8 114 4 0.0 3 0 0 0 4 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 7 0.0 3 0 0 0 7 125 5 0.0 3 0 0 1 4 126 12 0.0 3 0 0 1 11 127 1 0.0 3 0 0 0 1 128 11 0.0 3 0 0 0 11 129 7 0.0 3 0 0 0 7 130 17 0.0 3 0 0 0 17 131 12 0.0 3 0 0 1 11 132 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 1 2 137 4 0.0 3 0 0 0 4 138 21 0.0 3 0 0 0 21 139 13 0.0 3 0 0 0 13 140 2 0.0 3 0 0 0 2 141 2 0.0 3 0 0 0 2 142 20 0.0 3 0 0 0 20 146 3 0.0 3 0 0 0 3 147 8 0.0 3 0 0 0 8 148 8 0.0 3 0 0 0 8 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 159 5 0.0 3 0 0 0 5 160 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 5 0.0 3 0 0 0 5 167 5 0.0 3 0 0 0 5 170 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 0 3 180 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 4 0.0 3 0 0 0 4 188 34 0.0 3 0 0 3 31 189 55 0.0 3 0 0 5 50 190 7 0.0 3 0 0 0 7 191 2 0.0 3 0 0 0 2 196 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 1 244 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 8,565 Reads with adapters: 34 (0.4%) Reads written (passing filters): 8,565 (100.0%) Total basepairs processed: 2,248,681 bp Total written (filtered): 2,248,573 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 34 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.5% C: 32.4% G: 23.5% T: 20.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 28 133.8 0 28 4 6 33.5 0 6