This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.01 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 244,803 (9.2%) Reads that were too short: 24,890 (0.9%) Reads written (passing filters): 219,913 (8.3%) Total basepairs processed: 800,111,879 bp Total written (filtered): 42,386,547 bp (5.3%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 244803 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 504 649.0 0 504 7 15176 649.0 0 15176 8 12844 649.0 0 12844 9 66 649.0 0 66 10 33 649.0 0 33 11 54 649.0 0 54 12 62 649.0 0 62 13 40 649.0 0 40 14 35 649.0 0 35 15 43 649.0 0 43 16 44 649.0 0 44 17 42 649.0 0 42 18 33 649.0 0 33 19 29 649.0 0 29 20 50 649.0 0 50 21 34 649.0 0 34 22 39 649.0 0 39 23 38 649.0 0 38 24 37 649.0 0 37 25 41 649.0 0 41 26 41 649.0 0 41 27 62 649.0 0 62 28 57 649.0 0 57 29 138 649.0 0 138 30 80 649.0 0 80 31 76 649.0 0 76 32 37 649.0 0 37 33 49 649.0 0 49 34 43 649.0 0 43 35 45 649.0 0 45 36 56 649.0 0 56 37 60 649.0 0 60 38 117 649.0 0 117 39 61 649.0 0 61 40 53 649.0 0 53 41 37 649.0 0 37 42 39 649.0 0 39 43 45 649.0 0 45 44 57 649.0 0 57 45 68 649.0 0 68 46 47 649.0 0 47 47 38 649.0 0 38 48 54 649.0 0 54 49 69 649.0 0 69 50 52 649.0 0 52 51 47 649.0 0 47 52 46 649.0 0 46 53 52 649.0 0 52 54 46 649.0 0 46 55 57 649.0 0 57 56 71 649.0 0 71 57 68 649.0 0 68 58 60 649.0 0 60 59 89 649.0 0 89 60 66 649.0 0 66 61 102 649.0 0 102 62 68 649.0 0 68 63 85 649.0 0 85 64 180 649.0 0 180 65 81 649.0 0 81 66 90 649.0 0 90 67 90 649.0 0 90 68 133 649.0 0 133 69 165 649.0 0 165 70 2126 649.0 0 2126 71 362 649.0 0 362 72 1015 649.0 0 1015 73 101 649.0 0 101 74 81 649.0 0 81 75 74 649.0 0 74 76 166 649.0 0 166 77 103 649.0 0 103 78 183 649.0 0 183 79 188 649.0 0 188 80 138 649.0 0 138 81 227 649.0 0 227 82 525 649.0 0 525 83 1551 649.0 0 1551 84 191 649.0 0 191 85 138 649.0 0 138 86 92 649.0 0 92 87 73 649.0 0 73 88 79 649.0 0 79 89 57 649.0 0 57 90 57 649.0 0 57 91 67 649.0 0 67 92 95 649.0 0 95 93 88 649.0 0 88 94 77 649.0 0 77 95 80 649.0 0 80 96 100 649.0 0 100 97 146 649.0 0 146 98 190 649.0 0 190 99 277 649.0 0 277 100 241 649.0 0 241 101 124 649.0 0 124 102 121 649.0 0 121 103 147 649.0 0 147 104 120 649.0 0 120 105 106 649.0 0 106 106 108 649.0 0 108 107 215 649.0 0 215 108 233 649.0 0 233 109 256 649.0 0 256 110 449 649.0 0 449 111 1001 649.0 0 1001 112 349 649.0 0 349 113 431 649.0 0 431 114 673 649.0 0 673 115 392 649.0 0 392 116 381 649.0 0 381 117 639 649.0 0 639 118 663 649.0 0 663 119 832 649.0 0 832 120 863 649.0 0 863 121 1374 649.0 0 1374 122 1704 649.0 0 1704 123 3105 649.0 0 3105 124 65305 649.0 0 65305 125 23461 649.0 0 23461 126 59129 649.0 0 59129 127 1551 649.0 0 1551 128 160 649.0 0 160 129 186 649.0 0 186 130 146 649.0 0 146 131 136 649.0 0 136 132 98 649.0 0 98 133 111 649.0 0 111 134 125 649.0 0 125 135 150 649.0 0 150 136 199 649.0 0 199 137 145 649.0 0 145 138 149 649.0 0 149 139 228 649.0 0 228 140 426 649.0 0 426 141 275 649.0 0 275 142 739 649.0 0 739 143 125 649.0 0 125 144 164 649.0 0 164 145 180 649.0 0 180 146 150 649.0 0 150 147 157 649.0 0 157 148 198 649.0 0 198 149 1777 649.0 0 1777 150 941 649.0 0 941 151 1578 649.0 0 1578 152 129 649.0 0 129 153 98 649.0 0 98 154 121 649.0 0 121 155 121 649.0 0 121 156 149 649.0 0 149 157 156 649.0 0 156 158 155 649.0 0 155 159 132 649.0 0 132 160 129 649.0 0 129 161 102 649.0 0 102 162 163 649.0 0 163 163 168 649.0 0 168 164 237 649.0 0 237 165 161 649.0 0 161 166 129 649.0 0 129 167 94 649.0 0 94 168 90 649.0 0 90 169 133 649.0 0 133 170 87 649.0 0 87 171 105 649.0 0 105 172 94 649.0 0 94 173 75 649.0 0 75 174 89 649.0 0 89 175 100 649.0 0 100 176 94 649.0 0 94 177 115 649.0 0 115 178 111 649.0 0 111 179 114 649.0 0 114 180 107 649.0 0 107 181 277 649.0 0 277 182 217 649.0 0 217 183 201 649.0 0 201 184 103 649.0 0 103 185 118 649.0 0 118 186 126 649.0 0 126 187 132 649.0 0 132 188 224 649.0 0 224 189 115 649.0 0 115 190 174 649.0 0 174 191 89 649.0 0 89 192 84 649.0 0 84 193 94 649.0 0 94 194 157 649.0 0 157 195 96 649.0 0 96 196 113 649.0 0 113 197 111 649.0 0 111 198 154 649.0 0 154 199 93 649.0 0 93 200 94 649.0 0 94 201 99 649.0 0 99 202 96 649.0 0 96 203 190 649.0 0 190 204 150 649.0 0 150 205 200 649.0 0 200 206 150 649.0 0 150 207 159 649.0 0 159 208 152 649.0 0 152 209 139 649.0 0 139 210 111 649.0 0 111 211 126 649.0 0 126 212 136 649.0 0 136 213 118 649.0 0 118 214 147 649.0 0 147 215 113 649.0 0 113 216 127 649.0 0 127 217 210 649.0 0 210 218 172 649.0 0 172 219 169 649.0 0 169 220 169 649.0 0 169 221 183 649.0 0 183 222 237 649.0 0 237 223 981 649.0 0 981 224 1109 649.0 0 1109 225 3496 649.0 0 3496 226 3751 649.0 0 3751 227 3229 649.0 0 3229 228 132 649.0 0 132 229 109 649.0 0 109 230 106 649.0 0 106 231 92 649.0 0 92 232 113 649.0 0 113 233 112 649.0 0 112 234 142 649.0 0 142 235 151 649.0 0 151 236 141 649.0 0 141 237 114 649.0 0 114 238 133 649.0 0 133 239 128 649.0 0 128 240 147 649.0 0 147 241 115 649.0 0 115 242 132 649.0 0 132 243 196 649.0 0 196 244 194 649.0 0 194 245 191 649.0 0 191 246 134 649.0 0 134 247 78 649.0 0 78 248 82 649.0 0 82 249 99 649.0 0 99 250 84 649.0 0 84 251 106 649.0 0 106 252 114 649.0 0 114 253 147 649.0 0 147 254 119 649.0 0 119 255 171 649.0 0 171 256 144 649.0 0 144 257 130 649.0 0 130 258 168 649.0 0 168 259 123 649.0 0 123 260 130 649.0 0 130 261 135 649.0 0 135 262 128 649.0 0 128 263 205 649.0 0 205 264 157 649.0 0 157 265 167 649.0 0 167 266 149 649.0 0 149 267 153 649.0 0 153 268 179 649.0 0 179 269 129 649.0 0 129 270 79 649.0 0 79 271 89 649.0 0 89 272 107 649.0 0 107 273 110 649.0 0 110 274 142 649.0 0 142 275 103 649.0 0 103 276 121 649.0 0 121 277 112 649.0 0 112 278 122 649.0 0 122 279 103 649.0 0 103 280 141 649.0 0 141 281 146 649.0 0 146 282 91 649.0 0 91 283 95 649.0 0 95 284 98 649.0 0 98 285 100 649.0 0 100 286 98 649.0 0 98 287 83 649.0 0 83 288 104 649.0 0 104 289 107 649.0 0 107 290 93 649.0 0 93 291 84 649.0 0 84 292 101 649.0 0 101 293 121 649.0 0 121 294 105 649.0 0 105 295 127 649.0 0 127 296 114 649.0 0 114 297 84 649.0 0 84 298 92 649.0 0 92 299 112 649.0 0 112 300 89 649.0 0 89 301 118 649.0 0 118 Finished in 7.91 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 219,913 Reads with adapters: 17,745 (8.1%) Reads that were too short: 404 (0.2%) Reads written (passing filters): 17,341 (7.9%) Total basepairs processed: 42,386,547 bp Total written (filtered): 4,441,857 bp (10.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17745 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 11 0.0 3 0 0 9 2 16 100 0.0 3 0 80 11 9 17 3733 0.0 3 3339 319 59 16 18 11140 0.0 3 10491 529 88 32 19 54 0.0 3 3 31 13 7 20 49 0.0 3 3 0 14 32 21 33 0.0 3 0 0 1 32 22 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 0 1 25 3 0.0 3 0 0 0 3 26 6 0.0 3 0 0 0 6 28 1 0.0 3 1 29 11 0.0 3 0 0 0 11 32 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 42 906 0.0 3 0 0 2 904 43 1 0.0 3 0 0 0 1 45 167 0.0 3 0 0 4 163 47 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 52 13 0.0 3 0 0 0 13 54 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 56 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 9 0.0 3 0 0 0 9 66 36 0.0 3 0 0 1 35 67 2 0.0 3 0 0 0 2 68 1006 0.0 3 0 0 6 1000 69 19 0.0 3 0 0 0 19 70 3 0.0 3 0 0 0 3 71 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 77 2 0.0 3 1 0 0 1 78 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 0 4 85 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 66 0.0 3 0 0 1 65 94 4 0.0 3 0 0 0 4 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 102 102 0.0 3 0 0 1 101 103 5 0.0 3 0 0 0 5 105 7 0.0 3 0 0 1 6 110 4 0.0 3 0 0 0 4 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 1 2 115 5 0.0 3 0 0 0 5 121 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 11 0.0 3 0 0 0 11 143 20 0.0 3 0 0 0 20 144 18 0.0 3 0 0 1 17 145 10 0.0 3 0 0 0 10 154 3 0.0 3 0 0 0 3 155 16 0.0 3 0 0 0 16 156 17 0.0 3 0 0 1 16 157 21 0.0 3 0 0 1 20 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 5 0.0 3 0 0 0 5 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 1 1 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 12 0.0 3 0 0 1 11 219 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 281 6 0.0 3 0 0 0 6 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 17,341 Reads with adapters: 310 (1.8%) Reads written (passing filters): 17,341 (100.0%) Total basepairs processed: 4,441,857 bp Total written (filtered): 4,440,872 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 310 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.9% C: 11.6% G: 41.6% T: 12.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 275 271.0 0 275 4 27 67.7 0 27 5 6 16.9 0 6 11 2 0.0 1 0 2