This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.98 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 236,665 (8.9%) Reads that were too short: 25,135 (0.9%) Reads written (passing filters): 211,530 (8.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 40,998,775 bp (5.1%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 236665 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 344 649.0 0 344 7 14810 649.0 0 14810 8 14541 649.0 0 14541 9 28 649.0 0 28 10 22 649.0 0 22 11 38 649.0 0 38 12 32 649.0 0 32 13 29 649.0 0 29 14 25 649.0 0 25 15 32 649.0 0 32 16 32 649.0 0 32 17 29 649.0 0 29 18 33 649.0 0 33 19 18 649.0 0 18 20 32 649.0 0 32 21 25 649.0 0 25 22 27 649.0 0 27 23 29 649.0 0 29 24 20 649.0 0 20 25 29 649.0 0 29 26 32 649.0 0 32 27 39 649.0 0 39 28 40 649.0 0 40 29 126 649.0 0 126 30 29 649.0 0 29 31 40 649.0 0 40 32 26 649.0 0 26 33 26 649.0 0 26 34 37 649.0 0 37 35 37 649.0 0 37 36 44 649.0 0 44 37 60 649.0 0 60 38 114 649.0 0 114 39 44 649.0 0 44 40 36 649.0 0 36 41 32 649.0 0 32 42 32 649.0 0 32 43 60 649.0 0 60 44 28 649.0 0 28 45 33 649.0 0 33 46 44 649.0 0 44 47 35 649.0 0 35 48 44 649.0 0 44 49 37 649.0 0 37 50 41 649.0 0 41 51 29 649.0 0 29 52 33 649.0 0 33 53 39 649.0 0 39 54 36 649.0 0 36 55 43 649.0 0 43 56 57 649.0 0 57 57 50 649.0 0 50 58 60 649.0 0 60 59 76 649.0 0 76 60 55 649.0 0 55 61 52 649.0 0 52 62 53 649.0 0 53 63 57 649.0 0 57 64 183 649.0 0 183 65 47 649.0 0 47 66 84 649.0 0 84 67 78 649.0 0 78 68 102 649.0 0 102 69 153 649.0 0 153 70 2211 649.0 0 2211 71 386 649.0 0 386 72 1052 649.0 0 1052 73 64 649.0 0 64 74 46 649.0 0 46 75 49 649.0 0 49 76 48 649.0 0 48 77 52 649.0 0 52 78 53 649.0 0 53 79 60 649.0 0 60 80 79 649.0 0 79 81 164 649.0 0 164 82 378 649.0 0 378 83 1605 649.0 0 1605 84 151 649.0 0 151 85 103 649.0 0 103 86 76 649.0 0 76 87 104 649.0 0 104 88 69 649.0 0 69 89 71 649.0 0 71 90 100 649.0 0 100 91 88 649.0 0 88 92 103 649.0 0 103 93 74 649.0 0 74 94 140 649.0 0 140 95 104 649.0 0 104 96 84 649.0 0 84 97 172 649.0 0 172 98 205 649.0 0 205 99 137 649.0 0 137 100 229 649.0 0 229 101 168 649.0 0 168 102 106 649.0 0 106 103 109 649.0 0 109 104 110 649.0 0 110 105 95 649.0 0 95 106 130 649.0 0 130 107 277 649.0 0 277 108 258 649.0 0 258 109 276 649.0 0 276 110 441 649.0 0 441 111 915 649.0 0 915 112 360 649.0 0 360 113 354 649.0 0 354 114 734 649.0 0 734 115 349 649.0 0 349 116 341 649.0 0 341 117 630 649.0 0 630 118 613 649.0 0 613 119 714 649.0 0 714 120 777 649.0 0 777 121 1184 649.0 0 1184 122 1299 649.0 0 1299 123 2269 649.0 0 2269 124 61408 649.0 0 61408 125 22001 649.0 0 22001 126 60185 649.0 0 60185 127 1443 649.0 0 1443 128 129 649.0 0 129 129 119 649.0 0 119 130 115 649.0 0 115 131 110 649.0 0 110 132 82 649.0 0 82 133 92 649.0 0 92 134 95 649.0 0 95 135 142 649.0 0 142 136 135 649.0 0 135 137 132 649.0 0 132 138 147 649.0 0 147 139 185 649.0 0 185 140 420 649.0 0 420 141 307 649.0 0 307 142 695 649.0 0 695 143 121 649.0 0 121 144 131 649.0 0 131 145 122 649.0 0 122 146 117 649.0 0 117 147 112 649.0 0 112 148 148 649.0 0 148 149 1696 649.0 0 1696 150 866 649.0 0 866 151 1641 649.0 0 1641 152 98 649.0 0 98 153 79 649.0 0 79 154 92 649.0 0 92 155 103 649.0 0 103 156 131 649.0 0 131 157 114 649.0 0 114 158 139 649.0 0 139 159 123 649.0 0 123 160 69 649.0 0 69 161 104 649.0 0 104 162 97 649.0 0 97 163 178 649.0 0 178 164 173 649.0 0 173 165 128 649.0 0 128 166 104 649.0 0 104 167 92 649.0 0 92 168 85 649.0 0 85 169 89 649.0 0 89 170 111 649.0 0 111 171 111 649.0 0 111 172 78 649.0 0 78 173 72 649.0 0 72 174 73 649.0 0 73 175 63 649.0 0 63 176 76 649.0 0 76 177 88 649.0 0 88 178 111 649.0 0 111 179 93 649.0 0 93 180 95 649.0 0 95 181 190 649.0 0 190 182 161 649.0 0 161 183 136 649.0 0 136 184 98 649.0 0 98 185 81 649.0 0 81 186 106 649.0 0 106 187 94 649.0 0 94 188 193 649.0 0 193 189 101 649.0 0 101 190 179 649.0 0 179 191 88 649.0 0 88 192 80 649.0 0 80 193 73 649.0 0 73 194 107 649.0 0 107 195 89 649.0 0 89 196 87 649.0 0 87 197 93 649.0 0 93 198 111 649.0 0 111 199 81 649.0 0 81 200 93 649.0 0 93 201 80 649.0 0 80 202 87 649.0 0 87 203 101 649.0 0 101 204 130 649.0 0 130 205 131 649.0 0 131 206 118 649.0 0 118 207 137 649.0 0 137 208 151 649.0 0 151 209 110 649.0 0 110 210 143 649.0 0 143 211 111 649.0 0 111 212 125 649.0 0 125 213 106 649.0 0 106 214 120 649.0 0 120 215 88 649.0 0 88 216 109 649.0 0 109 217 168 649.0 0 168 218 159 649.0 0 159 219 162 649.0 0 162 220 127 649.0 0 127 221 199 649.0 0 199 222 197 649.0 0 197 223 970 649.0 0 970 224 1206 649.0 0 1206 225 3942 649.0 0 3942 226 4670 649.0 0 4670 227 3873 649.0 0 3873 228 104 649.0 0 104 229 83 649.0 0 83 230 100 649.0 0 100 231 77 649.0 0 77 232 103 649.0 0 103 233 86 649.0 0 86 234 113 649.0 0 113 235 140 649.0 0 140 236 163 649.0 0 163 237 126 649.0 0 126 238 95 649.0 0 95 239 90 649.0 0 90 240 110 649.0 0 110 241 90 649.0 0 90 242 109 649.0 0 109 243 127 649.0 0 127 244 148 649.0 0 148 245 137 649.0 0 137 246 99 649.0 0 99 247 81 649.0 0 81 248 82 649.0 0 82 249 89 649.0 0 89 250 78 649.0 0 78 251 89 649.0 0 89 252 106 649.0 0 106 253 126 649.0 0 126 254 121 649.0 0 121 255 111 649.0 0 111 256 151 649.0 0 151 257 123 649.0 0 123 258 113 649.0 0 113 259 102 649.0 0 102 260 122 649.0 0 122 261 104 649.0 0 104 262 114 649.0 0 114 263 113 649.0 0 113 264 121 649.0 0 121 265 108 649.0 0 108 266 108 649.0 0 108 267 110 649.0 0 110 268 129 649.0 0 129 269 70 649.0 0 70 270 81 649.0 0 81 271 75 649.0 0 75 272 92 649.0 0 92 273 103 649.0 0 103 274 98 649.0 0 98 275 100 649.0 0 100 276 83 649.0 0 83 277 98 649.0 0 98 278 116 649.0 0 116 279 99 649.0 0 99 280 95 649.0 0 95 281 100 649.0 0 100 282 103 649.0 0 103 283 93 649.0 0 93 284 90 649.0 0 90 285 110 649.0 0 110 286 70 649.0 0 70 287 86 649.0 0 86 288 104 649.0 0 104 289 96 649.0 0 96 290 71 649.0 0 71 291 82 649.0 0 82 292 89 649.0 0 89 293 135 649.0 0 135 294 93 649.0 0 93 295 123 649.0 0 123 296 98 649.0 0 98 297 95 649.0 0 95 298 132 649.0 0 132 299 103 649.0 0 103 300 103 649.0 0 103 301 108 649.0 0 108 Finished in 8.73 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 211,530 Reads with adapters: 15,449 (7.3%) Reads that were too short: 41 (0.0%) Reads written (passing filters): 15,408 (7.3%) Total basepairs processed: 40,998,775 bp Total written (filtered): 4,140,609 bp (10.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15449 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 41 0.0 3 0 38 3 20 4424 0.0 4 4190 229 5 21 10384 0.0 4 10148 220 14 2 22 22 0.0 4 5 15 2 23 2 0.0 4 0 0 0 1 1 24 10 0.0 4 1 1 0 7 1 25 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 55 1 0.0 4 1 62 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 1 101 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 1 112 6 0.0 4 0 0 0 0 6 115 3 0.0 4 0 0 0 0 3 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 120 1 0.0 4 1 126 2 0.0 4 0 0 0 0 2 127 421 0.0 4 0 0 0 0 421 128 92 0.0 4 0 0 0 0 92 130 1 0.0 4 0 0 0 0 1 131 2 0.0 4 0 0 0 0 2 134 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 143 2 0.0 4 0 0 0 0 2 145 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 171 4 0.0 4 0 0 0 0 4 172 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 1 229 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 15,408 Reads with adapters: 2,446 (15.9%) Reads written (passing filters): 15,408 (100.0%) Total basepairs processed: 4,140,609 bp Total written (filtered): 4,112,728 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2446 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 3.4% G: 94.3% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 240.8 0 6 4 1 60.2 0 1 6 1 3.8 0 1 9 1350 0.1 0 43 1307 10 492 0.0 1 18 474 11 568 0.0 1 4 564 12 5 0.0 1 0 5 13 1 0.0 1 0 1 14 1 0.0 1 0 1 42 1 0.0 1 1 139 1 0.0 1 1 208 1 0.0 1 0 1 222 1 0.0 1 1 223 16 0.0 1 15 1 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.