This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.51 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 74,281 (3.3%) Reads that were too short: 10,248 (0.5%) Reads written (passing filters): 64,033 (2.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 14,920,730 bp (2.2%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 74281 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 243 546.6 0 243 7 208 546.6 0 208 8 212 546.6 0 212 9 119 546.6 0 119 10 2177 546.6 0 2177 11 35 546.6 0 35 12 62 546.6 0 62 13 11 546.6 0 11 14 16 546.6 0 16 15 10 546.6 0 10 16 14 546.6 0 14 17 6 546.6 0 6 18 34 546.6 0 34 19 18 546.6 0 18 20 20 546.6 0 20 21 46 546.6 0 46 22 32 546.6 0 32 23 21 546.6 0 21 24 17 546.6 0 17 25 30 546.6 0 30 26 24 546.6 0 24 27 26 546.6 0 26 28 73 546.6 0 73 29 18 546.6 0 18 30 28 546.6 0 28 31 23 546.6 0 23 32 18 546.6 0 18 33 17 546.6 0 17 34 28 546.6 0 28 35 17 546.6 0 17 36 17 546.6 0 17 37 20 546.6 0 20 38 85 546.6 0 85 39 23 546.6 0 23 40 90 546.6 0 90 41 49 546.6 0 49 42 52 546.6 0 52 43 114 546.6 0 114 44 19 546.6 0 19 45 20 546.6 0 20 46 17 546.6 0 17 47 40 546.6 0 40 48 90 546.6 0 90 49 84 546.6 0 84 50 124 546.6 0 124 51 267 546.6 0 267 52 312 546.6 0 312 53 521 546.6 0 521 54 1437 546.6 0 1437 55 14389 546.6 0 14389 56 3828 546.6 0 3828 57 25910 546.6 0 25910 58 105 546.6 0 105 59 21 546.6 0 21 60 24 546.6 0 24 61 26 546.6 0 26 62 31 546.6 0 31 63 27 546.6 0 27 64 14 546.6 0 14 65 33 546.6 0 33 66 18 546.6 0 18 67 14 546.6 0 14 68 20 546.6 0 20 69 12 546.6 0 12 70 30 546.6 0 30 71 34 546.6 0 34 72 16 546.6 0 16 73 20 546.6 0 20 74 33 546.6 0 33 75 25 546.6 0 25 76 150 546.6 0 150 77 26 546.6 0 26 78 39 546.6 0 39 79 30 546.6 0 30 80 38 546.6 0 38 81 34 546.6 0 34 82 43 546.6 0 43 83 66 546.6 0 66 84 93 546.6 0 93 85 1084 546.6 0 1084 86 434 546.6 0 434 87 1338 546.6 0 1338 88 51 546.6 0 51 89 70 546.6 0 70 90 38 546.6 0 38 91 22 546.6 0 22 92 23 546.6 0 23 93 21 546.6 0 21 94 42 546.6 0 42 95 18 546.6 0 18 96 16 546.6 0 16 97 33 546.6 0 33 98 26 546.6 0 26 99 42 546.6 0 42 100 73 546.6 0 73 101 63 546.6 0 63 102 536 546.6 0 536 103 120 546.6 0 120 104 148 546.6 0 148 105 29 546.6 0 29 106 22 546.6 0 22 107 32 546.6 0 32 108 22 546.6 0 22 109 32 546.6 0 32 110 22 546.6 0 22 111 26 546.6 0 26 112 22 546.6 0 22 113 22 546.6 0 22 114 17 546.6 0 17 115 26 546.6 0 26 116 43 546.6 0 43 117 36 546.6 0 36 118 612 546.6 0 612 119 90 546.6 0 90 120 145 546.6 0 145 121 26 546.6 0 26 122 20 546.6 0 20 123 27 546.6 0 27 124 35 546.6 0 35 125 24 546.6 0 24 126 29 546.6 0 29 127 24 546.6 0 24 128 40 546.6 0 40 129 34 546.6 0 34 130 52 546.6 0 52 131 33 546.6 0 33 132 55 546.6 0 55 133 83 546.6 0 83 134 60 546.6 0 60 135 51 546.6 0 51 136 62 546.6 0 62 137 134 546.6 0 134 138 171 546.6 0 171 139 381 546.6 0 381 140 173 546.6 0 173 141 126 546.6 0 126 142 96 546.6 0 96 143 31 546.6 0 31 144 53 546.6 0 53 145 28 546.6 0 28 146 43 546.6 0 43 147 57 546.6 0 57 148 60 546.6 0 60 149 41 546.6 0 41 150 41 546.6 0 41 151 31 546.6 0 31 152 40 546.6 0 40 153 49 546.6 0 49 154 56 546.6 0 56 155 30 546.6 0 30 156 36 546.6 0 36 157 234 546.6 0 234 158 130 546.6 0 130 159 314 546.6 0 314 160 37 546.6 0 37 161 47 546.6 0 47 162 36 546.6 0 36 163 55 546.6 0 55 164 67 546.6 0 67 165 115 546.6 0 115 166 156 546.6 0 156 167 105 546.6 0 105 168 143 546.6 0 143 169 139 546.6 0 139 170 79 546.6 0 79 171 66 546.6 0 66 172 143 546.6 0 143 173 31 546.6 0 31 174 44 546.6 0 44 175 36 546.6 0 36 176 37 546.6 0 37 177 36 546.6 0 36 178 31 546.6 0 31 179 30 546.6 0 30 180 42 546.6 0 42 181 42 546.6 0 42 182 55 546.6 0 55 183 87 546.6 0 87 184 115 546.6 0 115 185 137 546.6 0 137 186 47 546.6 0 47 187 247 546.6 0 247 188 233 546.6 0 233 189 419 546.6 0 419 190 170 546.6 0 170 191 52 546.6 0 52 192 41 546.6 0 41 193 18 546.6 0 18 194 44 546.6 0 44 195 20 546.6 0 20 196 28 546.6 0 28 197 23 546.6 0 23 198 38 546.6 0 38 199 79 546.6 0 79 200 71 546.6 0 71 201 53 546.6 0 53 202 36 546.6 0 36 203 36 546.6 0 36 204 35 546.6 0 35 205 47 546.6 0 47 206 67 546.6 0 67 207 92 546.6 0 92 208 72 546.6 0 72 209 25 546.6 0 25 210 24 546.6 0 24 211 32 546.6 0 32 212 33 546.6 0 33 213 51 546.6 0 51 214 43 546.6 0 43 215 59 546.6 0 59 216 39 546.6 0 39 217 40 546.6 0 40 218 75 546.6 0 75 219 430 546.6 0 430 220 155 546.6 0 155 221 236 546.6 0 236 222 17 546.6 0 17 223 42 546.6 0 42 224 48 546.6 0 48 225 54 546.6 0 54 226 35 546.6 0 35 227 23 546.6 0 23 228 20 546.6 0 20 229 32 546.6 0 32 230 37 546.6 0 37 231 41 546.6 0 41 232 23 546.6 0 23 233 38 546.6 0 38 234 47 546.6 0 47 235 102 546.6 0 102 236 141 546.6 0 141 237 52 546.6 0 52 238 45 546.6 0 45 239 83 546.6 0 83 240 117 546.6 0 117 241 79 546.6 0 79 242 59 546.6 0 59 243 40 546.6 0 40 244 31 546.6 0 31 245 29 546.6 0 29 246 47 546.6 0 47 247 48 546.6 0 48 248 43 546.6 0 43 249 71 546.6 0 71 250 75 546.6 0 75 251 97 546.6 0 97 252 34 546.6 0 34 253 41 546.6 0 41 254 26 546.6 0 26 255 36 546.6 0 36 256 30 546.6 0 30 257 46 546.6 0 46 258 80 546.6 0 80 259 152 546.6 0 152 260 138 546.6 0 138 261 156 546.6 0 156 262 197 546.6 0 197 263 38 546.6 0 38 264 32 546.6 0 32 265 41 546.6 0 41 266 61 546.6 0 61 267 63 546.6 0 63 268 64 546.6 0 64 269 112 546.6 0 112 270 47 546.6 0 47 271 35 546.6 0 35 272 66 546.6 0 66 273 52 546.6 0 52 274 101 546.6 0 101 275 79 546.6 0 79 276 316 546.6 0 316 277 57 546.6 0 57 278 445 546.6 0 445 279 141 546.6 0 141 280 298 546.6 0 298 281 143 546.6 0 143 282 98 546.6 0 98 283 206 546.6 0 206 284 78 546.6 0 78 285 76 546.6 0 76 286 69 546.6 0 69 287 115 546.6 0 115 288 284 546.6 0 284 289 265 546.6 0 265 290 82 546.6 0 82 291 90 546.6 0 90 292 114 546.6 0 114 293 111 546.6 0 111 294 113 546.6 0 113 295 140 546.6 0 140 296 214 546.6 0 214 297 136 546.6 0 136 298 330 546.6 0 330 299 435 546.6 0 435 300 809 546.6 0 809 301 113 546.6 0 113 Finished in 2.62 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 64,033 Reads with adapters: 395 (0.6%) Reads that were too short: 86 (0.1%) Reads written (passing filters): 309 (0.5%) Total basepairs processed: 14,920,730 bp Total written (filtered): 66,263 bp (0.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 395 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 1 17 177 0.0 3 161 12 3 1 18 6 0.0 3 3 1 1 1 19 2 0.0 3 0 2 20 1 0.0 3 1 21 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 30 2 0.0 3 0 0 1 1 34 32 0.0 3 0 0 0 32 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 12 0.0 3 0 0 0 12 40 5 0.0 3 0 0 0 5 43 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 57 5 0.0 3 0 0 0 5 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 124 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 146 7 0.0 3 0 0 0 7 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 3 0.0 3 0 0 0 3 150 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 161 5 0.0 3 0 0 0 5 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 1 175 1 0.0 3 0 0 0 1 176 4 0.0 3 0 0 0 4 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 16 0.0 3 0 0 0 16 182 13 0.0 3 0 0 0 13 183 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 2 0.0 3 0 0 0 2 233 3 0.0 3 0 0 0 3 234 4 0.0 3 0 0 0 4 235 4 0.0 3 0 0 0 4 236 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 309 Reads with adapters: 5 (1.6%) Reads written (passing filters): 309 (100.0%) Total basepairs processed: 66,263 bp Total written (filtered): 66,248 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.0% C: 20.0% G: 20.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 4.8 0 5