This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 57.50 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 72,722 (3.2%) Reads that were too short: 7,089 (0.3%) Reads written (passing filters): 65,633 (2.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 15,752,426 bp (2.3%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 72722 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 256 546.6 0 256 7 163 546.6 0 163 8 241 546.6 0 241 9 80 546.6 0 80 10 1970 546.6 0 1970 11 21 546.6 0 21 12 43 546.6 0 43 13 10 546.6 0 10 14 17 546.6 0 17 15 10 546.6 0 10 16 9 546.6 0 9 17 10 546.6 0 10 18 34 546.6 0 34 19 19 546.6 0 19 20 18 546.6 0 18 21 26 546.6 0 26 22 13 546.6 0 13 23 13 546.6 0 13 24 13 546.6 0 13 25 17 546.6 0 17 26 21 546.6 0 21 27 28 546.6 0 28 28 67 546.6 0 67 29 16 546.6 0 16 30 13 546.6 0 13 31 16 546.6 0 16 32 13 546.6 0 13 33 11 546.6 0 11 34 20 546.6 0 20 35 29 546.6 0 29 36 12 546.6 0 12 37 31 546.6 0 31 38 108 546.6 0 108 39 33 546.6 0 33 40 87 546.6 0 87 41 40 546.6 0 40 42 54 546.6 0 54 43 156 546.6 0 156 44 26 546.6 0 26 45 22 546.6 0 22 46 23 546.6 0 23 47 26 546.6 0 26 48 89 546.6 0 89 49 58 546.6 0 58 50 121 546.6 0 121 51 293 546.6 0 293 52 324 546.6 0 324 53 530 546.6 0 530 54 1574 546.6 0 1574 55 16548 546.6 0 16548 56 4057 546.6 0 4057 57 30677 546.6 0 30677 58 112 546.6 0 112 59 18 546.6 0 18 60 18 546.6 0 18 61 18 546.6 0 18 62 39 546.6 0 39 63 15 546.6 0 15 64 13 546.6 0 13 65 19 546.6 0 19 66 13 546.6 0 13 67 10 546.6 0 10 68 16 546.6 0 16 69 11 546.6 0 11 70 14 546.6 0 14 71 12 546.6 0 12 72 14 546.6 0 14 73 15 546.6 0 15 74 9 546.6 0 9 75 13 546.6 0 13 76 93 546.6 0 93 77 12 546.6 0 12 78 19 546.6 0 19 79 30 546.6 0 30 80 15 546.6 0 15 81 21 546.6 0 21 82 26 546.6 0 26 83 42 546.6 0 42 84 60 546.6 0 60 85 749 546.6 0 749 86 484 546.6 0 484 87 1364 546.6 0 1364 88 10 546.6 0 10 89 12 546.6 0 12 90 15 546.6 0 15 91 18 546.6 0 18 92 9 546.6 0 9 93 13 546.6 0 13 94 37 546.6 0 37 95 9 546.6 0 9 96 15 546.6 0 15 97 10 546.6 0 10 98 16 546.6 0 16 99 18 546.6 0 18 100 35 546.6 0 35 101 17 546.6 0 17 102 157 546.6 0 157 103 45 546.6 0 45 104 39 546.6 0 39 105 24 546.6 0 24 106 19 546.6 0 19 107 11 546.6 0 11 108 15 546.6 0 15 109 15 546.6 0 15 110 29 546.6 0 29 111 9 546.6 0 9 112 11 546.6 0 11 113 8 546.6 0 8 114 14 546.6 0 14 115 10 546.6 0 10 116 18 546.6 0 18 117 22 546.6 0 22 118 533 546.6 0 533 119 75 546.6 0 75 120 91 546.6 0 91 121 15 546.6 0 15 122 13 546.6 0 13 123 11 546.6 0 11 124 18 546.6 0 18 125 8 546.6 0 8 126 9 546.6 0 9 127 17 546.6 0 17 128 18 546.6 0 18 129 23 546.6 0 23 130 36 546.6 0 36 131 11 546.6 0 11 132 18 546.6 0 18 133 25 546.6 0 25 134 24 546.6 0 24 135 22 546.6 0 22 136 34 546.6 0 34 137 42 546.6 0 42 138 71 546.6 0 71 139 111 546.6 0 111 140 35 546.6 0 35 141 42 546.6 0 42 142 32 546.6 0 32 143 20 546.6 0 20 144 18 546.6 0 18 145 15 546.6 0 15 146 17 546.6 0 17 147 53 546.6 0 53 148 34 546.6 0 34 149 22 546.6 0 22 150 21 546.6 0 21 151 20 546.6 0 20 152 29 546.6 0 29 153 10 546.6 0 10 154 23 546.6 0 23 155 22 546.6 0 22 156 15 546.6 0 15 157 77 546.6 0 77 158 60 546.6 0 60 159 140 546.6 0 140 160 17 546.6 0 17 161 23 546.6 0 23 162 18 546.6 0 18 163 29 546.6 0 29 164 38 546.6 0 38 165 47 546.6 0 47 166 74 546.6 0 74 167 44 546.6 0 44 168 67 546.6 0 67 169 61 546.6 0 61 170 22 546.6 0 22 171 30 546.6 0 30 172 27 546.6 0 27 173 27 546.6 0 27 174 14 546.6 0 14 175 20 546.6 0 20 176 12 546.6 0 12 177 28 546.6 0 28 178 18 546.6 0 18 179 11 546.6 0 11 180 17 546.6 0 17 181 26 546.6 0 26 182 24 546.6 0 24 183 31 546.6 0 31 184 46 546.6 0 46 185 119 546.6 0 119 186 19 546.6 0 19 187 112 546.6 0 112 188 87 546.6 0 87 189 187 546.6 0 187 190 73 546.6 0 73 191 43 546.6 0 43 192 28 546.6 0 28 193 12 546.6 0 12 194 16 546.6 0 16 195 7 546.6 0 7 196 7 546.6 0 7 197 17 546.6 0 17 198 15 546.6 0 15 199 54 546.6 0 54 200 25 546.6 0 25 201 31 546.6 0 31 202 9 546.6 0 9 203 13 546.6 0 13 204 14 546.6 0 14 205 18 546.6 0 18 206 24 546.6 0 24 207 21 546.6 0 21 208 69 546.6 0 69 209 20 546.6 0 20 210 13 546.6 0 13 211 13 546.6 0 13 212 15 546.6 0 15 213 21 546.6 0 21 214 15 546.6 0 15 215 14 546.6 0 14 216 9 546.6 0 9 217 17 546.6 0 17 218 22 546.6 0 22 219 258 546.6 0 258 220 82 546.6 0 82 221 116 546.6 0 116 222 6 546.6 0 6 223 5 546.6 0 5 224 22 546.6 0 22 225 21 546.6 0 21 226 17 546.6 0 17 227 14 546.6 0 14 228 8 546.6 0 8 229 21 546.6 0 21 230 15 546.6 0 15 231 23 546.6 0 23 232 9 546.6 0 9 233 17 546.6 0 17 234 21 546.6 0 21 235 36 546.6 0 36 236 43 546.6 0 43 237 25 546.6 0 25 238 23 546.6 0 23 239 26 546.6 0 26 240 42 546.6 0 42 241 38 546.6 0 38 242 12 546.6 0 12 243 22 546.6 0 22 244 15 546.6 0 15 245 15 546.6 0 15 246 13 546.6 0 13 247 27 546.6 0 27 248 15 546.6 0 15 249 25 546.6 0 25 250 35 546.6 0 35 251 35 546.6 0 35 252 9 546.6 0 9 253 11 546.6 0 11 254 11 546.6 0 11 255 14 546.6 0 14 256 14 546.6 0 14 257 17 546.6 0 17 258 34 546.6 0 34 259 69 546.6 0 69 260 55 546.6 0 55 261 60 546.6 0 60 262 77 546.6 0 77 263 18 546.6 0 18 264 11 546.6 0 11 265 17 546.6 0 17 266 23 546.6 0 23 267 28 546.6 0 28 268 24 546.6 0 24 269 22 546.6 0 22 270 26 546.6 0 26 271 22 546.6 0 22 272 38 546.6 0 38 273 27 546.6 0 27 274 47 546.6 0 47 275 66 546.6 0 66 276 613 546.6 0 613 277 67 546.6 0 67 278 1009 546.6 0 1009 279 55 546.6 0 55 280 243 546.6 0 243 281 70 546.6 0 70 282 47 546.6 0 47 283 48 546.6 0 48 284 40 546.6 0 40 285 36 546.6 0 36 286 35 546.6 0 35 287 54 546.6 0 54 288 118 546.6 0 118 289 107 546.6 0 107 290 50 546.6 0 50 291 55 546.6 0 55 292 46 546.6 0 46 293 59 546.6 0 59 294 46 546.6 0 46 295 59 546.6 0 59 296 93 546.6 0 93 297 81 546.6 0 81 298 370 546.6 0 370 299 546 546.6 0 546 300 924 546.6 0 924 301 49 546.6 0 49 Finished in 3.03 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 65,633 Reads with adapters: 513 (0.8%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 508 (0.8%) Total basepairs processed: 15,752,426 bp Total written (filtered): 131,755 bp (0.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 513 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 1 0.0 3 0 1 20 212 0.0 4 204 8 22 1 0.0 4 0 0 1 23 231 0.0 4 230 1 24 1 0.0 4 1 30 1 0.0 4 1 74 1 0.0 4 1 75 1 0.0 4 1 88 1 0.0 4 1 114 1 0.0 4 0 1 118 1 0.0 4 1 123 1 0.0 4 0 0 0 0 1 124 55 0.0 4 0 0 0 0 55 135 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 165 1 0.0 4 1 210 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 508 Reads with adapters: 75 (14.8%) Reads written (passing filters): 508 (100.0%) Total basepairs processed: 131,755 bp Total written (filtered): 130,766 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 75 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 2.7% G: 90.7% T: 5.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 6 1 0.1 0 1 9 12 0.0 0 0 12 10 19 0.0 1 4 15 11 42 0.0 1 1 41 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.