This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.23 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 102,874 (3.6%) Reads that were too short: 12,205 (0.4%) Reads written (passing filters): 90,669 (3.2%) Total basepairs processed: 853,219,115 bp Total written (filtered): 18,529,793 bp (2.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 102874 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 581 692.0 0 581 7 13770 692.0 0 13770 8 11133 692.0 0 11133 9 92 692.0 0 92 10 73 692.0 0 73 11 44 692.0 0 44 12 34 692.0 0 34 13 39 692.0 0 39 14 63 692.0 0 63 15 60 692.0 0 60 16 55 692.0 0 55 17 50 692.0 0 50 18 61 692.0 0 61 19 43 692.0 0 43 20 54 692.0 0 54 21 53 692.0 0 53 22 46 692.0 0 46 23 57 692.0 0 57 24 42 692.0 0 42 25 44 692.0 0 44 26 66 692.0 0 66 27 231 692.0 0 231 28 328 692.0 0 328 29 463 692.0 0 463 30 42 692.0 0 42 31 36 692.0 0 36 32 43 692.0 0 43 33 39 692.0 0 39 34 43 692.0 0 43 35 66 692.0 0 66 36 67 692.0 0 67 37 50 692.0 0 50 38 52 692.0 0 52 39 56 692.0 0 56 40 32 692.0 0 32 41 57 692.0 0 57 42 46 692.0 0 46 43 46 692.0 0 46 44 53 692.0 0 53 45 55 692.0 0 55 46 47 692.0 0 47 47 42 692.0 0 42 48 47 692.0 0 47 49 45 692.0 0 45 50 70 692.0 0 70 51 53 692.0 0 53 52 67 692.0 0 67 53 47 692.0 0 47 54 58 692.0 0 58 55 60 692.0 0 60 56 104 692.0 0 104 57 99 692.0 0 99 58 673 692.0 0 673 59 257 692.0 0 257 60 352 692.0 0 352 61 37 692.0 0 37 62 52 692.0 0 52 63 35 692.0 0 35 64 45 692.0 0 45 65 44 692.0 0 44 66 44 692.0 0 44 67 49 692.0 0 49 68 59 692.0 0 59 69 80 692.0 0 80 70 146 692.0 0 146 71 144 692.0 0 144 72 1350 692.0 0 1350 73 366 692.0 0 366 74 863 692.0 0 863 75 59 692.0 0 59 76 57 692.0 0 57 77 61 692.0 0 61 78 60 692.0 0 60 79 62 692.0 0 62 80 64 692.0 0 64 81 80 692.0 0 80 82 39 692.0 0 39 83 46 692.0 0 46 84 61 692.0 0 61 85 49 692.0 0 49 86 44 692.0 0 44 87 48 692.0 0 48 88 101 692.0 0 101 89 57 692.0 0 57 90 146 692.0 0 146 91 108 692.0 0 108 92 289 692.0 0 289 93 56 692.0 0 56 94 47 692.0 0 47 95 81 692.0 0 81 96 63 692.0 0 63 97 41 692.0 0 41 98 106 692.0 0 106 99 67 692.0 0 67 100 41 692.0 0 41 101 67 692.0 0 67 102 42 692.0 0 42 103 46 692.0 0 46 104 50 692.0 0 50 105 40 692.0 0 40 106 42 692.0 0 42 107 53 692.0 0 53 108 88 692.0 0 88 109 100 692.0 0 100 110 110 692.0 0 110 111 61 692.0 0 61 112 385 692.0 0 385 113 222 692.0 0 222 114 1115 692.0 0 1115 115 41 692.0 0 41 116 57 692.0 0 57 117 78 692.0 0 78 118 51 692.0 0 51 119 102 692.0 0 102 120 81 692.0 0 81 121 86 692.0 0 86 122 57 692.0 0 57 123 63 692.0 0 63 124 67 692.0 0 67 125 77 692.0 0 77 126 105 692.0 0 105 127 99 692.0 0 99 128 157 692.0 0 157 129 280 692.0 0 280 130 504 692.0 0 504 131 233 692.0 0 233 132 148 692.0 0 148 133 325 692.0 0 325 134 477 692.0 0 477 135 158 692.0 0 158 136 212 692.0 0 212 137 267 692.0 0 267 138 205 692.0 0 205 139 219 692.0 0 219 140 325 692.0 0 325 141 528 692.0 0 528 142 607 692.0 0 607 143 6207 692.0 0 6207 144 5592 692.0 0 5592 145 28557 692.0 0 28557 146 213 692.0 0 213 147 93 692.0 0 93 148 92 692.0 0 92 149 110 692.0 0 110 150 182 692.0 0 182 151 43 692.0 0 43 152 53 692.0 0 53 153 56 692.0 0 56 154 61 692.0 0 61 155 182 692.0 0 182 156 420 692.0 0 420 157 710 692.0 0 710 158 61 692.0 0 61 159 70 692.0 0 70 160 64 692.0 0 64 161 73 692.0 0 73 162 81 692.0 0 81 163 460 692.0 0 460 164 1013 692.0 0 1013 165 612 692.0 0 612 166 63 692.0 0 63 167 55 692.0 0 55 168 68 692.0 0 68 169 82 692.0 0 82 170 59 692.0 0 59 171 45 692.0 0 45 172 39 692.0 0 39 173 52 692.0 0 52 174 43 692.0 0 43 175 66 692.0 0 66 176 50 692.0 0 50 177 58 692.0 0 58 178 49 692.0 0 49 179 53 692.0 0 53 180 54 692.0 0 54 181 45 692.0 0 45 182 57 692.0 0 57 183 56 692.0 0 56 184 63 692.0 0 63 185 69 692.0 0 69 186 70 692.0 0 70 187 52 692.0 0 52 188 68 692.0 0 68 189 44 692.0 0 44 190 66 692.0 0 66 191 58 692.0 0 58 192 54 692.0 0 54 193 73 692.0 0 73 194 48 692.0 0 48 195 58 692.0 0 58 196 89 692.0 0 89 197 60 692.0 0 60 198 56 692.0 0 56 199 37 692.0 0 37 200 51 692.0 0 51 201 91 692.0 0 91 202 70 692.0 0 70 203 116 692.0 0 116 204 85 692.0 0 85 205 68 692.0 0 68 206 79 692.0 0 79 207 74 692.0 0 74 208 116 692.0 0 116 209 165 692.0 0 165 210 164 692.0 0 164 211 192 692.0 0 192 212 102 692.0 0 102 213 263 692.0 0 263 214 350 692.0 0 350 215 766 692.0 0 766 216 372 692.0 0 372 217 408 692.0 0 408 218 68 692.0 0 68 219 49 692.0 0 49 220 56 692.0 0 56 221 111 692.0 0 111 222 84 692.0 0 84 223 131 692.0 0 131 224 68 692.0 0 68 225 77 692.0 0 77 226 102 692.0 0 102 227 74 692.0 0 74 228 69 692.0 0 69 229 69 692.0 0 69 230 73 692.0 0 73 231 276 692.0 0 276 232 246 692.0 0 246 233 660 692.0 0 660 234 149 692.0 0 149 235 108 692.0 0 108 236 166 692.0 0 166 237 71 692.0 0 71 238 43 692.0 0 43 239 57 692.0 0 57 240 66 692.0 0 66 241 48 692.0 0 48 242 127 692.0 0 127 243 73 692.0 0 73 244 111 692.0 0 111 245 109 692.0 0 109 246 67 692.0 0 67 247 54 692.0 0 54 248 55 692.0 0 55 249 68 692.0 0 68 250 46 692.0 0 46 251 50 692.0 0 50 252 58 692.0 0 58 253 85 692.0 0 85 254 78 692.0 0 78 255 83 692.0 0 83 256 53 692.0 0 53 257 49 692.0 0 49 258 72 692.0 0 72 259 67 692.0 0 67 260 84 692.0 0 84 261 131 692.0 0 131 262 66 692.0 0 66 263 113 692.0 0 113 264 150 692.0 0 150 265 208 692.0 0 208 266 89 692.0 0 89 267 63 692.0 0 63 268 53 692.0 0 53 269 89 692.0 0 89 270 104 692.0 0 104 271 114 692.0 0 114 272 74 692.0 0 74 273 68 692.0 0 68 274 67 692.0 0 67 275 82 692.0 0 82 276 59 692.0 0 59 277 80 692.0 0 80 278 88 692.0 0 88 279 84 692.0 0 84 280 92 692.0 0 92 281 90 692.0 0 90 282 178 692.0 0 178 283 103 692.0 0 103 284 132 692.0 0 132 285 106 692.0 0 106 286 139 692.0 0 139 287 76 692.0 0 76 288 62 692.0 0 62 289 73 692.0 0 73 290 81 692.0 0 81 291 104 692.0 0 104 292 104 692.0 0 104 293 110 692.0 0 110 294 104 692.0 0 104 295 175 692.0 0 175 296 334 692.0 0 334 297 284 692.0 0 284 298 141 692.0 0 141 299 83 692.0 0 83 300 86 692.0 0 86 301 66 692.0 0 66 Finished in 3.59 s (40 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 90,669 Reads with adapters: 13,663 (15.1%) Reads that were too short: 203 (0.2%) Reads written (passing filters): 13,460 (14.8%) Total basepairs processed: 18,529,793 bp Total written (filtered): 3,684,850 bp (19.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13663 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 11 0.0 3 0 0 5 6 16 135 0.0 3 0 114 16 5 17 12824 0.0 3 11839 819 140 26 18 142 0.0 3 2 21 5 114 19 46 0.0 3 4 1 15 26 20 36 0.0 3 0 0 0 36 21 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 27 2 0.0 3 0 0 0 2 28 49 0.0 3 0 0 0 49 29 1 0.0 3 0 0 0 1 31 7 0.0 3 0 0 1 6 33 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 47 1 0.0 3 1 48 64 0.0 3 0 0 2 62 49 4 0.0 3 0 0 0 4 51 15 0.0 3 0 0 1 14 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 59 6 0.0 3 0 0 0 6 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 6 0.0 3 0 0 0 6 75 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 1 2 79 37 0.0 3 0 0 1 36 82 8 0.0 3 0 0 0 8 85 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 90 3 0.0 3 0 0 0 3 96 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 10 0.0 3 0 0 1 9 100 9 0.0 3 0 0 1 8 101 4 0.0 3 0 0 0 4 102 4 0.0 3 0 0 0 4 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 116 2 0.0 3 0 1 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 129 2 0.0 3 0 0 1 1 130 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 19 0.0 3 0 0 0 19 137 1 0.0 3 0 0 0 1 138 3 0.0 3 0 0 0 3 139 3 0.0 3 0 0 0 3 140 4 0.0 3 0 0 0 4 141 3 0.0 3 0 0 0 3 143 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 15 0.0 3 0 0 1 14 150 70 0.0 3 0 0 5 65 152 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 21 0.0 3 0 0 0 21 165 2 0.0 3 0 0 0 2 167 9 0.0 3 0 0 0 9 178 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 181 4 0.0 3 0 0 0 4 188 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 214 3 0.0 3 0 0 0 3 215 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 13,460 Reads with adapters: 459 (3.4%) Reads written (passing filters): 13,460 (100.0%) Total basepairs processed: 3,684,850 bp Total written (filtered): 3,683,471 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 459 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.3% C: 66.7% G: 19.6% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 458 210.3 0 458 5 1 13.1 0 1