This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.30 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 223,853 (7.9%) Reads that were too short: 20,509 (0.7%) Reads written (passing filters): 203,344 (7.2%) Total basepairs processed: 853,219,115 bp Total written (filtered): 38,212,275 bp (4.5%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 223853 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 116 692.0 0 116 7 4587 692.0 0 4587 8 4198 692.0 0 4198 9 33 692.0 0 33 10 18 692.0 0 18 11 42 692.0 0 42 12 389 692.0 0 389 13 99 692.0 0 99 14 412 692.0 0 412 15 31 692.0 0 31 16 22 692.0 0 22 17 33 692.0 0 33 18 36 692.0 0 36 19 18 692.0 0 18 20 39 692.0 0 39 21 36 692.0 0 36 22 40 692.0 0 40 23 39 692.0 0 39 24 56 692.0 0 56 25 335 692.0 0 335 26 116 692.0 0 116 27 117 692.0 0 117 28 31 692.0 0 31 29 34 692.0 0 34 30 113 692.0 0 113 31 31 692.0 0 31 32 52 692.0 0 52 33 32 692.0 0 32 34 42 692.0 0 42 35 48 692.0 0 48 36 55 692.0 0 55 37 84 692.0 0 84 38 43 692.0 0 43 39 48 692.0 0 48 40 55 692.0 0 55 41 43 692.0 0 43 42 81 692.0 0 81 43 90 692.0 0 90 44 95 692.0 0 95 45 30 692.0 0 30 46 53 692.0 0 53 47 39 692.0 0 39 48 43 692.0 0 43 49 57 692.0 0 57 50 44 692.0 0 44 51 94 692.0 0 94 52 46 692.0 0 46 53 43 692.0 0 43 54 63 692.0 0 63 55 78 692.0 0 78 56 116 692.0 0 116 57 50 692.0 0 50 58 47 692.0 0 47 59 35 692.0 0 35 60 71 692.0 0 71 61 47 692.0 0 47 62 84 692.0 0 84 63 100 692.0 0 100 64 68 692.0 0 68 65 574 692.0 0 574 66 273 692.0 0 273 67 482 692.0 0 482 68 62 692.0 0 62 69 38 692.0 0 38 70 58 692.0 0 58 71 66 692.0 0 66 72 79 692.0 0 79 73 71 692.0 0 71 74 62 692.0 0 62 75 44 692.0 0 44 76 32 692.0 0 32 77 89 692.0 0 89 78 122 692.0 0 122 79 98 692.0 0 98 80 193 692.0 0 193 81 133 692.0 0 133 82 98 692.0 0 98 83 77 692.0 0 77 84 94 692.0 0 94 85 63 692.0 0 63 86 92 692.0 0 92 87 161 692.0 0 161 88 215 692.0 0 215 89 369 692.0 0 369 90 740 692.0 0 740 91 1289 692.0 0 1289 92 345 692.0 0 345 93 367 692.0 0 367 94 786 692.0 0 786 95 340 692.0 0 340 96 333 692.0 0 333 97 494 692.0 0 494 98 589 692.0 0 589 99 545 692.0 0 545 100 622 692.0 0 622 101 904 692.0 0 904 102 1377 692.0 0 1377 103 1858 692.0 0 1858 104 30814 692.0 0 30814 105 29494 692.0 0 29494 106 59802 692.0 0 59802 107 271 692.0 0 271 108 61 692.0 0 61 109 34 692.0 0 34 110 79 692.0 0 79 111 81 692.0 0 81 112 116 692.0 0 116 113 79 692.0 0 79 114 102 692.0 0 102 115 424 692.0 0 424 116 116 692.0 0 116 117 1080 692.0 0 1080 118 81 692.0 0 81 119 190 692.0 0 190 120 75 692.0 0 75 121 167 692.0 0 167 122 71 692.0 0 71 123 121 692.0 0 121 124 1139 692.0 0 1139 125 109 692.0 0 109 126 239 692.0 0 239 127 96 692.0 0 96 128 62 692.0 0 62 129 68 692.0 0 68 130 71 692.0 0 71 131 50 692.0 0 50 132 155 692.0 0 155 133 53 692.0 0 53 134 68 692.0 0 68 135 62 692.0 0 62 136 60 692.0 0 60 137 39 692.0 0 39 138 177 692.0 0 177 139 118 692.0 0 118 140 127 692.0 0 127 141 83 692.0 0 83 142 165 692.0 0 165 143 64 692.0 0 64 144 141 692.0 0 141 145 116 692.0 0 116 146 154 692.0 0 154 147 121 692.0 0 121 148 117 692.0 0 117 149 105 692.0 0 105 150 65 692.0 0 65 151 194 692.0 0 194 152 169 692.0 0 169 153 314 692.0 0 314 154 263 692.0 0 263 155 244 692.0 0 244 156 334 692.0 0 334 157 710 692.0 0 710 158 1197 692.0 0 1197 159 20606 692.0 0 20606 160 9874 692.0 0 9874 161 9098 692.0 0 9098 162 106 692.0 0 106 163 67 692.0 0 67 164 63 692.0 0 63 165 60 692.0 0 60 166 72 692.0 0 72 167 384 692.0 0 384 168 156 692.0 0 156 169 269 692.0 0 269 170 53 692.0 0 53 171 98 692.0 0 98 172 229 692.0 0 229 173 259 692.0 0 259 174 133 692.0 0 133 175 82 692.0 0 82 176 51 692.0 0 51 177 68 692.0 0 68 178 69 692.0 0 69 179 94 692.0 0 94 180 73 692.0 0 73 181 77 692.0 0 77 182 156 692.0 0 156 183 99 692.0 0 99 184 280 692.0 0 280 185 166 692.0 0 166 186 168 692.0 0 168 187 120 692.0 0 120 188 117 692.0 0 117 189 143 692.0 0 143 190 62 692.0 0 62 191 96 692.0 0 96 192 70 692.0 0 70 193 62 692.0 0 62 194 97 692.0 0 97 195 70 692.0 0 70 196 116 692.0 0 116 197 98 692.0 0 98 198 192 692.0 0 192 199 211 692.0 0 211 200 860 692.0 0 860 201 382 692.0 0 382 202 286 692.0 0 286 203 69 692.0 0 69 204 59 692.0 0 59 205 123 692.0 0 123 206 135 692.0 0 135 207 142 692.0 0 142 208 89 692.0 0 89 209 171 692.0 0 171 210 65 692.0 0 65 211 66 692.0 0 66 212 77 692.0 0 77 213 97 692.0 0 97 214 183 692.0 0 183 215 92 692.0 0 92 216 82 692.0 0 82 217 101 692.0 0 101 218 189 692.0 0 189 219 452 692.0 0 452 220 239 692.0 0 239 221 282 692.0 0 282 222 70 692.0 0 70 223 90 692.0 0 90 224 155 692.0 0 155 225 70 692.0 0 70 226 175 692.0 0 175 227 101 692.0 0 101 228 276 692.0 0 276 229 145 692.0 0 145 230 236 692.0 0 236 231 106 692.0 0 106 232 93 692.0 0 93 233 108 692.0 0 108 234 113 692.0 0 113 235 72 692.0 0 72 236 115 692.0 0 115 237 108 692.0 0 108 238 227 692.0 0 227 239 221 692.0 0 221 240 1677 692.0 0 1677 241 1297 692.0 0 1297 242 1272 692.0 0 1272 243 279 692.0 0 279 244 95 692.0 0 95 245 71 692.0 0 71 246 106 692.0 0 106 247 109 692.0 0 109 248 135 692.0 0 135 249 84 692.0 0 84 250 98 692.0 0 98 251 90 692.0 0 90 252 109 692.0 0 109 253 123 692.0 0 123 254 306 692.0 0 306 255 312 692.0 0 312 256 265 692.0 0 265 257 182 692.0 0 182 258 131 692.0 0 131 259 117 692.0 0 117 260 91 692.0 0 91 261 97 692.0 0 97 262 66 692.0 0 66 263 89 692.0 0 89 264 85 692.0 0 85 265 104 692.0 0 104 266 116 692.0 0 116 267 222 692.0 0 222 268 194 692.0 0 194 269 182 692.0 0 182 270 156 692.0 0 156 271 102 692.0 0 102 272 100 692.0 0 100 273 116 692.0 0 116 274 203 692.0 0 203 275 173 692.0 0 173 276 264 692.0 0 264 277 151 692.0 0 151 278 253 692.0 0 253 279 302 692.0 0 302 280 408 692.0 0 408 281 402 692.0 0 402 282 431 692.0 0 431 283 1116 692.0 0 1116 284 157 692.0 0 157 285 80 692.0 0 80 286 96 692.0 0 96 287 102 692.0 0 102 288 180 692.0 0 180 289 125 692.0 0 125 290 126 692.0 0 126 291 105 692.0 0 105 292 143 692.0 0 143 293 168 692.0 0 168 294 226 692.0 0 226 295 170 692.0 0 170 296 154 692.0 0 154 297 78 692.0 0 78 298 84 692.0 0 84 299 147 692.0 0 147 300 423 692.0 0 423 301 184 692.0 0 184 Finished in 7.08 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 203,344 Reads with adapters: 5,518 (2.7%) Reads that were too short: 413 (0.2%) Reads written (passing filters): 5,105 (2.5%) Total basepairs processed: 38,212,275 bp Total written (filtered): 1,312,518 bp (3.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5518 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 2 0.0 3 0 0 1 1 16 47 0.0 3 0 41 4 2 17 4390 0.0 3 4034 293 49 14 18 19 0.0 3 1 7 1 10 19 12 0.0 3 0 0 3 9 20 13 0.0 3 1 1 0 11 21 1 0.0 3 0 0 1 22 2 0.0 3 0 0 0 2 23 21 0.0 3 0 0 0 21 24 1 0.0 3 0 0 0 1 28 6 0.0 3 0 0 0 6 32 1 0.0 3 0 0 0 1 33 1 0.0 3 1 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 42 2 0.0 3 1 0 0 1 44 30 0.0 3 0 1 0 29 45 2 0.0 3 0 0 1 1 46 47 0.0 3 0 0 0 47 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 54 4 0.0 3 0 0 0 4 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 18 0.0 3 0 0 0 18 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 1 0 1 62 3 0.0 3 0 0 0 3 63 1 0.0 3 0 1 65 8 0.0 3 0 0 0 8 66 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 69 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 71 3 0.0 3 0 0 0 3 72 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 0 3 75 1 0.0 3 0 0 0 1 78 10 0.0 3 0 0 0 10 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 86 5 0.0 3 0 0 0 5 87 167 0.0 3 0 0 4 163 88 155 0.0 3 0 0 1 154 89 2 0.0 3 0 0 0 2 90 40 0.0 3 0 0 1 39 91 26 0.0 3 0 0 1 25 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 1 95 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 5 0.0 3 0 0 1 4 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 4 0.0 3 0 0 1 3 108 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 0 3 110 3 0.0 3 1 0 0 2 111 9 0.0 3 0 0 0 9 112 1 0.0 3 0 0 0 1 113 11 0.0 3 0 0 0 11 114 14 0.0 3 0 0 2 12 117 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 4 0.0 3 0 0 0 4 125 3 0.0 3 0 0 0 3 126 21 0.0 3 0 0 1 20 127 1 0.0 3 0 0 0 1 128 5 0.0 3 0 0 0 5 129 15 0.0 3 0 0 1 14 130 13 0.0 3 0 0 0 13 131 9 0.0 3 0 0 0 9 132 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 4 0.0 3 0 0 0 4 138 18 0.0 3 0 0 1 17 139 9 0.0 3 0 0 0 9 140 2 0.0 3 0 0 0 2 141 7 0.0 3 0 0 0 7 142 8 0.0 3 0 0 0 8 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 4 0.0 3 0 0 0 4 147 8 0.0 3 0 0 0 8 148 6 0.0 3 0 0 0 6 149 3 0.0 3 0 0 0 3 150 2 0.0 3 0 0 0 2 152 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 166 6 0.0 3 0 0 0 6 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 2 0.0 3 0 0 0 2 170 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 1 1 178 2 0.0 3 0 0 0 2 179 4 0.0 3 0 0 0 4 180 3 0.0 3 0 0 0 3 181 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 188 40 0.0 3 0 0 6 34 189 105 0.0 3 0 0 8 97 190 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 1 199 2 0.0 3 0 0 0 2 200 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 250 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 5,105 Reads with adapters: 15 (0.3%) Reads written (passing filters): 5,105 (100.0%) Total basepairs processed: 1,312,518 bp Total written (filtered): 1,312,472 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 15 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.7% C: 13.3% G: 13.3% T: 26.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 14 79.8 0 14 4 1 19.9 0 1