This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 161.79 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 293,987 (8.5%) Reads that were too short: 51,488 (1.5%) Reads written (passing filters): 242,499 (7.0%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 53,291,862 bp (5.1%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 293987 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1475 847.3 0 1475 7 48454 847.3 0 48454 8 37370 847.3 0 37370 9 94 847.3 0 94 10 47 847.3 0 47 11 122 847.3 0 122 12 59 847.3 0 59 13 53 847.3 0 53 14 39 847.3 0 39 15 31 847.3 0 31 16 51 847.3 0 51 17 49 847.3 0 49 18 50 847.3 0 50 19 44 847.3 0 44 20 77 847.3 0 77 21 45 847.3 0 45 22 53 847.3 0 53 23 40 847.3 0 40 24 64 847.3 0 64 25 34 847.3 0 34 26 48 847.3 0 48 27 80 847.3 0 80 28 66 847.3 0 66 29 48 847.3 0 48 30 64 847.3 0 64 31 75 847.3 0 75 32 53 847.3 0 53 33 46 847.3 0 46 34 57 847.3 0 57 35 59 847.3 0 59 36 94 847.3 0 94 37 81 847.3 0 81 38 58 847.3 0 58 39 85 847.3 0 85 40 68 847.3 0 68 41 54 847.3 0 54 42 76 847.3 0 76 43 94 847.3 0 94 44 86 847.3 0 86 45 80 847.3 0 80 46 64 847.3 0 64 47 75 847.3 0 75 48 57 847.3 0 57 49 65 847.3 0 65 50 62 847.3 0 62 51 51 847.3 0 51 52 74 847.3 0 74 53 81 847.3 0 81 54 61 847.3 0 61 55 71 847.3 0 71 56 78 847.3 0 78 57 76 847.3 0 76 58 125 847.3 0 125 59 82 847.3 0 82 60 92 847.3 0 92 61 88 847.3 0 88 62 79 847.3 0 79 63 88 847.3 0 88 64 96 847.3 0 96 65 87 847.3 0 87 66 136 847.3 0 136 67 148 847.3 0 148 68 174 847.3 0 174 69 230 847.3 0 230 70 1652 847.3 0 1652 71 2451 847.3 0 2451 72 3875 847.3 0 3875 73 106 847.3 0 106 74 133 847.3 0 133 75 90 847.3 0 90 76 92 847.3 0 92 77 96 847.3 0 96 78 199 847.3 0 199 79 155 847.3 0 155 80 150 847.3 0 150 81 281 847.3 0 281 82 385 847.3 0 385 83 377 847.3 0 377 84 186 847.3 0 186 85 97 847.3 0 97 86 98 847.3 0 98 87 67 847.3 0 67 88 62 847.3 0 62 89 68 847.3 0 68 90 70 847.3 0 70 91 67 847.3 0 67 92 76 847.3 0 76 93 67 847.3 0 67 94 112 847.3 0 112 95 79 847.3 0 79 96 72 847.3 0 72 97 255 847.3 0 255 98 215 847.3 0 215 99 121 847.3 0 121 100 178 847.3 0 178 101 199 847.3 0 199 102 133 847.3 0 133 103 98 847.3 0 98 104 160 847.3 0 160 105 113 847.3 0 113 106 132 847.3 0 132 107 185 847.3 0 185 108 216 847.3 0 216 109 401 847.3 0 401 110 526 847.3 0 526 111 988 847.3 0 988 112 356 847.3 0 356 113 381 847.3 0 381 114 591 847.3 0 591 115 398 847.3 0 398 116 422 847.3 0 422 117 607 847.3 0 607 118 547 847.3 0 547 119 693 847.3 0 693 120 671 847.3 0 671 121 965 847.3 0 965 122 1279 847.3 0 1279 123 2766 847.3 0 2766 124 51007 847.3 0 51007 125 33833 847.3 0 33833 126 15379 847.3 0 15379 127 319 847.3 0 319 128 163 847.3 0 163 129 377 847.3 0 377 130 275 847.3 0 275 131 147 847.3 0 147 132 104 847.3 0 104 133 110 847.3 0 110 134 144 847.3 0 144 135 137 847.3 0 137 136 215 847.3 0 215 137 171 847.3 0 171 138 206 847.3 0 206 139 343 847.3 0 343 140 464 847.3 0 464 141 259 847.3 0 259 142 184 847.3 0 184 143 217 847.3 0 217 144 197 847.3 0 197 145 185 847.3 0 185 146 209 847.3 0 209 147 294 847.3 0 294 148 450 847.3 0 450 149 7821 847.3 0 7821 150 5086 847.3 0 5086 151 886 847.3 0 886 152 122 847.3 0 122 153 85 847.3 0 85 154 102 847.3 0 102 155 140 847.3 0 140 156 112 847.3 0 112 157 179 847.3 0 179 158 161 847.3 0 161 159 114 847.3 0 114 160 113 847.3 0 113 161 101 847.3 0 101 162 186 847.3 0 186 163 217 847.3 0 217 164 207 847.3 0 207 165 184 847.3 0 184 166 115 847.3 0 115 167 75 847.3 0 75 168 101 847.3 0 101 169 108 847.3 0 108 170 103 847.3 0 103 171 131 847.3 0 131 172 84 847.3 0 84 173 79 847.3 0 79 174 79 847.3 0 79 175 88 847.3 0 88 176 102 847.3 0 102 177 93 847.3 0 93 178 114 847.3 0 114 179 117 847.3 0 117 180 119 847.3 0 119 181 247 847.3 0 247 182 335 847.3 0 335 183 440 847.3 0 440 184 157 847.3 0 157 185 155 847.3 0 155 186 115 847.3 0 115 187 102 847.3 0 102 188 122 847.3 0 122 189 180 847.3 0 180 190 143 847.3 0 143 191 91 847.3 0 91 192 80 847.3 0 80 193 101 847.3 0 101 194 75 847.3 0 75 195 130 847.3 0 130 196 127 847.3 0 127 197 111 847.3 0 111 198 162 847.3 0 162 199 110 847.3 0 110 200 96 847.3 0 96 201 85 847.3 0 85 202 105 847.3 0 105 203 149 847.3 0 149 204 150 847.3 0 150 205 164 847.3 0 164 206 125 847.3 0 125 207 209 847.3 0 209 208 228 847.3 0 228 209 136 847.3 0 136 210 182 847.3 0 182 211 164 847.3 0 164 212 175 847.3 0 175 213 171 847.3 0 171 214 162 847.3 0 162 215 161 847.3 0 161 216 131 847.3 0 131 217 140 847.3 0 140 218 184 847.3 0 184 219 236 847.3 0 236 220 377 847.3 0 377 221 422 847.3 0 422 222 496 847.3 0 496 223 1120 847.3 0 1120 224 1361 847.3 0 1361 225 4563 847.3 0 4563 226 15619 847.3 0 15619 227 16178 847.3 0 16178 228 177 847.3 0 177 229 88 847.3 0 88 230 103 847.3 0 103 231 95 847.3 0 95 232 102 847.3 0 102 233 104 847.3 0 104 234 133 847.3 0 133 235 131 847.3 0 131 236 124 847.3 0 124 237 125 847.3 0 125 238 120 847.3 0 120 239 134 847.3 0 134 240 128 847.3 0 128 241 93 847.3 0 93 242 119 847.3 0 119 243 226 847.3 0 226 244 244 847.3 0 244 245 201 847.3 0 201 246 133 847.3 0 133 247 88 847.3 0 88 248 68 847.3 0 68 249 83 847.3 0 83 250 78 847.3 0 78 251 92 847.3 0 92 252 110 847.3 0 110 253 119 847.3 0 119 254 156 847.3 0 156 255 127 847.3 0 127 256 143 847.3 0 143 257 174 847.3 0 174 258 164 847.3 0 164 259 158 847.3 0 158 260 115 847.3 0 115 261 112 847.3 0 112 262 133 847.3 0 133 263 173 847.3 0 173 264 131 847.3 0 131 265 100 847.3 0 100 266 133 847.3 0 133 267 146 847.3 0 146 268 159 847.3 0 159 269 93 847.3 0 93 270 89 847.3 0 89 271 75 847.3 0 75 272 111 847.3 0 111 273 130 847.3 0 130 274 108 847.3 0 108 275 93 847.3 0 93 276 105 847.3 0 105 277 86 847.3 0 86 278 93 847.3 0 93 279 95 847.3 0 95 280 90 847.3 0 90 281 91 847.3 0 91 282 100 847.3 0 100 283 102 847.3 0 102 284 102 847.3 0 102 285 77 847.3 0 77 286 89 847.3 0 89 287 74 847.3 0 74 288 73 847.3 0 73 289 96 847.3 0 96 290 77 847.3 0 77 291 75 847.3 0 75 292 60 847.3 0 60 293 77 847.3 0 77 294 103 847.3 0 103 295 91 847.3 0 91 296 77 847.3 0 77 297 89 847.3 0 89 298 126 847.3 0 126 299 85 847.3 0 85 300 95 847.3 0 95 301 111 847.3 0 111 Finished in 14.95 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 242,499 Reads with adapters: 49,756 (20.5%) Reads that were too short: 206 (0.1%) Reads written (passing filters): 49,550 (20.4%) Total basepairs processed: 53,291,862 bp Total written (filtered): 13,496,029 bp (25.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49756 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 16 0.0 3 0 0 8 8 16 174 0.0 3 0 140 23 11 17 5303 0.0 3 4439 751 95 18 18 42529 0.0 3 40385 1760 311 73 19 133 0.0 3 7 83 16 27 20 96 0.0 3 3 1 43 49 21 134 0.0 3 0 0 2 132 23 7 0.0 3 0 0 0 7 25 1 0.0 3 0 0 0 1 26 7 0.0 3 0 0 0 7 28 2 0.0 3 1 0 0 1 29 9 0.0 3 1 0 0 8 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 37 1 0.0 3 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 664 0.0 3 0 0 3 661 43 1 0.0 3 0 0 0 1 45 89 0.0 3 0 0 3 86 52 4 0.0 3 0 0 0 4 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 3 0.0 3 0 0 1 2 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 66 12 0.0 3 0 0 0 12 68 325 0.0 3 0 0 1 324 69 6 0.0 3 0 0 0 6 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 3 0.0 3 0 0 0 3 88 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 93 32 0.0 3 0 0 0 32 94 2 0.0 3 0 0 0 2 95 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 35 0.0 3 0 0 0 35 105 9 0.0 3 0 0 0 9 109 2 0.0 3 0 0 1 1 110 4 0.0 3 0 0 0 4 111 2 0.0 3 0 0 0 2 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 128 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 142 8 0.0 3 0 0 0 8 143 19 0.0 3 0 0 2 17 144 19 0.0 3 0 0 2 17 145 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 7 0.0 3 0 0 0 7 156 2 0.0 3 0 0 0 2 157 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 174 6 0.0 3 0 0 0 6 179 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 248 1 0.0 3 0 1 255 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 283 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.92 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 49,550 Reads with adapters: 1,829 (3.7%) Reads written (passing filters): 49,550 (100.0%) Total basepairs processed: 13,496,029 bp Total written (filtered): 13,490,480 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1829 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.9% C: 11.8% G: 7.7% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1771 774.2 0 1771 4 55 193.6 0 55 5 2 48.4 0 2 6 1 12.1 0 1