This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.47 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 60,312 (2.5%) Reads that were too short: 12,240 (0.5%) Reads written (passing filters): 48,072 (2.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 9,138,215 bp (1.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 60312 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 212 601.0 0 212 7 2326 601.0 0 2326 8 2042 601.0 0 2042 9 173 601.0 0 173 10 67 601.0 0 67 11 82 601.0 0 82 12 73 601.0 0 73 13 65 601.0 0 65 14 85 601.0 0 85 15 79 601.0 0 79 16 55 601.0 0 55 17 87 601.0 0 87 18 72 601.0 0 72 19 60 601.0 0 60 20 101 601.0 0 101 21 103 601.0 0 103 22 61 601.0 0 61 23 71 601.0 0 71 24 82 601.0 0 82 25 112 601.0 0 112 26 107 601.0 0 107 27 343 601.0 0 343 28 188 601.0 0 188 29 167 601.0 0 167 30 65 601.0 0 65 31 70 601.0 0 70 32 63 601.0 0 63 33 88 601.0 0 88 34 74 601.0 0 74 35 108 601.0 0 108 36 93 601.0 0 93 37 114 601.0 0 114 38 73 601.0 0 73 39 71 601.0 0 71 40 66 601.0 0 66 41 73 601.0 0 73 42 61 601.0 0 61 43 58 601.0 0 58 44 80 601.0 0 80 45 80 601.0 0 80 46 89 601.0 0 89 47 80 601.0 0 80 48 79 601.0 0 79 49 69 601.0 0 69 50 89 601.0 0 89 51 98 601.0 0 98 52 92 601.0 0 92 53 85 601.0 0 85 54 79 601.0 0 79 55 68 601.0 0 68 56 78 601.0 0 78 57 99 601.0 0 99 58 241 601.0 0 241 59 432 601.0 0 432 60 206 601.0 0 206 61 71 601.0 0 71 62 91 601.0 0 91 63 67 601.0 0 67 64 65 601.0 0 65 65 73 601.0 0 73 66 74 601.0 0 74 67 60 601.0 0 60 68 96 601.0 0 96 69 109 601.0 0 109 70 163 601.0 0 163 71 244 601.0 0 244 72 1831 601.0 0 1831 73 476 601.0 0 476 74 579 601.0 0 579 75 72 601.0 0 72 76 68 601.0 0 68 77 83 601.0 0 83 78 83 601.0 0 83 79 85 601.0 0 85 80 79 601.0 0 79 81 70 601.0 0 70 82 64 601.0 0 64 83 69 601.0 0 69 84 85 601.0 0 85 85 62 601.0 0 62 86 72 601.0 0 72 87 81 601.0 0 81 88 102 601.0 0 102 89 135 601.0 0 135 90 462 601.0 0 462 91 562 601.0 0 562 92 346 601.0 0 346 93 78 601.0 0 78 94 73 601.0 0 73 95 68 601.0 0 68 96 104 601.0 0 104 97 69 601.0 0 69 98 55 601.0 0 55 99 78 601.0 0 78 100 83 601.0 0 83 101 79 601.0 0 79 102 76 601.0 0 76 103 68 601.0 0 68 104 74 601.0 0 74 105 56 601.0 0 56 106 59 601.0 0 59 107 62 601.0 0 62 108 81 601.0 0 81 109 109 601.0 0 109 110 115 601.0 0 115 111 77 601.0 0 77 112 401 601.0 0 401 113 232 601.0 0 232 114 308 601.0 0 308 115 68 601.0 0 68 116 60 601.0 0 60 117 75 601.0 0 75 118 50 601.0 0 50 119 82 601.0 0 82 120 96 601.0 0 96 121 93 601.0 0 93 122 67 601.0 0 67 123 81 601.0 0 81 124 75 601.0 0 75 125 80 601.0 0 80 126 69 601.0 0 69 127 99 601.0 0 99 128 116 601.0 0 116 129 181 601.0 0 181 130 284 601.0 0 284 131 162 601.0 0 162 132 101 601.0 0 101 133 137 601.0 0 137 134 146 601.0 0 146 135 155 601.0 0 155 136 220 601.0 0 220 137 131 601.0 0 131 138 133 601.0 0 133 139 148 601.0 0 148 140 203 601.0 0 203 141 282 601.0 0 282 142 497 601.0 0 497 143 6567 601.0 0 6567 144 6787 601.0 0 6787 145 2876 601.0 0 2876 146 110 601.0 0 110 147 89 601.0 0 89 148 126 601.0 0 126 149 137 601.0 0 137 150 102 601.0 0 102 151 70 601.0 0 70 152 117 601.0 0 117 153 92 601.0 0 92 154 117 601.0 0 117 155 1257 601.0 0 1257 156 1216 601.0 0 1216 157 1324 601.0 0 1324 158 102 601.0 0 102 159 92 601.0 0 92 160 66 601.0 0 66 161 66 601.0 0 66 162 106 601.0 0 106 163 463 601.0 0 463 164 305 601.0 0 305 165 354 601.0 0 354 166 81 601.0 0 81 167 92 601.0 0 92 168 92 601.0 0 92 169 87 601.0 0 87 170 80 601.0 0 80 171 64 601.0 0 64 172 74 601.0 0 74 173 87 601.0 0 87 174 65 601.0 0 65 175 52 601.0 0 52 176 64 601.0 0 64 177 72 601.0 0 72 178 46 601.0 0 46 179 70 601.0 0 70 180 66 601.0 0 66 181 55 601.0 0 55 182 98 601.0 0 98 183 66 601.0 0 66 184 78 601.0 0 78 185 72 601.0 0 72 186 67 601.0 0 67 187 74 601.0 0 74 188 99 601.0 0 99 189 70 601.0 0 70 190 68 601.0 0 68 191 80 601.0 0 80 192 115 601.0 0 115 193 75 601.0 0 75 194 64 601.0 0 64 195 85 601.0 0 85 196 105 601.0 0 105 197 65 601.0 0 65 198 86 601.0 0 86 199 66 601.0 0 66 200 66 601.0 0 66 201 76 601.0 0 76 202 53 601.0 0 53 203 89 601.0 0 89 204 88 601.0 0 88 205 90 601.0 0 90 206 94 601.0 0 94 207 86 601.0 0 86 208 134 601.0 0 134 209 137 601.0 0 137 210 151 601.0 0 151 211 273 601.0 0 273 212 130 601.0 0 130 213 129 601.0 0 129 214 131 601.0 0 131 215 294 601.0 0 294 216 565 601.0 0 565 217 397 601.0 0 397 218 68 601.0 0 68 219 58 601.0 0 58 220 70 601.0 0 70 221 92 601.0 0 92 222 100 601.0 0 100 223 96 601.0 0 96 224 100 601.0 0 100 225 104 601.0 0 104 226 106 601.0 0 106 227 114 601.0 0 114 228 70 601.0 0 70 229 70 601.0 0 70 230 69 601.0 0 69 231 202 601.0 0 202 232 176 601.0 0 176 233 186 601.0 0 186 234 133 601.0 0 133 235 132 601.0 0 132 236 110 601.0 0 110 237 88 601.0 0 88 238 63 601.0 0 63 239 116 601.0 0 116 240 77 601.0 0 77 241 85 601.0 0 85 242 77 601.0 0 77 243 84 601.0 0 84 244 91 601.0 0 91 245 144 601.0 0 144 246 149 601.0 0 149 247 76 601.0 0 76 248 57 601.0 0 57 249 61 601.0 0 61 250 55 601.0 0 55 251 68 601.0 0 68 252 72 601.0 0 72 253 94 601.0 0 94 254 107 601.0 0 107 255 114 601.0 0 114 256 83 601.0 0 83 257 127 601.0 0 127 258 92 601.0 0 92 259 131 601.0 0 131 260 104 601.0 0 104 261 76 601.0 0 76 262 63 601.0 0 63 263 149 601.0 0 149 264 204 601.0 0 204 265 144 601.0 0 144 266 114 601.0 0 114 267 74 601.0 0 74 268 83 601.0 0 83 269 104 601.0 0 104 270 100 601.0 0 100 271 90 601.0 0 90 272 88 601.0 0 88 273 86 601.0 0 86 274 84 601.0 0 84 275 89 601.0 0 89 276 84 601.0 0 84 277 91 601.0 0 91 278 85 601.0 0 85 279 104 601.0 0 104 280 231 601.0 0 231 281 220 601.0 0 220 282 160 601.0 0 160 283 117 601.0 0 117 284 152 601.0 0 152 285 162 601.0 0 162 286 144 601.0 0 144 287 89 601.0 0 89 288 80 601.0 0 80 289 84 601.0 0 84 290 90 601.0 0 90 291 98 601.0 0 98 292 109 601.0 0 109 293 91 601.0 0 91 294 120 601.0 0 120 295 292 601.0 0 292 296 312 601.0 0 312 297 274 601.0 0 274 298 116 601.0 0 116 299 91 601.0 0 91 300 89 601.0 0 89 301 95 601.0 0 95 Finished in 1.78 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 48,072 Reads with adapters: 2,540 (5.3%) Reads that were too short: 159 (0.3%) Reads written (passing filters): 2,381 (5.0%) Total basepairs processed: 9,138,215 bp Total written (filtered): 629,727 bp (6.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2540 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 25 0.0 3 0 20 2 3 17 2097 0.0 3 1890 170 31 6 18 27 0.0 3 2 8 2 15 19 42 0.0 3 16 6 11 9 20 2 0.0 3 0 0 0 2 23 2 0.0 3 0 0 1 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 28 21 0.0 3 0 0 0 21 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 36 4 0.0 3 0 0 0 4 45 1 0.0 3 0 0 0 1 48 29 0.0 3 0 0 0 29 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 6 0.0 3 0 0 0 6 52 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 59 9 0.0 3 0 0 0 9 60 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 63 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 4 0.0 3 0 0 0 4 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 1 1 76 2 0.0 3 0 0 0 2 79 20 0.0 3 0 0 0 20 81 3 0.0 3 0 0 0 3 82 5 0.0 3 0 0 1 4 84 1 0.0 3 0 0 0 1 85 3 0.0 3 0 0 0 3 86 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 11 0.0 3 0 0 1 10 100 4 0.0 3 0 0 0 4 101 13 0.0 3 0 0 0 13 102 2 0.0 3 0 0 0 2 104 4 0.0 3 0 0 0 4 106 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 42 0.0 3 0 0 1 41 137 3 0.0 3 0 0 0 3 138 6 0.0 3 0 0 0 6 139 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 149 5 0.0 3 0 0 0 5 150 29 0.0 3 0 0 0 29 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 9 0.0 3 0 0 0 9 165 1 0.0 3 0 0 0 1 167 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 178 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 265 2 0.0 3 0 0 0 2 271 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 2,381 Reads with adapters: 13 (0.5%) Reads written (passing filters): 2,381 (100.0%) Total basepairs processed: 629,727 bp Total written (filtered): 629,682 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 13 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.8% C: 15.4% G: 7.7% T: 23.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 37.2 0 11 6 2 0.6 0 2