This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.82 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 58,528 (2.4%) Reads that were too short: 10,710 (0.4%) Reads written (passing filters): 47,818 (1.9%) Total basepairs processed: 740,966,583 bp Total written (filtered): 9,103,467 bp (1.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 58528 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 148 601.0 0 148 7 2322 601.0 0 2322 8 2306 601.0 0 2306 9 127 601.0 0 127 10 65 601.0 0 65 11 90 601.0 0 90 12 85 601.0 0 85 13 67 601.0 0 67 14 105 601.0 0 105 15 84 601.0 0 84 16 64 601.0 0 64 17 100 601.0 0 100 18 94 601.0 0 94 19 73 601.0 0 73 20 101 601.0 0 101 21 111 601.0 0 111 22 69 601.0 0 69 23 92 601.0 0 92 24 86 601.0 0 86 25 77 601.0 0 77 26 105 601.0 0 105 27 121 601.0 0 121 28 95 601.0 0 95 29 72 601.0 0 72 30 85 601.0 0 85 31 91 601.0 0 91 32 93 601.0 0 93 33 83 601.0 0 83 34 71 601.0 0 71 35 106 601.0 0 106 36 109 601.0 0 109 37 109 601.0 0 109 38 91 601.0 0 91 39 107 601.0 0 107 40 85 601.0 0 85 41 83 601.0 0 83 42 83 601.0 0 83 43 83 601.0 0 83 44 93 601.0 0 93 45 102 601.0 0 102 46 75 601.0 0 75 47 82 601.0 0 82 48 95 601.0 0 95 49 86 601.0 0 86 50 104 601.0 0 104 51 96 601.0 0 96 52 101 601.0 0 101 53 96 601.0 0 96 54 134 601.0 0 134 55 99 601.0 0 99 56 112 601.0 0 112 57 147 601.0 0 147 58 188 601.0 0 188 59 487 601.0 0 487 60 221 601.0 0 221 61 94 601.0 0 94 62 112 601.0 0 112 63 100 601.0 0 100 64 70 601.0 0 70 65 94 601.0 0 94 66 77 601.0 0 77 67 92 601.0 0 92 68 95 601.0 0 95 69 87 601.0 0 87 70 124 601.0 0 124 71 210 601.0 0 210 72 1782 601.0 0 1782 73 533 601.0 0 533 74 587 601.0 0 587 75 83 601.0 0 83 76 66 601.0 0 66 77 90 601.0 0 90 78 104 601.0 0 104 79 80 601.0 0 80 80 72 601.0 0 72 81 106 601.0 0 106 82 78 601.0 0 78 83 99 601.0 0 99 84 81 601.0 0 81 85 79 601.0 0 79 86 95 601.0 0 95 87 98 601.0 0 98 88 108 601.0 0 108 89 146 601.0 0 146 90 320 601.0 0 320 91 315 601.0 0 315 92 229 601.0 0 229 93 142 601.0 0 142 94 85 601.0 0 85 95 88 601.0 0 88 96 112 601.0 0 112 97 89 601.0 0 89 98 69 601.0 0 69 99 110 601.0 0 110 100 99 601.0 0 99 101 76 601.0 0 76 102 120 601.0 0 120 103 83 601.0 0 83 104 105 601.0 0 105 105 97 601.0 0 97 106 90 601.0 0 90 107 103 601.0 0 103 108 105 601.0 0 105 109 111 601.0 0 111 110 107 601.0 0 107 111 87 601.0 0 87 112 154 601.0 0 154 113 128 601.0 0 128 114 153 601.0 0 153 115 68 601.0 0 68 116 88 601.0 0 88 117 67 601.0 0 67 118 57 601.0 0 57 119 111 601.0 0 111 120 116 601.0 0 116 121 100 601.0 0 100 122 81 601.0 0 81 123 105 601.0 0 105 124 114 601.0 0 114 125 92 601.0 0 92 126 80 601.0 0 80 127 86 601.0 0 86 128 119 601.0 0 119 129 158 601.0 0 158 130 204 601.0 0 204 131 171 601.0 0 171 132 110 601.0 0 110 133 152 601.0 0 152 134 174 601.0 0 174 135 131 601.0 0 131 136 227 601.0 0 227 137 126 601.0 0 126 138 153 601.0 0 153 139 132 601.0 0 132 140 186 601.0 0 186 141 228 601.0 0 228 142 384 601.0 0 384 143 6133 601.0 0 6133 144 6157 601.0 0 6157 145 2686 601.0 0 2686 146 97 601.0 0 97 147 111 601.0 0 111 148 123 601.0 0 123 149 152 601.0 0 152 150 107 601.0 0 107 151 112 601.0 0 112 152 132 601.0 0 132 153 111 601.0 0 111 154 110 601.0 0 110 155 1540 601.0 0 1540 156 1424 601.0 0 1424 157 1597 601.0 0 1597 158 124 601.0 0 124 159 97 601.0 0 97 160 81 601.0 0 81 161 99 601.0 0 99 162 127 601.0 0 127 163 386 601.0 0 386 164 234 601.0 0 234 165 317 601.0 0 317 166 104 601.0 0 104 167 91 601.0 0 91 168 79 601.0 0 79 169 83 601.0 0 83 170 112 601.0 0 112 171 92 601.0 0 92 172 62 601.0 0 62 173 81 601.0 0 81 174 83 601.0 0 83 175 92 601.0 0 92 176 93 601.0 0 93 177 79 601.0 0 79 178 75 601.0 0 75 179 84 601.0 0 84 180 103 601.0 0 103 181 71 601.0 0 71 182 84 601.0 0 84 183 77 601.0 0 77 184 91 601.0 0 91 185 73 601.0 0 73 186 67 601.0 0 67 187 72 601.0 0 72 188 83 601.0 0 83 189 91 601.0 0 91 190 82 601.0 0 82 191 89 601.0 0 89 192 83 601.0 0 83 193 97 601.0 0 97 194 70 601.0 0 70 195 91 601.0 0 91 196 107 601.0 0 107 197 105 601.0 0 105 198 77 601.0 0 77 199 73 601.0 0 73 200 91 601.0 0 91 201 79 601.0 0 79 202 81 601.0 0 81 203 76 601.0 0 76 204 86 601.0 0 86 205 78 601.0 0 78 206 117 601.0 0 117 207 107 601.0 0 107 208 114 601.0 0 114 209 159 601.0 0 159 210 154 601.0 0 154 211 91 601.0 0 91 212 90 601.0 0 90 213 110 601.0 0 110 214 104 601.0 0 104 215 228 601.0 0 228 216 527 601.0 0 527 217 333 601.0 0 333 218 79 601.0 0 79 219 72 601.0 0 72 220 103 601.0 0 103 221 89 601.0 0 89 222 93 601.0 0 93 223 83 601.0 0 83 224 103 601.0 0 103 225 98 601.0 0 98 226 98 601.0 0 98 227 111 601.0 0 111 228 93 601.0 0 93 229 70 601.0 0 70 230 86 601.0 0 86 231 102 601.0 0 102 232 90 601.0 0 90 233 104 601.0 0 104 234 108 601.0 0 108 235 70 601.0 0 70 236 100 601.0 0 100 237 103 601.0 0 103 238 77 601.0 0 77 239 85 601.0 0 85 240 80 601.0 0 80 241 88 601.0 0 88 242 83 601.0 0 83 243 64 601.0 0 64 244 91 601.0 0 91 245 123 601.0 0 123 246 98 601.0 0 98 247 83 601.0 0 83 248 83 601.0 0 83 249 96 601.0 0 96 250 67 601.0 0 67 251 78 601.0 0 78 252 88 601.0 0 88 253 96 601.0 0 96 254 96 601.0 0 96 255 110 601.0 0 110 256 67 601.0 0 67 257 121 601.0 0 121 258 96 601.0 0 96 259 115 601.0 0 115 260 112 601.0 0 112 261 103 601.0 0 103 262 84 601.0 0 84 263 105 601.0 0 105 264 114 601.0 0 114 265 95 601.0 0 95 266 78 601.0 0 78 267 106 601.0 0 106 268 88 601.0 0 88 269 104 601.0 0 104 270 84 601.0 0 84 271 100 601.0 0 100 272 101 601.0 0 101 273 102 601.0 0 102 274 66 601.0 0 66 275 81 601.0 0 81 276 88 601.0 0 88 277 92 601.0 0 92 278 114 601.0 0 114 279 97 601.0 0 97 280 161 601.0 0 161 281 173 601.0 0 173 282 135 601.0 0 135 283 102 601.0 0 102 284 114 601.0 0 114 285 109 601.0 0 109 286 100 601.0 0 100 287 89 601.0 0 89 288 91 601.0 0 91 289 87 601.0 0 87 290 113 601.0 0 113 291 110 601.0 0 110 292 96 601.0 0 96 293 105 601.0 0 105 294 80 601.0 0 80 295 149 601.0 0 149 296 168 601.0 0 168 297 160 601.0 0 160 298 86 601.0 0 86 299 100 601.0 0 100 300 92 601.0 0 92 301 81 601.0 0 81 Finished in 1.99 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 47,818 Reads with adapters: 2,384 (5.0%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 2,381 (5.0%) Total basepairs processed: 9,103,467 bp Total written (filtered): 649,820 bp (7.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2384 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 27 0.0 3 0 27 20 2334 0.0 4 2301 31 2 21 13 0.0 4 5 7 1 22 4 0.0 4 1 1 2 39 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 1 108 1 0.0 4 0 0 1 156 1 0.0 4 0 0 0 0 1 201 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 2,381 Reads with adapters: 221 (9.3%) Reads written (passing filters): 2,381 (100.0%) Total basepairs processed: 649,820 bp Total written (filtered): 647,434 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 221 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 5.4% G: 91.0% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 37.2 0 1 9 3 0.0 0 0 3 10 31 0.0 1 2 29 11 186 0.0 1 3 183 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.