This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.74 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 366,248 (14.4%) Reads that were too short: 9,808 (0.4%) Reads written (passing filters): 356,440 (14.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 68,952,812 bp (9.0%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 366248 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 61 621.6 0 61 7 2068 621.6 0 2068 8 2380 621.6 0 2380 9 36 621.6 0 36 10 41 621.6 0 41 11 46 621.6 0 46 12 83 621.6 0 83 13 97 621.6 0 97 14 99 621.6 0 99 15 59 621.6 0 59 16 30 621.6 0 30 17 48 621.6 0 48 18 52 621.6 0 52 19 77 621.6 0 77 20 157 621.6 0 157 21 127 621.6 0 127 22 79 621.6 0 79 23 72 621.6 0 72 24 37 621.6 0 37 25 72 621.6 0 72 26 71 621.6 0 71 27 57 621.6 0 57 28 42 621.6 0 42 29 90 621.6 0 90 30 66 621.6 0 66 31 89 621.6 0 89 32 69 621.6 0 69 33 87 621.6 0 87 34 76 621.6 0 76 35 81 621.6 0 81 36 119 621.6 0 119 37 218 621.6 0 218 38 73 621.6 0 73 39 52 621.6 0 52 40 65 621.6 0 65 41 68 621.6 0 68 42 44 621.6 0 44 43 91 621.6 0 91 44 67 621.6 0 67 45 33 621.6 0 33 46 61 621.6 0 61 47 67 621.6 0 67 48 68 621.6 0 68 49 55 621.6 0 55 50 55 621.6 0 55 51 69 621.6 0 69 52 56 621.6 0 56 53 79 621.6 0 79 54 74 621.6 0 74 55 59 621.6 0 59 56 51 621.6 0 51 57 71 621.6 0 71 58 99 621.6 0 99 59 37 621.6 0 37 60 67 621.6 0 67 61 86 621.6 0 86 62 111 621.6 0 111 63 71 621.6 0 71 64 79 621.6 0 79 65 223 621.6 0 223 66 163 621.6 0 163 67 100 621.6 0 100 68 67 621.6 0 67 69 77 621.6 0 77 70 73 621.6 0 73 71 65 621.6 0 65 72 112 621.6 0 112 73 71 621.6 0 71 74 89 621.6 0 89 75 90 621.6 0 90 76 79 621.6 0 79 77 138 621.6 0 138 78 358 621.6 0 358 79 172 621.6 0 172 80 687 621.6 0 687 81 247 621.6 0 247 82 157 621.6 0 157 83 78 621.6 0 78 84 151 621.6 0 151 85 104 621.6 0 104 86 224 621.6 0 224 87 236 621.6 0 236 88 389 621.6 0 389 89 565 621.6 0 565 90 1347 621.6 0 1347 91 2257 621.6 0 2257 92 403 621.6 0 403 93 604 621.6 0 604 94 1125 621.6 0 1125 95 512 621.6 0 512 96 412 621.6 0 412 97 924 621.6 0 924 98 870 621.6 0 870 99 1097 621.6 0 1097 100 1137 621.6 0 1137 101 1579 621.6 0 1579 102 2892 621.6 0 2892 103 4896 621.6 0 4896 104 136010 621.6 0 136010 105 72395 621.6 0 72395 106 78253 621.6 0 78253 107 278 621.6 0 278 108 35 621.6 0 35 109 41 621.6 0 41 110 45 621.6 0 45 111 74 621.6 0 74 112 60 621.6 0 60 113 52 621.6 0 52 114 58 621.6 0 58 115 71 621.6 0 71 116 47 621.6 0 47 117 84 621.6 0 84 118 46 621.6 0 46 119 45 621.6 0 45 120 51 621.6 0 51 121 76 621.6 0 76 122 58 621.6 0 58 123 74 621.6 0 74 124 328 621.6 0 328 125 203 621.6 0 203 126 131 621.6 0 131 127 121 621.6 0 121 128 202 621.6 0 202 129 58 621.6 0 58 130 55 621.6 0 55 131 49 621.6 0 49 132 139 621.6 0 139 133 54 621.6 0 54 134 47 621.6 0 47 135 38 621.6 0 38 136 44 621.6 0 44 137 46 621.6 0 46 138 58 621.6 0 58 139 74 621.6 0 74 140 77 621.6 0 77 141 71 621.6 0 71 142 110 621.6 0 110 143 60 621.6 0 60 144 80 621.6 0 80 145 45 621.6 0 45 146 89 621.6 0 89 147 140 621.6 0 140 148 75 621.6 0 75 149 67 621.6 0 67 150 75 621.6 0 75 151 133 621.6 0 133 152 117 621.6 0 117 153 227 621.6 0 227 154 161 621.6 0 161 155 214 621.6 0 214 156 258 621.6 0 258 157 527 621.6 0 527 158 1136 621.6 0 1136 159 13336 621.6 0 13336 160 4793 621.6 0 4793 161 8268 621.6 0 8268 162 75 621.6 0 75 163 56 621.6 0 56 164 51 621.6 0 51 165 59 621.6 0 59 166 77 621.6 0 77 167 392 621.6 0 392 168 157 621.6 0 157 169 134 621.6 0 134 170 45 621.6 0 45 171 86 621.6 0 86 172 111 621.6 0 111 173 122 621.6 0 122 174 69 621.6 0 69 175 42 621.6 0 42 176 47 621.6 0 47 177 64 621.6 0 64 178 65 621.6 0 65 179 51 621.6 0 51 180 62 621.6 0 62 181 75 621.6 0 75 182 66 621.6 0 66 183 80 621.6 0 80 184 142 621.6 0 142 185 77 621.6 0 77 186 137 621.6 0 137 187 56 621.6 0 56 188 71 621.6 0 71 189 94 621.6 0 94 190 46 621.6 0 46 191 42 621.6 0 42 192 58 621.6 0 58 193 51 621.6 0 51 194 42 621.6 0 42 195 40 621.6 0 40 196 43 621.6 0 43 197 67 621.6 0 67 198 99 621.6 0 99 199 90 621.6 0 90 200 250 621.6 0 250 201 126 621.6 0 126 202 225 621.6 0 225 203 53 621.6 0 53 204 61 621.6 0 61 205 69 621.6 0 69 206 56 621.6 0 56 207 82 621.6 0 82 208 72 621.6 0 72 209 71 621.6 0 71 210 43 621.6 0 43 211 51 621.6 0 51 212 48 621.6 0 48 213 63 621.6 0 63 214 48 621.6 0 48 215 72 621.6 0 72 216 53 621.6 0 53 217 48 621.6 0 48 218 76 621.6 0 76 219 83 621.6 0 83 220 112 621.6 0 112 221 61 621.6 0 61 222 52 621.6 0 52 223 63 621.6 0 63 224 58 621.6 0 58 225 52 621.6 0 52 226 64 621.6 0 64 227 92 621.6 0 92 228 99 621.6 0 99 229 82 621.6 0 82 230 71 621.6 0 71 231 54 621.6 0 54 232 67 621.6 0 67 233 62 621.6 0 62 234 57 621.6 0 57 235 57 621.6 0 57 236 75 621.6 0 75 237 55 621.6 0 55 238 110 621.6 0 110 239 112 621.6 0 112 240 481 621.6 0 481 241 555 621.6 0 555 242 732 621.6 0 732 243 180 621.6 0 180 244 64 621.6 0 64 245 57 621.6 0 57 246 64 621.6 0 64 247 69 621.6 0 69 248 86 621.6 0 86 249 67 621.6 0 67 250 58 621.6 0 58 251 60 621.6 0 60 252 68 621.6 0 68 253 67 621.6 0 67 254 89 621.6 0 89 255 81 621.6 0 81 256 95 621.6 0 95 257 77 621.6 0 77 258 62 621.6 0 62 259 61 621.6 0 61 260 81 621.6 0 81 261 72 621.6 0 72 262 67 621.6 0 67 263 47 621.6 0 47 264 62 621.6 0 62 265 56 621.6 0 56 266 60 621.6 0 60 267 83 621.6 0 83 268 92 621.6 0 92 269 90 621.6 0 90 270 87 621.6 0 87 271 91 621.6 0 91 272 75 621.6 0 75 273 93 621.6 0 93 274 136 621.6 0 136 275 114 621.6 0 114 276 113 621.6 0 113 277 104 621.6 0 104 278 106 621.6 0 106 279 147 621.6 0 147 280 196 621.6 0 196 281 179 621.6 0 179 282 127 621.6 0 127 283 192 621.6 0 192 284 52 621.6 0 52 285 51 621.6 0 51 286 57 621.6 0 57 287 81 621.6 0 81 288 68 621.6 0 68 289 79 621.6 0 79 290 78 621.6 0 78 291 61 621.6 0 61 292 77 621.6 0 77 293 93 621.6 0 93 294 90 621.6 0 90 295 89 621.6 0 89 296 76 621.6 0 76 297 52 621.6 0 52 298 78 621.6 0 78 299 109 621.6 0 109 300 143 621.6 0 143 301 202 621.6 0 202 Finished in 13.99 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 356,440 Reads with adapters: 9,604 (2.7%) Reads that were too short: 51 (0.0%) Reads written (passing filters): 9,553 (2.7%) Total basepairs processed: 68,952,812 bp Total written (filtered): 1,847,090 bp (2.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9604 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 1 19 24 0.0 3 0 24 20 2342 0.0 4 2299 41 2 21 4 0.0 4 0 4 22 2 0.0 4 0 0 2 23 2 0.0 4 0 0 0 2 27 2 0.0 4 0 0 0 0 2 29 4 0.0 4 0 0 0 0 4 30 6671 0.0 4 0 0 0 0 6671 32 3 0.0 4 0 0 0 0 3 33 6 0.0 4 0 0 0 0 6 41 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 56 1 0.0 4 1 71 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 2 0.0 4 1 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 0 6 127 475 0.0 4 0 0 0 0 475 128 4 0.0 4 0 0 0 0 4 136 2 0.0 4 0 0 0 0 2 137 2 0.0 4 0 0 0 0 2 138 2 0.0 4 0 0 0 0 2 139 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 160 2 0.0 4 0 0 0 1 1 170 1 0.0 4 0 0 0 0 1 173 1 0.0 4 1 185 22 0.0 4 0 0 0 0 22 186 7 0.0 4 0 0 0 0 7 187 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (28 us/read; 2.12 M reads/minute). === Summary === Total reads processed: 9,553 Reads with adapters: 376 (3.9%) Reads written (passing filters): 9,553 (100.0%) Total basepairs processed: 1,847,090 bp Total written (filtered): 1,843,778 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 376 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 26.9% C: 3.7% G: 66.2% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 67 149.3 0 67 4 36 37.3 0 36 5 6 9.3 0 6 9 4 0.0 0 2 2 11 262 0.0 1 2 260 19 1 0.0 1 0 1