This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.91 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 318,412 (12.5%) Reads that were too short: 146,638 (5.8%) Reads written (passing filters): 171,774 (6.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 32,085,964 bp (4.2%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 318412 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 302 621.6 0 302 7 7874 621.6 0 7874 8 6153 621.6 0 6153 9 71 621.6 0 71 10 65 621.6 0 65 11 233 621.6 0 233 12 80 621.6 0 80 13 64 621.6 0 64 14 63 621.6 0 63 15 64 621.6 0 64 16 68 621.6 0 68 17 57 621.6 0 57 18 69 621.6 0 69 19 61 621.6 0 61 20 66 621.6 0 66 21 71 621.6 0 71 22 71 621.6 0 71 23 66 621.6 0 66 24 72 621.6 0 72 25 63 621.6 0 63 26 73 621.6 0 73 27 93 621.6 0 93 28 96 621.6 0 96 29 86 621.6 0 86 30 137 621.6 0 137 31 114 621.6 0 114 32 57 621.6 0 57 33 70 621.6 0 70 34 55 621.6 0 55 35 72 621.6 0 72 36 109 621.6 0 109 37 92 621.6 0 92 38 87 621.6 0 87 39 121 621.6 0 121 40 93 621.6 0 93 41 61 621.6 0 61 42 100 621.6 0 100 43 121 621.6 0 121 44 88 621.6 0 88 45 104 621.6 0 104 46 91 621.6 0 91 47 106 621.6 0 106 48 96 621.6 0 96 49 77 621.6 0 77 50 82 621.6 0 82 51 77 621.6 0 77 52 79 621.6 0 79 53 151 621.6 0 151 54 87 621.6 0 87 55 83 621.6 0 83 56 98 621.6 0 98 57 76 621.6 0 76 58 136 621.6 0 136 59 147 621.6 0 147 60 129 621.6 0 129 61 115 621.6 0 115 62 128 621.6 0 128 63 131 621.6 0 131 64 128 621.6 0 128 65 132 621.6 0 132 66 201 621.6 0 201 67 170 621.6 0 170 68 268 621.6 0 268 69 778 621.6 0 778 70 4937 621.6 0 4937 71 5097 621.6 0 5097 72 3472 621.6 0 3472 73 116 621.6 0 116 74 114 621.6 0 114 75 66 621.6 0 66 76 112 621.6 0 112 77 81 621.6 0 81 78 135 621.6 0 135 79 121 621.6 0 121 80 132 621.6 0 132 81 188 621.6 0 188 82 223 621.6 0 223 83 281 621.6 0 281 84 121 621.6 0 121 85 101 621.6 0 101 86 106 621.6 0 106 87 72 621.6 0 72 88 76 621.6 0 76 89 65 621.6 0 65 90 57 621.6 0 57 91 78 621.6 0 78 92 74 621.6 0 74 93 81 621.6 0 81 94 88 621.6 0 88 95 80 621.6 0 80 96 83 621.6 0 83 97 107 621.6 0 107 98 143 621.6 0 143 99 172 621.6 0 172 100 240 621.6 0 240 101 186 621.6 0 186 102 119 621.6 0 119 103 142 621.6 0 142 104 108 621.6 0 108 105 107 621.6 0 107 106 106 621.6 0 106 107 125 621.6 0 125 108 162 621.6 0 162 109 203 621.6 0 203 110 378 621.6 0 378 111 715 621.6 0 715 112 222 621.6 0 222 113 284 621.6 0 284 114 483 621.6 0 483 115 240 621.6 0 240 116 242 621.6 0 242 117 390 621.6 0 390 118 364 621.6 0 364 119 564 621.6 0 564 120 424 621.6 0 424 121 586 621.6 0 586 122 889 621.6 0 889 123 1554 621.6 0 1554 124 27934 621.6 0 27934 125 17331 621.6 0 17331 126 32000 621.6 0 32000 127 688 621.6 0 688 128 242 621.6 0 242 129 431 621.6 0 431 130 252 621.6 0 252 131 306 621.6 0 306 132 115 621.6 0 115 133 118 621.6 0 118 134 134 621.6 0 134 135 205 621.6 0 205 136 393 621.6 0 393 137 254 621.6 0 254 138 226 621.6 0 226 139 252 621.6 0 252 140 204 621.6 0 204 141 158 621.6 0 158 142 289 621.6 0 289 143 189 621.6 0 189 144 288 621.6 0 288 145 361 621.6 0 361 146 347 621.6 0 347 147 526 621.6 0 526 148 988 621.6 0 988 149 16919 621.6 0 16919 150 8126 621.6 0 8126 151 9777 621.6 0 9777 152 97 621.6 0 97 153 61 621.6 0 61 154 105 621.6 0 105 155 89 621.6 0 89 156 109 621.6 0 109 157 120 621.6 0 120 158 124 621.6 0 124 159 94 621.6 0 94 160 124 621.6 0 124 161 84 621.6 0 84 162 152 621.6 0 152 163 206 621.6 0 206 164 192 621.6 0 192 165 122 621.6 0 122 166 92 621.6 0 92 167 80 621.6 0 80 168 83 621.6 0 83 169 101 621.6 0 101 170 76 621.6 0 76 171 79 621.6 0 79 172 84 621.6 0 84 173 76 621.6 0 76 174 76 621.6 0 76 175 73 621.6 0 73 176 74 621.6 0 74 177 85 621.6 0 85 178 96 621.6 0 96 179 102 621.6 0 102 180 90 621.6 0 90 181 232 621.6 0 232 182 185 621.6 0 185 183 365 621.6 0 365 184 96 621.6 0 96 185 111 621.6 0 111 186 132 621.6 0 132 187 96 621.6 0 96 188 192 621.6 0 192 189 106 621.6 0 106 190 121 621.6 0 121 191 93 621.6 0 93 192 96 621.6 0 96 193 84 621.6 0 84 194 122 621.6 0 122 195 230 621.6 0 230 196 124 621.6 0 124 197 133 621.6 0 133 198 574 621.6 0 574 199 90 621.6 0 90 200 89 621.6 0 89 201 64 621.6 0 64 202 81 621.6 0 81 203 164 621.6 0 164 204 194 621.6 0 194 205 242 621.6 0 242 206 232 621.6 0 232 207 361 621.6 0 361 208 828 621.6 0 828 209 197 621.6 0 197 210 264 621.6 0 264 211 204 621.6 0 204 212 258 621.6 0 258 213 699 621.6 0 699 214 448 621.6 0 448 215 375 621.6 0 375 216 164 621.6 0 164 217 159 621.6 0 159 218 332 621.6 0 332 219 376 621.6 0 376 220 618 621.6 0 618 221 905 621.6 0 905 222 892 621.6 0 892 223 3217 621.6 0 3217 224 6290 621.6 0 6290 225 37203 621.6 0 37203 226 51165 621.6 0 51165 227 33382 621.6 0 33382 228 230 621.6 0 230 229 91 621.6 0 91 230 118 621.6 0 118 231 173 621.6 0 173 232 111 621.6 0 111 233 84 621.6 0 84 234 123 621.6 0 123 235 134 621.6 0 134 236 131 621.6 0 131 237 108 621.6 0 108 238 94 621.6 0 94 239 105 621.6 0 105 240 116 621.6 0 116 241 98 621.6 0 98 242 114 621.6 0 114 243 165 621.6 0 165 244 175 621.6 0 175 245 175 621.6 0 175 246 114 621.6 0 114 247 61 621.6 0 61 248 65 621.6 0 65 249 65 621.6 0 65 250 76 621.6 0 76 251 67 621.6 0 67 252 90 621.6 0 90 253 82 621.6 0 82 254 127 621.6 0 127 255 125 621.6 0 125 256 145 621.6 0 145 257 134 621.6 0 134 258 105 621.6 0 105 259 102 621.6 0 102 260 103 621.6 0 103 261 75 621.6 0 75 262 176 621.6 0 176 263 194 621.6 0 194 264 176 621.6 0 176 265 166 621.6 0 166 266 157 621.6 0 157 267 138 621.6 0 138 268 165 621.6 0 165 269 69 621.6 0 69 270 64 621.6 0 64 271 71 621.6 0 71 272 80 621.6 0 80 273 104 621.6 0 104 274 88 621.6 0 88 275 68 621.6 0 68 276 69 621.6 0 69 277 82 621.6 0 82 278 73 621.6 0 73 279 63 621.6 0 63 280 93 621.6 0 93 281 97 621.6 0 97 282 67 621.6 0 67 283 68 621.6 0 68 284 70 621.6 0 70 285 55 621.6 0 55 286 72 621.6 0 72 287 79 621.6 0 79 288 60 621.6 0 60 289 74 621.6 0 74 290 59 621.6 0 59 291 65 621.6 0 65 292 65 621.6 0 65 293 67 621.6 0 67 294 61 621.6 0 61 295 78 621.6 0 78 296 55 621.6 0 55 297 63 621.6 0 63 298 61 621.6 0 61 299 64 621.6 0 64 300 58 621.6 0 58 301 73 621.6 0 73 Finished in 6.21 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 171,774 Reads with adapters: 9,783 (5.7%) Reads that were too short: 402 (0.2%) Reads written (passing filters): 9,381 (5.5%) Total basepairs processed: 32,085,964 bp Total written (filtered): 2,337,445 bp (7.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9783 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 3 3 16 44 0.0 3 0 34 9 1 17 1117 0.0 3 929 150 27 11 18 6509 0.0 3 5922 451 106 30 19 42 0.0 3 11 18 6 7 20 64 0.0 3 15 4 23 22 21 6 0.0 3 0 0 0 6 23 1 0.0 3 1 25 2 0.0 3 0 0 0 2 26 5 0.0 3 0 0 0 5 29 7 0.0 3 0 0 0 7 32 4 0.0 3 0 0 0 4 33 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 0 2 42 1151 0.0 3 0 0 6 1145 44 2 0.0 3 0 0 0 2 45 145 0.0 3 0 0 4 141 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 52 6 0.0 3 0 0 0 6 53 1 0.0 3 0 0 1 55 4 0.0 3 0 0 1 3 58 3 0.0 3 0 0 0 3 62 4 0.0 3 0 0 0 4 64 2 0.0 3 0 0 0 2 66 41 0.0 3 0 0 1 40 67 3 0.0 3 0 0 0 3 68 206 0.0 3 0 0 2 204 69 6 0.0 3 0 0 0 6 70 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 0 4 85 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 55 0.0 3 0 0 1 54 94 2 0.0 3 0 0 0 2 95 5 0.0 3 0 0 0 5 96 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 102 54 0.0 3 0 0 1 53 103 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 7 0.0 3 0 0 0 7 113 5 0.0 3 0 0 0 5 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 130 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 141 4 0.0 3 0 0 0 4 142 15 0.0 3 0 0 0 15 143 58 0.0 3 0 0 1 57 144 93 0.0 3 0 0 7 86 145 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 154 7 0.0 3 0 0 0 7 155 4 0.0 3 0 0 0 4 156 3 0.0 3 0 0 0 3 157 5 0.0 3 0 0 0 5 158 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 3 0.0 3 0 0 0 3 172 1 0.0 3 0 0 0 1 174 9 0.0 3 0 0 0 9 176 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 1 218 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 9,381 Reads with adapters: 11 (0.1%) Reads written (passing filters): 9,381 (100.0%) Total basepairs processed: 2,337,445 bp Total written (filtered): 2,337,404 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 11 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.1% C: 9.1% G: 72.7% T: 9.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 146.6 0 4 4 6 36.6 0 6 5 1 9.2 0 1