This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.55 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 349,621 (13.7%) Reads that were too short: 180,650 (7.1%) Reads written (passing filters): 168,971 (6.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 31,446,269 bp (4.1%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 349621 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 207 621.6 0 207 7 7496 621.6 0 7496 8 7301 621.6 0 7301 9 44 621.6 0 44 10 43 621.6 0 43 11 66 621.6 0 66 12 49 621.6 0 49 13 45 621.6 0 45 14 46 621.6 0 46 15 43 621.6 0 43 16 41 621.6 0 41 17 55 621.6 0 55 18 38 621.6 0 38 19 35 621.6 0 35 20 69 621.6 0 69 21 51 621.6 0 51 22 52 621.6 0 52 23 47 621.6 0 47 24 50 621.6 0 50 25 37 621.6 0 37 26 44 621.6 0 44 27 53 621.6 0 53 28 64 621.6 0 64 29 35 621.6 0 35 30 82 621.6 0 82 31 47 621.6 0 47 32 40 621.6 0 40 33 47 621.6 0 47 34 54 621.6 0 54 35 53 621.6 0 53 36 89 621.6 0 89 37 62 621.6 0 62 38 63 621.6 0 63 39 103 621.6 0 103 40 79 621.6 0 79 41 39 621.6 0 39 42 79 621.6 0 79 43 106 621.6 0 106 44 71 621.6 0 71 45 63 621.6 0 63 46 65 621.6 0 65 47 48 621.6 0 48 48 74 621.6 0 74 49 71 621.6 0 71 50 70 621.6 0 70 51 64 621.6 0 64 52 46 621.6 0 46 53 99 621.6 0 99 54 51 621.6 0 51 55 64 621.6 0 64 56 69 621.6 0 69 57 62 621.6 0 62 58 116 621.6 0 116 59 94 621.6 0 94 60 90 621.6 0 90 61 87 621.6 0 87 62 93 621.6 0 93 63 94 621.6 0 94 64 81 621.6 0 81 65 93 621.6 0 93 66 161 621.6 0 161 67 121 621.6 0 121 68 190 621.6 0 190 69 568 621.6 0 568 70 4843 621.6 0 4843 71 4674 621.6 0 4674 72 3482 621.6 0 3482 73 76 621.6 0 76 74 86 621.6 0 86 75 38 621.6 0 38 76 56 621.6 0 56 77 51 621.6 0 51 78 48 621.6 0 48 79 64 621.6 0 64 80 69 621.6 0 69 81 143 621.6 0 143 82 124 621.6 0 124 83 234 621.6 0 234 84 92 621.6 0 92 85 73 621.6 0 73 86 51 621.6 0 51 87 52 621.6 0 52 88 53 621.6 0 53 89 62 621.6 0 62 90 72 621.6 0 72 91 80 621.6 0 80 92 50 621.6 0 50 93 61 621.6 0 61 94 75 621.6 0 75 95 76 621.6 0 76 96 65 621.6 0 65 97 81 621.6 0 81 98 128 621.6 0 128 99 93 621.6 0 93 100 176 621.6 0 176 101 123 621.6 0 123 102 105 621.6 0 105 103 94 621.6 0 94 104 83 621.6 0 83 105 93 621.6 0 93 106 106 621.6 0 106 107 116 621.6 0 116 108 128 621.6 0 128 109 177 621.6 0 177 110 356 621.6 0 356 111 671 621.6 0 671 112 184 621.6 0 184 113 235 621.6 0 235 114 464 621.6 0 464 115 195 621.6 0 195 116 206 621.6 0 206 117 403 621.6 0 403 118 377 621.6 0 377 119 474 621.6 0 474 120 345 621.6 0 345 121 553 621.6 0 553 122 742 621.6 0 742 123 1229 621.6 0 1229 124 28082 621.6 0 28082 125 17405 621.6 0 17405 126 33422 621.6 0 33422 127 670 621.6 0 670 128 180 621.6 0 180 129 215 621.6 0 215 130 103 621.6 0 103 131 184 621.6 0 184 132 77 621.6 0 77 133 72 621.6 0 72 134 89 621.6 0 89 135 202 621.6 0 202 136 319 621.6 0 319 137 170 621.6 0 170 138 183 621.6 0 183 139 202 621.6 0 202 140 168 621.6 0 168 141 112 621.6 0 112 142 268 621.6 0 268 143 177 621.6 0 177 144 216 621.6 0 216 145 319 621.6 0 319 146 225 621.6 0 225 147 389 621.6 0 389 148 639 621.6 0 639 149 18122 621.6 0 18122 150 8943 621.6 0 8943 151 10521 621.6 0 10521 152 81 621.6 0 81 153 50 621.6 0 50 154 71 621.6 0 71 155 74 621.6 0 74 156 66 621.6 0 66 157 62 621.6 0 62 158 94 621.6 0 94 159 57 621.6 0 57 160 59 621.6 0 59 161 57 621.6 0 57 162 85 621.6 0 85 163 212 621.6 0 212 164 71 621.6 0 71 165 116 621.6 0 116 166 67 621.6 0 67 167 58 621.6 0 58 168 47 621.6 0 47 169 62 621.6 0 62 170 54 621.6 0 54 171 55 621.6 0 55 172 53 621.6 0 53 173 45 621.6 0 45 174 42 621.6 0 42 175 54 621.6 0 54 176 56 621.6 0 56 177 51 621.6 0 51 178 53 621.6 0 53 179 58 621.6 0 58 180 56 621.6 0 56 181 149 621.6 0 149 182 107 621.6 0 107 183 291 621.6 0 291 184 65 621.6 0 65 185 65 621.6 0 65 186 71 621.6 0 71 187 57 621.6 0 57 188 161 621.6 0 161 189 89 621.6 0 89 190 93 621.6 0 93 191 96 621.6 0 96 192 79 621.6 0 79 193 69 621.6 0 69 194 129 621.6 0 129 195 271 621.6 0 271 196 111 621.6 0 111 197 92 621.6 0 92 198 710 621.6 0 710 199 79 621.6 0 79 200 51 621.6 0 51 201 50 621.6 0 50 202 62 621.6 0 62 203 85 621.6 0 85 204 84 621.6 0 84 205 139 621.6 0 139 206 140 621.6 0 140 207 352 621.6 0 352 208 935 621.6 0 935 209 156 621.6 0 156 210 294 621.6 0 294 211 180 621.6 0 180 212 225 621.6 0 225 213 931 621.6 0 931 214 547 621.6 0 547 215 450 621.6 0 450 216 116 621.6 0 116 217 168 621.6 0 168 218 281 621.6 0 281 219 330 621.6 0 330 220 577 621.6 0 577 221 856 621.6 0 856 222 892 621.6 0 892 223 3523 621.6 0 3523 224 6189 621.6 0 6189 225 49053 621.6 0 49053 226 64564 621.6 0 64564 227 44635 621.6 0 44635 228 231 621.6 0 231 229 87 621.6 0 87 230 76 621.6 0 76 231 93 621.6 0 93 232 81 621.6 0 81 233 65 621.6 0 65 234 59 621.6 0 59 235 73 621.6 0 73 236 74 621.6 0 74 237 69 621.6 0 69 238 69 621.6 0 69 239 56 621.6 0 56 240 66 621.6 0 66 241 52 621.6 0 52 242 83 621.6 0 83 243 101 621.6 0 101 244 110 621.6 0 110 245 105 621.6 0 105 246 68 621.6 0 68 247 56 621.6 0 56 248 45 621.6 0 45 249 49 621.6 0 49 250 45 621.6 0 45 251 51 621.6 0 51 252 46 621.6 0 46 253 68 621.6 0 68 254 78 621.6 0 78 255 72 621.6 0 72 256 81 621.6 0 81 257 95 621.6 0 95 258 82 621.6 0 82 259 60 621.6 0 60 260 53 621.6 0 53 261 56 621.6 0 56 262 104 621.6 0 104 263 92 621.6 0 92 264 89 621.6 0 89 265 82 621.6 0 82 266 82 621.6 0 82 267 76 621.6 0 76 268 80 621.6 0 80 269 70 621.6 0 70 270 48 621.6 0 48 271 47 621.6 0 47 272 46 621.6 0 46 273 57 621.6 0 57 274 53 621.6 0 53 275 50 621.6 0 50 276 66 621.6 0 66 277 54 621.6 0 54 278 47 621.6 0 47 279 34 621.6 0 34 280 59 621.6 0 59 281 60 621.6 0 60 282 63 621.6 0 63 283 62 621.6 0 62 284 49 621.6 0 49 285 45 621.6 0 45 286 58 621.6 0 58 287 49 621.6 0 49 288 51 621.6 0 51 289 50 621.6 0 50 290 43 621.6 0 43 291 41 621.6 0 41 292 46 621.6 0 46 293 64 621.6 0 64 294 57 621.6 0 57 295 51 621.6 0 51 296 47 621.6 0 47 297 48 621.6 0 48 298 63 621.6 0 63 299 51 621.6 0 51 300 53 621.6 0 53 301 44 621.6 0 44 Finished in 6.81 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 168,971 Reads with adapters: 7,576 (4.5%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 7,551 (4.5%) Total basepairs processed: 31,446,269 bp Total written (filtered): 2,048,752 bp (6.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7576 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 17 0.0 3 0 14 3 20 1343 0.0 4 1247 93 3 21 6114 0.0 4 6003 108 2 1 22 11 0.0 4 2 9 24 5 0.0 4 0 0 0 4 1 34 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 56 0.0 4 0 1 0 0 55 128 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 140 2 0.0 4 0 0 0 0 2 141 2 0.0 4 0 0 0 1 1 142 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 0 2 171 4 0.0 4 0 0 0 0 4 172 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 7,551 Reads with adapters: 2,079 (27.5%) Reads written (passing filters): 7,551 (100.0%) Total basepairs processed: 2,048,752 bp Total written (filtered): 2,028,942 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2079 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.5% C: 1.6% G: 96.7% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 118.0 0 2 4 2 29.5 0 2 9 1591 0.0 0 46 1545 10 264 0.0 1 5 259 11 217 0.0 1 1 216 12 1 0.0 1 0 1 170 1 0.0 1 1 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.