This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.09 s (23 us/read; 2.58 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 112,349 (3.6%) Reads that were too short: 39,385 (1.3%) Reads written (passing filters): 72,964 (2.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 15,951,788 bp (1.7%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 112349 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 263 755.5 0 263 7 8861 755.5 0 8861 8 117 755.5 0 117 9 187 755.5 0 187 10 179 755.5 0 179 11 171 755.5 0 171 12 456 755.5 0 456 13 13451 755.5 0 13451 14 8499 755.5 0 8499 15 23 755.5 0 23 16 8 755.5 0 8 17 9 755.5 0 9 18 9 755.5 0 9 19 4 755.5 0 4 20 8 755.5 0 8 21 6 755.5 0 6 22 12 755.5 0 12 23 6 755.5 0 6 24 7 755.5 0 7 25 11 755.5 0 11 26 19 755.5 0 19 27 23 755.5 0 23 28 8 755.5 0 8 29 9 755.5 0 9 30 8 755.5 0 8 31 12 755.5 0 12 32 8 755.5 0 8 33 5 755.5 0 5 34 9 755.5 0 9 35 11 755.5 0 11 36 12 755.5 0 12 37 5 755.5 0 5 38 3 755.5 0 3 39 9 755.5 0 9 40 105 755.5 0 105 41 8 755.5 0 8 42 4 755.5 0 4 43 144 755.5 0 144 44 18 755.5 0 18 45 12 755.5 0 12 46 10 755.5 0 10 47 3 755.5 0 3 48 10 755.5 0 10 49 5 755.5 0 5 50 6 755.5 0 6 51 4 755.5 0 4 52 8 755.5 0 8 53 6 755.5 0 6 54 10 755.5 0 10 55 6 755.5 0 6 56 4 755.5 0 4 57 14 755.5 0 14 58 10 755.5 0 10 59 7 755.5 0 7 60 7 755.5 0 7 61 10 755.5 0 10 62 4 755.5 0 4 63 21 755.5 0 21 64 3 755.5 0 3 65 15 755.5 0 15 66 9 755.5 0 9 67 9 755.5 0 9 68 5 755.5 0 5 69 3 755.5 0 3 70 5 755.5 0 5 71 15 755.5 0 15 72 28 755.5 0 28 73 284 755.5 0 284 74 40 755.5 0 40 75 50 755.5 0 50 76 24 755.5 0 24 77 118 755.5 0 118 78 69 755.5 0 69 79 145 755.5 0 145 80 111 755.5 0 111 81 113 755.5 0 113 82 76 755.5 0 76 83 94 755.5 0 94 84 80 755.5 0 80 85 31 755.5 0 31 86 114 755.5 0 114 87 116 755.5 0 116 88 30 755.5 0 30 89 171 755.5 0 171 90 374 755.5 0 374 91 342 755.5 0 342 92 111 755.5 0 111 93 156 755.5 0 156 94 238 755.5 0 238 95 323 755.5 0 323 96 507 755.5 0 507 97 244 755.5 0 244 98 101 755.5 0 101 99 26 755.5 0 26 100 74 755.5 0 74 101 106 755.5 0 106 102 52 755.5 0 52 103 133 755.5 0 133 104 221 755.5 0 221 105 160 755.5 0 160 106 2641 755.5 0 2641 107 535 755.5 0 535 108 216 755.5 0 216 109 95 755.5 0 95 110 484 755.5 0 484 111 298 755.5 0 298 112 339 755.5 0 339 113 360 755.5 0 360 114 123 755.5 0 123 115 117 755.5 0 117 116 94 755.5 0 94 117 75 755.5 0 75 118 75 755.5 0 75 119 154 755.5 0 154 120 604 755.5 0 604 121 253 755.5 0 253 122 463 755.5 0 463 123 185 755.5 0 185 124 141 755.5 0 141 125 182 755.5 0 182 126 316 755.5 0 316 127 1947 755.5 0 1947 128 1296 755.5 0 1296 129 779 755.5 0 779 130 164 755.5 0 164 131 103 755.5 0 103 132 134 755.5 0 134 133 182 755.5 0 182 134 316 755.5 0 316 135 607 755.5 0 607 136 418 755.5 0 418 137 296 755.5 0 296 138 382 755.5 0 382 139 1185 755.5 0 1185 140 1209 755.5 0 1209 141 489 755.5 0 489 142 542 755.5 0 542 143 151 755.5 0 151 144 152 755.5 0 152 145 335 755.5 0 335 146 677 755.5 0 677 147 290 755.5 0 290 148 346 755.5 0 346 149 127 755.5 0 127 150 144 755.5 0 144 151 708 755.5 0 708 152 341 755.5 0 341 153 741 755.5 0 741 154 387 755.5 0 387 155 371 755.5 0 371 156 175 755.5 0 175 157 174 755.5 0 174 158 453 755.5 0 453 159 277 755.5 0 277 160 398 755.5 0 398 161 1023 755.5 0 1023 162 238 755.5 0 238 163 284 755.5 0 284 164 164 755.5 0 164 165 314 755.5 0 314 166 270 755.5 0 270 167 252 755.5 0 252 168 160 755.5 0 160 169 103 755.5 0 103 170 200 755.5 0 200 171 125 755.5 0 125 172 172 755.5 0 172 173 267 755.5 0 267 174 707 755.5 0 707 175 244 755.5 0 244 176 211 755.5 0 211 177 129 755.5 0 129 178 111 755.5 0 111 179 228 755.5 0 228 180 244 755.5 0 244 181 381 755.5 0 381 182 346 755.5 0 346 183 356 755.5 0 356 184 281 755.5 0 281 185 281 755.5 0 281 186 260 755.5 0 260 187 198 755.5 0 198 188 125 755.5 0 125 189 98 755.5 0 98 190 235 755.5 0 235 191 348 755.5 0 348 192 612 755.5 0 612 193 210 755.5 0 210 194 299 755.5 0 299 195 148 755.5 0 148 196 82 755.5 0 82 197 132 755.5 0 132 198 435 755.5 0 435 199 422 755.5 0 422 200 265 755.5 0 265 201 168 755.5 0 168 202 239 755.5 0 239 203 362 755.5 0 362 204 358 755.5 0 358 205 174 755.5 0 174 206 338 755.5 0 338 207 312 755.5 0 312 208 269 755.5 0 269 209 232 755.5 0 232 210 333 755.5 0 333 211 345 755.5 0 345 212 177 755.5 0 177 213 187 755.5 0 187 214 358 755.5 0 358 215 302 755.5 0 302 216 233 755.5 0 233 217 162 755.5 0 162 218 216 755.5 0 216 219 340 755.5 0 340 220 541 755.5 0 541 221 210 755.5 0 210 222 129 755.5 0 129 223 160 755.5 0 160 224 433 755.5 0 433 225 332 755.5 0 332 226 304 755.5 0 304 227 322 755.5 0 322 228 293 755.5 0 293 229 196 755.5 0 196 230 190 755.5 0 190 231 190 755.5 0 190 232 200 755.5 0 200 233 273 755.5 0 273 234 292 755.5 0 292 235 366 755.5 0 366 236 322 755.5 0 322 237 390 755.5 0 390 238 176 755.5 0 176 239 135 755.5 0 135 240 355 755.5 0 355 241 362 755.5 0 362 242 343 755.5 0 343 243 405 755.5 0 405 244 342 755.5 0 342 245 485 755.5 0 485 246 330 755.5 0 330 247 277 755.5 0 277 248 155 755.5 0 155 249 127 755.5 0 127 250 100 755.5 0 100 251 222 755.5 0 222 252 231 755.5 0 231 253 407 755.5 0 407 254 334 755.5 0 334 255 421 755.5 0 421 256 744 755.5 0 744 257 480 755.5 0 480 258 350 755.5 0 350 259 126 755.5 0 126 260 166 755.5 0 166 261 168 755.5 0 168 262 186 755.5 0 186 263 184 755.5 0 184 264 304 755.5 0 304 265 312 755.5 0 312 266 607 755.5 0 607 267 1962 755.5 0 1962 268 764 755.5 0 764 269 752 755.5 0 752 270 142 755.5 0 142 271 239 755.5 0 239 272 269 755.5 0 269 273 250 755.5 0 250 274 268 755.5 0 268 275 202 755.5 0 202 276 216 755.5 0 216 277 458 755.5 0 458 278 463 755.5 0 463 279 823 755.5 0 823 280 1576 755.5 0 1576 281 2956 755.5 0 2956 282 1375 755.5 0 1375 283 566 755.5 0 566 284 456 755.5 0 456 285 490 755.5 0 490 286 406 755.5 0 406 287 758 755.5 0 758 288 731 755.5 0 731 289 321 755.5 0 321 290 195 755.5 0 195 291 166 755.5 0 166 292 306 755.5 0 306 293 493 755.5 0 493 294 554 755.5 0 554 295 320 755.5 0 320 296 397 755.5 0 397 297 367 755.5 0 367 298 257 755.5 0 257 299 397 755.5 0 397 300 299 755.5 0 299 301 277 755.5 0 277 Finished in 2.39 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 72,964 Reads with adapters: 9,063 (12.4%) Reads that were too short: 64 (0.1%) Reads written (passing filters): 8,999 (12.3%) Total basepairs processed: 15,951,788 bp Total written (filtered): 2,453,518 bp (15.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9063 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 61 0.0 3 0 59 1 1 17 8638 0.0 3 8473 143 14 8 18 29 0.0 3 0 27 2 19 17 0.0 3 1 0 14 2 20 5 0.0 3 1 1 0 3 21 1 0.0 3 1 22 1 0.0 3 1 23 1 0.0 3 0 0 1 32 9 0.0 3 0 0 0 9 35 2 0.0 3 0 0 1 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 53 8 0.0 3 0 0 0 8 56 3 0.0 3 0 0 0 3 58 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 21 0.0 3 0 0 0 21 68 2 0.0 3 0 0 0 2 70 5 0.0 3 0 0 0 5 72 1 0.0 3 0 0 1 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 110 8 0.0 3 0 0 0 8 115 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 6 0.0 3 0 0 0 6 159 19 0.0 3 0 0 0 19 160 11 0.0 3 0 0 0 11 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 13 0.0 3 0 0 0 13 179 85 0.0 3 0 0 0 85 180 6 0.0 3 0 0 0 6 182 26 0.0 3 0 0 0 26 183 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 4 0.0 3 0 0 0 4 189 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 255 2 0.0 3 0 0 0 2 262 1 0.0 3 0 0 0 1 271 4 0.0 3 0 0 0 4 272 7 0.0 3 0 0 0 7 273 3 0.0 3 0 0 0 3 274 4 0.0 3 0 0 0 4 275 16 0.0 3 0 0 2 14 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 8,999 Reads with adapters: 862 (9.6%) Reads written (passing filters): 8,999 (100.0%) Total basepairs processed: 2,453,518 bp Total written (filtered): 2,450,811 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 862 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.8% C: 12.8% G: 22.7% T: 16.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 752 140.6 0 752 4 103 35.2 0 103 5 5 8.8 0 5 6 1 2.2 0 1 8 1 0.1 0 1