This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.04 s (23 us/read; 2.61 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 428,631 (13.9%) Reads that were too short: 3,784 (0.1%) Reads written (passing filters): 424,847 (13.7%) Total basepairs processed: 931,490,553 bp Total written (filtered): 80,558,385 bp (8.6%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 428631 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 200 755.5 0 200 7 2584 755.5 0 2584 8 354 755.5 0 354 9 8410 755.5 0 8410 10 13 755.5 0 13 11 9 755.5 0 9 12 10 755.5 0 10 13 6 755.5 0 6 14 7 755.5 0 7 15 31 755.5 0 31 16 15 755.5 0 15 17 17 755.5 0 17 18 10 755.5 0 10 19 16 755.5 0 16 20 17 755.5 0 17 21 17 755.5 0 17 22 8 755.5 0 8 23 23 755.5 0 23 24 14 755.5 0 14 25 17 755.5 0 17 26 14 755.5 0 14 27 22 755.5 0 22 28 16 755.5 0 16 29 12 755.5 0 12 30 26 755.5 0 26 31 35 755.5 0 35 32 46 755.5 0 46 33 71 755.5 0 71 34 96 755.5 0 96 35 14 755.5 0 14 36 29 755.5 0 29 37 48 755.5 0 48 38 42 755.5 0 42 39 16 755.5 0 16 40 12 755.5 0 12 41 14 755.5 0 14 42 13 755.5 0 13 43 19 755.5 0 19 44 7 755.5 0 7 45 18 755.5 0 18 46 20 755.5 0 20 47 13 755.5 0 13 48 26 755.5 0 26 49 33 755.5 0 33 50 8 755.5 0 8 51 12 755.5 0 12 52 23 755.5 0 23 53 23 755.5 0 23 54 42 755.5 0 42 55 22 755.5 0 22 56 453 755.5 0 453 57 41 755.5 0 41 58 375 755.5 0 375 59 7716 755.5 0 7716 60 591 755.5 0 591 61 105 755.5 0 105 62 16 755.5 0 16 63 13 755.5 0 13 64 27 755.5 0 27 65 22 755.5 0 22 66 160 755.5 0 160 67 9 755.5 0 9 68 35 755.5 0 35 69 13 755.5 0 13 70 24 755.5 0 24 71 26 755.5 0 26 72 19 755.5 0 19 73 7 755.5 0 7 74 20 755.5 0 20 75 23 755.5 0 23 76 63 755.5 0 63 77 512 755.5 0 512 78 90 755.5 0 90 79 31 755.5 0 31 80 32 755.5 0 32 81 17 755.5 0 17 82 17 755.5 0 17 83 27 755.5 0 27 84 60 755.5 0 60 85 35 755.5 0 35 86 63 755.5 0 63 87 372 755.5 0 372 88 34 755.5 0 34 89 14 755.5 0 14 90 18 755.5 0 18 91 25 755.5 0 25 92 28 755.5 0 28 93 21 755.5 0 21 94 37 755.5 0 37 95 47 755.5 0 47 96 181 755.5 0 181 97 724 755.5 0 724 98 83 755.5 0 83 99 146 755.5 0 146 100 151 755.5 0 151 101 77 755.5 0 77 102 193 755.5 0 193 103 220 755.5 0 220 104 197 755.5 0 197 105 86 755.5 0 86 106 117 755.5 0 117 107 358 755.5 0 358 108 564 755.5 0 564 109 661 755.5 0 661 110 1230 755.5 0 1230 111 930 755.5 0 930 112 4845 755.5 0 4845 113 15763 755.5 0 15763 114 314355 755.5 0 314355 115 8210 755.5 0 8210 116 31079 755.5 0 31079 117 187 755.5 0 187 118 50 755.5 0 50 119 26 755.5 0 26 120 33 755.5 0 33 121 13 755.5 0 13 122 22 755.5 0 22 123 41 755.5 0 41 124 12 755.5 0 12 125 12 755.5 0 12 126 25 755.5 0 25 127 47 755.5 0 47 128 29 755.5 0 29 129 166 755.5 0 166 130 39 755.5 0 39 131 25 755.5 0 25 132 10 755.5 0 10 133 13 755.5 0 13 134 21 755.5 0 21 135 35 755.5 0 35 136 79 755.5 0 79 137 38 755.5 0 38 138 44 755.5 0 44 139 21 755.5 0 21 140 61 755.5 0 61 141 167 755.5 0 167 142 440 755.5 0 440 143 413 755.5 0 413 144 54 755.5 0 54 145 115 755.5 0 115 146 341 755.5 0 341 147 5460 755.5 0 5460 148 859 755.5 0 859 149 1448 755.5 0 1448 150 84 755.5 0 84 151 104 755.5 0 104 152 275 755.5 0 275 153 4208 755.5 0 4208 154 451 755.5 0 451 155 3867 755.5 0 3867 156 133 755.5 0 133 157 60 755.5 0 60 158 119 755.5 0 119 159 9 755.5 0 9 160 12 755.5 0 12 161 19 755.5 0 19 162 21 755.5 0 21 163 19 755.5 0 19 164 21 755.5 0 21 165 29 755.5 0 29 166 28 755.5 0 28 167 27 755.5 0 27 168 180 755.5 0 180 169 63 755.5 0 63 170 41 755.5 0 41 171 17 755.5 0 17 172 11 755.5 0 11 173 8 755.5 0 8 174 14 755.5 0 14 175 18 755.5 0 18 176 32 755.5 0 32 177 10 755.5 0 10 178 12 755.5 0 12 179 9 755.5 0 9 180 11 755.5 0 11 181 40 755.5 0 40 182 37 755.5 0 37 183 40 755.5 0 40 184 45 755.5 0 45 185 45 755.5 0 45 186 81 755.5 0 81 187 12 755.5 0 12 188 10 755.5 0 10 189 11 755.5 0 11 190 77 755.5 0 77 191 94 755.5 0 94 192 19 755.5 0 19 193 15 755.5 0 15 194 9 755.5 0 9 195 7 755.5 0 7 196 11 755.5 0 11 197 5 755.5 0 5 198 17 755.5 0 17 199 12 755.5 0 12 200 10 755.5 0 10 201 14 755.5 0 14 202 214 755.5 0 214 203 32 755.5 0 32 204 59 755.5 0 59 205 18 755.5 0 18 206 10 755.5 0 10 207 12 755.5 0 12 208 8 755.5 0 8 209 10 755.5 0 10 210 6 755.5 0 6 211 13 755.5 0 13 212 13 755.5 0 13 213 25 755.5 0 25 214 14 755.5 0 14 215 16 755.5 0 16 216 46 755.5 0 46 217 68 755.5 0 68 218 28 755.5 0 28 219 15 755.5 0 15 220 8 755.5 0 8 221 11 755.5 0 11 222 9 755.5 0 9 223 7 755.5 0 7 224 15 755.5 0 15 225 27 755.5 0 27 226 96 755.5 0 96 227 53 755.5 0 53 228 81 755.5 0 81 229 20 755.5 0 20 230 14 755.5 0 14 231 8 755.5 0 8 232 11 755.5 0 11 233 14 755.5 0 14 234 19 755.5 0 19 235 41 755.5 0 41 236 34 755.5 0 34 237 22 755.5 0 22 238 21 755.5 0 21 239 4 755.5 0 4 240 1 755.5 0 1 241 5 755.5 0 5 242 4 755.5 0 4 243 8 755.5 0 8 244 11 755.5 0 11 245 24 755.5 0 24 246 19 755.5 0 19 247 5 755.5 0 5 248 6 755.5 0 6 249 8 755.5 0 8 250 10 755.5 0 10 251 17 755.5 0 17 252 14 755.5 0 14 253 25 755.5 0 25 254 42 755.5 0 42 255 46 755.5 0 46 256 28 755.5 0 28 257 26 755.5 0 26 258 14 755.5 0 14 259 6 755.5 0 6 260 11 755.5 0 11 261 11 755.5 0 11 262 11 755.5 0 11 263 9 755.5 0 9 264 22 755.5 0 22 265 18 755.5 0 18 266 22 755.5 0 22 267 20 755.5 0 20 268 9 755.5 0 9 269 11 755.5 0 11 270 32 755.5 0 32 271 24 755.5 0 24 272 25 755.5 0 25 273 36 755.5 0 36 274 24 755.5 0 24 275 205 755.5 0 205 276 36 755.5 0 36 277 30 755.5 0 30 278 39 755.5 0 39 279 97 755.5 0 97 280 69 755.5 0 69 281 110 755.5 0 110 282 155 755.5 0 155 283 542 755.5 0 542 284 29 755.5 0 29 285 25 755.5 0 25 286 17 755.5 0 17 287 29 755.5 0 29 288 12 755.5 0 12 289 52 755.5 0 52 290 282 755.5 0 282 291 113 755.5 0 113 292 101 755.5 0 101 293 39 755.5 0 39 294 33 755.5 0 33 295 16 755.5 0 16 296 6 755.5 0 6 298 7 755.5 0 7 299 6 755.5 0 6 300 6 755.5 0 6 301 2 755.5 0 2 Finished in 13.95 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 424,847 Reads with adapters: 8,942 (2.1%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 8,940 (2.1%) Total basepairs processed: 80,558,385 bp Total written (filtered): 2,431,877 bp (3.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8942 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 0 2 18 8 0.0 3 0 0 5 0 3 19 119 0.0 3 0 116 1 1 1 20 8776 0.0 4 8646 124 3 1 2 21 22 0.0 4 3 16 0 2 1 22 2 0.0 4 0 0 1 0 1 23 4 0.0 4 0 0 0 4 25 1 0.0 4 0 1 100 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 8,940 Reads with adapters: 6,824 (76.3%) Reads written (passing filters): 8,940 (100.0%) Total basepairs processed: 2,431,877 bp Total written (filtered): 612,979 bp (25.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6824 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.1% G: 0.3% T: 99.5% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "T" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 2 0.0 0 0 2 10 8 0.0 1 0 8 11 5 0.0 1 0 5 238 1 0.0 1 1 256 1 0.0 1 1 263 1 0.0 1 1 265 1 0.0 1 1 266 16 0.0 1 16 267 6259 0.0 1 5908 351 268 346 0.0 1 337 9 269 100 0.0 1 94 6 270 80 0.0 1 80 271 3 0.0 1 3 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.