This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.63 s (23 us/read; 2.59 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 72,910 (2.4%) Reads that were too short: 25,579 (0.8%) Reads written (passing filters): 47,331 (1.5%) Total basepairs processed: 931,490,553 bp Total written (filtered): 10,363,673 bp (1.1%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 72910 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 196 755.5 0 196 7 9305 755.5 0 9305 8 18 755.5 0 18 9 16 755.5 0 16 10 8 755.5 0 8 11 8 755.5 0 8 12 12 755.5 0 12 13 2 755.5 0 2 14 10 755.5 0 10 15 5 755.5 0 5 16 14 755.5 0 14 17 14 755.5 0 14 18 8 755.5 0 8 19 13 755.5 0 13 20 30 755.5 0 30 21 78 755.5 0 78 22 24 755.5 0 24 23 14 755.5 0 14 24 37 755.5 0 37 25 33 755.5 0 33 26 22 755.5 0 22 27 73 755.5 0 73 28 272 755.5 0 272 29 86 755.5 0 86 30 35 755.5 0 35 31 57 755.5 0 57 32 103 755.5 0 103 33 135 755.5 0 135 34 6667 755.5 0 6667 35 50 755.5 0 50 36 61 755.5 0 61 37 11 755.5 0 11 38 20 755.5 0 20 39 15 755.5 0 15 40 37 755.5 0 37 41 37 755.5 0 37 42 68 755.5 0 68 43 2219 755.5 0 2219 44 609 755.5 0 609 45 17 755.5 0 17 46 12 755.5 0 12 47 17 755.5 0 17 48 13 755.5 0 13 49 7 755.5 0 7 50 27 755.5 0 27 51 8 755.5 0 8 52 12 755.5 0 12 53 18 755.5 0 18 54 14 755.5 0 14 55 14 755.5 0 14 56 20 755.5 0 20 57 25 755.5 0 25 58 590 755.5 0 590 59 37 755.5 0 37 60 29 755.5 0 29 61 12 755.5 0 12 62 10 755.5 0 10 63 8 755.5 0 8 64 9 755.5 0 9 65 15 755.5 0 15 66 17 755.5 0 17 67 26 755.5 0 26 68 47 755.5 0 47 69 44 755.5 0 44 70 90 755.5 0 90 71 76 755.5 0 76 72 1283 755.5 0 1283 73 556 755.5 0 556 74 913 755.5 0 913 75 38 755.5 0 38 76 177 755.5 0 177 77 215 755.5 0 215 78 27 755.5 0 27 79 77 755.5 0 77 80 119 755.5 0 119 81 83 755.5 0 83 82 106 755.5 0 106 83 163 755.5 0 163 84 91 755.5 0 91 85 21 755.5 0 21 86 63 755.5 0 63 87 52 755.5 0 52 88 130 755.5 0 130 89 604 755.5 0 604 90 629 755.5 0 629 91 90 755.5 0 90 92 933 755.5 0 933 93 109 755.5 0 109 94 53 755.5 0 53 95 130 755.5 0 130 96 76 755.5 0 76 97 138 755.5 0 138 98 45 755.5 0 45 99 44 755.5 0 44 100 33 755.5 0 33 101 22 755.5 0 22 102 15 755.5 0 15 103 30 755.5 0 30 104 31 755.5 0 31 105 19 755.5 0 19 106 49 755.5 0 49 107 36 755.5 0 36 108 29 755.5 0 29 109 183 755.5 0 183 110 169 755.5 0 169 111 37 755.5 0 37 112 91 755.5 0 91 113 179 755.5 0 179 114 672 755.5 0 672 115 109 755.5 0 109 116 56 755.5 0 56 117 48 755.5 0 48 118 69 755.5 0 69 119 24 755.5 0 24 120 48 755.5 0 48 121 89 755.5 0 89 122 100 755.5 0 100 123 69 755.5 0 69 124 71 755.5 0 71 125 41 755.5 0 41 126 50 755.5 0 50 127 81 755.5 0 81 128 140 755.5 0 140 129 117 755.5 0 117 130 197 755.5 0 197 131 130 755.5 0 130 132 69 755.5 0 69 133 93 755.5 0 93 134 146 755.5 0 146 135 155 755.5 0 155 136 237 755.5 0 237 137 145 755.5 0 145 138 116 755.5 0 116 139 104 755.5 0 104 140 121 755.5 0 121 141 222 755.5 0 222 142 199 755.5 0 199 143 2194 755.5 0 2194 144 1197 755.5 0 1197 145 4636 755.5 0 4636 146 445 755.5 0 445 147 76 755.5 0 76 148 90 755.5 0 90 149 72 755.5 0 72 150 97 755.5 0 97 151 41 755.5 0 41 152 41 755.5 0 41 153 38 755.5 0 38 154 75 755.5 0 75 155 123 755.5 0 123 156 330 755.5 0 330 157 171 755.5 0 171 158 84 755.5 0 84 159 77 755.5 0 77 160 64 755.5 0 64 161 88 755.5 0 88 162 90 755.5 0 90 163 129 755.5 0 129 164 102 755.5 0 102 165 249 755.5 0 249 166 118 755.5 0 118 167 141 755.5 0 141 168 130 755.5 0 130 169 80 755.5 0 80 170 91 755.5 0 91 171 40 755.5 0 40 172 36 755.5 0 36 173 76 755.5 0 76 174 92 755.5 0 92 175 122 755.5 0 122 176 93 755.5 0 93 177 77 755.5 0 77 178 32 755.5 0 32 179 66 755.5 0 66 180 54 755.5 0 54 181 75 755.5 0 75 182 116 755.5 0 116 183 110 755.5 0 110 184 143 755.5 0 143 185 76 755.5 0 76 186 104 755.5 0 104 187 94 755.5 0 94 188 73 755.5 0 73 189 61 755.5 0 61 190 142 755.5 0 142 191 109 755.5 0 109 192 129 755.5 0 129 193 97 755.5 0 97 194 209 755.5 0 209 195 185 755.5 0 185 196 118 755.5 0 118 197 157 755.5 0 157 198 108 755.5 0 108 199 73 755.5 0 73 200 82 755.5 0 82 201 71 755.5 0 71 202 220 755.5 0 220 203 384 755.5 0 384 204 197 755.5 0 197 205 121 755.5 0 121 206 181 755.5 0 181 207 154 755.5 0 154 208 416 755.5 0 416 209 395 755.5 0 395 210 508 755.5 0 508 211 198 755.5 0 198 212 218 755.5 0 218 213 213 755.5 0 213 214 674 755.5 0 674 215 757 755.5 0 757 216 246 755.5 0 246 217 206 755.5 0 206 218 113 755.5 0 113 219 73 755.5 0 73 220 73 755.5 0 73 221 80 755.5 0 80 222 163 755.5 0 163 223 316 755.5 0 316 224 94 755.5 0 94 225 86 755.5 0 86 226 193 755.5 0 193 227 120 755.5 0 120 228 169 755.5 0 169 229 121 755.5 0 121 230 161 755.5 0 161 231 261 755.5 0 261 232 128 755.5 0 128 233 175 755.5 0 175 234 174 755.5 0 174 235 153 755.5 0 153 236 256 755.5 0 256 237 178 755.5 0 178 238 134 755.5 0 134 239 93 755.5 0 93 240 101 755.5 0 101 241 88 755.5 0 88 242 119 755.5 0 119 243 161 755.5 0 161 244 275 755.5 0 275 245 125 755.5 0 125 246 111 755.5 0 111 247 118 755.5 0 118 248 120 755.5 0 120 249 105 755.5 0 105 250 99 755.5 0 99 251 67 755.5 0 67 252 82 755.5 0 82 253 89 755.5 0 89 254 57 755.5 0 57 255 90 755.5 0 90 256 101 755.5 0 101 257 143 755.5 0 143 258 117 755.5 0 117 259 143 755.5 0 143 260 136 755.5 0 136 261 161 755.5 0 161 262 71 755.5 0 71 263 68 755.5 0 68 264 63 755.5 0 63 265 112 755.5 0 112 266 135 755.5 0 135 267 353 755.5 0 353 268 201 755.5 0 201 269 115 755.5 0 115 270 195 755.5 0 195 271 167 755.5 0 167 272 107 755.5 0 107 273 140 755.5 0 140 274 155 755.5 0 155 275 148 755.5 0 148 276 137 755.5 0 137 277 86 755.5 0 86 278 159 755.5 0 159 279 152 755.5 0 152 280 2039 755.5 0 2039 281 1000 755.5 0 1000 282 3199 755.5 0 3199 283 234 755.5 0 234 284 95 755.5 0 95 285 126 755.5 0 126 286 134 755.5 0 134 287 211 755.5 0 211 288 431 755.5 0 431 289 249 755.5 0 249 290 121 755.5 0 121 291 110 755.5 0 110 292 109 755.5 0 109 293 264 755.5 0 264 294 205 755.5 0 205 295 245 755.5 0 245 296 376 755.5 0 376 297 375 755.5 0 375 298 446 755.5 0 446 299 1340 755.5 0 1340 300 592 755.5 0 592 301 104 755.5 0 104 Finished in 1.57 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 47,331 Reads with adapters: 9,510 (20.1%) Reads that were too short: 29 (0.1%) Reads written (passing filters): 9,481 (20.0%) Total basepairs processed: 10,363,673 bp Total written (filtered): 2,617,326 bp (25.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9510 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 2 1 16 84 0.0 3 0 75 4 5 17 9260 0.0 3 8878 333 38 11 18 28 0.0 3 2 17 8 1 19 37 0.0 3 17 2 7 11 20 7 0.0 3 0 0 0 7 21 2 0.0 3 0 0 1 1 22 1 0.0 3 1 32 1 0.0 3 0 0 0 1 37 3 0.0 3 1 0 0 2 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 49 2 0.0 3 1 1 50 2 0.0 3 1 0 0 1 51 3 0.0 3 1 0 0 2 59 4 0.0 3 0 0 0 4 71 1 0.0 3 0 0 0 1 79 6 0.0 3 0 0 1 5 81 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 100 16 0.0 3 0 0 0 16 101 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 1 1 105 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 150 15 0.0 3 0 0 0 15 152 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (30 us/read; 2.03 M reads/minute). === Summary === Total reads processed: 9,481 Reads with adapters: 220 (2.3%) Reads written (passing filters): 9,481 (100.0%) Total basepairs processed: 2,617,326 bp Total written (filtered): 2,616,629 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 220 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.2% C: 40.9% G: 4.5% T: 6.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 201 148.1 0 201 4 5 37.0 0 5 5 10 9.3 0 10 6 4 2.3 0 4