This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.92 s (23 us/read; 2.58 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 174,421 (5.6%) Reads that were too short: 24,796 (0.8%) Reads written (passing filters): 149,625 (4.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 28,192,725 bp (3.0%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 174421 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 166 755.5 0 166 7 9052 755.5 0 9052 8 9797 755.5 0 9797 9 19 755.5 0 19 10 10 755.5 0 10 11 7 755.5 0 7 12 26 755.5 0 26 13 11 755.5 0 11 14 250 755.5 0 250 15 35 755.5 0 35 16 4 755.5 0 4 17 7 755.5 0 7 18 4 755.5 0 4 19 5 755.5 0 5 20 7 755.5 0 7 21 13 755.5 0 13 22 5 755.5 0 5 23 3 755.5 0 3 24 9 755.5 0 9 25 17 755.5 0 17 26 66 755.5 0 66 27 75 755.5 0 75 28 6 755.5 0 6 29 4 755.5 0 4 30 9 755.5 0 9 31 12 755.5 0 12 32 116 755.5 0 116 33 61 755.5 0 61 34 7 755.5 0 7 35 6 755.5 0 6 36 15 755.5 0 15 37 6 755.5 0 6 38 9 755.5 0 9 39 7 755.5 0 7 40 50 755.5 0 50 41 7 755.5 0 7 42 7 755.5 0 7 43 12 755.5 0 12 44 28 755.5 0 28 45 7 755.5 0 7 46 4 755.5 0 4 47 10 755.5 0 10 48 14 755.5 0 14 49 7 755.5 0 7 50 11 755.5 0 11 51 11 755.5 0 11 52 14 755.5 0 14 53 7 755.5 0 7 54 14 755.5 0 14 55 19 755.5 0 19 56 10 755.5 0 10 57 36 755.5 0 36 58 2 755.5 0 2 59 10 755.5 0 10 60 15 755.5 0 15 61 10 755.5 0 10 62 21 755.5 0 21 63 8 755.5 0 8 64 10 755.5 0 10 65 24 755.5 0 24 66 11 755.5 0 11 67 20 755.5 0 20 68 11 755.5 0 11 69 4 755.5 0 4 70 14 755.5 0 14 71 7 755.5 0 7 72 27 755.5 0 27 73 30 755.5 0 30 74 27 755.5 0 27 75 16 755.5 0 16 76 10 755.5 0 10 77 15 755.5 0 15 78 28 755.5 0 28 79 26 755.5 0 26 80 55 755.5 0 55 81 80 755.5 0 80 82 34 755.5 0 34 83 40 755.5 0 40 84 52 755.5 0 52 85 30 755.5 0 30 86 52 755.5 0 52 87 151 755.5 0 151 88 166 755.5 0 166 89 123 755.5 0 123 90 289 755.5 0 289 91 583 755.5 0 583 92 153 755.5 0 153 93 153 755.5 0 153 94 451 755.5 0 451 95 251 755.5 0 251 96 181 755.5 0 181 97 332 755.5 0 332 98 315 755.5 0 315 99 352 755.5 0 352 100 317 755.5 0 317 101 447 755.5 0 447 102 564 755.5 0 564 103 785 755.5 0 785 104 12528 755.5 0 12528 105 17902 755.5 0 17902 106 35996 755.5 0 35996 107 201 755.5 0 201 108 34 755.5 0 34 109 13 755.5 0 13 110 41 755.5 0 41 111 38 755.5 0 38 112 60 755.5 0 60 113 32 755.5 0 32 114 65 755.5 0 65 115 35 755.5 0 35 116 24 755.5 0 24 117 30 755.5 0 30 118 24 755.5 0 24 119 225 755.5 0 225 120 138 755.5 0 138 121 88 755.5 0 88 122 62 755.5 0 62 123 55 755.5 0 55 124 60 755.5 0 60 125 52 755.5 0 52 126 72 755.5 0 72 127 56 755.5 0 56 128 76 755.5 0 76 129 138 755.5 0 138 130 79 755.5 0 79 131 54 755.5 0 54 132 206 755.5 0 206 133 61 755.5 0 61 134 52 755.5 0 52 135 29 755.5 0 29 136 40 755.5 0 40 137 55 755.5 0 55 138 181 755.5 0 181 139 141 755.5 0 141 140 82 755.5 0 82 141 119 755.5 0 119 142 252 755.5 0 252 143 81 755.5 0 81 144 115 755.5 0 115 145 127 755.5 0 127 146 87 755.5 0 87 147 123 755.5 0 123 148 93 755.5 0 93 149 81 755.5 0 81 150 56 755.5 0 56 151 91 755.5 0 91 152 136 755.5 0 136 153 199 755.5 0 199 154 214 755.5 0 214 155 315 755.5 0 315 156 264 755.5 0 264 157 667 755.5 0 667 158 1970 755.5 0 1970 159 29163 755.5 0 29163 160 7365 755.5 0 7365 161 7540 755.5 0 7540 162 104 755.5 0 104 163 105 755.5 0 105 164 66 755.5 0 66 165 83 755.5 0 83 166 61 755.5 0 61 167 166 755.5 0 166 168 113 755.5 0 113 169 135 755.5 0 135 170 59 755.5 0 59 171 155 755.5 0 155 172 112 755.5 0 112 173 68 755.5 0 68 174 70 755.5 0 70 175 37 755.5 0 37 176 35 755.5 0 35 177 51 755.5 0 51 178 49 755.5 0 49 179 79 755.5 0 79 180 120 755.5 0 120 181 128 755.5 0 128 182 107 755.5 0 107 183 108 755.5 0 108 184 121 755.5 0 121 185 115 755.5 0 115 186 91 755.5 0 91 187 347 755.5 0 347 188 137 755.5 0 137 189 92 755.5 0 92 190 83 755.5 0 83 191 51 755.5 0 51 192 67 755.5 0 67 193 24 755.5 0 24 194 40 755.5 0 40 195 72 755.5 0 72 196 69 755.5 0 69 197 35 755.5 0 35 198 96 755.5 0 96 199 103 755.5 0 103 200 1130 755.5 0 1130 201 442 755.5 0 442 202 252 755.5 0 252 203 63 755.5 0 63 204 175 755.5 0 175 205 109 755.5 0 109 206 89 755.5 0 89 207 106 755.5 0 106 208 103 755.5 0 103 209 56 755.5 0 56 210 84 755.5 0 84 211 83 755.5 0 83 212 77 755.5 0 77 213 77 755.5 0 77 214 92 755.5 0 92 215 136 755.5 0 136 216 111 755.5 0 111 217 130 755.5 0 130 218 88 755.5 0 88 219 103 755.5 0 103 220 151 755.5 0 151 221 249 755.5 0 249 222 68 755.5 0 68 223 74 755.5 0 74 224 188 755.5 0 188 225 150 755.5 0 150 226 117 755.5 0 117 227 101 755.5 0 101 228 174 755.5 0 174 229 96 755.5 0 96 230 97 755.5 0 97 231 70 755.5 0 70 232 93 755.5 0 93 233 76 755.5 0 76 234 37 755.5 0 37 235 103 755.5 0 103 236 165 755.5 0 165 237 84 755.5 0 84 238 178 755.5 0 178 239 389 755.5 0 389 240 6620 755.5 0 6620 241 4636 755.5 0 4636 242 776 755.5 0 776 243 206 755.5 0 206 244 125 755.5 0 125 245 97 755.5 0 97 246 78 755.5 0 78 247 77 755.5 0 77 248 155 755.5 0 155 249 106 755.5 0 106 250 126 755.5 0 126 251 74 755.5 0 74 252 101 755.5 0 101 253 195 755.5 0 195 254 249 755.5 0 249 255 201 755.5 0 201 256 114 755.5 0 114 257 82 755.5 0 82 258 79 755.5 0 79 259 50 755.5 0 50 260 60 755.5 0 60 261 80 755.5 0 80 262 70 755.5 0 70 263 139 755.5 0 139 264 190 755.5 0 190 265 164 755.5 0 164 266 110 755.5 0 110 267 79 755.5 0 79 268 107 755.5 0 107 269 166 755.5 0 166 270 98 755.5 0 98 271 108 755.5 0 108 272 98 755.5 0 98 273 177 755.5 0 177 274 148 755.5 0 148 275 160 755.5 0 160 276 129 755.5 0 129 277 130 755.5 0 130 278 155 755.5 0 155 279 178 755.5 0 178 280 305 755.5 0 305 281 264 755.5 0 264 282 154 755.5 0 154 283 309 755.5 0 309 284 143 755.5 0 143 285 113 755.5 0 113 286 91 755.5 0 91 287 62 755.5 0 62 288 81 755.5 0 81 289 80 755.5 0 80 290 83 755.5 0 83 291 110 755.5 0 110 292 67 755.5 0 67 293 82 755.5 0 82 294 100 755.5 0 100 295 56 755.5 0 56 296 25 755.5 0 25 297 63 755.5 0 63 298 121 755.5 0 121 299 218 755.5 0 218 300 599 755.5 0 599 301 383 755.5 0 383 Finished in 5.29 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 149,625 Reads with adapters: 15,681 (10.5%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 15,677 (10.5%) Total basepairs processed: 28,192,725 bp Total written (filtered): 3,684,568 bp (13.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15681 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 7 0.0 3 0 0 5 1 1 19 113 0.0 3 0 110 1 0 2 20 9972 0.0 4 9835 135 2 21 23 0.0 4 3 18 1 1 22 8 0.0 4 2 0 4 0 2 23 5 0.0 4 0 0 0 4 1 29 3 0.0 4 0 0 0 0 3 30 4123 0.0 4 0 0 0 0 4123 33 1 0.0 4 0 0 0 0 1 54 135 0.0 4 0 0 0 0 135 55 21 0.0 4 0 0 0 0 21 56 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 5 0.0 4 0 0 0 0 5 126 17 0.0 4 0 0 0 0 17 127 1235 0.0 4 0 0 0 0 1235 128 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 15,677 Reads with adapters: 189 (1.2%) Reads written (passing filters): 15,677 (100.0%) Total basepairs processed: 3,684,568 bp Total written (filtered): 3,682,213 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 189 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 18.0% C: 0.5% G: 79.9% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 245.0 0 6 4 24 61.2 0 24 5 3 15.3 0 3 10 1 0.0 1 0 1 11 152 0.0 1 1 151 12 1 0.0 1 1 260 1 0.0 1 1 272 1 0.0 1 1