This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.29 s (23 us/read; 2.57 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 195,193 (6.3%) Reads that were too short: 42,415 (1.4%) Reads written (passing filters): 152,778 (4.9%) Total basepairs processed: 931,490,553 bp Total written (filtered): 28,922,298 bp (3.1%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 195193 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 199 755.5 0 199 7 10049 755.5 0 10049 8 10035 755.5 0 10035 9 19 755.5 0 19 10 11 755.5 0 11 11 17 755.5 0 17 12 82 755.5 0 82 13 34 755.5 0 34 14 234 755.5 0 234 15 7 755.5 0 7 16 7 755.5 0 7 17 4 755.5 0 4 18 10 755.5 0 10 19 3 755.5 0 3 20 11 755.5 0 11 21 11 755.5 0 11 22 8 755.5 0 8 23 14 755.5 0 14 24 10 755.5 0 10 25 39 755.5 0 39 26 53 755.5 0 53 27 145 755.5 0 145 28 10 755.5 0 10 29 3 755.5 0 3 30 12 755.5 0 12 31 9 755.5 0 9 32 9 755.5 0 9 33 9 755.5 0 9 34 8 755.5 0 8 35 12 755.5 0 12 36 20 755.5 0 20 37 11 755.5 0 11 38 12 755.5 0 12 39 10 755.5 0 10 40 18 755.5 0 18 41 13 755.5 0 13 42 5 755.5 0 5 43 20 755.5 0 20 44 12 755.5 0 12 45 10 755.5 0 10 46 8 755.5 0 8 47 11 755.5 0 11 48 12 755.5 0 12 49 13 755.5 0 13 50 15 755.5 0 15 51 14 755.5 0 14 52 1 755.5 0 1 53 7 755.5 0 7 54 16 755.5 0 16 55 14 755.5 0 14 56 42 755.5 0 42 57 13 755.5 0 13 58 2 755.5 0 2 59 14 755.5 0 14 60 11 755.5 0 11 61 11 755.5 0 11 62 25 755.5 0 25 63 13 755.5 0 13 64 13 755.5 0 13 65 53 755.5 0 53 66 37 755.5 0 37 67 39 755.5 0 39 68 10 755.5 0 10 69 17 755.5 0 17 70 18 755.5 0 18 71 10 755.5 0 10 72 16 755.5 0 16 73 37 755.5 0 37 74 33 755.5 0 33 75 25 755.5 0 25 76 12 755.5 0 12 77 58 755.5 0 58 78 55 755.5 0 55 79 86 755.5 0 86 80 87 755.5 0 87 81 155 755.5 0 155 82 57 755.5 0 57 83 71 755.5 0 71 84 114 755.5 0 114 85 32 755.5 0 32 86 120 755.5 0 120 87 289 755.5 0 289 88 242 755.5 0 242 89 193 755.5 0 193 90 505 755.5 0 505 91 699 755.5 0 699 92 179 755.5 0 179 93 323 755.5 0 323 94 484 755.5 0 484 95 266 755.5 0 266 96 246 755.5 0 246 97 334 755.5 0 334 98 270 755.5 0 270 99 372 755.5 0 372 100 341 755.5 0 341 101 595 755.5 0 595 102 614 755.5 0 614 103 736 755.5 0 736 104 12429 755.5 0 12429 105 17088 755.5 0 17088 106 36739 755.5 0 36739 107 197 755.5 0 197 108 38 755.5 0 38 109 18 755.5 0 18 110 61 755.5 0 61 111 93 755.5 0 93 112 135 755.5 0 135 113 115 755.5 0 115 114 95 755.5 0 95 115 48 755.5 0 48 116 56 755.5 0 56 117 98 755.5 0 98 118 63 755.5 0 63 119 278 755.5 0 278 120 328 755.5 0 328 121 134 755.5 0 134 122 128 755.5 0 128 123 70 755.5 0 70 124 121 755.5 0 121 125 124 755.5 0 124 126 147 755.5 0 147 127 114 755.5 0 114 128 152 755.5 0 152 129 213 755.5 0 213 130 103 755.5 0 103 131 77 755.5 0 77 132 219 755.5 0 219 133 127 755.5 0 127 134 120 755.5 0 120 135 112 755.5 0 112 136 87 755.5 0 87 137 118 755.5 0 118 138 252 755.5 0 252 139 216 755.5 0 216 140 142 755.5 0 142 141 117 755.5 0 117 142 299 755.5 0 299 143 110 755.5 0 110 144 173 755.5 0 173 145 227 755.5 0 227 146 202 755.5 0 202 147 179 755.5 0 179 148 136 755.5 0 136 149 99 755.5 0 99 150 91 755.5 0 91 151 166 755.5 0 166 152 212 755.5 0 212 153 330 755.5 0 330 154 345 755.5 0 345 155 373 755.5 0 373 156 291 755.5 0 291 157 675 755.5 0 675 158 1338 755.5 0 1338 159 26071 755.5 0 26071 160 6226 755.5 0 6226 161 6385 755.5 0 6385 162 198 755.5 0 198 163 201 755.5 0 201 164 179 755.5 0 179 165 129 755.5 0 129 166 124 755.5 0 124 167 266 755.5 0 266 168 222 755.5 0 222 169 199 755.5 0 199 170 118 755.5 0 118 171 209 755.5 0 209 172 226 755.5 0 226 173 166 755.5 0 166 174 150 755.5 0 150 175 71 755.5 0 71 176 72 755.5 0 72 177 96 755.5 0 96 178 141 755.5 0 141 179 179 755.5 0 179 180 242 755.5 0 242 181 292 755.5 0 292 182 220 755.5 0 220 183 194 755.5 0 194 184 222 755.5 0 222 185 224 755.5 0 224 186 129 755.5 0 129 187 356 755.5 0 356 188 226 755.5 0 226 189 168 755.5 0 168 190 210 755.5 0 210 191 121 755.5 0 121 192 158 755.5 0 158 193 68 755.5 0 68 194 63 755.5 0 63 195 163 755.5 0 163 196 171 755.5 0 171 197 101 755.5 0 101 198 120 755.5 0 120 199 171 755.5 0 171 200 1526 755.5 0 1526 201 697 755.5 0 697 202 346 755.5 0 346 203 133 755.5 0 133 204 235 755.5 0 235 205 222 755.5 0 222 206 175 755.5 0 175 207 216 755.5 0 216 208 276 755.5 0 276 209 133 755.5 0 133 210 198 755.5 0 198 211 138 755.5 0 138 212 162 755.5 0 162 213 152 755.5 0 152 214 143 755.5 0 143 215 254 755.5 0 254 216 237 755.5 0 237 217 231 755.5 0 231 218 184 755.5 0 184 219 216 755.5 0 216 220 280 755.5 0 280 221 601 755.5 0 601 222 180 755.5 0 180 223 149 755.5 0 149 224 335 755.5 0 335 225 248 755.5 0 248 226 216 755.5 0 216 227 187 755.5 0 187 228 462 755.5 0 462 229 239 755.5 0 239 230 171 755.5 0 171 231 134 755.5 0 134 232 190 755.5 0 190 233 126 755.5 0 126 234 91 755.5 0 91 235 196 755.5 0 196 236 253 755.5 0 253 237 135 755.5 0 135 238 359 755.5 0 359 239 605 755.5 0 605 240 10284 755.5 0 10284 241 5085 755.5 0 5085 242 1050 755.5 0 1050 243 280 755.5 0 280 244 263 755.5 0 263 245 287 755.5 0 287 246 174 755.5 0 174 247 171 755.5 0 171 248 324 755.5 0 324 249 208 755.5 0 208 250 266 755.5 0 266 251 214 755.5 0 214 252 251 755.5 0 251 253 396 755.5 0 396 254 855 755.5 0 855 255 684 755.5 0 684 256 227 755.5 0 227 257 156 755.5 0 156 258 124 755.5 0 124 259 84 755.5 0 84 260 84 755.5 0 84 261 76 755.5 0 76 262 105 755.5 0 105 263 195 755.5 0 195 264 337 755.5 0 337 265 269 755.5 0 269 266 201 755.5 0 201 267 157 755.5 0 157 268 232 755.5 0 232 269 212 755.5 0 212 270 193 755.5 0 193 271 252 755.5 0 252 272 194 755.5 0 194 273 189 755.5 0 189 274 219 755.5 0 219 275 245 755.5 0 245 276 215 755.5 0 215 277 235 755.5 0 235 278 261 755.5 0 261 279 379 755.5 0 379 280 558 755.5 0 558 281 341 755.5 0 341 282 353 755.5 0 353 283 836 755.5 0 836 284 226 755.5 0 226 285 249 755.5 0 249 286 200 755.5 0 200 287 170 755.5 0 170 288 100 755.5 0 100 289 147 755.5 0 147 290 274 755.5 0 274 291 407 755.5 0 407 292 242 755.5 0 242 293 212 755.5 0 212 294 251 755.5 0 251 295 137 755.5 0 137 296 121 755.5 0 121 297 403 755.5 0 403 298 420 755.5 0 420 299 1156 755.5 0 1156 300 769 755.5 0 769 301 402 755.5 0 402 Finished in 4.61 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 152,778 Reads with adapters: 10,127 (6.6%) Reads that were too short: 117 (0.1%) Reads written (passing filters): 10,010 (6.6%) Total basepairs processed: 28,922,298 bp Total written (filtered): 2,750,018 bp (9.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10127 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 3 1 16 103 0.0 3 0 96 4 3 17 9725 0.0 3 9352 315 47 11 18 22 0.0 3 2 17 3 19 14 0.0 3 0 1 7 6 20 6 0.0 3 0 1 0 5 21 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 26 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 46 26 0.0 3 0 0 0 26 51 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 40 0.0 3 0 0 0 40 88 15 0.0 3 0 0 0 15 89 1 0.0 3 0 0 0 1 90 25 0.0 3 0 0 1 24 91 5 0.0 3 0 0 0 5 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 100 4 0.0 3 0 0 0 4 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 11 0.0 3 0 0 0 11 131 27 0.0 3 0 0 0 27 132 1 0.0 3 0 0 1 134 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 2 0.0 3 0 0 0 2 149 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 35 0.0 3 0 0 1 34 190 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 6 0.0 3 0 0 0 6 282 5 0.0 3 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 10,010 Reads with adapters: 409 (4.1%) Reads written (passing filters): 10,010 (100.0%) Total basepairs processed: 2,750,018 bp Total written (filtered): 2,748,762 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 409 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.1% C: 13.0% G: 1.0% T: 13.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 393 156.4 0 393 4 10 39.1 0 10 6 5 2.4 0 5 7 1 0.6 0 1