This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.99 s (23 us/read; 2.58 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 265,659 (8.6%) Reads that were too short: 66,934 (2.2%) Reads written (passing filters): 198,725 (6.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 37,066,089 bp (4.0%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 265659 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 219 755.5 0 219 7 571 755.5 0 571 8 21658 755.5 0 21658 9 29 755.5 0 29 10 7 755.5 0 7 11 7 755.5 0 7 12 6 755.5 0 6 13 12 755.5 0 12 14 8 755.5 0 8 15 11 755.5 0 11 16 5 755.5 0 5 17 7 755.5 0 7 18 11 755.5 0 11 19 6 755.5 0 6 20 7 755.5 0 7 21 4 755.5 0 4 22 6 755.5 0 6 23 9 755.5 0 9 24 10 755.5 0 10 25 9 755.5 0 9 26 10 755.5 0 10 27 12 755.5 0 12 28 52 755.5 0 52 29 613 755.5 0 613 30 2 755.5 0 2 31 17 755.5 0 17 32 8 755.5 0 8 33 10 755.5 0 10 34 12 755.5 0 12 35 12 755.5 0 12 36 26 755.5 0 26 37 11 755.5 0 11 38 9 755.5 0 9 39 15 755.5 0 15 40 13 755.5 0 13 41 9 755.5 0 9 42 18 755.5 0 18 43 15 755.5 0 15 44 9 755.5 0 9 45 12 755.5 0 12 46 10 755.5 0 10 47 10 755.5 0 10 48 11 755.5 0 11 49 6 755.5 0 6 50 9 755.5 0 9 51 9 755.5 0 9 52 12 755.5 0 12 53 15 755.5 0 15 54 6 755.5 0 6 55 8 755.5 0 8 56 4 755.5 0 4 57 15 755.5 0 15 58 14 755.5 0 14 59 22 755.5 0 22 60 26 755.5 0 26 61 19 755.5 0 19 62 9 755.5 0 9 63 7 755.5 0 7 64 26 755.5 0 26 65 21 755.5 0 21 66 16 755.5 0 16 67 14 755.5 0 14 68 17 755.5 0 17 69 35 755.5 0 35 70 442 755.5 0 442 71 128 755.5 0 128 72 214 755.5 0 214 73 44 755.5 0 44 74 10 755.5 0 10 75 18 755.5 0 18 76 8 755.5 0 8 77 21 755.5 0 21 78 38 755.5 0 38 79 151 755.5 0 151 80 120 755.5 0 120 81 207 755.5 0 207 82 361 755.5 0 361 83 542 755.5 0 542 84 41 755.5 0 41 85 159 755.5 0 159 86 48 755.5 0 48 87 11 755.5 0 11 88 48 755.5 0 48 89 96 755.5 0 96 90 9 755.5 0 9 91 7 755.5 0 7 92 273 755.5 0 273 93 267 755.5 0 267 94 212 755.5 0 212 95 215 755.5 0 215 96 97 755.5 0 97 97 33 755.5 0 33 98 325 755.5 0 325 99 996 755.5 0 996 100 359 755.5 0 359 101 77 755.5 0 77 102 203 755.5 0 203 103 45 755.5 0 45 104 97 755.5 0 97 105 270 755.5 0 270 106 116 755.5 0 116 107 136 755.5 0 136 108 247 755.5 0 247 109 608 755.5 0 608 110 371 755.5 0 371 111 929 755.5 0 929 112 242 755.5 0 242 113 319 755.5 0 319 114 874 755.5 0 874 115 530 755.5 0 530 116 494 755.5 0 494 117 1212 755.5 0 1212 118 796 755.5 0 796 119 557 755.5 0 557 120 757 755.5 0 757 121 990 755.5 0 990 122 1293 755.5 0 1293 123 1522 755.5 0 1522 124 28991 755.5 0 28991 125 23976 755.5 0 23976 126 76786 755.5 0 76786 127 865 755.5 0 865 128 100 755.5 0 100 129 497 755.5 0 497 130 389 755.5 0 389 131 306 755.5 0 306 132 143 755.5 0 143 133 196 755.5 0 196 134 238 755.5 0 238 135 301 755.5 0 301 136 288 755.5 0 288 137 274 755.5 0 274 138 248 755.5 0 248 139 349 755.5 0 349 140 882 755.5 0 882 141 160 755.5 0 160 142 478 755.5 0 478 143 337 755.5 0 337 144 439 755.5 0 439 145 320 755.5 0 320 146 193 755.5 0 193 147 188 755.5 0 188 148 223 755.5 0 223 149 1067 755.5 0 1067 150 1938 755.5 0 1938 151 3394 755.5 0 3394 152 209 755.5 0 209 153 205 755.5 0 205 154 163 755.5 0 163 155 173 755.5 0 173 156 672 755.5 0 672 157 295 755.5 0 295 158 283 755.5 0 283 159 171 755.5 0 171 160 205 755.5 0 205 161 220 755.5 0 220 162 218 755.5 0 218 163 244 755.5 0 244 164 388 755.5 0 388 165 280 755.5 0 280 166 268 755.5 0 268 167 242 755.5 0 242 168 336 755.5 0 336 169 504 755.5 0 504 170 159 755.5 0 159 171 148 755.5 0 148 172 245 755.5 0 245 173 320 755.5 0 320 174 82 755.5 0 82 175 116 755.5 0 116 176 135 755.5 0 135 177 170 755.5 0 170 178 260 755.5 0 260 179 266 755.5 0 266 180 222 755.5 0 222 181 442 755.5 0 442 182 363 755.5 0 363 183 409 755.5 0 409 184 359 755.5 0 359 185 332 755.5 0 332 186 338 755.5 0 338 187 172 755.5 0 172 188 191 755.5 0 191 189 238 755.5 0 238 190 292 755.5 0 292 191 291 755.5 0 291 192 259 755.5 0 259 193 282 755.5 0 282 194 169 755.5 0 169 195 168 755.5 0 168 196 465 755.5 0 465 197 590 755.5 0 590 198 233 755.5 0 233 199 114 755.5 0 114 200 60 755.5 0 60 201 180 755.5 0 180 202 391 755.5 0 391 203 327 755.5 0 327 204 254 755.5 0 254 205 486 755.5 0 486 206 514 755.5 0 514 207 226 755.5 0 226 208 397 755.5 0 397 209 360 755.5 0 360 210 314 755.5 0 314 211 385 755.5 0 385 212 310 755.5 0 310 213 403 755.5 0 403 214 385 755.5 0 385 215 351 755.5 0 351 216 524 755.5 0 524 217 470 755.5 0 470 218 465 755.5 0 465 219 356 755.5 0 356 220 298 755.5 0 298 221 646 755.5 0 646 222 1655 755.5 0 1655 223 13955 755.5 0 13955 224 6680 755.5 0 6680 225 7863 755.5 0 7863 226 2091 755.5 0 2091 227 1447 755.5 0 1447 228 310 755.5 0 310 229 278 755.5 0 278 230 314 755.5 0 314 231 211 755.5 0 211 232 203 755.5 0 203 233 426 755.5 0 426 234 659 755.5 0 659 235 383 755.5 0 383 236 560 755.5 0 560 237 519 755.5 0 519 238 319 755.5 0 319 239 244 755.5 0 244 240 205 755.5 0 205 241 212 755.5 0 212 242 277 755.5 0 277 243 272 755.5 0 272 244 232 755.5 0 232 245 403 755.5 0 403 246 320 755.5 0 320 247 223 755.5 0 223 248 142 755.5 0 142 249 108 755.5 0 108 250 278 755.5 0 278 251 417 755.5 0 417 252 293 755.5 0 293 253 178 755.5 0 178 254 256 755.5 0 256 255 334 755.5 0 334 256 423 755.5 0 423 257 245 755.5 0 245 258 203 755.5 0 203 259 216 755.5 0 216 260 327 755.5 0 327 261 397 755.5 0 397 262 460 755.5 0 460 263 274 755.5 0 274 264 337 755.5 0 337 265 277 755.5 0 277 266 493 755.5 0 493 267 417 755.5 0 417 268 265 755.5 0 265 269 191 755.5 0 191 270 406 755.5 0 406 271 406 755.5 0 406 272 420 755.5 0 420 273 444 755.5 0 444 274 456 755.5 0 456 275 192 755.5 0 192 276 227 755.5 0 227 277 346 755.5 0 346 278 360 755.5 0 360 279 360 755.5 0 360 280 216 755.5 0 216 281 180 755.5 0 180 282 359 755.5 0 359 283 360 755.5 0 360 284 313 755.5 0 313 285 355 755.5 0 355 286 246 755.5 0 246 287 286 755.5 0 286 288 239 755.5 0 239 289 236 755.5 0 236 290 365 755.5 0 365 291 434 755.5 0 434 292 488 755.5 0 488 293 322 755.5 0 322 294 207 755.5 0 207 295 438 755.5 0 438 296 936 755.5 0 936 297 706 755.5 0 706 298 412 755.5 0 412 299 677 755.5 0 677 300 505 755.5 0 505 301 383 755.5 0 383 Finished in 6.79 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 198,725 Reads with adapters: 22,426 (11.3%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 22,407 (11.3%) Total basepairs processed: 37,066,089 bp Total written (filtered): 6,104,127 bp (16.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22426 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 3 19 81 0.0 3 0 78 3 20 9538 0.0 4 9247 281 10 21 12721 0.0 4 12515 203 3 22 40 0.0 4 8 29 1 2 23 6 0.0 4 0 0 2 2 2 24 2 0.0 4 0 0 0 2 25 6 0.0 4 0 0 0 1 5 27 3 0.0 4 0 0 0 0 3 31 1 0.0 4 1 54 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 0 2 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 164 2 0.0 4 0 0 0 0 2 168 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.67 s (30 us/read; 2.01 M reads/minute). === Summary === Total reads processed: 22,407 Reads with adapters: 272 (1.2%) Reads written (passing filters): 22,407 (100.0%) Total basepairs processed: 6,104,127 bp Total written (filtered): 6,100,563 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 272 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 0.7% G: 94.5% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 350.1 0 2 4 3 87.5 0 3 6 1 5.5 0 1 9 30 0.1 0 1 29 10 85 0.0 1 3 82 11 148 0.0 1 0 148 260 1 0.0 1 1 264 1 0.0 1 1 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.