This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 96.46 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 206,402 (6.5%) Reads that were too short: 47,341 (1.5%) Reads written (passing filters): 159,061 (5.0%) Total basepairs processed: 961,105,341 bp Total written (filtered): 30,480,894 bp (3.2%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 206402 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 452 779.6 0 452 7 23423 779.6 0 23423 8 17 779.6 0 17 9 5 779.6 0 5 10 14 779.6 0 14 11 15 779.6 0 15 12 8 779.6 0 8 13 12 779.6 0 12 14 7 779.6 0 7 15 10 779.6 0 10 16 7 779.6 0 7 17 9 779.6 0 9 18 11 779.6 0 11 19 8 779.6 0 8 20 11 779.6 0 11 21 5 779.6 0 5 22 13 779.6 0 13 23 14 779.6 0 14 24 11 779.6 0 11 25 5 779.6 0 5 26 14 779.6 0 14 27 21 779.6 0 21 28 17 779.6 0 17 29 74 779.6 0 74 30 304 779.6 0 304 31 128 779.6 0 128 32 10 779.6 0 10 33 10 779.6 0 10 34 10 779.6 0 10 35 14 779.6 0 14 36 20 779.6 0 20 37 5 779.6 0 5 38 11 779.6 0 11 39 13 779.6 0 13 40 17 779.6 0 17 41 15 779.6 0 15 42 13 779.6 0 13 43 14 779.6 0 14 44 20 779.6 0 20 45 11 779.6 0 11 46 20 779.6 0 20 47 8 779.6 0 8 48 10 779.6 0 10 49 9 779.6 0 9 50 17 779.6 0 17 51 11 779.6 0 11 52 11 779.6 0 11 53 8 779.6 0 8 54 3 779.6 0 3 55 12 779.6 0 12 56 21 779.6 0 21 57 33 779.6 0 33 58 23 779.6 0 23 59 17 779.6 0 17 60 13 779.6 0 13 61 32 779.6 0 32 62 14 779.6 0 14 63 14 779.6 0 14 64 18 779.6 0 18 65 20 779.6 0 20 66 28 779.6 0 28 67 36 779.6 0 36 68 57 779.6 0 57 69 75 779.6 0 75 70 1033 779.6 0 1033 71 595 779.6 0 595 72 1874 779.6 0 1874 73 44 779.6 0 44 74 43 779.6 0 43 75 46 779.6 0 46 76 239 779.6 0 239 77 39 779.6 0 39 78 96 779.6 0 96 79 339 779.6 0 339 80 368 779.6 0 368 81 302 779.6 0 302 82 610 779.6 0 610 83 823 779.6 0 823 84 200 779.6 0 200 85 236 779.6 0 236 86 144 779.6 0 144 87 68 779.6 0 68 88 93 779.6 0 93 89 148 779.6 0 148 90 33 779.6 0 33 91 52 779.6 0 52 92 320 779.6 0 320 93 184 779.6 0 184 94 278 779.6 0 278 95 183 779.6 0 183 96 64 779.6 0 64 97 79 779.6 0 79 98 270 779.6 0 270 99 340 779.6 0 340 100 293 779.6 0 293 101 81 779.6 0 81 102 117 779.6 0 117 103 51 779.6 0 51 104 90 779.6 0 90 105 147 779.6 0 147 106 95 779.6 0 95 107 148 779.6 0 148 108 201 779.6 0 201 109 531 779.6 0 531 110 418 779.6 0 418 111 970 779.6 0 970 112 390 779.6 0 390 113 360 779.6 0 360 114 617 779.6 0 617 115 420 779.6 0 420 116 389 779.6 0 389 117 753 779.6 0 753 118 539 779.6 0 539 119 472 779.6 0 472 120 538 779.6 0 538 121 607 779.6 0 607 122 800 779.6 0 800 123 1299 779.6 0 1299 124 16758 779.6 0 16758 125 20204 779.6 0 20204 126 47683 779.6 0 47683 127 1374 779.6 0 1374 128 125 779.6 0 125 129 226 779.6 0 226 130 313 779.6 0 313 131 257 779.6 0 257 132 151 779.6 0 151 133 177 779.6 0 177 134 203 779.6 0 203 135 261 779.6 0 261 136 318 779.6 0 318 137 213 779.6 0 213 138 193 779.6 0 193 139 249 779.6 0 249 140 455 779.6 0 455 141 193 779.6 0 193 142 308 779.6 0 308 143 221 779.6 0 221 144 386 779.6 0 386 145 277 779.6 0 277 146 211 779.6 0 211 147 213 779.6 0 213 148 372 779.6 0 372 149 2704 779.6 0 2704 150 2735 779.6 0 2735 151 6743 779.6 0 6743 152 310 779.6 0 310 153 207 779.6 0 207 154 128 779.6 0 128 155 144 779.6 0 144 156 343 779.6 0 343 157 230 779.6 0 230 158 225 779.6 0 225 159 194 779.6 0 194 160 171 779.6 0 171 161 198 779.6 0 198 162 194 779.6 0 194 163 243 779.6 0 243 164 327 779.6 0 327 165 603 779.6 0 603 166 226 779.6 0 226 167 226 779.6 0 226 168 215 779.6 0 215 169 297 779.6 0 297 170 116 779.6 0 116 171 109 779.6 0 109 172 186 779.6 0 186 173 214 779.6 0 214 174 76 779.6 0 76 175 78 779.6 0 78 176 122 779.6 0 122 177 166 779.6 0 166 178 267 779.6 0 267 179 192 779.6 0 192 180 180 779.6 0 180 181 255 779.6 0 255 182 347 779.6 0 347 183 327 779.6 0 327 184 250 779.6 0 250 185 241 779.6 0 241 186 228 779.6 0 228 187 133 779.6 0 133 188 155 779.6 0 155 189 221 779.6 0 221 190 233 779.6 0 233 191 229 779.6 0 229 192 176 779.6 0 176 193 176 779.6 0 176 194 151 779.6 0 151 195 121 779.6 0 121 196 227 779.6 0 227 197 501 779.6 0 501 198 239 779.6 0 239 199 106 779.6 0 106 200 75 779.6 0 75 201 183 779.6 0 183 202 322 779.6 0 322 203 239 779.6 0 239 204 179 779.6 0 179 205 319 779.6 0 319 206 242 779.6 0 242 207 190 779.6 0 190 208 240 779.6 0 240 209 299 779.6 0 299 210 297 779.6 0 297 211 208 779.6 0 208 212 196 779.6 0 196 213 297 779.6 0 297 214 285 779.6 0 285 215 237 779.6 0 237 216 298 779.6 0 298 217 293 779.6 0 293 218 252 779.6 0 252 219 255 779.6 0 255 220 153 779.6 0 153 221 269 779.6 0 269 222 492 779.6 0 492 223 1604 779.6 0 1604 224 867 779.6 0 867 225 6234 779.6 0 6234 226 4077 779.6 0 4077 227 5177 779.6 0 5177 228 201 779.6 0 201 229 171 779.6 0 171 230 281 779.6 0 281 231 222 779.6 0 222 232 207 779.6 0 207 233 408 779.6 0 408 234 782 779.6 0 782 235 560 779.6 0 560 236 783 779.6 0 783 237 474 779.6 0 474 238 296 779.6 0 296 239 276 779.6 0 276 240 229 779.6 0 229 241 246 779.6 0 246 242 252 779.6 0 252 243 253 779.6 0 253 244 216 779.6 0 216 245 261 779.6 0 261 246 227 779.6 0 227 247 188 779.6 0 188 248 200 779.6 0 200 249 183 779.6 0 183 250 243 779.6 0 243 251 307 779.6 0 307 252 207 779.6 0 207 253 126 779.6 0 126 254 114 779.6 0 114 255 155 779.6 0 155 256 179 779.6 0 179 257 155 779.6 0 155 258 152 779.6 0 152 259 217 779.6 0 217 260 287 779.6 0 287 261 246 779.6 0 246 262 210 779.6 0 210 263 227 779.6 0 227 264 248 779.6 0 248 265 157 779.6 0 157 266 400 779.6 0 400 267 482 779.6 0 482 268 297 779.6 0 297 269 294 779.6 0 294 270 434 779.6 0 434 271 341 779.6 0 341 272 622 779.6 0 622 273 1290 779.6 0 1290 274 619 779.6 0 619 275 202 779.6 0 202 276 265 779.6 0 265 277 309 779.6 0 309 278 267 779.6 0 267 279 258 779.6 0 258 280 173 779.6 0 173 281 185 779.6 0 185 282 228 779.6 0 228 283 212 779.6 0 212 284 283 779.6 0 283 285 249 779.6 0 249 286 156 779.6 0 156 287 293 779.6 0 293 288 376 779.6 0 376 289 356 779.6 0 356 290 380 779.6 0 380 291 388 779.6 0 388 292 382 779.6 0 382 293 303 779.6 0 303 294 276 779.6 0 276 295 371 779.6 0 371 296 592 779.6 0 592 297 504 779.6 0 504 298 393 779.6 0 393 299 670 779.6 0 670 300 575 779.6 0 575 301 249 779.6 0 249 Finished in 6.16 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 159,061 Reads with adapters: 24,374 (15.3%) Reads that were too short: 78 (0.0%) Reads written (passing filters): 24,296 (15.3%) Total basepairs processed: 30,480,894 bp Total written (filtered): 6,628,124 bp (21.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24374 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 8 0.0 3 0 0 4 4 16 92 0.0 3 0 76 12 4 17 3562 0.0 3 3184 340 26 12 18 19915 0.0 3 19279 545 72 19 19 61 0.0 3 6 37 8 10 20 150 0.0 3 0 0 100 50 21 27 0.0 3 0 0 0 27 24 1 0.0 3 0 0 0 1 26 10 0.0 3 0 0 0 10 29 6 0.0 3 0 0 0 6 31 1 0.0 3 0 0 0 1 42 206 0.0 3 0 0 0 206 44 1 0.0 3 0 0 0 1 45 52 0.0 3 0 0 2 50 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 185 0.0 3 0 0 0 185 69 10 0.0 3 0 0 0 10 75 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 102 3 0.0 3 0 0 0 3 103 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 127 8 0.0 3 0 0 0 8 128 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 30 0.0 3 0 0 2 28 145 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 155 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 157 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.00 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 24,296 Reads with adapters: 2,159 (8.9%) Reads written (passing filters): 24,296 (100.0%) Total basepairs processed: 6,628,124 bp Total written (filtered): 6,621,481 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2159 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.9% C: 6.8% G: 49.1% T: 14.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2005 379.6 0 2005 4 146 94.9 0 146 5 6 23.7 0 6 6 1 5.9 0 1 8 1 0.4 0 1