This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.27 s (29 us/read; 2.05 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 64,211 (2.0%) Reads that were too short: 9,575 (0.3%) Reads written (passing filters): 54,636 (1.7%) Total basepairs processed: 961,105,341 bp Total written (filtered): 11,610,911 bp (1.2%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 64211 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4610 779.6 0 4610 7 2464 779.6 0 2464 8 4328 779.6 0 4328 9 5385 779.6 0 5385 10 42 779.6 0 42 11 20 779.6 0 20 12 20 779.6 0 20 13 20 779.6 0 20 14 19 779.6 0 19 15 109 779.6 0 109 16 25 779.6 0 25 17 25 779.6 0 25 18 41 779.6 0 41 19 56 779.6 0 56 20 26 779.6 0 26 21 38 779.6 0 38 22 33 779.6 0 33 23 30 779.6 0 30 24 59 779.6 0 59 25 32 779.6 0 32 26 20 779.6 0 20 27 40 779.6 0 40 28 26 779.6 0 26 29 24 779.6 0 24 30 35 779.6 0 35 31 25 779.6 0 25 32 37 779.6 0 37 33 36 779.6 0 36 34 50 779.6 0 50 35 78 779.6 0 78 36 73 779.6 0 73 37 1297 779.6 0 1297 38 961 779.6 0 961 39 1886 779.6 0 1886 40 214 779.6 0 214 41 16 779.6 0 16 42 26 779.6 0 26 43 21 779.6 0 21 44 19 779.6 0 19 45 25 779.6 0 25 46 38 779.6 0 38 47 14 779.6 0 14 48 21 779.6 0 21 49 24 779.6 0 24 50 14 779.6 0 14 51 19 779.6 0 19 52 20 779.6 0 20 53 10 779.6 0 10 54 25 779.6 0 25 55 21 779.6 0 21 56 12 779.6 0 12 57 13 779.6 0 13 58 20 779.6 0 20 59 11 779.6 0 11 60 17 779.6 0 17 61 17 779.6 0 17 62 26 779.6 0 26 63 14 779.6 0 14 64 14 779.6 0 14 65 17 779.6 0 17 66 14 779.6 0 14 67 20 779.6 0 20 68 26 779.6 0 26 69 21 779.6 0 21 70 21 779.6 0 21 71 40 779.6 0 40 72 30 779.6 0 30 73 14 779.6 0 14 74 23 779.6 0 23 75 56 779.6 0 56 76 22 779.6 0 22 77 19 779.6 0 19 78 19 779.6 0 19 79 51 779.6 0 51 80 15 779.6 0 15 81 19 779.6 0 19 82 25 779.6 0 25 83 19 779.6 0 19 84 28 779.6 0 28 85 17 779.6 0 17 86 19 779.6 0 19 87 21 779.6 0 21 88 17 779.6 0 17 89 19 779.6 0 19 90 40 779.6 0 40 91 274 779.6 0 274 92 443 779.6 0 443 93 183 779.6 0 183 94 53 779.6 0 53 95 34 779.6 0 34 96 16 779.6 0 16 97 22 779.6 0 22 98 14 779.6 0 14 99 17 779.6 0 17 100 23 779.6 0 23 101 38 779.6 0 38 102 14 779.6 0 14 103 23 779.6 0 23 104 31 779.6 0 31 105 20 779.6 0 20 106 22 779.6 0 22 107 20 779.6 0 20 108 16 779.6 0 16 109 17 779.6 0 17 110 27 779.6 0 27 111 32 779.6 0 32 112 45 779.6 0 45 113 118 779.6 0 118 114 73 779.6 0 73 115 47 779.6 0 47 116 53 779.6 0 53 117 20 779.6 0 20 118 41 779.6 0 41 119 20 779.6 0 20 120 22 779.6 0 22 121 19 779.6 0 19 122 19 779.6 0 19 123 29 779.6 0 29 124 32 779.6 0 32 125 28 779.6 0 28 126 37 779.6 0 37 127 66 779.6 0 66 128 135 779.6 0 135 129 391 779.6 0 391 130 159 779.6 0 159 131 771 779.6 0 771 132 140 779.6 0 140 133 73 779.6 0 73 134 58 779.6 0 58 135 113 779.6 0 113 136 159 779.6 0 159 137 145 779.6 0 145 138 134 779.6 0 134 139 173 779.6 0 173 140 273 779.6 0 273 141 544 779.6 0 544 142 14453 779.6 0 14453 143 5753 779.6 0 5753 144 3169 779.6 0 3169 145 34 779.6 0 34 146 23 779.6 0 23 147 12 779.6 0 12 148 24 779.6 0 24 149 70 779.6 0 70 150 332 779.6 0 332 151 173 779.6 0 173 152 10 779.6 0 10 153 17 779.6 0 17 154 16 779.6 0 16 155 14 779.6 0 14 156 16 779.6 0 16 157 19 779.6 0 19 158 15 779.6 0 15 159 11 779.6 0 11 160 14 779.6 0 14 161 15 779.6 0 15 162 19 779.6 0 19 163 14 779.6 0 14 164 18 779.6 0 18 165 17 779.6 0 17 166 24 779.6 0 24 167 14 779.6 0 14 168 25 779.6 0 25 169 22 779.6 0 22 170 45 779.6 0 45 171 44 779.6 0 44 172 17 779.6 0 17 173 20 779.6 0 20 174 16 779.6 0 16 175 27 779.6 0 27 176 18 779.6 0 18 177 39 779.6 0 39 178 115 779.6 0 115 179 923 779.6 0 923 180 15 779.6 0 15 181 18 779.6 0 18 182 23 779.6 0 23 183 14 779.6 0 14 184 9 779.6 0 9 185 19 779.6 0 19 186 14 779.6 0 14 187 14 779.6 0 14 188 16 779.6 0 16 189 15 779.6 0 15 190 29 779.6 0 29 191 63 779.6 0 63 192 73 779.6 0 73 193 10 779.6 0 10 194 20 779.6 0 20 195 15 779.6 0 15 196 23 779.6 0 23 197 16 779.6 0 16 198 10 779.6 0 10 199 16 779.6 0 16 200 19 779.6 0 19 201 29 779.6 0 29 202 25 779.6 0 25 203 105 779.6 0 105 204 30 779.6 0 30 205 66 779.6 0 66 206 23 779.6 0 23 207 23 779.6 0 23 208 25 779.6 0 25 209 31 779.6 0 31 210 35 779.6 0 35 211 414 779.6 0 414 212 65 779.6 0 65 213 85 779.6 0 85 214 572 779.6 0 572 215 373 779.6 0 373 216 540 779.6 0 540 217 41 779.6 0 41 218 40 779.6 0 40 219 34 779.6 0 34 220 43 779.6 0 43 221 40 779.6 0 40 222 18 779.6 0 18 223 14 779.6 0 14 224 43 779.6 0 43 225 90 779.6 0 90 226 58 779.6 0 58 227 86 779.6 0 86 228 111 779.6 0 111 229 228 779.6 0 228 230 85 779.6 0 85 231 134 779.6 0 134 232 91 779.6 0 91 233 64 779.6 0 64 234 128 779.6 0 128 235 1224 779.6 0 1224 236 2808 779.6 0 2808 237 254 779.6 0 254 238 15 779.6 0 15 239 8 779.6 0 8 240 17 779.6 0 17 241 8 779.6 0 8 242 5 779.6 0 5 243 8 779.6 0 8 244 14 779.6 0 14 245 9 779.6 0 9 246 11 779.6 0 11 247 9 779.6 0 9 248 12 779.6 0 12 249 11 779.6 0 11 250 9 779.6 0 9 251 11 779.6 0 11 252 13 779.6 0 13 253 10 779.6 0 10 254 31 779.6 0 31 255 39 779.6 0 39 256 36 779.6 0 36 257 9 779.6 0 9 258 12 779.6 0 12 259 9 779.6 0 9 260 19 779.6 0 19 261 20 779.6 0 20 262 30 779.6 0 30 263 59 779.6 0 59 264 200 779.6 0 200 265 25 779.6 0 25 266 82 779.6 0 82 267 43 779.6 0 43 268 11 779.6 0 11 269 19 779.6 0 19 270 62 779.6 0 62 271 42 779.6 0 42 272 38 779.6 0 38 273 18 779.6 0 18 274 12 779.6 0 12 275 11 779.6 0 11 276 15 779.6 0 15 277 24 779.6 0 24 278 12 779.6 0 12 279 10 779.6 0 10 280 12 779.6 0 12 281 15 779.6 0 15 282 8 779.6 0 8 283 19 779.6 0 19 284 27 779.6 0 27 285 10 779.6 0 10 286 9 779.6 0 9 287 33 779.6 0 33 288 11 779.6 0 11 289 10 779.6 0 10 290 20 779.6 0 20 291 20 779.6 0 20 292 14 779.6 0 14 293 21 779.6 0 21 294 26 779.6 0 26 295 25 779.6 0 25 296 34 779.6 0 34 297 43 779.6 0 43 298 38 779.6 0 38 299 30 779.6 0 30 300 29 779.6 0 29 301 17 779.6 0 17 Finished in 2.25 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 54,636 Reads with adapters: 8,458 (15.5%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 8,444 (15.5%) Total basepairs processed: 11,610,911 bp Total written (filtered): 2,330,580 bp (20.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8458 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 41 0.0 3 0 37 3 1 17 4186 0.0 3 4019 148 13 6 18 4035 0.0 3 3892 123 15 5 19 45 0.0 3 3 8 23 11 20 99 0.0 3 83 6 1 9 21 9 0.0 3 1 0 0 8 36 1 0.0 3 1 42 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 0 2 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 1 1 77 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 1 176 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 214 3 0.0 3 0 0 0 3 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 283 3 0.0 3 0 0 0 3 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 8,444 Reads with adapters: 322 (3.8%) Reads written (passing filters): 8,444 (100.0%) Total basepairs processed: 2,330,580 bp Total written (filtered): 2,329,583 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 322 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.6% C: 57.8% G: 13.7% T: 9.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 297 131.9 0 297 4 19 33.0 0 19 5 6 8.2 0 6