This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 95.63 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 17,924 (0.6%) Reads that were too short: 5,142 (0.2%) Reads written (passing filters): 12,782 (0.4%) Total basepairs processed: 961,105,341 bp Total written (filtered): 3,000,926 bp (0.3%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 17924 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 495 779.6 0 495 7 117 779.6 0 117 8 4397 779.6 0 4397 9 30 779.6 0 30 10 15 779.6 0 15 11 3 779.6 0 3 12 11 779.6 0 11 13 14 779.6 0 14 14 17 779.6 0 17 15 14 779.6 0 14 16 13 779.6 0 13 17 16 779.6 0 16 18 12 779.6 0 12 19 8 779.6 0 8 20 19 779.6 0 19 21 9 779.6 0 9 22 12 779.6 0 12 23 6 779.6 0 6 24 21 779.6 0 21 25 15 779.6 0 15 26 11 779.6 0 11 27 12 779.6 0 12 28 24 779.6 0 24 29 23 779.6 0 23 30 29 779.6 0 29 31 190 779.6 0 190 32 664 779.6 0 664 33 18 779.6 0 18 34 17 779.6 0 17 35 14 779.6 0 14 36 12 779.6 0 12 37 18 779.6 0 18 38 14 779.6 0 14 39 11 779.6 0 11 40 10 779.6 0 10 41 18 779.6 0 18 42 16 779.6 0 16 43 36 779.6 0 36 44 14 779.6 0 14 45 13 779.6 0 13 46 19 779.6 0 19 47 16 779.6 0 16 48 17 779.6 0 17 49 18 779.6 0 18 50 15 779.6 0 15 51 22 779.6 0 22 52 18 779.6 0 18 53 21 779.6 0 21 54 28 779.6 0 28 55 32 779.6 0 32 56 21 779.6 0 21 57 8 779.6 0 8 58 15 779.6 0 15 59 36 779.6 0 36 60 16 779.6 0 16 61 33 779.6 0 33 62 22 779.6 0 22 63 36 779.6 0 36 64 369 779.6 0 369 65 461 779.6 0 461 66 564 779.6 0 564 67 26 779.6 0 26 68 17 779.6 0 17 69 20 779.6 0 20 70 8 779.6 0 8 71 13 779.6 0 13 72 9 779.6 0 9 73 11 779.6 0 11 74 15 779.6 0 15 75 12 779.6 0 12 76 6 779.6 0 6 77 12 779.6 0 12 78 9 779.6 0 9 79 14 779.6 0 14 80 24 779.6 0 24 81 27 779.6 0 27 82 8 779.6 0 8 83 38 779.6 0 38 84 36 779.6 0 36 85 20 779.6 0 20 86 16 779.6 0 16 87 13 779.6 0 13 88 9 779.6 0 9 89 23 779.6 0 23 90 35 779.6 0 35 91 32 779.6 0 32 92 80 779.6 0 80 93 160 779.6 0 160 94 161 779.6 0 161 95 15 779.6 0 15 96 38 779.6 0 38 97 23 779.6 0 23 98 8 779.6 0 8 99 13 779.6 0 13 100 66 779.6 0 66 101 49 779.6 0 49 102 24 779.6 0 24 103 30 779.6 0 30 104 8 779.6 0 8 105 13 779.6 0 13 106 11 779.6 0 11 107 25 779.6 0 25 108 30 779.6 0 30 109 13 779.6 0 13 110 10 779.6 0 10 111 15 779.6 0 15 112 11 779.6 0 11 113 12 779.6 0 12 114 19 779.6 0 19 115 20 779.6 0 20 116 6 779.6 0 6 117 12 779.6 0 12 118 26 779.6 0 26 119 13 779.6 0 13 120 16 779.6 0 16 121 36 779.6 0 36 122 35 779.6 0 35 123 22 779.6 0 22 124 29 779.6 0 29 125 17 779.6 0 17 126 18 779.6 0 18 127 12 779.6 0 12 128 49 779.6 0 49 129 15 779.6 0 15 130 22 779.6 0 22 131 27 779.6 0 27 132 29 779.6 0 29 133 38 779.6 0 38 134 12 779.6 0 12 135 22 779.6 0 22 136 66 779.6 0 66 137 94 779.6 0 94 138 40 779.6 0 40 139 27 779.6 0 27 140 23 779.6 0 23 141 28 779.6 0 28 142 26 779.6 0 26 143 13 779.6 0 13 144 19 779.6 0 19 145 12 779.6 0 12 146 30 779.6 0 30 147 75 779.6 0 75 148 41 779.6 0 41 149 39 779.6 0 39 150 57 779.6 0 57 151 15 779.6 0 15 152 27 779.6 0 27 153 55 779.6 0 55 154 20 779.6 0 20 155 32 779.6 0 32 156 46 779.6 0 46 157 49 779.6 0 49 158 87 779.6 0 87 159 57 779.6 0 57 160 48 779.6 0 48 161 30 779.6 0 30 162 39 779.6 0 39 163 38 779.6 0 38 164 35 779.6 0 35 165 22 779.6 0 22 166 36 779.6 0 36 167 80 779.6 0 80 168 68 779.6 0 68 169 88 779.6 0 88 170 39 779.6 0 39 171 53 779.6 0 53 172 35 779.6 0 35 173 37 779.6 0 37 174 21 779.6 0 21 175 119 779.6 0 119 176 93 779.6 0 93 177 79 779.6 0 79 178 22 779.6 0 22 179 18 779.6 0 18 180 21 779.6 0 21 181 43 779.6 0 43 182 27 779.6 0 27 183 40 779.6 0 40 184 56 779.6 0 56 185 36 779.6 0 36 186 67 779.6 0 67 187 80 779.6 0 80 188 162 779.6 0 162 189 33 779.6 0 33 190 47 779.6 0 47 191 26 779.6 0 26 192 25 779.6 0 25 193 14 779.6 0 14 194 22 779.6 0 22 195 19 779.6 0 19 196 14 779.6 0 14 197 21 779.6 0 21 198 19 779.6 0 19 199 28 779.6 0 28 200 37 779.6 0 37 201 9 779.6 0 9 202 21 779.6 0 21 203 17 779.6 0 17 204 17 779.6 0 17 205 32 779.6 0 32 206 31 779.6 0 31 207 36 779.6 0 36 208 22 779.6 0 22 209 18 779.6 0 18 210 21 779.6 0 21 211 26 779.6 0 26 212 27 779.6 0 27 213 36 779.6 0 36 214 40 779.6 0 40 215 53 779.6 0 53 216 60 779.6 0 60 217 91 779.6 0 91 218 60 779.6 0 60 219 40 779.6 0 40 220 37 779.6 0 37 221 49 779.6 0 49 222 51 779.6 0 51 223 105 779.6 0 105 224 90 779.6 0 90 225 65 779.6 0 65 226 68 779.6 0 68 227 52 779.6 0 52 228 83 779.6 0 83 229 55 779.6 0 55 230 28 779.6 0 28 231 27 779.6 0 27 232 34 779.6 0 34 233 23 779.6 0 23 234 32 779.6 0 32 235 27 779.6 0 27 236 49 779.6 0 49 237 57 779.6 0 57 238 34 779.6 0 34 239 29 779.6 0 29 240 23 779.6 0 23 241 25 779.6 0 25 242 84 779.6 0 84 243 74 779.6 0 74 244 194 779.6 0 194 245 76 779.6 0 76 246 29 779.6 0 29 247 56 779.6 0 56 248 54 779.6 0 54 249 48 779.6 0 48 250 22 779.6 0 22 251 19 779.6 0 19 252 72 779.6 0 72 253 115 779.6 0 115 254 157 779.6 0 157 255 96 779.6 0 96 256 57 779.6 0 57 257 80 779.6 0 80 258 76 779.6 0 76 259 33 779.6 0 33 260 17 779.6 0 17 261 11 779.6 0 11 262 23 779.6 0 23 263 40 779.6 0 40 264 62 779.6 0 62 265 87 779.6 0 87 266 46 779.6 0 46 267 72 779.6 0 72 268 110 779.6 0 110 269 80 779.6 0 80 270 62 779.6 0 62 271 16 779.6 0 16 272 23 779.6 0 23 273 50 779.6 0 50 274 64 779.6 0 64 275 82 779.6 0 82 276 32 779.6 0 32 277 29 779.6 0 29 278 16 779.6 0 16 279 28 779.6 0 28 280 66 779.6 0 66 281 71 779.6 0 71 282 63 779.6 0 63 283 46 779.6 0 46 284 53 779.6 0 53 285 39 779.6 0 39 286 44 779.6 0 44 287 103 779.6 0 103 288 100 779.6 0 100 289 69 779.6 0 69 290 18 779.6 0 18 291 21 779.6 0 21 292 34 779.6 0 34 293 48 779.6 0 48 294 69 779.6 0 69 295 27 779.6 0 27 296 28 779.6 0 28 297 37 779.6 0 37 298 40 779.6 0 40 299 47 779.6 0 47 300 70 779.6 0 70 301 66 779.6 0 66 Finished in 0.64 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 12,782 Reads with adapters: 4,726 (37.0%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 4,726 (37.0%) Total basepairs processed: 3,000,926 bp Total written (filtered): 1,290,030 bp (43.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4726 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 1 0.0 3 0 0 1 19 52 0.0 3 0 51 1 20 4466 0.0 4 4372 88 5 1 21 13 0.0 4 2 11 22 3 0.0 4 0 3 23 187 0.0 4 181 3 1 2 130 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 4,726 Reads with adapters: 206 (4.4%) Reads written (passing filters): 4,726 (100.0%) Total basepairs processed: 1,290,030 bp Total written (filtered): 1,287,123 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 206 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 1.0% G: 96.1% T: 1.0% none/other: 0.5% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 73.8 0 1 10 2 0.0 1 0 2 11 200 0.0 1 0 200 151 1 0.0 1 0 1 260 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.