This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.30 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 145,310 (4.6%) Reads that were too short: 24,442 (0.8%) Reads written (passing filters): 120,868 (3.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 21,879,746 bp (2.3%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 145310 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 114 777.6 0 114 7 312 777.6 0 312 8 13280 777.6 0 13280 9 19 777.6 0 19 10 19 777.6 0 19 11 22 777.6 0 22 12 17 777.6 0 17 13 15 777.6 0 15 14 16 777.6 0 16 15 18 777.6 0 18 16 16 777.6 0 16 17 17 777.6 0 17 18 33 777.6 0 33 19 21 777.6 0 21 20 41 777.6 0 41 21 23 777.6 0 23 22 10 777.6 0 10 23 16 777.6 0 16 24 14 777.6 0 14 25 10 777.6 0 10 26 15 777.6 0 15 27 22 777.6 0 22 28 14 777.6 0 14 29 16 777.6 0 16 30 33 777.6 0 33 31 10 777.6 0 10 32 11 777.6 0 11 33 20 777.6 0 20 34 10 777.6 0 10 35 16 777.6 0 16 36 16 777.6 0 16 37 12 777.6 0 12 38 16 777.6 0 16 39 18 777.6 0 18 40 16 777.6 0 16 41 12 777.6 0 12 42 12 777.6 0 12 43 11 777.6 0 11 44 18 777.6 0 18 45 12 777.6 0 12 46 9 777.6 0 9 47 16 777.6 0 16 48 21 777.6 0 21 49 11 777.6 0 11 50 14 777.6 0 14 51 17 777.6 0 17 52 16 777.6 0 16 53 16 777.6 0 16 54 33 777.6 0 33 55 17 777.6 0 17 56 68 777.6 0 68 57 22 777.6 0 22 58 36 777.6 0 36 59 21 777.6 0 21 60 54 777.6 0 54 61 37 777.6 0 37 62 41 777.6 0 41 63 69 777.6 0 69 64 113 777.6 0 113 65 189 777.6 0 189 66 307 777.6 0 307 67 708 777.6 0 708 68 134 777.6 0 134 69 162 777.6 0 162 70 386 777.6 0 386 71 167 777.6 0 167 72 162 777.6 0 162 73 269 777.6 0 269 74 232 777.6 0 232 75 429 777.6 0 429 76 229 777.6 0 229 77 387 777.6 0 387 78 452 777.6 0 452 79 873 777.6 0 873 80 13916 777.6 0 13916 81 9545 777.6 0 9545 82 19588 777.6 0 19588 83 156 777.6 0 156 84 130 777.6 0 130 85 104 777.6 0 104 86 37 777.6 0 37 87 119 777.6 0 119 88 65 777.6 0 65 89 23 777.6 0 23 90 59 777.6 0 59 91 79 777.6 0 79 92 110 777.6 0 110 93 77 777.6 0 77 94 82 777.6 0 82 95 113 777.6 0 113 96 46 777.6 0 46 97 97 777.6 0 97 98 45 777.6 0 45 99 25 777.6 0 25 100 18 777.6 0 18 101 35 777.6 0 35 102 19 777.6 0 19 103 14 777.6 0 14 104 27 777.6 0 27 105 27 777.6 0 27 106 19 777.6 0 19 107 21 777.6 0 21 108 15 777.6 0 15 109 21 777.6 0 21 110 35 777.6 0 35 111 27 777.6 0 27 112 21 777.6 0 21 113 33 777.6 0 33 114 26 777.6 0 26 115 18 777.6 0 18 116 44 777.6 0 44 117 64 777.6 0 64 118 99 777.6 0 99 119 162 777.6 0 162 120 91 777.6 0 91 121 174 777.6 0 174 122 67 777.6 0 67 123 53 777.6 0 53 124 69 777.6 0 69 125 324 777.6 0 324 126 53 777.6 0 53 127 233 777.6 0 233 128 94 777.6 0 94 129 406 777.6 0 406 130 44 777.6 0 44 131 60 777.6 0 60 132 49 777.6 0 49 133 53 777.6 0 53 134 48 777.6 0 48 135 47 777.6 0 47 136 27 777.6 0 27 137 39 777.6 0 39 138 44 777.6 0 44 139 56 777.6 0 56 140 47 777.6 0 47 141 52 777.6 0 52 142 57 777.6 0 57 143 54 777.6 0 54 144 89 777.6 0 89 145 104 777.6 0 104 146 126 777.6 0 126 147 49 777.6 0 49 148 256 777.6 0 256 149 93 777.6 0 93 150 312 777.6 0 312 151 34 777.6 0 34 152 35 777.6 0 35 153 38 777.6 0 38 154 40 777.6 0 40 155 53 777.6 0 53 156 34 777.6 0 34 157 38 777.6 0 38 158 58 777.6 0 58 159 51 777.6 0 51 160 74 777.6 0 74 161 49 777.6 0 49 162 67 777.6 0 67 163 55 777.6 0 55 164 77 777.6 0 77 165 61 777.6 0 61 166 70 777.6 0 70 167 113 777.6 0 113 168 92 777.6 0 92 169 48 777.6 0 48 170 87 777.6 0 87 171 203 777.6 0 203 172 89 777.6 0 89 173 120 777.6 0 120 174 249 777.6 0 249 175 417 777.6 0 417 176 149 777.6 0 149 177 236 777.6 0 236 178 289 777.6 0 289 179 154 777.6 0 154 180 237 777.6 0 237 181 241 777.6 0 241 182 256 777.6 0 256 183 374 777.6 0 374 184 261 777.6 0 261 185 327 777.6 0 327 186 618 777.6 0 618 187 7242 777.6 0 7242 188 22176 777.6 0 22176 189 4610 777.6 0 4610 190 8132 777.6 0 8132 191 641 777.6 0 641 192 400 777.6 0 400 193 708 777.6 0 708 194 367 777.6 0 367 195 1435 777.6 0 1435 196 629 777.6 0 629 197 33 777.6 0 33 198 51 777.6 0 51 199 81 777.6 0 81 200 195 777.6 0 195 201 283 777.6 0 283 202 53 777.6 0 53 203 60 777.6 0 60 204 113 777.6 0 113 205 107 777.6 0 107 206 981 777.6 0 981 207 506 777.6 0 506 208 1091 777.6 0 1091 209 59 777.6 0 59 210 68 777.6 0 68 211 310 777.6 0 310 212 688 777.6 0 688 213 1058 777.6 0 1058 214 112 777.6 0 112 215 75 777.6 0 75 216 51 777.6 0 51 217 41 777.6 0 41 218 49 777.6 0 49 219 37 777.6 0 37 220 54 777.6 0 54 221 231 777.6 0 231 222 44 777.6 0 44 223 106 777.6 0 106 224 73 777.6 0 73 225 71 777.6 0 71 226 48 777.6 0 48 227 58 777.6 0 58 228 75 777.6 0 75 229 85 777.6 0 85 230 72 777.6 0 72 231 66 777.6 0 66 232 135 777.6 0 135 233 60 777.6 0 60 234 42 777.6 0 42 235 55 777.6 0 55 236 86 777.6 0 86 237 95 777.6 0 95 238 69 777.6 0 69 239 153 777.6 0 153 240 198 777.6 0 198 241 246 777.6 0 246 242 112 777.6 0 112 243 36 777.6 0 36 244 51 777.6 0 51 245 48 777.6 0 48 246 58 777.6 0 58 247 68 777.6 0 68 248 148 777.6 0 148 249 57 777.6 0 57 250 105 777.6 0 105 251 97 777.6 0 97 252 103 777.6 0 103 253 80 777.6 0 80 254 45 777.6 0 45 255 39 777.6 0 39 256 67 777.6 0 67 257 161 777.6 0 161 258 97 777.6 0 97 259 46 777.6 0 46 260 77 777.6 0 77 261 85 777.6 0 85 262 75 777.6 0 75 263 81 777.6 0 81 264 54 777.6 0 54 265 74 777.6 0 74 266 88 777.6 0 88 267 54 777.6 0 54 268 350 777.6 0 350 269 267 777.6 0 267 270 183 777.6 0 183 271 952 777.6 0 952 272 1875 777.6 0 1875 273 1132 777.6 0 1132 274 304 777.6 0 304 275 104 777.6 0 104 276 73 777.6 0 73 277 91 777.6 0 91 278 53 777.6 0 53 279 74 777.6 0 74 280 74 777.6 0 74 281 90 777.6 0 90 282 845 777.6 0 845 283 484 777.6 0 484 284 719 777.6 0 719 285 45 777.6 0 45 286 97 777.6 0 97 287 99 777.6 0 99 288 158 777.6 0 158 289 103 777.6 0 103 290 137 777.6 0 137 291 382 777.6 0 382 292 1158 777.6 0 1158 293 1647 777.6 0 1647 294 1327 777.6 0 1327 295 188 777.6 0 188 296 110 777.6 0 110 297 86 777.6 0 86 298 784 777.6 0 784 299 587 777.6 0 587 300 157 777.6 0 157 301 120 777.6 0 120 Finished in 4.92 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 120,868 Reads with adapters: 231 (0.2%) Reads that were too short: 71 (0.1%) Reads written (passing filters): 160 (0.1%) Total basepairs processed: 21,879,746 bp Total written (filtered): 19,473 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 231 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 20 1 0.0 3 1 23 1 0.0 3 1 24 2 0.0 3 0 0 0 2 32 7 0.0 3 0 0 0 7 42 1 0.0 3 0 0 0 1 43 8 0.0 3 0 0 0 8 44 1 0.0 3 0 0 0 1 46 7 0.0 3 0 0 0 7 51 2 0.0 3 0 0 0 2 52 11 0.0 3 7 3 1 61 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 78 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 86 14 0.0 3 0 0 0 14 87 5 0.0 3 0 0 0 5 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 92 5 0.0 3 0 0 0 5 93 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 7 0.0 3 0 0 0 7 96 3 0.0 3 0 0 1 2 101 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 105 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 109 4 0.0 3 0 0 0 4 111 44 0.0 3 0 0 0 44 114 22 0.0 3 0 0 0 22 126 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 130 5 0.0 3 0 0 0 5 134 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 145 3 0.0 3 0 0 1 2 146 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 212 2 0.0 3 0 0 0 2 213 16 0.0 3 0 0 2 14 214 3 0.0 3 0 0 0 3 216 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 1 265 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (63 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 160 Reads with adapters: 10 (6.2%) Reads written (passing filters): 160 (100.0%) Total basepairs processed: 19,473 bp Total written (filtered): 19,441 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 10 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 30.0% C: 60.0% G: 0.0% T: 10.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8 2.5 0 8 4 2 0.6 0 2