This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 104.43 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 170,353 (5.3%) Reads that were too short: 25,326 (0.8%) Reads written (passing filters): 145,027 (4.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 24,222,752 bp (2.5%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 170353 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 21 777.6 0 21 7 152 777.6 0 152 8 103 777.6 0 103 9 25 777.6 0 25 10 7 777.6 0 7 11 5 777.6 0 5 12 10 777.6 0 10 13 10 777.6 0 10 14 13 777.6 0 13 15 17 777.6 0 17 16 15 777.6 0 15 17 17 777.6 0 17 18 10 777.6 0 10 19 18 777.6 0 18 20 25 777.6 0 25 21 12 777.6 0 12 22 13 777.6 0 13 23 16 777.6 0 16 24 13 777.6 0 13 25 10 777.6 0 10 26 31 777.6 0 31 27 69 777.6 0 69 28 170 777.6 0 170 29 18 777.6 0 18 30 14 777.6 0 14 31 21 777.6 0 21 32 19 777.6 0 19 33 25 777.6 0 25 34 394 777.6 0 394 35 52 777.6 0 52 36 24 777.6 0 24 37 35 777.6 0 35 38 12 777.6 0 12 39 22 777.6 0 22 40 19 777.6 0 19 41 27 777.6 0 27 42 14 777.6 0 14 43 20 777.6 0 20 44 350 777.6 0 350 45 60 777.6 0 60 46 15 777.6 0 15 47 12 777.6 0 12 48 15 777.6 0 15 49 13 777.6 0 13 50 21 777.6 0 21 51 13 777.6 0 13 52 29 777.6 0 29 53 20 777.6 0 20 54 24 777.6 0 24 55 35 777.6 0 35 56 18 777.6 0 18 57 46 777.6 0 46 58 356 777.6 0 356 59 80 777.6 0 80 60 50 777.6 0 50 61 78 777.6 0 78 62 25 777.6 0 25 63 15 777.6 0 15 64 20 777.6 0 20 65 18 777.6 0 18 66 25 777.6 0 25 67 36 777.6 0 36 68 56 777.6 0 56 69 96 777.6 0 96 70 209 777.6 0 209 71 516 777.6 0 516 72 7787 777.6 0 7787 73 2252 777.6 0 2252 74 2722 777.6 0 2722 75 29 777.6 0 29 76 19 777.6 0 19 77 18 777.6 0 18 78 10 777.6 0 10 79 15 777.6 0 15 80 13 777.6 0 13 81 16 777.6 0 16 82 23 777.6 0 23 83 28 777.6 0 28 84 29 777.6 0 29 85 45 777.6 0 45 86 94 777.6 0 94 87 47 777.6 0 47 88 52 777.6 0 52 89 898 777.6 0 898 90 1469 777.6 0 1469 91 78 777.6 0 78 92 576 777.6 0 576 93 67 777.6 0 67 94 44 777.6 0 44 95 79 777.6 0 79 96 197 777.6 0 197 97 237 777.6 0 237 98 15 777.6 0 15 99 29 777.6 0 29 100 63 777.6 0 63 101 53 777.6 0 53 102 35 777.6 0 35 103 54 777.6 0 54 104 27 777.6 0 27 105 19 777.6 0 19 106 43 777.6 0 43 107 31 777.6 0 31 108 29 777.6 0 29 109 171 777.6 0 171 110 142 777.6 0 142 111 48 777.6 0 48 112 98 777.6 0 98 113 155 777.6 0 155 114 119 777.6 0 119 115 39 777.6 0 39 116 46 777.6 0 46 117 69 777.6 0 69 118 78 777.6 0 78 119 201 777.6 0 201 120 54 777.6 0 54 121 82 777.6 0 82 122 80 777.6 0 80 123 110 777.6 0 110 124 96 777.6 0 96 125 83 777.6 0 83 126 160 777.6 0 160 127 271 777.6 0 271 128 312 777.6 0 312 129 654 777.6 0 654 130 1550 777.6 0 1550 131 331 777.6 0 331 132 422 777.6 0 422 133 1294 777.6 0 1294 134 902 777.6 0 902 135 648 777.6 0 648 136 1171 777.6 0 1171 137 821 777.6 0 821 138 1580 777.6 0 1580 139 461 777.6 0 461 140 991 777.6 0 991 141 985 777.6 0 985 142 1383 777.6 0 1383 143 17932 777.6 0 17932 144 12028 777.6 0 12028 145 70586 777.6 0 70586 146 345 777.6 0 345 147 72 777.6 0 72 148 80 777.6 0 80 149 75 777.6 0 75 150 115 777.6 0 115 151 37 777.6 0 37 152 47 777.6 0 47 153 58 777.6 0 58 154 70 777.6 0 70 155 177 777.6 0 177 156 362 777.6 0 362 157 1931 777.6 0 1931 158 149 777.6 0 149 159 82 777.6 0 82 160 39 777.6 0 39 161 79 777.6 0 79 162 99 777.6 0 99 163 353 777.6 0 353 164 221 777.6 0 221 165 1108 777.6 0 1108 166 83 777.6 0 83 167 109 777.6 0 109 168 91 777.6 0 91 169 50 777.6 0 50 170 62 777.6 0 62 171 34 777.6 0 34 172 33 777.6 0 33 173 56 777.6 0 56 174 74 777.6 0 74 175 89 777.6 0 89 176 64 777.6 0 64 177 43 777.6 0 43 178 22 777.6 0 22 179 38 777.6 0 38 180 73 777.6 0 73 181 70 777.6 0 70 182 83 777.6 0 83 183 79 777.6 0 79 184 75 777.6 0 75 185 43 777.6 0 43 186 63 777.6 0 63 187 65 777.6 0 65 188 51 777.6 0 51 189 42 777.6 0 42 190 63 777.6 0 63 191 86 777.6 0 86 192 74 777.6 0 74 193 75 777.6 0 75 194 183 777.6 0 183 195 95 777.6 0 95 196 60 777.6 0 60 197 84 777.6 0 84 198 57 777.6 0 57 199 37 777.6 0 37 200 47 777.6 0 47 201 31 777.6 0 31 202 154 777.6 0 154 203 292 777.6 0 292 204 182 777.6 0 182 205 82 777.6 0 82 206 65 777.6 0 65 207 54 777.6 0 54 208 117 777.6 0 117 209 199 777.6 0 199 210 152 777.6 0 152 211 116 777.6 0 116 212 101 777.6 0 101 213 119 777.6 0 119 214 247 777.6 0 247 215 323 777.6 0 323 216 592 777.6 0 592 217 285 777.6 0 285 218 101 777.6 0 101 219 51 777.6 0 51 220 73 777.6 0 73 221 61 777.6 0 61 222 66 777.6 0 66 223 157 777.6 0 157 224 58 777.6 0 58 225 82 777.6 0 82 226 158 777.6 0 158 227 91 777.6 0 91 228 103 777.6 0 103 229 116 777.6 0 116 230 120 777.6 0 120 231 620 777.6 0 620 232 97 777.6 0 97 233 75 777.6 0 75 234 124 777.6 0 124 235 115 777.6 0 115 236 151 777.6 0 151 237 126 777.6 0 126 238 97 777.6 0 97 239 123 777.6 0 123 240 134 777.6 0 134 241 93 777.6 0 93 242 100 777.6 0 100 243 199 777.6 0 199 244 193 777.6 0 193 245 153 777.6 0 153 246 149 777.6 0 149 247 97 777.6 0 97 248 77 777.6 0 77 249 112 777.6 0 112 250 86 777.6 0 86 251 141 777.6 0 141 252 101 777.6 0 101 253 141 777.6 0 141 254 196 777.6 0 196 255 260 777.6 0 260 256 6235 777.6 0 6235 257 2509 777.6 0 2509 258 3314 777.6 0 3314 259 92 777.6 0 92 260 71 777.6 0 71 261 91 777.6 0 91 262 84 777.6 0 84 263 67 777.6 0 67 264 49 777.6 0 49 265 111 777.6 0 111 266 75 777.6 0 75 267 111 777.6 0 111 268 86 777.6 0 86 269 61 777.6 0 61 270 99 777.6 0 99 271 73 777.6 0 73 272 72 777.6 0 72 273 57 777.6 0 57 274 70 777.6 0 70 275 109 777.6 0 109 276 94 777.6 0 94 277 59 777.6 0 59 278 68 777.6 0 68 279 85 777.6 0 85 280 721 777.6 0 721 281 160 777.6 0 160 282 180 777.6 0 180 283 350 777.6 0 350 284 77 777.6 0 77 285 92 777.6 0 92 286 106 777.6 0 106 287 169 777.6 0 169 288 119 777.6 0 119 289 99 777.6 0 99 290 63 777.6 0 63 291 61 777.6 0 61 292 71 777.6 0 71 293 176 777.6 0 176 294 215 777.6 0 215 295 125 777.6 0 125 296 216 777.6 0 216 297 187 777.6 0 187 298 84 777.6 0 84 299 93 777.6 0 93 300 101 777.6 0 101 301 42 777.6 0 42 Finished in 6.63 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 145,027 Reads with adapters: 16 (0.0%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 7 (0.0%) Total basepairs processed: 24,222,752 bp Total written (filtered): 1,141 bp (0.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 43 2 0.0 4 0 0 0 0 2 52 3 0.0 4 2 0 0 0 1 76 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 146 4 0.0 4 0 0 0 0 4 147 1 0.0 4 0 0 0 0 1 154 1 0.0 4 1 159 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (1429 us/read; 0.04 M reads/minute). === Summary === Total reads processed: 7 Reads with adapters: 0 (0.0%) Reads written (passing filters): 7 (100.0%) Total basepairs processed: 1,141 bp Total written (filtered): 1,141 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.