This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 174.90 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 114,685 (2.2%) Reads that were too short: 21,332 (0.4%) Reads written (passing filters): 93,353 (1.8%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 22,199,409 bp (1.4%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 114685 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1321 1302.2 0 1321 7 38713 1302.2 0 38713 8 114 1302.2 0 114 9 4426 1302.2 0 4426 10 20 1302.2 0 20 11 18 1302.2 0 18 12 18 1302.2 0 18 13 18 1302.2 0 18 14 17 1302.2 0 17 15 12 1302.2 0 12 16 53 1302.2 0 53 17 81 1302.2 0 81 18 73 1302.2 0 73 19 15 1302.2 0 15 20 28 1302.2 0 28 21 17 1302.2 0 17 22 26 1302.2 0 26 23 15 1302.2 0 15 24 87 1302.2 0 87 25 18 1302.2 0 18 26 12 1302.2 0 12 27 20 1302.2 0 20 28 17 1302.2 0 17 29 21 1302.2 0 21 30 21 1302.2 0 21 31 13 1302.2 0 13 32 16 1302.2 0 16 33 30 1302.2 0 30 34 13 1302.2 0 13 35 43 1302.2 0 43 36 18 1302.2 0 18 37 36 1302.2 0 36 38 21 1302.2 0 21 39 26 1302.2 0 26 40 15 1302.2 0 15 41 23 1302.2 0 23 42 30 1302.2 0 30 43 23 1302.2 0 23 44 16 1302.2 0 16 45 26 1302.2 0 26 46 33 1302.2 0 33 47 40 1302.2 0 40 48 1780 1302.2 0 1780 49 19 1302.2 0 19 50 16 1302.2 0 16 51 18 1302.2 0 18 52 49 1302.2 0 49 53 29 1302.2 0 29 54 19 1302.2 0 19 55 36 1302.2 0 36 56 351 1302.2 0 351 57 220 1302.2 0 220 58 356 1302.2 0 356 59 22 1302.2 0 22 60 10 1302.2 0 10 61 7 1302.2 0 7 62 15 1302.2 0 15 63 28 1302.2 0 28 64 7 1302.2 0 7 65 20 1302.2 0 20 66 21 1302.2 0 21 67 18 1302.2 0 18 68 26 1302.2 0 26 69 11 1302.2 0 11 70 22 1302.2 0 22 71 13 1302.2 0 13 72 21 1302.2 0 21 73 55 1302.2 0 55 74 49 1302.2 0 49 75 25 1302.2 0 25 76 47 1302.2 0 47 77 194 1302.2 0 194 78 327 1302.2 0 327 79 372 1302.2 0 372 80 417 1302.2 0 417 81 1961 1302.2 0 1961 82 1093 1302.2 0 1093 83 388 1302.2 0 388 84 808 1302.2 0 808 85 841 1302.2 0 841 86 751 1302.2 0 751 87 217 1302.2 0 217 88 148 1302.2 0 148 89 176 1302.2 0 176 90 165 1302.2 0 165 91 151 1302.2 0 151 92 221 1302.2 0 221 93 373 1302.2 0 373 94 3512 1302.2 0 3512 95 4410 1302.2 0 4410 96 6265 1302.2 0 6265 97 301 1302.2 0 301 98 208 1302.2 0 208 99 115 1302.2 0 115 100 121 1302.2 0 121 101 163 1302.2 0 163 102 44 1302.2 0 44 103 55 1302.2 0 55 104 39 1302.2 0 39 105 24 1302.2 0 24 106 37 1302.2 0 37 107 44 1302.2 0 44 108 38 1302.2 0 38 109 47 1302.2 0 47 110 361 1302.2 0 361 111 150 1302.2 0 150 112 321 1302.2 0 321 113 79 1302.2 0 79 114 73 1302.2 0 73 115 271 1302.2 0 271 116 199 1302.2 0 199 117 195 1302.2 0 195 118 214 1302.2 0 214 119 232 1302.2 0 232 120 167 1302.2 0 167 121 133 1302.2 0 133 122 109 1302.2 0 109 123 142 1302.2 0 142 124 175 1302.2 0 175 125 154 1302.2 0 154 126 223 1302.2 0 223 127 198 1302.2 0 198 128 185 1302.2 0 185 129 125 1302.2 0 125 130 193 1302.2 0 193 131 372 1302.2 0 372 132 545 1302.2 0 545 133 497 1302.2 0 497 134 187 1302.2 0 187 135 132 1302.2 0 132 136 240 1302.2 0 240 137 503 1302.2 0 503 138 462 1302.2 0 462 139 430 1302.2 0 430 140 324 1302.2 0 324 141 249 1302.2 0 249 142 137 1302.2 0 137 143 148 1302.2 0 148 144 171 1302.2 0 171 145 478 1302.2 0 478 146 373 1302.2 0 373 147 148 1302.2 0 148 148 125 1302.2 0 125 149 91 1302.2 0 91 150 108 1302.2 0 108 151 96 1302.2 0 96 152 120 1302.2 0 120 153 82 1302.2 0 82 154 166 1302.2 0 166 155 171 1302.2 0 171 156 155 1302.2 0 155 157 267 1302.2 0 267 158 263 1302.2 0 263 159 261 1302.2 0 261 160 201 1302.2 0 201 161 201 1302.2 0 201 162 150 1302.2 0 150 163 177 1302.2 0 177 164 185 1302.2 0 185 165 261 1302.2 0 261 166 278 1302.2 0 278 167 238 1302.2 0 238 168 214 1302.2 0 214 169 170 1302.2 0 170 170 263 1302.2 0 263 171 138 1302.2 0 138 172 111 1302.2 0 111 173 84 1302.2 0 84 174 204 1302.2 0 204 175 161 1302.2 0 161 176 187 1302.2 0 187 177 107 1302.2 0 107 178 85 1302.2 0 85 179 154 1302.2 0 154 180 314 1302.2 0 314 181 386 1302.2 0 386 182 256 1302.2 0 256 183 196 1302.2 0 196 184 224 1302.2 0 224 185 192 1302.2 0 192 186 230 1302.2 0 230 187 291 1302.2 0 291 188 133 1302.2 0 133 189 52 1302.2 0 52 190 133 1302.2 0 133 191 266 1302.2 0 266 192 536 1302.2 0 536 193 347 1302.2 0 347 194 1046 1302.2 0 1046 195 191 1302.2 0 191 196 109 1302.2 0 109 197 108 1302.2 0 108 198 181 1302.2 0 181 199 219 1302.2 0 219 200 179 1302.2 0 179 201 56 1302.2 0 56 202 57 1302.2 0 57 203 200 1302.2 0 200 204 446 1302.2 0 446 205 549 1302.2 0 549 206 446 1302.2 0 446 207 324 1302.2 0 324 208 134 1302.2 0 134 209 119 1302.2 0 119 210 183 1302.2 0 183 211 338 1302.2 0 338 212 324 1302.2 0 324 213 348 1302.2 0 348 214 289 1302.2 0 289 215 234 1302.2 0 234 216 215 1302.2 0 215 217 238 1302.2 0 238 218 375 1302.2 0 375 219 314 1302.2 0 314 220 243 1302.2 0 243 221 116 1302.2 0 116 222 84 1302.2 0 84 223 195 1302.2 0 195 224 267 1302.2 0 267 225 238 1302.2 0 238 226 241 1302.2 0 241 227 298 1302.2 0 298 228 294 1302.2 0 294 229 246 1302.2 0 246 230 147 1302.2 0 147 231 160 1302.2 0 160 232 152 1302.2 0 152 233 91 1302.2 0 91 234 173 1302.2 0 173 235 265 1302.2 0 265 236 280 1302.2 0 280 237 164 1302.2 0 164 238 224 1302.2 0 224 239 239 1302.2 0 239 240 192 1302.2 0 192 241 82 1302.2 0 82 242 87 1302.2 0 87 243 143 1302.2 0 143 244 243 1302.2 0 243 245 233 1302.2 0 233 246 154 1302.2 0 154 247 163 1302.2 0 163 248 104 1302.2 0 104 249 108 1302.2 0 108 250 91 1302.2 0 91 251 93 1302.2 0 93 252 130 1302.2 0 130 253 177 1302.2 0 177 254 158 1302.2 0 158 255 136 1302.2 0 136 256 144 1302.2 0 144 257 181 1302.2 0 181 258 131 1302.2 0 131 259 131 1302.2 0 131 260 83 1302.2 0 83 261 113 1302.2 0 113 262 221 1302.2 0 221 263 157 1302.2 0 157 264 150 1302.2 0 150 265 230 1302.2 0 230 266 264 1302.2 0 264 267 313 1302.2 0 313 268 332 1302.2 0 332 269 115 1302.2 0 115 270 67 1302.2 0 67 271 187 1302.2 0 187 272 533 1302.2 0 533 273 281 1302.2 0 281 274 199 1302.2 0 199 275 157 1302.2 0 157 276 151 1302.2 0 151 277 91 1302.2 0 91 278 161 1302.2 0 161 279 296 1302.2 0 296 280 194 1302.2 0 194 281 106 1302.2 0 106 282 96 1302.2 0 96 283 105 1302.2 0 105 284 142 1302.2 0 142 285 176 1302.2 0 176 286 236 1302.2 0 236 287 257 1302.2 0 257 288 329 1302.2 0 329 289 260 1302.2 0 260 290 251 1302.2 0 251 291 308 1302.2 0 308 292 338 1302.2 0 338 293 268 1302.2 0 268 294 197 1302.2 0 197 295 158 1302.2 0 158 296 109 1302.2 0 109 297 337 1302.2 0 337 298 564 1302.2 0 564 299 252 1302.2 0 252 300 110 1302.2 0 110 301 307 1302.2 0 307 Finished in 4.59 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 93,353 Reads with adapters: 4,955 (5.3%) Reads that were too short: 59 (0.1%) Reads written (passing filters): 4,896 (5.2%) Total basepairs processed: 22,199,409 bp Total written (filtered): 1,340,366 bp (6.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4955 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 0 3 16 35 0.0 3 0 23 1 11 17 4308 0.0 3 4201 91 15 1 18 27 0.0 3 1 7 17 2 19 28 0.0 3 3 6 3 16 20 453 0.0 3 436 16 1 21 1 0.0 3 0 0 1 22 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 9 0.0 3 0 0 0 9 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 270 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 6 0.0 3 0 0 0 6 282 9 0.0 3 0 0 0 9 283 8 0.0 3 0 0 0 8 288 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 4,896 Reads with adapters: 372 (7.6%) Reads written (passing filters): 4,896 (100.0%) Total basepairs processed: 1,340,366 bp Total written (filtered): 1,339,181 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 372 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.8% C: 13.7% G: 12.4% T: 13.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 309 76.5 0 309 4 60 19.1 0 60 5 1 4.8 0 1 6 1 1.2 0 1 7 1 0.3 0 1