This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 171.63 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 289,973 (5.4%) Reads that were too short: 126,965 (2.4%) Reads written (passing filters): 163,008 (3.1%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 29,788,171 bp (1.9%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 289973 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 858 1302.2 0 858 7 3855 1302.2 0 3855 8 451 1302.2 0 451 9 26555 1302.2 0 26555 10 21 1302.2 0 21 11 15 1302.2 0 15 12 8 1302.2 0 8 13 15 1302.2 0 15 14 4 1302.2 0 4 15 6 1302.2 0 6 16 8 1302.2 0 8 17 8 1302.2 0 8 18 6 1302.2 0 6 19 10 1302.2 0 10 20 12 1302.2 0 12 21 6 1302.2 0 6 22 9 1302.2 0 9 23 10 1302.2 0 10 24 6 1302.2 0 6 25 11 1302.2 0 11 26 16 1302.2 0 16 27 13 1302.2 0 13 28 13 1302.2 0 13 29 8 1302.2 0 8 30 11 1302.2 0 11 31 10 1302.2 0 10 32 11 1302.2 0 11 33 12 1302.2 0 12 34 8 1302.2 0 8 35 7 1302.2 0 7 36 6 1302.2 0 6 37 11 1302.2 0 11 38 15 1302.2 0 15 39 11 1302.2 0 11 40 12 1302.2 0 12 41 9 1302.2 0 9 42 10 1302.2 0 10 43 12 1302.2 0 12 44 26 1302.2 0 26 45 2 1302.2 0 2 46 10 1302.2 0 10 47 20 1302.2 0 20 48 20 1302.2 0 20 49 11 1302.2 0 11 50 19 1302.2 0 19 51 12 1302.2 0 12 52 6 1302.2 0 6 53 10 1302.2 0 10 54 11 1302.2 0 11 55 6 1302.2 0 6 56 32 1302.2 0 32 57 23 1302.2 0 23 58 7 1302.2 0 7 59 31 1302.2 0 31 60 10 1302.2 0 10 61 10 1302.2 0 10 62 15 1302.2 0 15 63 17 1302.2 0 17 64 13 1302.2 0 13 65 14 1302.2 0 14 66 10 1302.2 0 10 67 16 1302.2 0 16 68 19 1302.2 0 19 69 15 1302.2 0 15 70 12 1302.2 0 12 71 14 1302.2 0 14 72 22 1302.2 0 22 73 37 1302.2 0 37 74 9 1302.2 0 9 75 16 1302.2 0 16 76 21 1302.2 0 21 77 10 1302.2 0 10 78 26 1302.2 0 26 79 173 1302.2 0 173 80 101 1302.2 0 101 81 214 1302.2 0 214 82 251 1302.2 0 251 83 448 1302.2 0 448 84 200 1302.2 0 200 85 234 1302.2 0 234 86 159 1302.2 0 159 87 747 1302.2 0 747 88 511 1302.2 0 511 89 1697 1302.2 0 1697 90 388 1302.2 0 388 91 120 1302.2 0 120 92 424 1302.2 0 424 93 404 1302.2 0 404 94 411 1302.2 0 411 95 123 1302.2 0 123 96 61 1302.2 0 61 97 72 1302.2 0 72 98 171 1302.2 0 171 99 706 1302.2 0 706 100 1359 1302.2 0 1359 101 257 1302.2 0 257 102 569 1302.2 0 569 103 960 1302.2 0 960 104 1319 1302.2 0 1319 105 1980 1302.2 0 1980 106 778 1302.2 0 778 107 270 1302.2 0 270 108 331 1302.2 0 331 109 718 1302.2 0 718 110 112 1302.2 0 112 111 175 1302.2 0 175 112 269 1302.2 0 269 113 247 1302.2 0 247 114 253 1302.2 0 253 115 182 1302.2 0 182 116 352 1302.2 0 352 117 444 1302.2 0 444 118 425 1302.2 0 425 119 431 1302.2 0 431 120 1062 1302.2 0 1062 121 604 1302.2 0 604 122 786 1302.2 0 786 123 318 1302.2 0 318 124 330 1302.2 0 330 125 325 1302.2 0 325 126 405 1302.2 0 405 127 163 1302.2 0 163 128 208 1302.2 0 208 129 427 1302.2 0 427 130 554 1302.2 0 554 131 614 1302.2 0 614 132 397 1302.2 0 397 133 728 1302.2 0 728 134 916 1302.2 0 916 135 1149 1302.2 0 1149 136 506 1302.2 0 506 137 608 1302.2 0 608 138 733 1302.2 0 733 139 849 1302.2 0 849 140 6605 1302.2 0 6605 141 2244 1302.2 0 2244 142 2375 1302.2 0 2375 143 546 1302.2 0 546 144 1195 1302.2 0 1195 145 1689 1302.2 0 1689 146 18353 1302.2 0 18353 147 5652 1302.2 0 5652 148 9073 1302.2 0 9073 149 707 1302.2 0 707 150 5786 1302.2 0 5786 151 2069 1302.2 0 2069 152 4297 1302.2 0 4297 153 455 1302.2 0 455 154 374 1302.2 0 374 155 499 1302.2 0 499 156 5476 1302.2 0 5476 157 3271 1302.2 0 3271 158 2443 1302.2 0 2443 159 723 1302.2 0 723 160 1111 1302.2 0 1111 161 1035 1302.2 0 1035 162 1074 1302.2 0 1074 163 518 1302.2 0 518 164 806 1302.2 0 806 165 607 1302.2 0 607 166 907 1302.2 0 907 167 592 1302.2 0 592 168 1028 1302.2 0 1028 169 784 1302.2 0 784 170 470 1302.2 0 470 171 254 1302.2 0 254 172 234 1302.2 0 234 173 563 1302.2 0 563 174 157 1302.2 0 157 175 231 1302.2 0 231 176 258 1302.2 0 258 177 285 1302.2 0 285 178 367 1302.2 0 367 179 700 1302.2 0 700 180 737 1302.2 0 737 181 1151 1302.2 0 1151 182 2011 1302.2 0 2011 183 1353 1302.2 0 1353 184 1280 1302.2 0 1280 185 890 1302.2 0 890 186 932 1302.2 0 932 187 257 1302.2 0 257 188 406 1302.2 0 406 189 470 1302.2 0 470 190 557 1302.2 0 557 191 702 1302.2 0 702 192 268 1302.2 0 268 193 260 1302.2 0 260 194 209 1302.2 0 209 195 278 1302.2 0 278 196 283 1302.2 0 283 197 875 1302.2 0 875 198 283 1302.2 0 283 199 120 1302.2 0 120 200 128 1302.2 0 128 201 396 1302.2 0 396 202 1115 1302.2 0 1115 203 1219 1302.2 0 1219 204 864 1302.2 0 864 205 809 1302.2 0 809 206 797 1302.2 0 797 207 1547 1302.2 0 1547 208 2526 1302.2 0 2526 209 1783 1302.2 0 1783 210 981 1302.2 0 981 211 804 1302.2 0 804 212 1727 1302.2 0 1727 213 1303 1302.2 0 1303 214 996 1302.2 0 996 215 1015 1302.2 0 1015 216 1254 1302.2 0 1254 217 1162 1302.2 0 1162 218 643 1302.2 0 643 219 549 1302.2 0 549 220 1738 1302.2 0 1738 221 3036 1302.2 0 3036 222 4095 1302.2 0 4095 223 2086 1302.2 0 2086 224 1290 1302.2 0 1290 225 1894 1302.2 0 1894 226 1331 1302.2 0 1331 227 935 1302.2 0 935 228 934 1302.2 0 934 229 490 1302.2 0 490 230 457 1302.2 0 457 231 407 1302.2 0 407 232 905 1302.2 0 905 233 871 1302.2 0 871 234 1465 1302.2 0 1465 235 1543 1302.2 0 1543 236 715 1302.2 0 715 237 477 1302.2 0 477 238 282 1302.2 0 282 239 185 1302.2 0 185 240 195 1302.2 0 195 241 649 1302.2 0 649 242 663 1302.2 0 663 243 1608 1302.2 0 1608 244 983 1302.2 0 983 245 840 1302.2 0 840 246 470 1302.2 0 470 247 399 1302.2 0 399 248 224 1302.2 0 224 249 212 1302.2 0 212 250 486 1302.2 0 486 251 1329 1302.2 0 1329 252 2000 1302.2 0 2000 253 776 1302.2 0 776 254 1439 1302.2 0 1439 255 4383 1302.2 0 4383 256 15719 1302.2 0 15719 257 4743 1302.2 0 4743 258 3259 1302.2 0 3259 259 626 1302.2 0 626 260 925 1302.2 0 925 261 1102 1302.2 0 1102 262 1743 1302.2 0 1743 263 1606 1302.2 0 1606 264 1336 1302.2 0 1336 265 926 1302.2 0 926 266 1383 1302.2 0 1383 267 951 1302.2 0 951 268 474 1302.2 0 474 269 141 1302.2 0 141 270 618 1302.2 0 618 271 946 1302.2 0 946 272 626 1302.2 0 626 273 582 1302.2 0 582 274 558 1302.2 0 558 275 500 1302.2 0 500 276 392 1302.2 0 392 277 514 1302.2 0 514 278 934 1302.2 0 934 279 1364 1302.2 0 1364 280 2142 1302.2 0 2142 281 1705 1302.2 0 1705 282 2284 1302.2 0 2284 283 3915 1302.2 0 3915 284 2072 1302.2 0 2072 285 1765 1302.2 0 1765 286 547 1302.2 0 547 287 431 1302.2 0 431 288 191 1302.2 0 191 289 217 1302.2 0 217 290 680 1302.2 0 680 291 680 1302.2 0 680 292 898 1302.2 0 898 293 991 1302.2 0 991 294 356 1302.2 0 356 295 260 1302.2 0 260 296 361 1302.2 0 361 297 326 1302.2 0 326 298 785 1302.2 0 785 299 781 1302.2 0 781 300 534 1302.2 0 534 301 190 1302.2 0 190 Finished in 7.58 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 163,008 Reads with adapters: 4,430 (2.7%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 4,419 (2.7%) Total basepairs processed: 29,788,171 bp Total written (filtered): 1,200,392 bp (4.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4430 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 3 0 1 19 32 0.0 3 0 32 20 4224 0.0 4 4150 71 2 0 1 21 35 0.0 4 27 7 1 22 2 0.0 4 0 0 1 1 23 26 0.0 4 24 0 0 1 1 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 68 0.0 4 0 0 0 0 68 153 19 0.0 4 0 0 0 0 19 168 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 4,419 Reads with adapters: 3,483 (78.8%) Reads written (passing filters): 4,419 (100.0%) Total basepairs processed: 1,200,392 bp Total written (filtered): 251,718 bp (21.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3483 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.1% T: 74.3% none/other: 25.3% Overview of removed sequences length count expect max.err error counts 3 1 69.0 0 1 263 27 0.0 1 27 264 3 0.0 1 3 266 3 0.0 1 3 267 434 0.0 1 404 30 268 53 0.0 1 52 1 271 15 0.0 1 15 272 6 0.0 1 6 273 1757 0.0 1 1683 74 274 1062 0.0 1 1013 49 275 120 0.0 1 116 4 276 2 0.0 1 2