This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 174.67 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 631,114 (11.8%) Reads that were too short: 240,448 (4.5%) Reads written (passing filters): 390,666 (7.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 69,361,403 bp (4.3%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 631114 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 115 1302.2 0 115 7 2963 1302.2 0 2963 8 3111 1302.2 0 3111 9 19 1302.2 0 19 10 79 1302.2 0 79 11 91 1302.2 0 91 12 91 1302.2 0 91 13 49 1302.2 0 49 14 140 1302.2 0 140 15 8 1302.2 0 8 16 5 1302.2 0 5 17 1 1302.2 0 1 18 19 1302.2 0 19 19 2 1302.2 0 2 20 12 1302.2 0 12 21 3 1302.2 0 3 22 7 1302.2 0 7 23 21 1302.2 0 21 24 19 1302.2 0 19 25 12 1302.2 0 12 26 11 1302.2 0 11 27 9 1302.2 0 9 28 17 1302.2 0 17 29 15 1302.2 0 15 30 15 1302.2 0 15 31 8 1302.2 0 8 32 24 1302.2 0 24 33 8 1302.2 0 8 34 3 1302.2 0 3 35 17 1302.2 0 17 36 3 1302.2 0 3 37 44 1302.2 0 44 38 19 1302.2 0 19 39 31 1302.2 0 31 40 16 1302.2 0 16 41 9 1302.2 0 9 42 23 1302.2 0 23 43 17 1302.2 0 17 44 15 1302.2 0 15 45 14 1302.2 0 14 46 15 1302.2 0 15 47 18 1302.2 0 18 48 12 1302.2 0 12 49 24 1302.2 0 24 50 12 1302.2 0 12 51 20 1302.2 0 20 52 110 1302.2 0 110 53 21 1302.2 0 21 54 52 1302.2 0 52 55 46 1302.2 0 46 56 15 1302.2 0 15 57 119 1302.2 0 119 58 5 1302.2 0 5 59 31 1302.2 0 31 60 23 1302.2 0 23 61 52 1302.2 0 52 62 212 1302.2 0 212 63 39 1302.2 0 39 64 123 1302.2 0 123 65 67 1302.2 0 67 66 139 1302.2 0 139 67 124 1302.2 0 124 68 45 1302.2 0 45 69 56 1302.2 0 56 70 44 1302.2 0 44 71 88 1302.2 0 88 72 336 1302.2 0 336 73 1236 1302.2 0 1236 74 549 1302.2 0 549 75 1675 1302.2 0 1675 76 1115 1302.2 0 1115 77 3149 1302.2 0 3149 78 4608 1302.2 0 4608 79 55723 1302.2 0 55723 80 6960 1302.2 0 6960 81 40076 1302.2 0 40076 82 2093 1302.2 0 2093 83 2718 1302.2 0 2718 84 4550 1302.2 0 4550 85 3330 1302.2 0 3330 86 4020 1302.2 0 4020 87 4230 1302.2 0 4230 88 2964 1302.2 0 2964 89 1082 1302.2 0 1082 90 873 1302.2 0 873 91 4402 1302.2 0 4402 92 779 1302.2 0 779 93 3293 1302.2 0 3293 94 1844 1302.2 0 1844 95 1240 1302.2 0 1240 96 815 1302.2 0 815 97 933 1302.2 0 933 98 470 1302.2 0 470 99 773 1302.2 0 773 100 520 1302.2 0 520 101 1500 1302.2 0 1500 102 638 1302.2 0 638 103 894 1302.2 0 894 104 401 1302.2 0 401 105 492 1302.2 0 492 106 309 1302.2 0 309 107 350 1302.2 0 350 108 411 1302.2 0 411 109 404 1302.2 0 404 110 506 1302.2 0 506 111 1179 1302.2 0 1179 112 955 1302.2 0 955 113 1069 1302.2 0 1069 114 873 1302.2 0 873 115 1596 1302.2 0 1596 116 1819 1302.2 0 1819 117 6356 1302.2 0 6356 118 2913 1302.2 0 2913 119 3879 1302.2 0 3879 120 2098 1302.2 0 2098 121 1641 1302.2 0 1641 122 2093 1302.2 0 2093 123 1655 1302.2 0 1655 124 1976 1302.2 0 1976 125 2060 1302.2 0 2060 126 1818 1302.2 0 1818 127 1627 1302.2 0 1627 128 1653 1302.2 0 1653 129 1840 1302.2 0 1840 130 3560 1302.2 0 3560 131 2242 1302.2 0 2242 132 4572 1302.2 0 4572 133 1882 1302.2 0 1882 134 2370 1302.2 0 2370 135 1927 1302.2 0 1927 136 1991 1302.2 0 1991 137 2242 1302.2 0 2242 138 2255 1302.2 0 2255 139 2220 1302.2 0 2220 140 1650 1302.2 0 1650 141 2083 1302.2 0 2083 142 2113 1302.2 0 2113 143 1915 1302.2 0 1915 144 1879 1302.2 0 1879 145 2169 1302.2 0 2169 146 3282 1302.2 0 3282 147 2171 1302.2 0 2171 148 2504 1302.2 0 2504 149 1673 1302.2 0 1673 150 2455 1302.2 0 2455 151 2896 1302.2 0 2896 152 3294 1302.2 0 3294 153 2627 1302.2 0 2627 154 2389 1302.2 0 2389 155 2567 1302.2 0 2567 156 3108 1302.2 0 3108 157 2983 1302.2 0 2983 158 2945 1302.2 0 2945 159 2997 1302.2 0 2997 160 2398 1302.2 0 2398 161 2680 1302.2 0 2680 162 2507 1302.2 0 2507 163 2495 1302.2 0 2495 164 2092 1302.2 0 2092 165 2064 1302.2 0 2064 166 2061 1302.2 0 2061 167 2503 1302.2 0 2503 168 2978 1302.2 0 2978 169 2057 1302.2 0 2057 170 3411 1302.2 0 3411 171 2076 1302.2 0 2076 172 3602 1302.2 0 3602 173 1539 1302.2 0 1539 174 2934 1302.2 0 2934 175 1696 1302.2 0 1696 176 2545 1302.2 0 2545 177 2069 1302.2 0 2069 178 2875 1302.2 0 2875 179 3015 1302.2 0 3015 180 2658 1302.2 0 2658 181 2955 1302.2 0 2955 182 2465 1302.2 0 2465 183 2460 1302.2 0 2460 184 2548 1302.2 0 2548 185 2379 1302.2 0 2379 186 2793 1302.2 0 2793 187 2547 1302.2 0 2547 188 3973 1302.2 0 3973 189 2209 1302.2 0 2209 190 2424 1302.2 0 2424 191 2060 1302.2 0 2060 192 2594 1302.2 0 2594 193 1770 1302.2 0 1770 194 2517 1302.2 0 2517 195 2143 1302.2 0 2143 196 2474 1302.2 0 2474 197 2075 1302.2 0 2075 198 3050 1302.2 0 3050 199 3080 1302.2 0 3080 200 2885 1302.2 0 2885 201 2091 1302.2 0 2091 202 2206 1302.2 0 2206 203 1980 1302.2 0 1980 204 2554 1302.2 0 2554 205 2108 1302.2 0 2108 206 2406 1302.2 0 2406 207 2644 1302.2 0 2644 208 2139 1302.2 0 2139 209 1562 1302.2 0 1562 210 2060 1302.2 0 2060 211 2194 1302.2 0 2194 212 2569 1302.2 0 2569 213 3368 1302.2 0 3368 214 3243 1302.2 0 3243 215 3060 1302.2 0 3060 216 3140 1302.2 0 3140 217 2739 1302.2 0 2739 218 2604 1302.2 0 2604 219 2453 1302.2 0 2453 220 2763 1302.2 0 2763 221 3536 1302.2 0 3536 222 3426 1302.2 0 3426 223 2521 1302.2 0 2521 224 2685 1302.2 0 2685 225 2136 1302.2 0 2136 226 1867 1302.2 0 1867 227 1843 1302.2 0 1843 228 2315 1302.2 0 2315 229 1672 1302.2 0 1672 230 1740 1302.2 0 1740 231 1929 1302.2 0 1929 232 2971 1302.2 0 2971 233 2227 1302.2 0 2227 234 2621 1302.2 0 2621 235 2291 1302.2 0 2291 236 2559 1302.2 0 2559 237 1823 1302.2 0 1823 238 2943 1302.2 0 2943 239 2742 1302.2 0 2742 240 3467 1302.2 0 3467 241 3698 1302.2 0 3698 242 3915 1302.2 0 3915 243 3412 1302.2 0 3412 244 2417 1302.2 0 2417 245 2075 1302.2 0 2075 246 1323 1302.2 0 1323 247 1864 1302.2 0 1864 248 2223 1302.2 0 2223 249 3117 1302.2 0 3117 250 3090 1302.2 0 3090 251 4251 1302.2 0 4251 252 3833 1302.2 0 3833 253 2073 1302.2 0 2073 254 1542 1302.2 0 1542 255 1022 1302.2 0 1022 256 929 1302.2 0 929 257 1072 1302.2 0 1072 258 1357 1302.2 0 1357 259 1555 1302.2 0 1555 260 1879 1302.2 0 1879 261 2027 1302.2 0 2027 262 2499 1302.2 0 2499 263 2558 1302.2 0 2558 264 2484 1302.2 0 2484 265 1691 1302.2 0 1691 266 1674 1302.2 0 1674 267 1663 1302.2 0 1663 268 1758 1302.2 0 1758 269 2486 1302.2 0 2486 270 2442 1302.2 0 2442 271 3366 1302.2 0 3366 272 2740 1302.2 0 2740 273 2408 1302.2 0 2408 274 1894 1302.2 0 1894 275 2592 1302.2 0 2592 276 3202 1302.2 0 3202 277 3161 1302.2 0 3161 278 2977 1302.2 0 2977 279 2289 1302.2 0 2289 280 2488 1302.2 0 2488 281 2193 1302.2 0 2193 282 2271 1302.2 0 2271 283 2289 1302.2 0 2289 284 1984 1302.2 0 1984 285 1542 1302.2 0 1542 286 1551 1302.2 0 1551 287 1846 1302.2 0 1846 288 2389 1302.2 0 2389 289 2651 1302.2 0 2651 290 4175 1302.2 0 4175 291 3623 1302.2 0 3623 292 3197 1302.2 0 3197 293 2230 1302.2 0 2230 294 2411 1302.2 0 2411 295 2390 1302.2 0 2390 296 3030 1302.2 0 3030 297 2371 1302.2 0 2371 298 2331 1302.2 0 2331 299 2011 1302.2 0 2011 300 1414 1302.2 0 1414 301 397 1302.2 0 397 Finished in 15.15 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 390,666 Reads with adapters: 2,288 (0.6%) Reads that were too short: 212 (0.1%) Reads written (passing filters): 2,076 (0.5%) Total basepairs processed: 69,361,403 bp Total written (filtered): 558,160 bp (0.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2288 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 21 0.0 3 0 18 1 2 17 1917 0.0 3 1813 85 14 5 18 15 0.0 3 0 7 6 2 19 12 0.0 3 2 0 2 8 20 3 0.0 3 0 0 0 3 22 2 0.0 3 0 0 1 1 23 7 0.0 3 0 0 0 7 25 1 0.0 3 0 0 0 1 26 3 0.0 3 0 0 0 3 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 40 3 0.0 3 0 0 0 3 41 18 0.0 3 0 0 0 18 42 2 0.0 3 0 0 0 2 44 11 0.0 3 0 0 0 11 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 55 6 0.0 3 0 0 0 6 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 1 2 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 3 0.0 3 0 0 0 3 113 2 0.0 3 0 0 0 2 115 3 0.0 3 0 0 0 3 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 20 0.0 3 0 0 0 20 150 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 207 5 0.0 3 0 0 0 5 208 1 0.0 3 0 0 0 1 209 17 0.0 3 0 0 0 17 210 72 0.0 3 0 0 1 71 211 29 0.0 3 0 0 0 29 216 2 0.0 3 0 0 0 2 222 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 2,076 Reads with adapters: 53 (2.6%) Reads written (passing filters): 2,076 (100.0%) Total basepairs processed: 558,160 bp Total written (filtered): 557,999 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 53 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.5% C: 28.3% G: 13.2% T: 17.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 51 32.4 0 51 4 2 8.1 0 2