This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 173.18 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 416,450 (7.8%) Reads that were too short: 209,576 (3.9%) Reads written (passing filters): 206,874 (3.9%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 34,610,404 bp (2.2%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 416450 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 87 1302.2 0 87 7 1353 1302.2 0 1353 8 8625 1302.2 0 8625 9 14 1302.2 0 14 10 11 1302.2 0 11 11 7 1302.2 0 7 12 12 1302.2 0 12 13 11 1302.2 0 11 14 6 1302.2 0 6 15 8 1302.2 0 8 16 6 1302.2 0 6 17 7 1302.2 0 7 18 12 1302.2 0 12 19 9 1302.2 0 9 20 12 1302.2 0 12 21 4 1302.2 0 4 22 10 1302.2 0 10 23 14 1302.2 0 14 24 8 1302.2 0 8 25 4 1302.2 0 4 26 8 1302.2 0 8 27 11 1302.2 0 11 28 18 1302.2 0 18 29 180 1302.2 0 180 30 179 1302.2 0 179 31 23 1302.2 0 23 32 6 1302.2 0 6 33 10 1302.2 0 10 34 11 1302.2 0 11 35 14 1302.2 0 14 36 92 1302.2 0 92 37 19 1302.2 0 19 38 13 1302.2 0 13 39 197 1302.2 0 197 40 14 1302.2 0 14 41 15 1302.2 0 15 42 31 1302.2 0 31 43 57 1302.2 0 57 44 43 1302.2 0 43 45 48 1302.2 0 48 46 11 1302.2 0 11 47 13 1302.2 0 13 48 14 1302.2 0 14 49 26 1302.2 0 26 50 19 1302.2 0 19 51 17 1302.2 0 17 52 14 1302.2 0 14 53 19 1302.2 0 19 54 15 1302.2 0 15 55 25 1302.2 0 25 56 17 1302.2 0 17 57 13 1302.2 0 13 58 8 1302.2 0 8 59 8 1302.2 0 8 60 19 1302.2 0 19 61 11 1302.2 0 11 62 19 1302.2 0 19 63 8 1302.2 0 8 64 15 1302.2 0 15 65 18 1302.2 0 18 66 15 1302.2 0 15 67 19 1302.2 0 19 68 21 1302.2 0 21 69 27 1302.2 0 27 70 381 1302.2 0 381 71 98 1302.2 0 98 72 210 1302.2 0 210 73 38 1302.2 0 38 74 17 1302.2 0 17 75 43 1302.2 0 43 76 34 1302.2 0 34 77 53 1302.2 0 53 78 192 1302.2 0 192 79 819 1302.2 0 819 80 550 1302.2 0 550 81 997 1302.2 0 997 82 2019 1302.2 0 2019 83 2742 1302.2 0 2742 84 274 1302.2 0 274 85 861 1302.2 0 861 86 206 1302.2 0 206 87 72 1302.2 0 72 88 343 1302.2 0 343 89 475 1302.2 0 475 90 31 1302.2 0 31 91 33 1302.2 0 33 92 1533 1302.2 0 1533 93 1689 1302.2 0 1689 94 1209 1302.2 0 1209 95 987 1302.2 0 987 96 540 1302.2 0 540 97 139 1302.2 0 139 98 1834 1302.2 0 1834 99 5368 1302.2 0 5368 100 1134 1302.2 0 1134 101 287 1302.2 0 287 102 989 1302.2 0 989 103 295 1302.2 0 295 104 497 1302.2 0 497 105 1649 1302.2 0 1649 106 388 1302.2 0 388 107 313 1302.2 0 313 108 938 1302.2 0 938 109 2608 1302.2 0 2608 110 449 1302.2 0 449 111 781 1302.2 0 781 112 770 1302.2 0 770 113 541 1302.2 0 541 114 1598 1302.2 0 1598 115 1410 1302.2 0 1410 116 1234 1302.2 0 1234 117 3462 1302.2 0 3462 118 1793 1302.2 0 1793 119 779 1302.2 0 779 120 1322 1302.2 0 1322 121 951 1302.2 0 951 122 966 1302.2 0 966 123 1070 1302.2 0 1070 124 7001 1302.2 0 7001 125 11322 1302.2 0 11322 126 18319 1302.2 0 18319 127 898 1302.2 0 898 128 635 1302.2 0 635 129 1548 1302.2 0 1548 130 1908 1302.2 0 1908 131 1641 1302.2 0 1641 132 793 1302.2 0 793 133 1095 1302.2 0 1095 134 1275 1302.2 0 1275 135 1806 1302.2 0 1806 136 1536 1302.2 0 1536 137 1395 1302.2 0 1395 138 1270 1302.2 0 1270 139 1175 1302.2 0 1175 140 1798 1302.2 0 1798 141 731 1302.2 0 731 142 662 1302.2 0 662 143 1253 1302.2 0 1253 144 2324 1302.2 0 2324 145 1244 1302.2 0 1244 146 816 1302.2 0 816 147 864 1302.2 0 864 148 1022 1302.2 0 1022 149 1879 1302.2 0 1879 150 2985 1302.2 0 2985 151 5451 1302.2 0 5451 152 835 1302.2 0 835 153 1199 1302.2 0 1199 154 677 1302.2 0 677 155 886 1302.2 0 886 156 3336 1302.2 0 3336 157 1637 1302.2 0 1637 158 1523 1302.2 0 1523 159 974 1302.2 0 974 160 1067 1302.2 0 1067 161 1155 1302.2 0 1155 162 1211 1302.2 0 1211 163 1328 1302.2 0 1328 164 2271 1302.2 0 2271 165 1382 1302.2 0 1382 166 1517 1302.2 0 1517 167 1254 1302.2 0 1254 168 1801 1302.2 0 1801 169 2651 1302.2 0 2651 170 771 1302.2 0 771 171 875 1302.2 0 875 172 1362 1302.2 0 1362 173 1650 1302.2 0 1650 174 485 1302.2 0 485 175 608 1302.2 0 608 176 726 1302.2 0 726 177 963 1302.2 0 963 178 1395 1302.2 0 1395 179 1378 1302.2 0 1378 180 1219 1302.2 0 1219 181 2851 1302.2 0 2851 182 1968 1302.2 0 1968 183 2909 1302.2 0 2909 184 1894 1302.2 0 1894 185 1788 1302.2 0 1788 186 1546 1302.2 0 1546 187 786 1302.2 0 786 188 794 1302.2 0 794 189 1259 1302.2 0 1259 190 1821 1302.2 0 1821 191 1488 1302.2 0 1488 192 1229 1302.2 0 1229 193 1500 1302.2 0 1500 194 855 1302.2 0 855 195 866 1302.2 0 866 196 2097 1302.2 0 2097 197 3525 1302.2 0 3525 198 1273 1302.2 0 1273 199 700 1302.2 0 700 200 391 1302.2 0 391 201 941 1302.2 0 941 202 2136 1302.2 0 2136 203 1847 1302.2 0 1847 204 1413 1302.2 0 1413 205 2185 1302.2 0 2185 206 1554 1302.2 0 1554 207 1208 1302.2 0 1208 208 1802 1302.2 0 1802 209 2009 1302.2 0 2009 210 1740 1302.2 0 1740 211 1690 1302.2 0 1690 212 1563 1302.2 0 1563 213 1758 1302.2 0 1758 214 1803 1302.2 0 1803 215 1643 1302.2 0 1643 216 2911 1302.2 0 2911 217 2434 1302.2 0 2434 218 2857 1302.2 0 2857 219 1540 1302.2 0 1540 220 943 1302.2 0 943 221 1374 1302.2 0 1374 222 2653 1302.2 0 2653 223 2580 1302.2 0 2580 224 2491 1302.2 0 2491 225 19314 1302.2 0 19314 226 7185 1302.2 0 7185 227 10468 1302.2 0 10468 228 1729 1302.2 0 1729 229 1505 1302.2 0 1505 230 1541 1302.2 0 1541 231 1125 1302.2 0 1125 232 1096 1302.2 0 1096 233 2229 1302.2 0 2229 234 3529 1302.2 0 3529 235 2111 1302.2 0 2111 236 2790 1302.2 0 2790 237 2528 1302.2 0 2528 238 1619 1302.2 0 1619 239 1103 1302.2 0 1103 240 1043 1302.2 0 1043 241 1169 1302.2 0 1169 242 1476 1302.2 0 1476 243 1486 1302.2 0 1486 244 1339 1302.2 0 1339 245 1843 1302.2 0 1843 246 1749 1302.2 0 1749 247 1250 1302.2 0 1250 248 828 1302.2 0 828 249 629 1302.2 0 629 250 1276 1302.2 0 1276 251 2240 1302.2 0 2240 252 1462 1302.2 0 1462 253 906 1302.2 0 906 254 1331 1302.2 0 1331 255 1696 1302.2 0 1696 256 2152 1302.2 0 2152 257 1294 1302.2 0 1294 258 1006 1302.2 0 1006 259 1109 1302.2 0 1109 260 1874 1302.2 0 1874 261 2179 1302.2 0 2179 262 2480 1302.2 0 2480 263 1561 1302.2 0 1561 264 1504 1302.2 0 1504 265 1569 1302.2 0 1569 266 2370 1302.2 0 2370 267 2004 1302.2 0 2004 268 1202 1302.2 0 1202 269 985 1302.2 0 985 270 2134 1302.2 0 2134 271 2127 1302.2 0 2127 272 2314 1302.2 0 2314 273 2143 1302.2 0 2143 274 1947 1302.2 0 1947 275 1020 1302.2 0 1020 276 1200 1302.2 0 1200 277 1863 1302.2 0 1863 278 1860 1302.2 0 1860 279 1851 1302.2 0 1851 280 1028 1302.2 0 1028 281 1015 1302.2 0 1015 282 1699 1302.2 0 1699 283 1881 1302.2 0 1881 284 1754 1302.2 0 1754 285 1960 1302.2 0 1960 286 1442 1302.2 0 1442 287 1384 1302.2 0 1384 288 1173 1302.2 0 1173 289 1313 1302.2 0 1313 290 1891 1302.2 0 1891 291 2347 1302.2 0 2347 292 2681 1302.2 0 2681 293 1668 1302.2 0 1668 294 1131 1302.2 0 1131 295 1978 1302.2 0 1978 296 3577 1302.2 0 3577 297 2261 1302.2 0 2261 298 2376 1302.2 0 2376 299 3478 1302.2 0 3478 300 2385 1302.2 0 2385 301 1677 1302.2 0 1677 Finished in 8.99 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 206,874 Reads with adapters: 8,956 (4.3%) Reads that were too short: 66 (0.0%) Reads written (passing filters): 8,890 (4.3%) Total basepairs processed: 34,610,404 bp Total written (filtered): 2,415,226 bp (7.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8956 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 0 0 1 19 23 0.0 3 0 22 1 20 1936 0.0 4 1821 113 2 21 6826 0.0 4 6743 79 3 1 22 20 0.0 4 1 8 1 4 6 23 8 0.0 4 1 0 0 3 4 24 4 0.0 4 0 0 1 1 2 25 41 0.0 4 30 9 1 0 1 26 2 0.0 4 0 0 0 0 2 27 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 2 0.0 4 0 0 0 0 2 40 1 0.0 4 0 0 0 0 1 42 2 0.0 4 0 0 0 0 2 45 2 0.0 4 0 0 0 0 2 50 2 0.0 4 0 0 0 0 2 53 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 66 3 0.0 4 0 0 0 0 3 68 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 76 2 0.0 4 0 0 0 0 2 79 6 0.0 4 0 0 0 0 6 82 4 0.0 4 0 0 0 0 4 87 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 0 3 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 7 0.0 4 0 0 0 0 7 103 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 128 4 0.0 4 0 0 0 1 3 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 171 2 0.0 4 0 0 0 0 2 172 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 8,890 Reads with adapters: 271 (3.0%) Reads written (passing filters): 8,890 (100.0%) Total basepairs processed: 2,415,226 bp Total written (filtered): 2,396,813 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 271 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 21.4% G: 73.1% T: 3.0% none/other: 0.4% Overview of removed sequences length count expect max.err error counts 3 3 138.9 0 3 4 1 34.7 0 1 6 1 2.2 0 1 9 26 0.0 0 0 26 10 28 0.0 1 0 28 11 144 0.0 1 0 144 215 26 0.0 1 21 5 216 1 0.0 1 1 219 1 0.0 1 1 242 7 0.0 1 7 243 1 0.0 1 1 249 1 0.0 1 1 256 18 0.0 1 15 3 257 3 0.0 1 3 263 1 0.0 1 1 266 1 0.0 1 1 272 7 0.0 1 7 273 1 0.0 1 1