This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.72 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 278,053 (9.5%) Reads that were too short: 109,915 (3.8%) Reads written (passing filters): 168,138 (5.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 29,102,037 bp (3.3%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 278053 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 129 714.6 0 129 7 4897 714.6 0 4897 8 50 714.6 0 50 9 30 714.6 0 30 10 27 714.6 0 27 11 36 714.6 0 36 12 33 714.6 0 33 13 39 714.6 0 39 14 23 714.6 0 23 15 17 714.6 0 17 16 19 714.6 0 19 17 29 714.6 0 29 18 31 714.6 0 31 19 31 714.6 0 31 20 41 714.6 0 41 21 33 714.6 0 33 22 33 714.6 0 33 23 71 714.6 0 71 24 32 714.6 0 32 25 16 714.6 0 16 26 33 714.6 0 33 27 59 714.6 0 59 28 57 714.6 0 57 29 198 714.6 0 198 30 207 714.6 0 207 31 58 714.6 0 58 32 37 714.6 0 37 33 23 714.6 0 23 34 46 714.6 0 46 35 29 714.6 0 29 36 50 714.6 0 50 37 47 714.6 0 47 38 32 714.6 0 32 39 60 714.6 0 60 40 30 714.6 0 30 41 22 714.6 0 22 42 33 714.6 0 33 43 39 714.6 0 39 44 41 714.6 0 41 45 39 714.6 0 39 46 40 714.6 0 40 47 39 714.6 0 39 48 36 714.6 0 36 49 34 714.6 0 34 50 27 714.6 0 27 51 22 714.6 0 22 52 24 714.6 0 24 53 29 714.6 0 29 54 52 714.6 0 52 55 32 714.6 0 32 56 35 714.6 0 35 57 217 714.6 0 217 58 80 714.6 0 80 59 44 714.6 0 44 60 44 714.6 0 44 61 61 714.6 0 61 62 33 714.6 0 33 63 41 714.6 0 41 64 40 714.6 0 40 65 36 714.6 0 36 66 49 714.6 0 49 67 45 714.6 0 45 68 50 714.6 0 50 69 66 714.6 0 66 70 662 714.6 0 662 71 1602 714.6 0 1602 72 220 714.6 0 220 73 132 714.6 0 132 74 194 714.6 0 194 75 200 714.6 0 200 76 763 714.6 0 763 77 455 714.6 0 455 78 870 714.6 0 870 79 1181 714.6 0 1181 80 855 714.6 0 855 81 2561 714.6 0 2561 82 1970 714.6 0 1970 83 1741 714.6 0 1741 84 770 714.6 0 770 85 1034 714.6 0 1034 86 641 714.6 0 641 87 571 714.6 0 571 88 220 714.6 0 220 89 472 714.6 0 472 90 616 714.6 0 616 91 689 714.6 0 689 92 869 714.6 0 869 93 886 714.6 0 886 94 1156 714.6 0 1156 95 824 714.6 0 824 96 512 714.6 0 512 97 661 714.6 0 661 98 1254 714.6 0 1254 99 1730 714.6 0 1730 100 837 714.6 0 837 101 596 714.6 0 596 102 677 714.6 0 677 103 428 714.6 0 428 104 255 714.6 0 255 105 181 714.6 0 181 106 330 714.6 0 330 107 1351 714.6 0 1351 108 887 714.6 0 887 109 708 714.6 0 708 110 867 714.6 0 867 111 1222 714.6 0 1222 112 925 714.6 0 925 113 1017 714.6 0 1017 114 1413 714.6 0 1413 115 1638 714.6 0 1638 116 1380 714.6 0 1380 117 1312 714.6 0 1312 118 1021 714.6 0 1021 119 969 714.6 0 969 120 1000 714.6 0 1000 121 885 714.6 0 885 122 819 714.6 0 819 123 1158 714.6 0 1158 124 9391 714.6 0 9391 125 13780 714.6 0 13780 126 18113 714.6 0 18113 127 998 714.6 0 998 128 796 714.6 0 796 129 1546 714.6 0 1546 130 1288 714.6 0 1288 131 1011 714.6 0 1011 132 709 714.6 0 709 133 878 714.6 0 878 134 804 714.6 0 804 135 944 714.6 0 944 136 965 714.6 0 965 137 735 714.6 0 735 138 720 714.6 0 720 139 925 714.6 0 925 140 967 714.6 0 967 141 812 714.6 0 812 142 769 714.6 0 769 143 666 714.6 0 666 144 803 714.6 0 803 145 736 714.6 0 736 146 776 714.6 0 776 147 788 714.6 0 788 148 882 714.6 0 882 149 2136 714.6 0 2136 150 3692 714.6 0 3692 151 3941 714.6 0 3941 152 858 714.6 0 858 153 710 714.6 0 710 154 813 714.6 0 813 155 777 714.6 0 777 156 1008 714.6 0 1008 157 889 714.6 0 889 158 774 714.6 0 774 159 663 714.6 0 663 160 659 714.6 0 659 161 801 714.6 0 801 162 838 714.6 0 838 163 783 714.6 0 783 164 979 714.6 0 979 165 895 714.6 0 895 166 871 714.6 0 871 167 662 714.6 0 662 168 707 714.6 0 707 169 1125 714.6 0 1125 170 765 714.6 0 765 171 606 714.6 0 606 172 530 714.6 0 530 173 621 714.6 0 621 174 572 714.6 0 572 175 612 714.6 0 612 176 715 714.6 0 715 177 844 714.6 0 844 178 910 714.6 0 910 179 773 714.6 0 773 180 713 714.6 0 713 181 1484 714.6 0 1484 182 1312 714.6 0 1312 183 999 714.6 0 999 184 830 714.6 0 830 185 987 714.6 0 987 186 917 714.6 0 917 187 689 714.6 0 689 188 790 714.6 0 790 189 842 714.6 0 842 190 960 714.6 0 960 191 783 714.6 0 783 192 856 714.6 0 856 193 937 714.6 0 937 194 531 714.6 0 531 195 572 714.6 0 572 196 1238 714.6 0 1238 197 1463 714.6 0 1463 198 950 714.6 0 950 199 535 714.6 0 535 200 431 714.6 0 431 201 868 714.6 0 868 202 1250 714.6 0 1250 203 968 714.6 0 968 204 947 714.6 0 947 205 1158 714.6 0 1158 206 1023 714.6 0 1023 207 963 714.6 0 963 208 1048 714.6 0 1048 209 1013 714.6 0 1013 210 1142 714.6 0 1142 211 974 714.6 0 974 212 949 714.6 0 949 213 857 714.6 0 857 214 999 714.6 0 999 215 917 714.6 0 917 216 987 714.6 0 987 217 920 714.6 0 920 218 1068 714.6 0 1068 219 792 714.6 0 792 220 662 714.6 0 662 221 838 714.6 0 838 222 1214 714.6 0 1214 223 1468 714.6 0 1468 224 1825 714.6 0 1825 225 5483 714.6 0 5483 226 9531 714.6 0 9531 227 2642 714.6 0 2642 228 902 714.6 0 902 229 547 714.6 0 547 230 745 714.6 0 745 231 904 714.6 0 904 232 873 714.6 0 873 233 1006 714.6 0 1006 234 1563 714.6 0 1563 235 1559 714.6 0 1559 236 898 714.6 0 898 237 994 714.6 0 994 238 1227 714.6 0 1227 239 879 714.6 0 879 240 688 714.6 0 688 241 1057 714.6 0 1057 242 1004 714.6 0 1004 243 891 714.6 0 891 244 907 714.6 0 907 245 1109 714.6 0 1109 246 1030 714.6 0 1030 247 771 714.6 0 771 248 572 714.6 0 572 249 473 714.6 0 473 250 731 714.6 0 731 251 1117 714.6 0 1117 252 932 714.6 0 932 253 540 714.6 0 540 254 807 714.6 0 807 255 814 714.6 0 814 256 935 714.6 0 935 257 877 714.6 0 877 258 739 714.6 0 739 259 592 714.6 0 592 260 982 714.6 0 982 261 1238 714.6 0 1238 262 1110 714.6 0 1110 263 922 714.6 0 922 264 795 714.6 0 795 265 683 714.6 0 683 266 968 714.6 0 968 267 922 714.6 0 922 268 621 714.6 0 621 269 592 714.6 0 592 270 1238 714.6 0 1238 271 1245 714.6 0 1245 272 965 714.6 0 965 273 1091 714.6 0 1091 274 918 714.6 0 918 275 522 714.6 0 522 276 797 714.6 0 797 277 1247 714.6 0 1247 278 1031 714.6 0 1031 279 748 714.6 0 748 280 599 714.6 0 599 281 714 714.6 0 714 282 926 714.6 0 926 283 928 714.6 0 928 284 899 714.6 0 899 285 1163 714.6 0 1163 286 722 714.6 0 722 287 503 714.6 0 503 288 883 714.6 0 883 289 938 714.6 0 938 290 1020 714.6 0 1020 291 1145 714.6 0 1145 292 841 714.6 0 841 293 816 714.6 0 816 294 1013 714.6 0 1013 295 1059 714.6 0 1059 296 1290 714.6 0 1290 297 1514 714.6 0 1514 298 1327 714.6 0 1327 299 1221 714.6 0 1221 300 690 714.6 0 690 301 748 714.6 0 748 Finished in 5.54 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 168,138 Reads with adapters: 5,621 (3.3%) Reads that were too short: 107 (0.1%) Reads written (passing filters): 5,514 (3.3%) Total basepairs processed: 29,102,037 bp Total written (filtered): 1,434,588 bp (4.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5621 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 80 0.0 3 0 78 1 1 17 4841 0.0 3 4707 114 11 9 18 9 0.0 3 0 6 2 1 19 8 0.0 3 1 0 4 3 20 5 0.0 3 1 0 0 4 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 29 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 42 156 0.0 3 0 0 0 156 44 1 0.0 3 0 0 0 1 45 20 0.0 3 0 0 0 20 46 2 0.0 3 0 0 1 1 52 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 66 6 0.0 3 0 0 0 6 68 337 0.0 3 0 0 1 336 69 4 0.0 3 0 0 2 2 76 3 0.0 3 0 0 0 3 80 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 1 88 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 93 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 102 6 0.0 3 0 0 0 6 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 5 0.0 3 0 0 0 5 115 2 0.0 3 0 0 1 1 116 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 4 0.0 3 0 0 0 4 143 7 0.0 3 0 0 0 7 144 38 0.0 3 0 0 1 37 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 5,514 Reads with adapters: 428 (7.8%) Reads written (passing filters): 5,514 (100.0%) Total basepairs processed: 1,434,588 bp Total written (filtered): 1,433,303 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 428 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.7% C: 43.5% G: 22.2% T: 5.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 427 86.2 0 427 4 1 21.5 0 1