This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.31 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 94,809 (3.2%) Reads that were too short: 30,755 (1.1%) Reads written (passing filters): 64,054 (2.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 14,382,774 bp (1.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 94809 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 840 714.6 0 840 7 13987 714.6 0 13987 8 13935 714.6 0 13935 9 76 714.6 0 76 10 68 714.6 0 68 11 63 714.6 0 63 12 52 714.6 0 52 13 66 714.6 0 66 14 59 714.6 0 59 15 63 714.6 0 63 16 58 714.6 0 58 17 60 714.6 0 60 18 53 714.6 0 53 19 56 714.6 0 56 20 56 714.6 0 56 21 55 714.6 0 55 22 57 714.6 0 57 23 55 714.6 0 55 24 61 714.6 0 61 25 77 714.6 0 77 26 65 714.6 0 65 27 54 714.6 0 54 28 73 714.6 0 73 29 54 714.6 0 54 30 70 714.6 0 70 31 79 714.6 0 79 32 62 714.6 0 62 33 64 714.6 0 64 34 59 714.6 0 59 35 72 714.6 0 72 36 52 714.6 0 52 37 47 714.6 0 47 38 51 714.6 0 51 39 51 714.6 0 51 40 71 714.6 0 71 41 70 714.6 0 70 42 61 714.6 0 61 43 48 714.6 0 48 44 61 714.6 0 61 45 56 714.6 0 56 46 45 714.6 0 45 47 61 714.6 0 61 48 47 714.6 0 47 49 63 714.6 0 63 50 48 714.6 0 48 51 49 714.6 0 49 52 72 714.6 0 72 53 1030 714.6 0 1030 54 83 714.6 0 83 55 116 714.6 0 116 56 34 714.6 0 34 57 45 714.6 0 45 58 49 714.6 0 49 59 64 714.6 0 64 60 43 714.6 0 43 61 42 714.6 0 42 62 72 714.6 0 72 63 89 714.6 0 89 64 828 714.6 0 828 65 196 714.6 0 196 66 208 714.6 0 208 67 43 714.6 0 43 68 70 714.6 0 70 69 78 714.6 0 78 70 40 714.6 0 40 71 93 714.6 0 93 72 45 714.6 0 45 73 44 714.6 0 44 74 50 714.6 0 50 75 99 714.6 0 99 76 48 714.6 0 48 77 51 714.6 0 51 78 47 714.6 0 47 79 69 714.6 0 69 80 89 714.6 0 89 81 92 714.6 0 92 82 58 714.6 0 58 83 181 714.6 0 181 84 202 714.6 0 202 85 104 714.6 0 104 86 106 714.6 0 106 87 171 714.6 0 171 88 122 714.6 0 122 89 327 714.6 0 327 90 374 714.6 0 374 91 134 714.6 0 134 92 337 714.6 0 337 93 353 714.6 0 353 94 121 714.6 0 121 95 174 714.6 0 174 96 137 714.6 0 137 97 60 714.6 0 60 98 62 714.6 0 62 99 116 714.6 0 116 100 160 714.6 0 160 101 172 714.6 0 172 102 84 714.6 0 84 103 88 714.6 0 88 104 90 714.6 0 90 105 73 714.6 0 73 106 49 714.6 0 49 107 35 714.6 0 35 108 52 714.6 0 52 109 57 714.6 0 57 110 65 714.6 0 65 111 109 714.6 0 109 112 90 714.6 0 90 113 119 714.6 0 119 114 126 714.6 0 126 115 119 714.6 0 119 116 179 714.6 0 179 117 255 714.6 0 255 118 150 714.6 0 150 119 103 714.6 0 103 120 102 714.6 0 102 121 162 714.6 0 162 122 186 714.6 0 186 123 178 714.6 0 178 124 144 714.6 0 144 125 127 714.6 0 127 126 143 714.6 0 143 127 126 714.6 0 126 128 161 714.6 0 161 129 210 714.6 0 210 130 274 714.6 0 274 131 313 714.6 0 313 132 297 714.6 0 297 133 169 714.6 0 169 134 159 714.6 0 159 135 273 714.6 0 273 136 317 714.6 0 317 137 415 714.6 0 415 138 179 714.6 0 179 139 183 714.6 0 183 140 137 714.6 0 137 141 159 714.6 0 159 142 231 714.6 0 231 143 248 714.6 0 248 144 202 714.6 0 202 145 160 714.6 0 160 146 198 714.6 0 198 147 443 714.6 0 443 148 374 714.6 0 374 149 317 714.6 0 317 150 242 714.6 0 242 151 231 714.6 0 231 152 229 714.6 0 229 153 269 714.6 0 269 154 241 714.6 0 241 155 350 714.6 0 350 156 408 714.6 0 408 157 473 714.6 0 473 158 559 714.6 0 559 159 387 714.6 0 387 160 337 714.6 0 337 161 339 714.6 0 339 162 332 714.6 0 332 163 346 714.6 0 346 164 212 714.6 0 212 165 157 714.6 0 157 166 302 714.6 0 302 167 476 714.6 0 476 168 442 714.6 0 442 169 308 714.6 0 308 170 346 714.6 0 346 171 339 714.6 0 339 172 306 714.6 0 306 173 244 714.6 0 244 174 230 714.6 0 230 175 623 714.6 0 623 176 1101 714.6 0 1101 177 625 714.6 0 625 178 323 714.6 0 323 179 233 714.6 0 233 180 213 714.6 0 213 181 248 714.6 0 248 182 296 714.6 0 296 183 385 714.6 0 385 184 357 714.6 0 357 185 252 714.6 0 252 186 419 714.6 0 419 187 430 714.6 0 430 188 531 714.6 0 531 189 333 714.6 0 333 190 178 714.6 0 178 191 175 714.6 0 175 192 202 714.6 0 202 193 193 714.6 0 193 194 247 714.6 0 247 195 242 714.6 0 242 196 184 714.6 0 184 197 157 714.6 0 157 198 124 714.6 0 124 199 263 714.6 0 263 200 287 714.6 0 287 201 168 714.6 0 168 202 152 714.6 0 152 203 152 714.6 0 152 204 241 714.6 0 241 205 252 714.6 0 252 206 236 714.6 0 236 207 192 714.6 0 192 208 235 714.6 0 235 209 249 714.6 0 249 210 273 714.6 0 273 211 266 714.6 0 266 212 247 714.6 0 247 213 285 714.6 0 285 214 344 714.6 0 344 215 387 714.6 0 387 216 469 714.6 0 469 217 389 714.6 0 389 218 349 714.6 0 349 219 347 714.6 0 347 220 383 714.6 0 383 221 396 714.6 0 396 222 570 714.6 0 570 223 721 714.6 0 721 224 466 714.6 0 466 225 456 714.6 0 456 226 458 714.6 0 458 227 394 714.6 0 394 228 366 714.6 0 366 229 389 714.6 0 389 230 275 714.6 0 275 231 188 714.6 0 188 232 258 714.6 0 258 233 291 714.6 0 291 234 266 714.6 0 266 235 265 714.6 0 265 236 358 714.6 0 358 237 278 714.6 0 278 238 245 714.6 0 245 239 257 714.6 0 257 240 219 714.6 0 219 241 321 714.6 0 321 242 325 714.6 0 325 243 562 714.6 0 562 244 457 714.6 0 457 245 461 714.6 0 461 246 339 714.6 0 339 247 590 714.6 0 590 248 404 714.6 0 404 249 339 714.6 0 339 250 174 714.6 0 174 251 101 714.6 0 101 252 467 714.6 0 467 253 661 714.6 0 661 254 604 714.6 0 604 255 612 714.6 0 612 256 433 714.6 0 433 257 379 714.6 0 379 258 284 714.6 0 284 259 195 714.6 0 195 260 111 714.6 0 111 261 83 714.6 0 83 262 150 714.6 0 150 263 227 714.6 0 227 264 316 714.6 0 316 265 315 714.6 0 315 266 306 714.6 0 306 267 345 714.6 0 345 268 631 714.6 0 631 269 474 714.6 0 474 270 351 714.6 0 351 271 224 714.6 0 224 272 249 714.6 0 249 273 340 714.6 0 340 274 403 714.6 0 403 275 311 714.6 0 311 276 150 714.6 0 150 277 136 714.6 0 136 278 159 714.6 0 159 279 237 714.6 0 237 280 375 714.6 0 375 281 303 714.6 0 303 282 227 714.6 0 227 283 259 714.6 0 259 284 189 714.6 0 189 285 298 714.6 0 298 286 298 714.6 0 298 287 419 714.6 0 419 288 237 714.6 0 237 289 151 714.6 0 151 290 138 714.6 0 138 291 145 714.6 0 145 292 270 714.6 0 270 293 412 714.6 0 412 294 289 714.6 0 289 295 151 714.6 0 151 296 123 714.6 0 123 297 108 714.6 0 108 298 129 714.6 0 129 299 129 714.6 0 129 300 201 714.6 0 201 301 114 714.6 0 114 Finished in 2.60 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 64,054 Reads with adapters: 14,296 (22.3%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 14,273 (22.3%) Total basepairs processed: 14,382,774 bp Total written (filtered): 3,950,321 bp (27.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14296 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 5 0.0 3 0 0 5 16 103 0.0 3 0 93 6 4 17 13830 0.0 3 13395 377 46 12 18 31 0.0 3 5 18 6 2 19 26 0.0 3 0 3 15 8 20 254 0.0 3 242 4 2 6 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 3 0.0 3 0 0 0 3 44 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 1 1 77 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 1 97 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 4 0.0 3 0 0 0 4 233 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.51 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 14,273 Reads with adapters: 1,119 (7.8%) Reads written (passing filters): 14,273 (100.0%) Total basepairs processed: 3,950,321 bp Total written (filtered): 3,946,963 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1119 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.0% C: 59.0% G: 17.5% T: 0.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1118 223.0 0 1118 4 1 55.8 0 1