This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.96 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,708,680 Reads with adapters: 281,195 (10.4%) Reads that were too short: 10,808 (0.4%) Reads written (passing filters): 270,387 (10.0%) Total basepairs processed: 815,312,680 bp Total written (filtered): 65,796,086 bp (8.1%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 281195 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 159 661.3 0 159 7 1142 661.3 0 1142 8 181 661.3 0 181 9 249 661.3 0 249 10 323 661.3 0 323 11 194 661.3 0 194 12 181 661.3 0 181 13 385 661.3 0 385 14 552 661.3 0 552 15 532 661.3 0 532 16 346 661.3 0 346 17 471 661.3 0 471 18 518 661.3 0 518 19 668 661.3 0 668 20 9355 661.3 0 9355 21 21018 661.3 0 21018 22 34556 661.3 0 34556 23 246 661.3 0 246 24 138 661.3 0 138 25 129 661.3 0 129 26 75 661.3 0 75 27 85 661.3 0 85 28 71 661.3 0 71 29 110 661.3 0 110 30 85 661.3 0 85 31 161 661.3 0 161 32 274 661.3 0 274 33 348 661.3 0 348 34 331 661.3 0 331 35 722 661.3 0 722 36 1658 661.3 0 1658 37 406 661.3 0 406 38 540 661.3 0 540 39 1083 661.3 0 1083 40 499 661.3 0 499 41 489 661.3 0 489 42 706 661.3 0 706 43 775 661.3 0 775 44 1214 661.3 0 1214 45 870 661.3 0 870 46 1151 661.3 0 1151 47 1315 661.3 0 1315 48 1957 661.3 0 1957 49 30476 661.3 0 30476 50 47721 661.3 0 47721 51 71526 661.3 0 71526 52 462 661.3 0 462 53 49 661.3 0 49 54 48 661.3 0 48 55 36 661.3 0 36 56 80 661.3 0 80 57 45 661.3 0 45 58 62 661.3 0 62 59 36 661.3 0 36 60 47 661.3 0 47 61 31 661.3 0 31 62 58 661.3 0 58 63 175 661.3 0 175 64 260 661.3 0 260 65 602 661.3 0 602 66 29 661.3 0 29 67 48 661.3 0 48 68 42 661.3 0 42 69 31 661.3 0 31 70 25 661.3 0 25 71 27 661.3 0 27 72 34 661.3 0 34 73 33 661.3 0 33 74 26 661.3 0 26 75 26 661.3 0 26 76 32 661.3 0 32 77 30 661.3 0 30 78 37 661.3 0 37 79 28 661.3 0 28 80 101 661.3 0 101 81 26 661.3 0 26 82 32 661.3 0 32 83 35 661.3 0 35 84 64 661.3 0 64 85 50 661.3 0 50 86 55 661.3 0 55 87 45 661.3 0 45 88 50 661.3 0 50 89 27 661.3 0 27 90 47 661.3 0 47 91 32 661.3 0 32 92 48 661.3 0 48 93 41 661.3 0 41 94 39 661.3 0 39 95 32 661.3 0 32 96 34 661.3 0 34 97 32 661.3 0 32 98 39 661.3 0 39 99 22 661.3 0 22 100 13 661.3 0 13 101 29 661.3 0 29 102 37 661.3 0 37 103 20 661.3 0 20 104 32 661.3 0 32 105 22 661.3 0 22 106 37 661.3 0 37 107 34 661.3 0 34 108 27 661.3 0 27 109 26 661.3 0 26 110 34 661.3 0 34 111 29 661.3 0 29 112 39 661.3 0 39 113 29 661.3 0 29 114 26 661.3 0 26 115 29 661.3 0 29 116 36 661.3 0 36 117 115 661.3 0 115 118 89 661.3 0 89 119 117 661.3 0 117 120 100 661.3 0 100 121 34 661.3 0 34 122 26 661.3 0 26 123 39 661.3 0 39 124 73 661.3 0 73 125 47 661.3 0 47 126 36 661.3 0 36 127 32 661.3 0 32 128 25 661.3 0 25 129 32 661.3 0 32 130 35 661.3 0 35 131 78 661.3 0 78 132 32 661.3 0 32 133 29 661.3 0 29 134 34 661.3 0 34 135 33 661.3 0 33 136 30 661.3 0 30 137 32 661.3 0 32 138 26 661.3 0 26 139 34 661.3 0 34 140 42 661.3 0 42 141 46 661.3 0 46 142 34 661.3 0 34 143 23 661.3 0 23 144 53 661.3 0 53 145 42 661.3 0 42 146 58 661.3 0 58 147 34 661.3 0 34 148 40 661.3 0 40 149 30 661.3 0 30 150 32 661.3 0 32 151 21 661.3 0 21 152 37 661.3 0 37 153 51 661.3 0 51 154 27 661.3 0 27 155 34 661.3 0 34 156 37 661.3 0 37 157 73 661.3 0 73 158 44 661.3 0 44 159 64 661.3 0 64 160 42 661.3 0 42 161 45 661.3 0 45 162 36 661.3 0 36 163 29 661.3 0 29 164 41 661.3 0 41 165 45 661.3 0 45 166 39 661.3 0 39 167 51 661.3 0 51 168 48 661.3 0 48 169 45 661.3 0 45 170 93 661.3 0 93 171 47 661.3 0 47 172 33 661.3 0 33 173 64 661.3 0 64 174 49 661.3 0 49 175 41 661.3 0 41 176 70 661.3 0 70 177 91 661.3 0 91 178 95 661.3 0 95 179 173 661.3 0 173 180 262 661.3 0 262 181 592 661.3 0 592 182 8568 661.3 0 8568 183 644 661.3 0 644 184 1496 661.3 0 1496 185 130 661.3 0 130 186 74 661.3 0 74 187 73 661.3 0 73 188 130 661.3 0 130 189 120 661.3 0 120 190 130 661.3 0 130 191 129 661.3 0 129 192 151 661.3 0 151 193 509 661.3 0 509 194 882 661.3 0 882 195 2627 661.3 0 2627 196 2545 661.3 0 2545 197 8147 661.3 0 8147 198 134 661.3 0 134 199 57 661.3 0 57 200 43 661.3 0 43 201 37 661.3 0 37 202 27 661.3 0 27 203 31 661.3 0 31 204 37 661.3 0 37 205 30 661.3 0 30 206 43 661.3 0 43 207 33 661.3 0 33 208 37 661.3 0 37 209 56 661.3 0 56 210 27 661.3 0 27 211 42 661.3 0 42 212 28 661.3 0 28 213 80 661.3 0 80 214 101 661.3 0 101 215 220 661.3 0 220 216 144 661.3 0 144 217 71 661.3 0 71 218 94 661.3 0 94 219 98 661.3 0 98 220 144 661.3 0 144 221 161 661.3 0 161 222 194 661.3 0 194 223 61 661.3 0 61 224 36 661.3 0 36 225 44 661.3 0 44 226 59 661.3 0 59 227 49 661.3 0 49 228 48 661.3 0 48 229 44 661.3 0 44 230 41 661.3 0 41 231 54 661.3 0 54 232 46 661.3 0 46 233 48 661.3 0 48 234 74 661.3 0 74 235 63 661.3 0 63 236 74 661.3 0 74 237 60 661.3 0 60 238 66 661.3 0 66 239 95 661.3 0 95 240 251 661.3 0 251 241 356 661.3 0 356 242 109 661.3 0 109 243 19 661.3 0 19 244 34 661.3 0 34 245 51 661.3 0 51 246 100 661.3 0 100 247 79 661.3 0 79 248 69 661.3 0 69 249 52 661.3 0 52 250 123 661.3 0 123 251 133 661.3 0 133 252 229 661.3 0 229 253 114 661.3 0 114 254 58 661.3 0 58 255 59 661.3 0 59 256 84 661.3 0 84 257 99 661.3 0 99 258 96 661.3 0 96 259 77 661.3 0 77 260 88 661.3 0 88 261 98 661.3 0 98 262 84 661.3 0 84 263 159 661.3 0 159 264 370 661.3 0 370 265 401 661.3 0 401 266 211 661.3 0 211 267 203 661.3 0 203 268 89 661.3 0 89 269 57 661.3 0 57 270 81 661.3 0 81 271 28 661.3 0 28 272 63 661.3 0 63 273 44 661.3 0 44 274 48 661.3 0 48 275 57 661.3 0 57 276 38 661.3 0 38 277 36 661.3 0 36 278 56 661.3 0 56 279 55 661.3 0 55 280 50 661.3 0 50 281 43 661.3 0 43 282 56 661.3 0 56 283 73 661.3 0 73 284 92 661.3 0 92 285 184 661.3 0 184 286 95 661.3 0 95 287 98 661.3 0 98 288 306 661.3 0 306 289 790 661.3 0 790 290 1081 661.3 0 1081 291 131 661.3 0 131 292 72 661.3 0 72 293 189 661.3 0 189 294 99 661.3 0 99 295 89 661.3 0 89 296 36 661.3 0 36 297 23 661.3 0 23 298 31 661.3 0 31 299 45 661.3 0 45 300 66 661.3 0 66 301 41 661.3 0 41 Finished in 10.64 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 270,387 Reads with adapters: 3,864 (1.4%) Reads that were too short: 1,389 (0.5%) Reads written (passing filters): 2,475 (0.9%) Total basepairs processed: 65,796,086 bp Total written (filtered): 431,814 bp (0.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3864 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 4 0.0 3 0 4 17 779 0.0 3 744 31 2 2 18 4 0.0 3 0 3 1 19 8 0.0 3 4 1 3 20 2 0.0 3 0 0 0 2 22 1 0.0 3 0 0 1 23 1 0.0 3 1 24 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 5 0.0 3 3 0 0 2 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 46 20 0.0 3 0 0 0 20 47 3 0.0 3 0 0 0 3 49 2 0.0 3 0 0 0 2 51 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 75 0.0 3 0 0 1 74 74 7 0.0 3 1 0 0 6 76 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 4 0.0 3 0 0 0 4 89 4 0.0 3 0 0 1 3 90 13 0.0 3 0 0 0 13 91 3 0.0 3 0 0 1 2 92 30 0.0 3 0 0 1 29 93 20 0.0 3 0 0 4 16 94 2 0.0 3 0 0 0 2 97 9 0.0 3 0 0 0 9 98 4 0.0 3 0 0 0 4 99 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 102 7 0.0 3 0 0 0 7 103 2 0.0 3 0 0 1 1 104 4 0.0 3 0 0 0 4 107 5 0.0 3 0 0 1 4 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 117 8 0.0 3 0 0 0 8 118 4 0.0 3 0 0 0 4 121 1 0.0 3 0 0 0 1 122 270 0.0 3 0 0 1 269 123 186 0.0 3 0 0 1 185 124 155 0.0 3 0 0 0 155 125 48 0.0 3 0 0 1 47 126 21 0.0 3 0 0 0 21 127 18 0.0 3 0 0 0 18 128 72 0.0 3 0 0 0 72 129 4 0.0 3 0 0 0 4 130 3 0.0 3 0 0 0 3 131 10 0.0 3 0 0 0 10 132 4 0.0 3 0 0 0 4 133 2 0.0 3 0 0 0 2 134 11 0.0 3 0 0 0 11 135 7 0.0 3 0 0 0 7 137 41 0.0 3 0 0 0 41 138 12 0.0 3 0 0 0 12 139 36 0.0 3 0 0 0 36 140 15 0.0 3 0 0 0 15 141 4 0.0 3 0 0 0 4 142 124 0.0 3 0 0 0 124 143 13 0.0 3 0 0 0 13 144 1 0.0 3 0 0 0 1 145 26 0.0 3 0 0 2 24 146 8 0.0 3 0 0 0 8 147 2 0.0 3 0 0 0 2 148 5 0.0 3 0 0 0 5 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 66 0.0 3 0 0 0 66 152 17 0.0 3 0 0 0 17 153 26 0.0 3 0 0 0 26 154 6 0.0 3 0 0 0 6 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 157 9 0.0 3 0 0 0 9 161 5 0.0 3 0 0 0 5 163 4 0.0 3 0 0 0 4 164 3 0.0 3 0 0 0 3 165 2 0.0 3 0 0 0 2 166 4 0.0 3 0 0 0 4 167 3 0.0 3 1 0 0 2 168 7 0.0 3 0 0 0 7 169 4 0.0 3 0 0 0 4 170 4 0.0 3 0 0 0 4 171 162 0.0 3 0 0 0 162 172 61 0.0 3 0 0 1 60 173 4 0.0 3 0 0 0 4 174 32 0.0 3 0 0 0 32 175 5 0.0 3 0 0 0 5 176 1 0.0 3 0 0 0 1 177 3 0.0 3 0 0 0 3 178 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 196 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 1 1 222 1 0.0 3 0 0 0 1 223 3 0.0 3 0 0 0 3 225 3 0.0 3 0 0 0 3 229 1 0.0 3 0 0 1 231 2 0.0 3 0 0 0 2 232 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 234 389 0.0 3 0 0 8 381 235 91 0.0 3 0 0 4 87 236 56 0.0 3 0 0 0 56 237 146 0.0 3 0 0 5 141 238 205 0.0 3 0 0 4 201 239 50 0.0 3 0 0 1 49 240 54 0.0 3 0 0 1 53 241 5 0.0 3 0 0 0 5 242 1 0.0 3 0 0 0 1 243 21 0.0 3 0 0 1 20 244 68 0.0 3 0 0 1 67 245 2 0.0 3 0 0 0 2 246 2 0.0 3 0 0 0 2 247 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 250 6 0.0 3 0 0 0 6 251 3 0.0 3 0 0 0 3 253 1 0.0 3 0 0 0 1 254 4 0.0 3 0 0 0 4 256 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 263 35 0.0 3 0 0 0 35 264 10 0.0 3 0 0 0 10 265 8 0.0 3 0 0 0 8 266 11 0.0 3 0 0 0 11 267 13 0.0 3 0 0 1 12 268 4 0.0 3 0 0 0 4 269 3 0.0 3 0 0 0 3 270 2 0.0 3 0 0 0 2 272 9 0.0 3 0 0 0 9 273 19 0.0 3 0 0 1 18 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (28 us/read; 2.12 M reads/minute). === Summary === Total reads processed: 2,475 Reads with adapters: 118 (4.8%) Reads written (passing filters): 2,475 (100.0%) Total basepairs processed: 431,814 bp Total written (filtered): 431,458 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 118 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.9% C: 22.0% G: 6.8% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 116 38.7 0 116 4 2 9.7 0 2