This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.82 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 49,669 (1.7%) Reads that were too short: 17,800 (0.6%) Reads written (passing filters): 31,869 (1.1%) Total basepairs processed: 905,907,359 bp Total written (filtered): 7,006,299 bp (0.8%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 49669 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 389 734.8 0 389 7 1967 734.8 0 1967 8 9493 734.8 0 9493 9 71 734.8 0 71 10 58 734.8 0 58 11 52 734.8 0 52 12 55 734.8 0 55 13 34 734.8 0 34 14 45 734.8 0 45 15 44 734.8 0 44 16 46 734.8 0 46 17 35 734.8 0 35 18 32 734.8 0 32 19 40 734.8 0 40 20 42 734.8 0 42 21 45 734.8 0 45 22 53 734.8 0 53 23 52 734.8 0 52 24 52 734.8 0 52 25 49 734.8 0 49 26 40 734.8 0 40 27 40 734.8 0 40 28 38 734.8 0 38 29 54 734.8 0 54 30 50 734.8 0 50 31 54 734.8 0 54 32 42 734.8 0 42 33 44 734.8 0 44 34 37 734.8 0 37 35 55 734.8 0 55 36 60 734.8 0 60 37 55 734.8 0 55 38 41 734.8 0 41 39 52 734.8 0 52 40 59 734.8 0 59 41 38 734.8 0 38 42 46 734.8 0 46 43 57 734.8 0 57 44 67 734.8 0 67 45 52 734.8 0 52 46 52 734.8 0 52 47 54 734.8 0 54 48 38 734.8 0 38 49 51 734.8 0 51 50 53 734.8 0 53 51 49 734.8 0 49 52 59 734.8 0 59 53 230 734.8 0 230 54 50 734.8 0 50 55 71 734.8 0 71 56 34 734.8 0 34 57 38 734.8 0 38 58 44 734.8 0 44 59 48 734.8 0 48 60 31 734.8 0 31 61 34 734.8 0 34 62 59 734.8 0 59 63 65 734.8 0 65 64 783 734.8 0 783 65 415 734.8 0 415 66 98 734.8 0 98 67 43 734.8 0 43 68 67 734.8 0 67 69 122 734.8 0 122 70 85 734.8 0 85 71 70 734.8 0 70 72 41 734.8 0 41 73 33 734.8 0 33 74 55 734.8 0 55 75 41 734.8 0 41 76 38 734.8 0 38 77 32 734.8 0 32 78 29 734.8 0 29 79 39 734.8 0 39 80 48 734.8 0 48 81 65 734.8 0 65 82 40 734.8 0 40 83 94 734.8 0 94 84 93 734.8 0 93 85 58 734.8 0 58 86 79 734.8 0 79 87 115 734.8 0 115 88 61 734.8 0 61 89 127 734.8 0 127 90 203 734.8 0 203 91 76 734.8 0 76 92 293 734.8 0 293 93 271 734.8 0 271 94 80 734.8 0 80 95 167 734.8 0 167 96 65 734.8 0 65 97 37 734.8 0 37 98 41 734.8 0 41 99 71 734.8 0 71 100 90 734.8 0 90 101 73 734.8 0 73 102 48 734.8 0 48 103 54 734.8 0 54 104 56 734.8 0 56 105 54 734.8 0 54 106 40 734.8 0 40 107 33 734.8 0 33 108 39 734.8 0 39 109 52 734.8 0 52 110 58 734.8 0 58 111 74 734.8 0 74 112 48 734.8 0 48 113 53 734.8 0 53 114 71 734.8 0 71 115 64 734.8 0 64 116 94 734.8 0 94 117 113 734.8 0 113 118 82 734.8 0 82 119 61 734.8 0 61 120 63 734.8 0 63 121 90 734.8 0 90 122 95 734.8 0 95 123 92 734.8 0 92 124 90 734.8 0 90 125 78 734.8 0 78 126 58 734.8 0 58 127 70 734.8 0 70 128 92 734.8 0 92 129 89 734.8 0 89 130 133 734.8 0 133 131 158 734.8 0 158 132 128 734.8 0 128 133 95 734.8 0 95 134 72 734.8 0 72 135 146 734.8 0 146 136 190 734.8 0 190 137 176 734.8 0 176 138 100 734.8 0 100 139 103 734.8 0 103 140 86 734.8 0 86 141 119 734.8 0 119 142 118 734.8 0 118 143 130 734.8 0 130 144 139 734.8 0 139 145 93 734.8 0 93 146 92 734.8 0 92 147 228 734.8 0 228 148 183 734.8 0 183 149 148 734.8 0 148 150 133 734.8 0 133 151 123 734.8 0 123 152 148 734.8 0 148 153 161 734.8 0 161 154 112 734.8 0 112 155 210 734.8 0 210 156 295 734.8 0 295 157 246 734.8 0 246 158 260 734.8 0 260 159 183 734.8 0 183 160 157 734.8 0 157 161 167 734.8 0 167 162 157 734.8 0 157 163 151 734.8 0 151 164 104 734.8 0 104 165 91 734.8 0 91 166 136 734.8 0 136 167 217 734.8 0 217 168 229 734.8 0 229 169 146 734.8 0 146 170 156 734.8 0 156 171 162 734.8 0 162 172 149 734.8 0 149 173 100 734.8 0 100 174 92 734.8 0 92 175 266 734.8 0 266 176 555 734.8 0 555 177 279 734.8 0 279 178 127 734.8 0 127 179 114 734.8 0 114 180 94 734.8 0 94 181 113 734.8 0 113 182 133 734.8 0 133 183 159 734.8 0 159 184 192 734.8 0 192 185 121 734.8 0 121 186 239 734.8 0 239 187 228 734.8 0 228 188 281 734.8 0 281 189 153 734.8 0 153 190 100 734.8 0 100 191 85 734.8 0 85 192 123 734.8 0 123 193 108 734.8 0 108 194 111 734.8 0 111 195 106 734.8 0 106 196 95 734.8 0 95 197 82 734.8 0 82 198 73 734.8 0 73 199 116 734.8 0 116 200 151 734.8 0 151 201 99 734.8 0 99 202 70 734.8 0 70 203 93 734.8 0 93 204 149 734.8 0 149 205 122 734.8 0 122 206 112 734.8 0 112 207 110 734.8 0 110 208 119 734.8 0 119 209 136 734.8 0 136 210 136 734.8 0 136 211 129 734.8 0 129 212 145 734.8 0 145 213 143 734.8 0 143 214 181 734.8 0 181 215 168 734.8 0 168 216 238 734.8 0 238 217 198 734.8 0 198 218 140 734.8 0 140 219 158 734.8 0 158 220 185 734.8 0 185 221 329 734.8 0 329 222 550 734.8 0 550 223 520 734.8 0 520 224 286 734.8 0 286 225 266 734.8 0 266 226 191 734.8 0 191 227 208 734.8 0 208 228 176 734.8 0 176 229 170 734.8 0 170 230 133 734.8 0 133 231 79 734.8 0 79 232 136 734.8 0 136 233 121 734.8 0 121 234 151 734.8 0 151 235 131 734.8 0 131 236 174 734.8 0 174 237 127 734.8 0 127 238 191 734.8 0 191 239 125 734.8 0 125 240 135 734.8 0 135 241 313 734.8 0 313 242 344 734.8 0 344 243 306 734.8 0 306 244 196 734.8 0 196 245 208 734.8 0 208 246 164 734.8 0 164 247 195 734.8 0 195 248 323 734.8 0 323 249 350 734.8 0 350 250 107 734.8 0 107 251 59 734.8 0 59 252 201 734.8 0 201 253 198 734.8 0 198 254 288 734.8 0 288 255 339 734.8 0 339 256 228 734.8 0 228 257 168 734.8 0 168 258 120 734.8 0 120 259 84 734.8 0 84 260 77 734.8 0 77 261 71 734.8 0 71 262 77 734.8 0 77 263 109 734.8 0 109 264 157 734.8 0 157 265 172 734.8 0 172 266 147 734.8 0 147 267 179 734.8 0 179 268 479 734.8 0 479 269 622 734.8 0 622 270 162 734.8 0 162 271 182 734.8 0 182 272 136 734.8 0 136 273 177 734.8 0 177 274 225 734.8 0 225 275 158 734.8 0 158 276 74 734.8 0 74 277 58 734.8 0 58 278 79 734.8 0 79 279 102 734.8 0 102 280 198 734.8 0 198 281 146 734.8 0 146 282 160 734.8 0 160 283 219 734.8 0 219 284 132 734.8 0 132 285 119 734.8 0 119 286 181 734.8 0 181 287 331 734.8 0 331 288 180 734.8 0 180 289 109 734.8 0 109 290 72 734.8 0 72 291 95 734.8 0 95 292 147 734.8 0 147 293 237 734.8 0 237 294 158 734.8 0 158 295 86 734.8 0 86 296 108 734.8 0 108 297 72 734.8 0 72 298 98 734.8 0 98 299 110 734.8 0 110 300 166 734.8 0 166 301 111 734.8 0 111 Finished in 1.24 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 31,869 Reads with adapters: 2,111 (6.6%) Reads that were too short: 24 (0.1%) Reads written (passing filters): 2,087 (6.5%) Total basepairs processed: 7,006,299 bp Total written (filtered): 574,357 bp (8.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2111 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 14 0.0 3 0 13 0 1 17 1642 0.0 3 1576 58 5 3 18 8 0.0 3 1 6 1 19 240 0.0 3 226 10 1 3 20 160 0.0 3 158 2 21 1 0.0 3 0 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 1 30 1 0.0 3 0 0 0 1 36 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (29 us/read; 2.09 M reads/minute). === Summary === Total reads processed: 2,087 Reads with adapters: 56 (2.7%) Reads written (passing filters): 2,087 (100.0%) Total basepairs processed: 574,357 bp Total written (filtered): 574,186 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 56 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.6% C: 19.6% G: 35.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 54 32.6 0 54 4 1 8.2 0 1 5 1 2.0 0 1